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*  Laminin in the male germ cells of Drosophila. | JCB
... the entire Drosophila laminin complex, or against the mouse laminin complex and against laminin A and B1 chains with specific ... The axonemes of elongating spermatids react with antibodies against the Drosophila laminin B1, B2 and laminin A chains. The ... Laminin in the male germ cells of Drosophila.. F Wang, M Hanske, K Miedema, G Klein, P Ekblom, W Hennig ... Transcripts of laminin B2 were detected in the RNA of male germ cells with the polymerase chain reaction and by in situ ...
http://jcb.rupress.org/content/119/4/977
*  α3β1 Integrin Is Required for Normal Development of the Epidermal Basement Membrane | JCB
Preparation of Laminin-5-rich Extracellular Matrix from Keratinocytes. To prepare laminin-5-rich ECM, human epidermal ... We are grateful to Robert Burgeson for providing us with purified laminin-5 and antisera to laminin-5 and type VII collagen, as ... Entactin/nidogen localizes to the lamina densa along with type IV collagen, laminin-5, and other laminin isoforms, including ... and anti-laminin-5 serum (Fig. 7, A-D, G, and H). Laminin-5 was not detected in the unstratified skin of E11.5 embryos (data ...
http://jcb.rupress.org/content/137/3/729
*  Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers in Vivo | JCB
Laminin 2.. We provide evidence here that both muscle and neural agrin bind to laminin containing α2-chain in vivo. Agrin ... 1999). Laminin isoforms 2 and 4 are predominant laminin isoforms of basal lamina and are expressed in developing muscle fibers ... Neural and Muscle Agrin Bind to Laminin. To examine whether the agrin binding to laminin could be responsible for homogenous ... 1999) Interaction of agrin with laminin requires a coiled-coil conformation of the agrin-binding site within the laminin g1 ...
http://jcb.rupress.org/content/153/7/1441
*  Animal Models for Muscular Dystrophy Show Different Patterns of Sarcolemmal Disruption | JCB
... which is presumed to be involved in self-aggregation of laminin-2 heterotrimers, as in the case of laminin 1 (Schittny and ... 1994b) Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene. Nat Genet 8:297-302, pmid:7874173.. ... 1990) Terminal short arm domains of basement membrane laminin are critical for its self-assembly. J Cell Biol 110:825-832, pmid ... 1997) Mild congenital muscular dystrophy in two patients with an internally deleted laminin α2-chain. Hum Mol Genet 6:747-752, ...
http://jcb.rupress.org/content/139/2/375?ijkey=5a3d2dda3770ad214577d1a60027e938a0b313ee&keytype2=tf_ipsecsha
*  Oxidative status of muscle is determined by p107 regulation of PGC-1α | JCB
C) Type I MyHC (red), Laminin (green), and DAPI (blue) fluorescence immunostaining of representative TA sections for wild-type ... P, Flag-p107; S, empty vector (sham). (C) Type IIa MyHC (red) and Laminin (green) fluorescence immunostaining of representative ... and rabbit anti-Laminin (Sigma-Aldrich). Secondary antibodies used were Alexa Fluor 488, Alexa Fluor 568, and Alexa Fluor 647 ...
http://jcb.rupress.org/content/190/4/651
*  NF-κB Mediates αvβ3 Integrin-induced Endothelial Cell Survival | JCB
... but not when cells were plated on fibronectin and laminin (data not shown). Ha2/5 antibody was able to inhibit laminin-induced ... C) RAECΔN2 clone was plated on vitronectin (VN), fibronectin (FN), laminin (LM), collagen type I (Coll I), and F11 monoclonal ... Osteopontin was used at 100 nM; fibronectin, vitronectin, and laminin at 50 nM; collagen type I at 5 μg/ml; and F11 at 500 μg/ ... In contrast, RAECΔN2 cells plated on fibronectin, laminin, and collagen type I continued to be protected (Fig. 9 C). Thus, ...
http://jcb.rupress.org/content/141/4/1083?ijkey=c1a42226ec6cdecce3920fa4ff9b21a42bda6c8d&keytype2=tf_ipsecsha
*  N-syndecan deficiency impairs neural migration in brain | JCB
Petri dishes were coated by 20 μg/ml poly-l-lysine, 50 μg/ml HB-GAM, 10 μg/ml laminin, or 100 μg/ml BSA. After 24 h, cells were ... 7 E). This indicates that laminin does not induce such a strong clustering of N-syndecan and EGFR and that the HB-EGF-induced ... It is noteworthy that although binding to the HB-GAM matrix alone induces the clustering of N-syndecan and EGFR, laminin matrix ... HB-EGF further enhances the receptor clustering on HB-GAM but not as strongly on laminin-plated cells, which, again, speaks for ...
http://jcb.rupress.org/content/174/4/569
*  Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells | JCB
The cells on the surface of acini are in contact with ECM proteins, such as laminin V (Fig. 2 D), and the basal surface of ... 2 D). Cells tracking along ECM proteins, such as the laminin found in the basement membrane on the basal surface of MCF-10A ... Acini were then fixed and immunostained with α-laminin V (green) and α-α6 integrin (red) antibodies and counterstained with ... α6 integrin and laminin V (Chemicon); phosphorylated MLC2S19 (mouse), phosphorylated MLC2S19 (rabbit), phosphorylated MLC2T18/ ...
http://jcb.rupress.org/content/179/7/1555
*  Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia | JCB
left panels) x-y images showing the top of the BM immunostained for laminin (left) or collagen IV (right). (right panels) x-z ... Laminin (Invitrogen) was labeled with Alexa Fluor 488 with a protein labeling kit (Invitrogen) according to the manufacturer's ... 1 mix of 9-10 mg/ml Matrigel with 0.5 mg/ml of labeled or unlabeled laminin for 1 h at 4°C. 75 µl was then removed, and the ... BM was detected by laminin staining (red). Merged images are shown in the right column. HT29 (B) and HCT116 (C) cells are shown ...
http://jcb.rupress.org/content/189/3/541.full
*  Delayed Retraction of Filopodia in Gelsolin Null Mice | JCB
A sequence of VEC-DIC video images of a hippocampal growth cone from E17 Gsn− mice growing on laminin-coated substrate. The ... 1989) Transient expression of laminin immunoreactivity in the developing rat hippocampus. J Neurocytol 18:451-463, pmid:2509639 ... 1.5; German) coated with laminin (20 μg/ml; Sigma Chemical Co.). The cells were cultured in a humidified 5% CO2 incubator at 37 ... Embryonic day 17 hippocampal cells from wt and Gsn− mice were dissociated and cultured on laminin-coated coverslips for 1 to 3 ...
http://jcb.rupress.org/content/138/6/1279?ijkey=3b6aa7f7cfc1f3d700fe7bae12311325ca85c479&keytype2=tf_ipsecsha
*  Regulation of alpha 6 beta 1 integrin laminin receptor function by the cytoplasmic domain of the alpha 6 subunit. | JCB
Regulation of alpha 6 beta 1 integrin laminin receptor function by the cytoplasmic domain of the alpha 6 subunit.. L M Shaw, A ... The alpha 6A-delta CYT transfectants adhered to laminin, however, when Ca++/Mg++ was replaced with 150 microM Mn++. We also ... P388D1 transfectants which expressed these serine mutations adhered to laminin in response to PMA to the same extent as cells ... Regulation of alpha 6 beta 1 integrin laminin receptor function by the cytoplasmic domain of the alpha 6 subunit. ...
http://jcb.rupress.org/content/123/4/1017.long
*  Reversion of the Malignant Phenotype of Human Breast Cells in Three-Dimensional Culture and In Vivo by Integrin Blocking...
1991) Co-ordinate expression of the α6 integrin laminin receptor sub-unit and laminin in breast cancer. J Pathol 165:213-220, ... 1988b) Laminin receptor on platelets is the integrin VLA-6. Nature (Lond) 336:487-489, pmid:2973567.. ... 1994) A novel laminin E8 cell adhesion site required for lung alveolar formation in vitro. J Cell Biol 124:1083-1090, pmid: ... 1996) Signal transduction by the α6β4 integrin: charting the path between laminin binding and nuclear events. J Cell Sci 109: ...
http://jcb.rupress.org/content/137/1/231
*  Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem...
ES cell mutants to laminin is consistent with the fact that they spread less well on laminin than fibronectin. ... Laminin receptors are all members of the β1 integrin family (Newham and Humphries, 1996), and reduced adhesion of the talin ... The ability of wild-type ES cells, and the various talin ES cell mutants to adhere to fibronectin and laminin is shown in Fig. ... 3 B). The morphology of the talin (−/−) ES cell mutants plated on laminin was similar to that on gelatin with a marked ...
http://jcb.rupress.org/content/142/4/1121?ijkey=8de9091b44ac9f7788766d2c41775e10242926a8&keytype2=tf_ipsecsha
*  lncRNA Chronos is an aging-induced inhibitor of muscle hypertrophy | JCB
Mouse anti-laminin antibodies were purchased from Sigma-Aldrich. Mouse anti-myosin (MF20) antibody was purchased from the ... Immunofluorescence labeling of laminin and quantification of TA myofiber cross sections indicated siChronos treatment resulted ... and siNC1 were analyzed by immunofluorescence staining of laminin (Fig. 4 A). In agreement with earlier experiments (Fig. 2 C ...
http://jcb.rupress.org/content/early/2017/08/29/jcb.201612100
*  Role of Cholesterol in Formation and Function of a Signaling Complex Involving αvβ3, Integrin-Associated Protein (Cd47), and...
A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptorBiochemistry.2619876896 ... 1987) YIGSR, a synthetic laminin pentapeptide, inhibits experimental metastasis formation. Science. 238:1132-1134, pmid:2961059 ... Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor bindingCell.481987989 ... 1 A). These control beads bind to a nonintegrin laminin receptor (Graf et al. 1987a) expressed on OV10 cells. ...
http://jcb.rupress.org/content/146/3/673
*  Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin | JCB
DG and laminins are required for proper clustering of Na+ channels at PNS nodes. Specific laminins (211 and 511) and dystrophin ... Both laminin and Schwann cell dystroglycan are necessary for proper clustering of sodium channels at nodes of Ranvier. J. ... A laminin-2, dystroglycan, utrophin axis is required for compartmentalization and elongation of myelin segments. J. Neurosci. ... Among those, specific laminins and dystroglycan complexes contribute to Na+ channel clustering at peripheral nodes by unknown ...
http://jcb.rupress.org/content/208/3/313
*  Integrin α2β1 Is a Receptor for the Cartilage Matrix Protein Chondroadherin | JCB
1989) Endothelial cells use α2β1 integrin as a laminin receptor. J Cell Biol 109:2455-2462, pmid:2530239.. ... 1989) The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others. Proc Natl Acad ... α2β1 on platelets and melanoma cells bind collagen (27, 46), while α2β1 on other cell types binds both collagen and laminin (9 ... 1990) Integrin recognition of different cell-binding fragments of Laminin (P1, E3, E8) and evidence that α6β1 but not α6β4 ...
http://jcb.rupress.org/content/138/5/1159
*  Genetic Analysis of Collagen Q: Roles in Acetylcholinesterase and Butyrylcholinesterase Assembly and in Synaptic Structure and...
1995) Aberrant differentiation of neuromuscular junctions in mice lacking s-laminin/laminin β2. Nature 374:258-262, pmid: ... 1998) Synaptic laminin prevents glial entry into the synaptic cleft. Nature 393:698-701, pmid:9641682.. ... 1994) Collagen IV α3, α4, and α5 chains in rodent basal laminae: sequence, distribution, association with laminins, and ... laminin-11 (Patton et al., 1998). ColQ might exert a similar effect. In addition, compensatory changes in quantal size and ...
http://jcb.rupress.org/content/144/6/1349
*  Expression of the Heparan Sulfate Proteoglycan, Perlecan, during Mouse Embryogenesis and Perlecan Chondrogenic Activity In...
Laminin, rabbit anti-mouse laminin, fibronectin, collagen type IV (Col IV), rabbit anti-mouse collagen type IV, Matrigel, Pln, ... Laminin and collagen type IV are not expressed in cartilage primordia. Frozen sections of d 15.5 embryos were probed with ... Laminin and collagen type IV, other basal lamina proteins commonly found colocalized with Pln, are absent from the cartilage ... In contrast, laminin has been found in human nasal cartilage [SunderRaj et al., 1995].) A hallmark of chondrocyte ...
http://jcb.rupress.org/content/145/5/1103
*  miR669a and miR669q prevent skeletal muscle differentiation in postnatal cardiac progenitors | JCB
Laminin expression in miRdsRed (B)- and miR669a (E)-treated muscle sections. miR669a-treated muscles showed smaller fibers ... C, C′, F, and F′) Laminin and β-galactosidase expression on miRdsRed (C and C′)- and miR669a (F and F′)-injected muscle ... Immunofluorescence analysis for Cx43/GFP on heart sections and laminin/GFP on muscle sections clearly showed that H4 KO/GFP ... TA muscles were analyzed for the expression of laminin and GFP. GFP-positive cells were specifically localized and integrated ...
http://jcb.rupress.org/content/193/7/1197
*  Apical targeting of syntaxin 3 is essential for epithelial cell polarity | JCB
Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly. Nat. Cell Biol. 3:831-838. ...
http://jcb.rupress.org/content/173/6/937
*  A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity | JCB
Rac1 orientates epithelial apical polarity through effects on basolateral laminin assembly. Nat. Cell Biol. 3:831-838. ... as laminin is required for correct apical pole orientation in three-dimensional epithelial cysts (O'Brien et al., 2001) and ...
http://jcb.rupress.org/content/178/2/323
*  Serum response factor is crucial for actin cytoskeletal organization and focal adhesion assembly in embryonic stem cells | JCB
In contrast, adhesion to laminin and collagen IV was significantly reduced by ∼50% in Srf(−/−) ES cells as compared with Srf(−/ ... C) Srf(−/−) ES cells display adhesion defects on laminin and collagen IV. ES cells of the indicated Srf genotype were ... Laminin (mouse) and fibronectin (calf) were purchased by Harbor Bio Products and collagen IV was purchased by GIBCO BRL. ... 96-well plates were coated with 25 μg/ml fibronectin, 25 μg/ml laminin or 50 μg/ml collagen IV at 4°C overnight. To avoid ...
http://jcb.rupress.org/content/156/4/737
*  Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly...
The capability of the integrin VLA-3 to function as a receptor for collagen (Coll), laminin (Lm), and fibronectin (Fn) was ... Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly- ... Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly- ...
http://jcb.rupress.org/content/112/1/169
*  Modulation of chromatin position and gene expression by HDAC4 interaction with nucleoporins | JCB
NRVMs plated on laminin-coated coverslips were loaded with 5 µmol/L Indo-1. Individual cells were excited at 350 nm and the ...
http://jcb.rupress.org/content/193/1/21