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*  Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus | Microbial...
To generate a simplified signal sequence, we substituted a part of the yGLuc hydrophobic core with repeats of a single amino acid. The VLFALICI sequence was initially substituted to contain an eight residue repeat of a single amino acid (Figure 3). L8, M8, W8, and F8 increased secreted protein activity but repeats containing other residues (I, T, S, Q, Y, A, V, and C) did not. This result indicated that a complex amino acid sequence such as VLFALICI can be substituted with a repeat of select, single amino acids. Furthermore, although eight residue repeats of the residues I, T, S, Q, Y, A, V, and C, appeared not to be suitable, or too weak for a hydrophobic core; in fact, a hydrophobic core consisting of I12 and I13 was able to function as a signal sequence (Figure 5a). Therefore, the hydrophobic core in a signal sequence can be determined by the number of hydrophobic amino acids without including charged amino acids such as E, D, R, and K. Moreover, the efficiency of secretory production can be ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0203-y
*  Direct fermentation of raw starch using a Kluyveromyces marxianus strain that expresses glucoamylase and Alpha-amylase to...
Wang, R., Wang, D., Gao, X. and Hong, J. (2014), Direct fermentation of raw starch using a Kluyveromyces marxianus strain that expresses glucoamylase and Alpha-amylase to produce ethanol. Biotechnol Progress, 30: 338-347. doi: 10.1002/btpr.1877 ...
http://onlinelibrary.wiley.com/doi/10.1002/btpr.1877/references
*  Synergistic effect of thioredoxin and its reductase from Kluyveromyces marxianus on enhanced tolerance to multiple...
Under certain conditions, ROS generated from cell metabolism causes more cell damages, and ROS generally includes super oxide, peroxide, and hydroxyl radical et al. [32]. In order to prevent from ROS damages, microorganisms build a perfect defense system, which involves some essential oxidoreductases and compounds, such as peroxiredoxins (Prxs), thioredoxin (TRX) and its reductase (TrxR), glutathione and its reductase (GLR) and peroxidase (GPX), catalase (CTT1), and superoxide dismutase (SOD) (Fig. 7a). These oxidoreductases might be also related to the tolerance to some specific inhibitors [32, 33]. Actually, the peroxiredoxin, encoded by KmTPX1, has been proved to contribute to an obvious enhancement of tolerance to both oxidative stress and lignocellulose-derived inhibitors [29]. Therefore, to further explore the potential functions of oxidoreductases on cells survival under tough conditions, the thioredoxin system from K. marxianus, composed of thioredoxin (KmTrx) and its reductase (KmTrxR), ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0795-5
*  Influence of carbon source and surface hydrophobicity on the aggregation of the yeast Kluyveromyces bulgaricus. - Semantic...
Aggregation of the yeast Kluyveromyces bulgaricus is mediated by the galactose-specific lectin KbCWL1. This lectin contains hydrophobic amino acids and its activity is calcium dependent. A specific fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid in the free acid form (ANS; Sigma Chemical Co., St. Louis, Missouri), was used to study the hydrophobic areas on the cellular surface of K. bulgaricus. Changes in surface hydrophobicity during the growth and aggregation of yeast cells were studied. Surface hydrophobicity increased during growth and depended on the amount of yeast cells in the culture medium. During growth, the size of the hydrophobic areas on the cell surface was measured using ANS and was found to increase with the percentage of flocculating yeasts. Our results strongly suggest that the hydrophobic areas of the cell walls of yeast cells are involved in the aggregation of K. bulgaricus.
https://www.semanticscholar.org/paper/Influence-of-carbon-source-and-surface-hydrophobic-Thiebault-Coulon/f317ae0a3fc5c6fb4864bf308e84cc59301bd38f
*  Simulation of process conditions of continuous ethanol fermentation of whey permeate using alginate entrapped Kluyveromyces...
Marwaha, S.S.; Kennedy, J.F.; Sehgal, V.K., 1988: Simulation of process conditions of continuous ethanol fermentation of whey permeate using alginate entrapped Kluyveromyces marxianus NCYC 179 cells in a packed-bed reactor system
https://eurekamag.com/research/001/684/001684414.php
*  中国科学院大连化学物理研究所机构知识库(DICP OpenIR): SECRETORY EXPRESSION OF THE EXO-INULINASE FROM K. MARXIANUS CBS 6556 IN PICHIA PASTORIS
Inulin is linear oligosaccharides [1]. Although inulin can be hydrolyzed to monosaccharides non-enzymatically by acid treatment, inhibitory by-products produced that complicate downstream biological process. Therefore, it is pivotal to establish a cost-effective inulinase (EC 3.2.1.7) production. In this study, we isolated the DNA sequence encoding the mature peptide sequence of the exo-inulinase gene from Kluyveromyces marxianus CBS 6556 and expressed in Pichia pastoris X-33. The purified recombinant enzyme (reINU) gave a specific activity of 13100 U•mg−1, which was about 100-fold higher that reported for the wild type protein isolated from K. marxianus CBS6556 [2, 3]. Ferguson plot analysis showed that secretion expressed reINU was a 169 kDa dimer. Detailed analytical assays showed that the optimal temperature and pH for reINU were 60 oC and pH 4.0, respectively. It was found that hydrolysis activity was about 20% higher in the presence of Mn2+. reINU was stable for over 3 days at room ...
http://cas-ir.dicp.ac.cn/handle/321008/113172
*  Kluyveromyces bulgaricus yeast lectins. Isolation of two galac...
Kluyveromyces bulgaricus yeast lectins. Isolation of two galactose-specific lectin forms from the yeast cell wall.: Incubation of galactose treated Kluyveromyce
https://www.mysciencework.com/publication/show/kluyveromyces-bulgaricus-yeast-lectins-isolation-two-galactose-specific-lectin-forms-from-yeast-cell-wall-57312bdd
*  A comparative study on the biosorption characteristics of some yeasts for Remazol Blue reactive dye.
Abstract Biosorption capacities and rates of different kinds of dried yeasts (Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces marxianus, Candida sp., C. tropical..
https://www.omicsonline.org/references/a-comparative-study-on-the-biosorption-characteristics-of-some-yeasts-for-remazol-blue-reactive-dye-379721.html
*  pH increasing problem of yeast fermentation (under aerobic condition) - Microbiology - BioForum
pH increasing problem of yeast fermentation (under aerobic condition) - posted in Microbiology: Dear all, Recently I did consuming experiments with two monosaccharides (glucose and galactose) by Kluyveromyces marxianus (yeast) in the fermentor. The initial pH of medium is 7.0. I think it is normal that the pH dropped down to 6.5 after overnight incubation while stirring but without ventilation.But I also found that when the reaction is stirring with ventilation, the pH increases immediatel...
http://www.protocol-online.org/forums/topic/23769-ph-increasing-problem-of-yeast-fermentation-under-aerobic-condition/
*  Colonization of the gastrointestinal tract - Turval
Kluyveromyces marxianus fragilis B0399 is a lactic yeast with characteristics which are different from those of lactobacilli and of bifidobacteria, and of the yeast Saccharomyces. It has been used for some time now as a probiotic in the zootechnical field as well (Commission Regulation EC 773/06). It is a eucaryotic type of cell (Lachance M.A 1) endowed with an elevated lactasic enzymatic activity (β-galactosidase). It ferments lactose with the production of lactic acid. The enzymatic activity, in anaerobic conditions typical of the intestine, is of a homofermenting type, in that it transforms all the resulting glucose is lactic acid, without the production of gas (C02) (ex.Saccharomyces) (Vananuvat-Kinsella2 ,Wasserman-Hopkins-Porges3). This contributes in modulation the intestinal environment, rducing the pH. Furthermore, it has shown to be particularly resistant to the action of antibiotics (Voughan 4 ...
http://www.turval.com/research/humans_and_nutrition/verification-of-the-capacity-of-colonization-of-the-gastrointestinal-tract-in-healthy-subjects-after-the-utililization-of-the-lactic-yeast-kluyveromyces-marxianus-fragilis-b0399-through-examination-of-the-feces-trial-n-130-1?set_language=en
*  Nucleosome organization on Kluyveromyces lactis centromeric DNAs | IRIS Uniroma1
The preferential assembly of specialized nucleosomes on budding yeast centromeres can be due either to the higher stability of specialized centromeric nucleosomes and/or to the lower stability of canonical centromeric nucleosomes with respect to bulk nucleosomes. We have evaluated the thermodynamic stability of canonical nucleosomes, assembled on Kluyueromyces lactis centromeric DNAs, with a competitive reconstitution assay and a theoretical method recently developed by us. The results, obtained by both methods, show that all five known centromeric DNAs from K. lactis are able to organize canonical nucleosomes, characterized by higher stability with respect those of bulk DNA. With `footprinting' and theoretical prediction, based on sequence-dependent DNA elasticity, we have found that centromeric canonical nucleosomes are characterized by nucleosome dyad axis multiple positioning, rotationally phased. The isoenergetic nucleosome multiple positions are relevant in understanding the transition ...
https://iris.uniroma1.it/handle/11573/254516
*  Confidently Estimating the Number of DNA Replication Origins : Statistical Applications in Genetics and Molecular Biology
We present a method for estimating and providing a confidence interval for the number of DNA replication origins in the genome of the yeast Kluyveromyces lactis. The method requires an initial set of verified sites from which a position specific frequency matrix (PSFM) can be constructed. We further assume that we have access to a sparingly used experimental procedure which can verify the functionality of a few, but not all, computationally predicted sites. While our motivation comes from estimating the number of autonomously replicating sequences (ARSs), our method can also be applied to estimating the genome-wide number of functional transcription factor binding sites, where functionality is determined by experimental verification of the transcription factor binding event using, for example, ChIP data. The reliability of our method is demonstrated by correctly predicting the known number of Saccharomyces cerevisiae ARSs as well as the number of S. cerevisiae probes that bind to the ...
https://www.degruyter.com/view/j/sagmb.2010.9.1/sagmb.2010.9.1.1544/sagmb.2010.9.1.1544.xml
*  Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally...
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. ...
http://dare.uva.nl/search?metis.record.id=103443
*  LAC4 - Beta-galactosidase - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/P00723
*  Molecular biology of Kluyveromyces lactis | Springer for Research & Development
The yeast Saccharomyces cerevisiaeis probably the most thoroughly understood amongst eukaryotic organisms and an excellent model for the study of eukaryotic cells in general; indeed, the term...
https://rd.springer.com/chapter/10.1007/978-3-642-84625-0_9
*  HOG1 - Mitogen-activated protein kinase HOG1 - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL...
Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes (By similarity).
http://www.uniprot.org/uniprot/Q6CJA8
*  URA3 - Orotidine 5'-phosphate decarboxylase - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/P07922
*  PKAR - cAMP-dependent protein kinase regulatory subunit - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/Q6CPK7
*  KEGG PATHWAY: Citrate cycle (TCA cycle) - Kluyveromyces lactis
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
http://www.genome.jp/kegg-bin/show_pathway?kla00020+KLLA0E00683g
*  UniProt: F2Z6F9 KLULA
ID F2Z6F9_KLULA Unreviewed; 108 AA. AC F2Z6F9; DT 31-MAY-2011, integrated into UniProtKB/TrEMBL. DT 31-MAY-2011, sequence version 1. DT 25-OCT-2017, entry version 37. DE SubName: Full=KLLA0C03091p {ECO:0000313,EMBL:CAH01186.1}; GN ORFNames=KLLA0_C03091g {ECO:0000313,EMBL:CAH01186.1}; OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC OS 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces. OX NCBI_TaxID=284590 {ECO:0000313,Proteomes:UP000000598}; RN [1] {ECO:0000313,EMBL:CAH01186.1, ECO:0000313,Proteomes:UP000000598} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / RC WM37 {ECO:0000313,Proteomes:UP000000598}; RX PubMed=15229592; DOI=10.1038/nature02579; RG Genolevures; RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., RA Lafontaine I., ...
http://www.genome.jp/dbget-bin/www_bget?uniprot:F2Z6F9_KLULA
*  Patent US5302697 - Peptide and DNA sequences - Google Patents
Secretory leader sequences, for use in secreting heterologous polypeptides in yeast, are formed by fusing part of the human serum albumin pre-sequence or part of the Kluyveromyces lactis killer toxin pre-sequence to the Saccharomyces cerevisiae mating factor alpha-1 KEX2 cleavage recognition site. The resulting fusion leader sequences are: (a) H2 N-Met-Lys-Trp-Val-Ser-Phe-Ile-Ser-Leu-Leu-Phe-Leu-Phe-Ser-Ser-Ala-Tyr-Ser-Arg-Ser-Leu-Asp-Lys-Arg-COOH or (b) H2 N-Met-Asn-Ile-Phe-Tyr-Ile-Phe-Leu-Phe-Leu-Leu-Ser-Phe-Val-Gln-Gly-Ser-Leu-Asp-Lys-Arg-COOH Conservative variations are also encompassed.
http://www.google.com.au/patents/US5302697
*  J Bacteriol 147(1), 155-160, 1981 Publication Passport - StrainInfo
Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell ...
http://www.straininfo.net/publications/22650
*  Structure-function insights into direct lipid transfer between membranes by Mmm1-Mdm12 of ERMES | JCB
The endoplasmic reticulum (ER)-mitochondrial encounter structure (ERMES) physically links the membranes of the ER and mitochondria in yeast. Although the ER and mitochondria cooperate to synthesize glycerophospholipids, whether ERMES directly facilitates the lipid exchange between the two organelles remains controversial. Here, we compared the x-ray structures of an ERMES subunit Mdm12 from Kluyveromyces lactis with that of Mdm12 from Saccharomyces cerevisiae and found that both Mdm12 proteins possess a hydrophobic pocket for phospholipid binding. However in vitro lipid transfer assays showed that Mdm12 alone or an Mmm1 (another ERMES subunit) fusion protein exhibited only a weak lipid transfer activity between liposomes. In contrast, Mdm12 in a complex with Mmm1 mediated efficient lipid transfer between liposomes. Mutations in Mmm1 or Mdm12 impaired the lipid transfer activities of the Mdm12-Mmm1 complex and furthermore caused defective phosphatidylserine transport from the ER to ...
http://jcb.rupress.org/content/early/2017/12/22/jcb.201704119
*  UniProt: H2AN94 KAZAF
ID H2AN94_KAZAF Unreviewed; 850 AA. AC H2AN94; DT 21-MAR-2012, integrated into UniProtKB/TrEMBL. DT 21-MAR-2012, sequence version 1. DT 20-DEC-2017, entry version 27. DE RecName: Full=V-type proton ATPase subunit a {ECO:0000256,RuleBase:RU361189}; GN Name=KAFR0A04090 {ECO:0000313,EMBL:CCF55844.1}; GN ORFNames=KAFR_0A04090 {ECO:0000313,EMBL:CCF55844.1}; OS Kazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / OS CECT 1963 / NBRC 1671 / NRRL Y-8276) (Yeast) (Kluyveromyces OS africanus). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kazachstania. OX NCBI_TaxID=1071382 {ECO:0000313,EMBL:CCF55844.1, ECO:0000313,Proteomes:UP000005220}; RN [1] {ECO:0000313,EMBL:CCF55844.1, ECO:0000313,Proteomes:UP000005220} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / RC NRRL Y-8276 {ECO:0000313,Proteomes:UP000005220}; RX PubMed=22123960; ...
http://www.genome.jp/dbget-bin/www_bget?uniprot:H2AN94_KAZAF