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*  Artificial skin, Artificial Skin: The skin generated in vitro is in fact the, Biology
... this is because certain products from fibroblast cells facilitate the proliferation of keratinocytes. Keratinocytes develop to ... The keratinocytes are dissociated by treating the skin explants with trypsin. These cells are sophisticated in vessels the ... The bulk (Ca 90%) of epidermis is constituted by cells that called keratinocytes which generated the dead cells (corneocytes) ...
http://www.expertsmind.com/questions/artificial-skin-30115360.aspx
*  DGIST Scholar: Erythropoietin promotes hair shaft growth in cultured human hair follicles and modulates hair growth in mice
Keratinocyte; Keratinocytes; Ki 67 Antigen; Male; Messenger RNA; Mice; Mice, Inbred C57BL; Models, Animal; Mouse; Non-Human; ... Hair shaft growth was measured in the absence or presence of EPO and matrix keratinocyte proliferation was examined by Ki-67 ... Methods: EPOR expression in DPCs and follicular keratinocytes was examined by RT-PCR and immunoblot. Phosphorylation of EPOR ... EPO significantly promoted the growth of DPCs and elongated hair shafts with increased proliferation of matrix keratinocytes in ...
http://scholar.dgist.ac.kr/handle/20.500.11750/3509
*  Keratinocyte - Wikipedia
Since keratinocyte differentiation inhibits keratinocyte proliferation, factors that promote keratinocyte proliferation should ... Functional keratinocytes are needed for tympanic perforation healing. A sunburn cell is a keratinocyte with a pyknotic nucleus ... Within the epidermis keratinocytes are associated with other cell types such as melanocytes and Langerhans cells. Keratinocytes ... to the developing keratinocytes. Houben E, De Paepe K, Rogiers V (2007). "A keratinocyte's course of life". Skin Pharmacology ...
https://en.wikipedia.org/wiki/Keratinocyte
*  Keratinocyte transglutaminase - Wikipedia
1992). "Type I keratinocyte transglutaminase: expression in human skin and psoriasis". J. Invest. Dermatol. 99 (1): 27-34. doi: ... Keratinocyte transglutaminase is a transglutaminase enzyme. A deficiency is associated with ichthyosis lamellaris. Epidermal ... 1992). "Organization and evolution of the human epidermal keratinocyte transglutaminase I gene". Proc. Natl. Acad. Sci. U.S.A. ... 1995). "Mutations of keratinocyte transglutaminase in lamellar ichthyosis". Science. 267 (5197): 525-8. doi:10.1126/science. ...
https://en.wikipedia.org/wiki/Keratinocyte_transglutaminase
*  Keratinocyte | cell | Britannica.com
... with a group of neighbouring keratinocytes (keratin-synthesizing epidermal cells) into which its dendrites transfer pigment. ... The keratinocytes slowly move outward through the epidermis as they mature, and they eventually die and are… ... contains layers of cells called keratinocytes. Only the basal layer, next to the dermis, contains cells that divide. A number ... with a group of neighbouring keratinocytes (keratin-synthesizing epidermal cells) into which its dendrites transfer pigment. ...
https://www.britannica.com/science/keratinocyte
*  Melanoma cells talk to keratinocytes | Science
Thank you for your interest in spreading the word about Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://science.sciencemag.org/content/355/6330/1169.17/tab-pdf
*  keratinocyte cell culture
... Courtland Yockey courtland.yockey at mindspring.com Thu Jan 13 15:18:42 EST 2000 *Previous message: ... When last I interacted with them 2 years ago, their labs were doing primary keratinocyte cultures from human newborn foreskins ... I am interested in generating primary keratinocyte cell cultures and would appreciate any advice, references, protocols, and ...
http://www.bio.net/bionet/mm/cellbiol/2000-January/011997.html
*  Antigen presentation by keratinocytes directs autoimmune skin disease | PNAS
Keratinocytes as APCs.. The keratinocytes in polyclonal K14 mice are I-A-negative and should be incapable of activating ... Antigen presentation by keratinocytes directs autoimmune skin disease. Lian Fan, Brian W. Busser, Traci Q. Lifsted, David Lo, ... Antigen presentation by keratinocytes directs autoimmune skin disease. Lian Fan, Brian W. Busser, Traci Q. Lifsted, David Lo, ... and these keratinocytes stimulate autoreactive CD4+ cells to produce Th1 cytokines, which drive further keratinocyte ...
https://www.pnas.org/content/100/6/3386
*  Keratinocytes have the metastatic touch | Science Signaling
Sequencing of the small RNAs in melanoma cells cultured on DLL1-coated plates or cocultured with keratinocytes revealed that ... Compared with mice injected with melanoma cells and either basal keratinocytes or fibroblasts, mice injected with melanoma ... found that contact with differentiated keratinocytes in the upper epidermis triggered vertical invasion by melanoma cells. ... Noninvasive melanoma cells became invasive in Matrigel when cocultured with differentiated keratinocytes but not with basal ...
http://stke.sciencemag.org/content/8/393/ec255
*  Human Keratinocyte Cell Lines | SpringerLink
... immortalization of human epidermal keratinocytes has been generally considered to have a massive impact on their ... HaCaT Cell Human Keratinocytes Normal Keratinocytes Human Epidermal Cell Human Keratinocyte Cell Line These keywords were added ... 1991) Human Keratinocyte Cell Lines. In: Wilson G., Davis S.S., Illum L., Zweibaum A. (eds) Pharmaceutical Applications of Cell ... Fuchs E, Green H (1981) Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell 25: 617-625 ...
https://link.springer.com/chapter/10.1007/978-1-4757-0286-6_23
*  Skin cell (keratinocyte)
This normal human skin cell was treated with a growth factor that triggered the formation of specialized protein structures that enable the cell to move. We depend on cell movement for such basic functions as wound healing and launching an immune response.. Back to main page ...
https://www.nigms.nih.gov/education/life-magnified/Pages/3_bottom_skin_cell.aspx
*  Primary Epidermal Keratinocytes; Normal, Human, Adult (HEKa) ATCC ®
... when grown in Dermal Cell Basal Media supplemented with Keratinocyte Growth Kit components, provide an ideal cell system to ... propagate keratinocytes in serum-free (not animal free) conditions. The cells are cryopreserved in the first passage to ensure ... Primary Epidermal Keratinocytes; Normal, Human, Adult (HEKa) (ATCC® PCS-200-011™) Organism: Homo sapiens, human / Tissue: skin ... keratinocyte Morphology Cobblestone appearance; cells are rounded, not flat; cells display a high mitotic index; at near 80% ...
https://www.atcc.org/products/all/PCS-200-011.aspx
*  Increase developmental plasticity of human keratinocytes with gene suppression | PNAS
Increase developmental plasticity of human keratinocytes with gene suppression. Shengwen Calvin Li, Yangsun Jin, William G. ... Increase developmental plasticity of human keratinocytes with gene suppression. Shengwen Calvin Li, Yangsun Jin, William G. ... Increase developmental plasticity of human keratinocytes with gene suppression Message Subject (Your Name) has sent you a ... Increase developmental plasticity of human keratinocytes with gene suppression. Shengwen Calvin Li, Yangsun Jin, William G. ...
https://www.pnas.org/content/early/2011/07/13/1100509108
*  primary keratinocytes - HeKn - Cell Biology - BioForum
primary keratinocytes - HeKn - posted in Cell Biology: Hello! Somebody knows how cultre these cells? We are using original, ... We add 10 ng/mL KGF (from Epoch Biolabs) in keratinocyte growth medium that help a lot. But be careful, KGF from other vendors ... primary keratinocytes - HeKn. Started by bionick, Dec 10 2004 02:53 AM ...
http://www.protocol-online.org/forums/topic/4539-primary-keratinocytes-hekn/
*  Escherichia coli ghosts promote innate immune responses in human keratinocytes. - PubMed - NCBI
Escherichia coli ghosts promote innate immune responses in human keratinocytes.. Abtin A1, Kudela P, Mayr UB, Koller VJ, ... surface components for the induction of antimicrobial peptides and pro-inflammatory cytokines in human primary keratinocytes ( ...
https://www.ncbi.nlm.nih.gov/pubmed/20696136
*  Dihydroceramide Desaturase Inhibition by a Cyclopropanated Dihydroceramide Analog in Cultured Keratinocytes
... Susanne Brodesser1 ... "Dihydroceramide Desaturase Inhibition by a Cyclopropanated Dihydroceramide Analog in Cultured Keratinocytes," Journal of Lipids ...
https://www.hindawi.com/journals/jl/2011/724015/cta/
*  Keratinocytes drive melanoma invasion in a reconstructed skin model.
Keratinocytes / pathology, physiology*. Melanoma / pathology*. Models, Biological. Neoplasm Invasiveness. Neoplasm Metastasis. ... The dynamic interplay between keratinocytes and melanoma cells is further shown by an altered growth pattern of melanoma cells ... but that the interplay between surrounding keratinocytes and the melanoma cells plays an important role in melanoma invasion.. ... is secreted by the keratinocytes and subsequently activated by an unknown soluble factor secreted by the melanoma cells. ...
http://www.biomedsearch.com/nih/Keratinocytes-drive-melanoma-invasion-in/20700063.html
*  Kv7/M-type potassium channels in rat skin keratinocytes. | Sigma-Aldrich
Kv7/M-type potassium channels in rat skin keratinocytes.. [Joanne M Reilly, Vsevolod Telezhkin, Gayle M Passmore, Stephen J ... Skin keratinocytes fulfil important signalling and protective functions. Immunocytochemical experiments revealed the unexpected ... Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward ... We conclude that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, ...
https://www.sigmaaldrich.com/catalog/papers/23592175
*  Normal Human Epidermal Keratinocytes (NHEK) | PromoCell
Normal Human Epidermal Keratinocytes (NHEK). Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or ... Figure 1. Normal Human Epidermal Keratinocyte Cell Culture in phase contrast.. Figure 1. Normal Human Epidermal Keratinocyte ... Keratinocyte Growth Medium 2. Serum-free cell culture medium for keratinocytes from the epidermis. ... Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, ...
https://www.promocell.com/product/normal-human-epidermal-keratinocytes-nhek/
*  Human Keratinocytes, HEK Cell Culture - Gelatins
About 90% of epidermal cells are of the keratinocyte cell type. ... The keratinocyte is the major cell type of the epidermis, ... The keratinocyte is the major cell type of the epidermis, making up about 90% of epidermal cells. Human Epidermal Keratinocytes ... Home . Cells and Media . Human Cells & Media . Human Epidermal Keratinocytes. PrimaPure Cells. Human Epidermal Keratinocytes. * ... Human Epidermal Keratinocytes (HEK) perform a barrier function in the skin and mouth, protecting against invasion of bacteria ...
http://www.genlantis.com/human-epidermal-keratinocytes.html
*  HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. - PubMed - NCBI
HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.. Hawley-Nelson P1, Vousden KH, Hubbert NL, Lowy ... Therefore, we conclude that HPV16 E6 and E7 cooperative to immortalize human keratinocytes in vitro. Changes in cellular gene ... Co-transfection of a plasmid with an intact E6 ORF and a second plasmid with an intact E7 ORF generated keratinocyte lines with ... induced an indefinite life-span in the keratinocytes with an efficiency similar to that of the entire early region of the viral ...
https://www.ncbi.nlm.nih.gov/pubmed/2555178?dopt=Abstract
*  miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes
... Dianbao Zhang,1 Jing Wang,2 Zhe ... Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that ... β1 treatment in HaCaT cells and normal human epidermal keratinocytes (NHEK), and it was a Smad3-dependent manner. By cell ... β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes, which might represent a potential target for improving ...
https://www.hindawi.com/journals/bmri/2015/453518/abs/
*  Expression of CD90 on keratinocyte stem/progenitor cells: Ingenta Connect
To investigate the biological function of CD90+ and CD90− keratinocytes. Methods CD90+ and CD90− keratinocytes were purified ... while most of the keratinocytes maintained expression of α6 integrin. Purified CD90+ keratinocytes demonstrated a sixfold ... Here, we report the detection of CD90+ cells in cultured normal human epidermal keratinocytes and adult skin. Objectives ... The identification and purification of keratinocyte stem cells (KSCs) that are capable of self-renewal and maintenance of ...
https://www.ingentaconnect.com/content/bsc/bjd/2006/00000154/00000006/art00006
*  Ultraviolet Radiation Induction of Ornithine Decarboxylase in Rat Keratinocytes | Cancer Research
Ultraviolet Radiation Induction of Ornithine Decarboxylase in Rat Keratinocytes. Cheryl F. Rosen, Dragan Gajic and Daniel J. ... Ultraviolet Radiation Induction of Ornithine Decarboxylase in Rat Keratinocytes. Cheryl F. Rosen, Dragan Gajic and Daniel J. ... Ultraviolet Radiation Induction of Ornithine Decarboxylase in Rat Keratinocytes. Cheryl F. Rosen, Dragan Gajic and Daniel J. ... Keratinocytes grown in culture were either shamirradiated or exposed to increasing doses of UVB (1-5 mJ/cm2). Northern blot ...
http://cancerres.aacrjournals.org/content/50/9/2631
*  Keratinocyte-Derived Cytokine
... Detection. Mouse keratinocyte-derived cytokine (KC) is the homolog of the human chemokine (C-X-C ... ELISAs for Keratinocyte-Derived Cytokine Detection. The Mouse GRO/KC Assay Kit (Cat. # 27137A) is a solid-phase sandwich ELISA ... Antibodies for Keratinocyte-Derived Cytokine Detection. KC (CXCL1) antibody products (Cat. # 18367A, B; 18361A, B; and 18366A) ... Keratinocyte-derived cytokine was previously called growth-regulated alpha protein or GRO-alpha; alternative names include ...
http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Cytokines/Keratinocyte-Derived_Cytokine