The lentivirus was collected after 48 h transfection. For the viral transduction, 786-O cells were plated in 6-well plate until ... Then, the membranes were blocked with 5% Albumin from bovine serum (BSA) and incubated in primary antibodies against targeted ... Lentiviral constructs, lentivirus production, and infection. The wild type (WT) VHL was cloned into the Tet-one vector with ... The medium was replaced with indicated lentivirus-containing medium and incubated for 24 h. 2 µg /ml puromycin (Sigma) was used ...

Lentivirus, stable cell line construction and quantitative real-time PCR. The lentiviral vector system and empty vector were ... The cell lines were cultured in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS) and 1% ... 2F-I). The knockdown efficiency of UCK2 by lentivirus was detected using quantitative real-time PCR (Additional file 2: Fig. ... The knockdown efficiency of UCK2 by lentivirus was detected using quantitative real-time PCR; (D) gene expression of SULF2 in ...

E) HUVECs were transduced with lentivirus encoding TF, TFshort, or vector control and cells were analyzed for their ability to ... Controls include treatment with bovine serum albumin (BSA), a TF inhibitory antibody (anti-TF) or its isotype control, or ... HEK293T cells (ATCC) were cultured in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (HyClone). Cells were ... 5 mM CaCl2 1 mg/mL bovine serum albumin) that was then replaced with HBS-BSA containing factor VIIa (0.3-0.6 nM, Haematologic ...

Lentivirus transduction and preparation. Defective-lentivirus shares had been ready as defined previously (66). In short, 1.5 ... The cells had been refed 24 h posttransfection with clean DMEM filled with 10% fetal bovine serum, and the transfection ... Lentiviruses released into the 1166393-85-6 manufacture tradition press had been harvested at 48 or 72 l posttransfection, ... For shRNA knockdown of miR-21, HEK293T or U-251MG cells had been transduced with lentiviruses (MOI = 10) extracted from pLKO.1- ...

All cells were cultured in DMEM with 10% bovine calf serum (Invitrogen) at 5% CO2. After lentivirus transfection, cells were ...

Het-1A (3×105) cells were transduced with the lentivirus carrying KLF5 or the noncoding sequence using polybrene (5 µg/ml). At ... After blocking with 1% bovine serum albumin in PBS, the cells were incubated in rabbit anti-human monoclonal antibody serum as ... 48 hours after infection, 2 µg/ml puromycin was added to the media to select for lentivirus-infected cells. Real-time PCR and ...

Lentiviruses, Bovine B4.909.777.731.589.500 Lentiviruses, Equine B4.909.777.731.589.520 Lentiviruses, Feline B4.909.777.731. ... Bovine Viral B4.909.777.310.700.150.100 Diarrhea Virus 2, Bovine Viral B4.909.777.310.700.150.120 Diarrhea Viruses, Bovine ... 589.530 Lentiviruses, Ovine-Caprine B4.909.777.731.589.600 Lentiviruses, Primate B4.909.777.731.589.650 Leonurus B1.650.940.800 ... Bovine B4.909.574.807.200.600 B4.613.807.200.600 B4.909.777.731.200.600 Leukemia Virus, Feline B4.909.574.807.375.500 B4.613. ...

Lentiviruses, Bovine B4.909.777.731.589.500 Lentiviruses, Equine B4.909.777.731.589.520 Lentiviruses, Feline B4.909.777.731. ... Bovine Viral B4.909.777.310.700.150.100 Diarrhea Virus 2, Bovine Viral B4.909.777.310.700.150.120 Diarrhea Viruses, Bovine ... 589.530 Lentiviruses, Ovine-Caprine B4.909.777.731.589.600 Lentiviruses, Primate B4.909.777.731.589.650 Leonurus B1.650.940.800 ... Bovine B4.909.574.807.200.600 B4.613.807.200.600 B4.909.777.731.200.600 Leukemia Virus, Feline B4.909.574.807.375.500 B4.613. ...

Lentiviruses, Bovine B4.909.777.731.589.500 Lentiviruses, Equine B4.909.777.731.589.520 Lentiviruses, Feline B4.909.777.731. ... Bovine Viral B4.909.777.310.700.150.100 Diarrhea Virus 2, Bovine Viral B4.909.777.310.700.150.120 Diarrhea Viruses, Bovine ... 589.530 Lentiviruses, Ovine-Caprine B4.909.777.731.589.600 Lentiviruses, Primate B4.909.777.731.589.650 Leonurus B1.650.940.800 ... Bovine B4.909.574.807.200.600 B4.613.807.200.600 B4.909.777.731.200.600 Leukemia Virus, Feline B4.909.574.807.375.500 B4.613. ...

Lentiviruses, Bovine B4.909.777.731.589.500 Lentiviruses, Equine B4.909.777.731.589.520 Lentiviruses, Feline B4.909.777.731. ... Bovine Viral B4.909.777.310.700.150.100 Diarrhea Virus 2, Bovine Viral B4.909.777.310.700.150.120 Diarrhea Viruses, Bovine ... 589.530 Lentiviruses, Ovine-Caprine B4.909.777.731.589.600 Lentiviruses, Primate B4.909.777.731.589.650 Leonurus B1.650.940.800 ... Bovine B4.909.574.807.200.600 B4.613.807.200.600 B4.909.777.731.200.600 Leukemia Virus, Feline B4.909.574.807.375.500 B4.613. ...

Lentiviruses, Bovine B4.909.777.731.589.500 Lentiviruses, Equine B4.909.777.731.589.520 Lentiviruses, Feline B4.909.777.731. ... Bovine Viral B4.909.777.310.700.150.100 Diarrhea Virus 2, Bovine Viral B4.909.777.310.700.150.120 Diarrhea Viruses, Bovine ... 589.530 Lentiviruses, Ovine-Caprine B4.909.777.731.589.600 Lentiviruses, Primate B4.909.777.731.589.650 Leonurus B1.650.940.800 ... Bovine B4.909.574.807.200.600 B4.613.807.200.600 B4.909.777.731.200.600 Leukemia Virus, Feline B4.909.574.807.375.500 B4.613. ...

Proliferation, development, inflammation, and apoptosis in STAD are observed using sh-ASCL2 lentivirus via TLR4 activation in ... ASCL2 knockdown lentivirus administration. The ASCL2 knockdown lentivirus (sh-ASCL2) and control lentivirus are designed and ... GES-1 cells are maintained in RPMI-1640 (Thermo Fisher Scientific, Inc., MA, USA) with 10% fetal bovine serum at 37 °C in a ... Table 1 The name and sequence of ASCL2 lentivirus. Full size table. ...

Lentiviruses were produced as previously explained29 based on the protocol for the amplification of second generation ... fetal bovine serum (Invitrogen, Carlsbad, USA) as well as penicillin/streptomycin (Lonza, Basel, Switzerland) were transfected ... Narciclasine were crossed with mice were infected by lentiviruses expressing Cre recombinase to obtain STIM1/2- deficient ... PmLF of the second passage were seeded at 1.5 105 cells per well of a 6-well plate and infected by lentiviruses expressing Cre ...

Lentivirus transfection and flow sorting. HUVEC cells (LONZA #CC-2519) were grown in EBM™ Basal medium (LONZA, #CC-3121) ... supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). HUVEC cells were co-infected with various ... with BamHI sites by Gibson Assembly to package lentiviruses (Pregen plasmid: ZP509). The aa sequence of GCN4 used was ... with AgeI and RsrII sites by Gibson Assembly to package lentiviruses (Pregen plasmid: ZP512). Once expressed, the scFv GCN4 ...

Immunoblot of equal amounts of MK-8998 protein from cells infected with lentivirus expressing shRNA targeting Xrn2 or a ... fetal bovine serum at 37?C in a humidified atmosphere of 5% CO2. GFP vector construction and lentiviral transduction KB and S1 ... 100 g/mL bovine pituitary draw out (Biomedical Systems, Stoughton, MA), 20 g/mL ascorbic acidity (Sigma, St. Louis, MI), 200 mg ... cells were transfected with lentivirus vectors carrying Amlexanox green fluorescent protein (GFP). The GFP sequence was cloned ...

C1QBP regulates hypoxanthine level in RCC. ACHN and 786-O cells were transduced with lentivirus-mediated Scr control, shC1QBP ... fetal bovine serum (HAKATA, China) and 1 × penicillin/streptomycin (Biological Industries, Israel) in a humidified 37°C ... Then 786-O and ACHN cells were infected with the lentivirus supernatant respectively for 48 h and selected for 10 days with 2 ... Luciferase-labeled C1QBP overexpression and control of ACHN cells were stably constructed through lentivirus-mediated ...

We will concentrate at first on m6A and m5C post-transcriptional modifications, and on three retroviral genera: lentiviruses ( ... human T-lymphotropic virus and bovine leukemia virus), and the oncogenic gammaretrovirus (MLV, mouse leukemia virus). In order ...

We will concentrate at first on m6A and m5C post-transcriptional modifications, and on three retroviral genera: lentiviruses ( ... human T-lymphotropic virus and bovine leukemia virus), and the oncogenic gammaretrovirus (MLV, mouse leukemia virus). In order ...

Willett, B.J., Hosie, M.J. , Neil, J.C. , Turner, J.D. and Hoxie, J.A. (1997) Common mechanism of infection by lentiviruses. ... Atypical bovine morbillivirus infections in the Serengeti ecosystem. Biotechnology and Biological Sciences Research Council ... Willett, B.J., Hosie, M.J. , Neil, J.C. , Turner, J.D. and Hoxie, J.A. (1997) Common mechanism of infection by lentiviruses. ... Dietrich, I., McEwan, W. A., Hosie, M. J. and Willett, B. J. (2011) Restriction of the felid lentiviruses by a synthetic feline ...

Stable knockdown of TFE3 in MCF7 FLCN-KO breast cancer cells was achieved using the Mission lentivirus shRNA empty vector (shEV ... fetal bovine serum (FBS) (Wisent, 080-150), 100 U/mL penicillin plus 100 μg/mL streptomycin (Wisent, 450-201-EL), and 50 μg/mL ... Lentivirus was produced for the FLCN targeting sequences as well as an empty lentiCRISPR vector for control lines. Lentiviral ... cells were assayed for their ability to process dye-quenched bovine serum albumin (DQ-BSA). DQ-BSA is a self-quenched ...

To further explore the role of LEF1 in the CSC-like phenotype, we applied a lentivirus-based approach to stably express LEF1(LV ... heat-inactivated foetal bovine serum (Gibco-BRL) and antibiotics (100 U/ml penicillin and 100 U/ml streptomycin, HyClone ... Vector construction and lentivirus infection. We constructed lentiviral vectors encoding the human LEF1 gene or green ...

1 day after transduction with lentivirus, the moderate was became the monocyte moderate, comprising RPMI1640 (Wako, Japan) and ... fetal bovine serum supplemented with least essential moderate alpha (MEM, Wako, Japan) MLN8054 at 5% CO2 and 37C. ... accompanied by lentivirus creation (S1 Fig, series available on demand). Since each gene series is certainly spaced by polyA ... 20 ng/ml rhM-CSF(Wako, Japan), 20 ng/ml IL-4 (Wako, Japan), 1 mM sodium pyruvate, 50 M ?-mercaptoethanol, and 10% fetal bovine ...

Bovine foamyvirus, Bovine herpesvirus 4 (BoHV-4) - Movar virus, Bovine immunodeficiency virus, Bovine lentivirus group, Bovine ... Feline Lentivirus, Feline Leukemia, Feline Panleukopenia, Feline Parvovirus, Feline Sarcoma virus, Bovine adenovirus 4,5,8, ... leukemia virus, Bovine parvovirus, Bovine torovirus, Caprine arthritis encephalitis virus, Goat adenovirus 1, Ovine adenovirus ... Equine lentivirus, Equine Torovirus, Feline Calicivirus, Feline Coronavirus, Feline Foamyvirus, Feline Immunodeficiencyvirus, ...

... w/v fatty acid-free bovine serum albumin (BSA) and 0.5 mM oleate. Meanwhile, HepG2 cells were infected with lentivirus of short ... HFD-fed mice were injected with lentivirus of sh-lncARSR or sh-NC. a LncARSR expression in mice detected by RT-qPCR. b Lipid ... Oleate-treated HepG2 cells were infected with lentivirus of sh-lncARSR, sh-NC, oe-lncARSR or oe-NC. a Expression of YAP1, IRS2 ... n = 15 *p , 0.05 against NAFLD mice injected with sh-NC lentivirus or sh-NC-transfected HepG2 cells. Differences between two ...

... that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus ... The bovine field isolate J1961 (major Type II) and the ovine field isolate JIII-86 (Type III) were compared with the laboratory ... Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and PF- ... The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The ...

Treatment of bovine MDSCs with ß-catenin inhibitor and activator showed that the Wnt/ß-catenin pathway is involved in bovine ... We isolated primary rumen epithelial cells from a steer through trypsin digestion and transduced them with lentiviruses ... However, the role of WDR13 in bovine cells is unclear. Here, we investigated the effect of WDR13 on bovine skeletal muscle- ... RESULTS: Podocan protein expression increased significantly during bovine MDSC differentiation. Differentiation of bovine MDSC ...

T24, UMUC3 and HEK 293 T cells were cultured with Dulbeccos modified eagles medium (Gibco, USA) containing 10% foetal bovine ... The role of circFAM13B in BCa was investigated by transfecting circFAM13B overexpression or knockdown lentivirus in T24 and ... Once fixed, cells were permeabilized with 0.1% Triton X-100 (Solarbio, Beijing, China). After blocking with 3% bovine serum ... CircFAM13B knockdown and overexpression lentivirus vectors were acquired from HANBIO (HANBIO, Shanghai, China). T24 or UMUC3 ...

... provides a wide variety of tests for various Bovine and Swine diseases. ... With a variety of diagnostics available for bovine, ovine, and swine, Neogen has the veterinary applications necessary to ... Antibody detection test for Small Ruminent Lentiviruses: Ovine Progressive Pneumonia (OPP) and Caprine Arthritis Encephalitis ( ... Neogen offers veterinary diagnostics proficient in virus detection within bovine, ovine, and swine applications. Neogen is a ...

... fetal bovine serum (FBS; Atlanta Biologicals). Plasmids and Lentivirus Creation gRNAs targeting specific genes had been ... and useful for lentivirus creation (3, 16). Lentivirus titer was established as previously referred to (16). Cas9-expressing ... The lentivirus creation treatment continues to be referred to (3 previously, 16). Genome-Wide Tumor Vulnerability and Level of ... K562 cells had been transduced with GeCKO V2 lentivirus libraries at a minimal MOI of 0.3 and decided on in puromycin for seven ...

C) Section through a bovine herpesvirion. Images in (B) and (C) were kindly provided by Dr. Peter Wild. ... In the lentiviruses, of which HIV is a member, the cap-. and D show electron micrographs of budding virus particles ... 1995) p. 114. (B) Negatively stained electron micrograph of an intracellular particle of bovine herpesvirus. ... D) Electron micrographs of bovine immunodeficiency virus. Top: budding particles; bottom: mature extracytoplasmic ...