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*  AID 673641 - Binding affinity to UBA1 in human Jurkat cells at 150 uM followed by UV irradiation for 30 mins with Wood's glass...
... at 150 uM followed by UV irradiation for 30 mins with Wood's glass filtered medium pressure mercury arc light prior to cell ... Binding affinity to UBA1 in human Jurkat cells ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/673641
*  Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic...
... in acute lymphoblastic leukemia Jurkat cells and to characterize the underlying ... Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic ... Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic ... PWGPE induced apoptosis in Jurkat cells in a time- and concentration-dependent manner. The anticancer effect was associated ...
http://pubs.rsc.org/en/content/articlelanding/2017/fo/c7fo00263g
*  Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line
... Macho, A. ; Blanco-Molina, M. ; ... Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. De Montfort University Open Research ... Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. Biochemical Pharmacology, 68 (5), pp. ...
https://www.dora.dmu.ac.uk/handle/2086/2858
*  Estrogen Receptor-Mediated, Nitric Oxide-Dependent Modulation of the Immunologic Barrier Function of the Endothelium |...
A, RT-PCR on RNA from PMA/ionomycin-activated Jurkat cells (lane 1), nonactivated Jurkat cells (lane 2), HUVECs (lane 3), and ... resulted in changes in the cell-surface expression of Fas in Jurkat cells, a portion of the cells were centrifuged and ... Cells were washed twice with PBS and coincubated with 105 Jurkat cells in M199 plus 2% defined (,10−10 mol/L E2) FBS in the ... Cytospin cell preparations of the Jurkat cells were fixed in 4% paraformaldehyde at room temperature for 1 hour to perform ...
http://circ.ahajournals.org/content/104/21/2576
*  ScienceAsia - Journal of The Science Society of Thailand
... were investigated to assess the down-regulation of Nck1 protein efficiency in the Jurkat T cell line. Jurkat T cells treated ... RNAi down-regulation of Nck1 adaptor protein in Jurkat T cells. Pussadee Paensuwana, Jatuporn Ngoenkama, Donruedee ... However, these T cell activation outcomes were increased in lentiviral vector based Nck1-shRNA transfected cells. These data ... CD69 expression and IL-2 production were impaired in Nck1-siRNA and plasmid-based Nck1-shRNA transfected Jurkat cells. ...
http://www.scienceasia.org/content/viewabstract.php?ms=5201&v=32&abst=1
*  Neo Jurkat ATCC ® CRL-2898™ Homo sapiens peripheral blood Ac
Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg ... The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector ... The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector ... The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector ...
https://www.atcc.org/en/Products/All/CRL-2898.aspx?p=1&rel=%7B0%7D
*  Dead or Inflamed: How to differentiate apoptotic from necroptotic cell death? Part II.
... are not susceptible to necroptotic cell death. Interestingly, in contrast to jurkat cells, U937 cells also undergo necroptosis ... This suggests that TRAIL may signal differently in U937 cells than it does in jurkat cells. I don't know why this is, but I'll ... The most commonly used model cell line for necroptosis are the murine fibroblast cells L929. These cells are extremely ... suggesting that caspase-8 is not essential to prevent necroptosis in these cells. The RIPK1-deficient jurkat cell line is not ...
http://deadorinflamed.blogspot.co.uk/2014/04/how-to-differentiate-apoptotic-from_5.html
*  data have suggested that activation of the inducible T-cell kinase (ITK) - NF-κB signaling in inflammation and cancer
MATERIALS AND METHODS Cell lines mice antibodies and additional reagents. Wild-type Jurkat T cells (E6.1) were from the ... Here we show that this cell-permeable peptide henceforth called R9-QQP is readily taken up by both Jurkat T cells and murine ... Therefore association of ITK and SLP-76 recruitment of ITK and actin polarization in the T-cell contact site LCK-mediated ... data have suggested that activation of the inducible T-cell kinase (ITK) requires an connection with the adaptor protein SLP-76 ...
http://healthcarecoremeasures.com/2017/01/08/data-have-suggested-that-activation-of-the-inducible-t-cell-kinase-itk/
*  Electroporation condition for Jurkat cells
... Venkat Pisupati vnp at mole.bio.cam.ac.uk Mon Sep 2 07:54:04 EST 1996 *Previous ... hi! is anyone out there tried electroporating Jurkat cells? If so, can you post the electroporation conditions please. cheers ...
http://www.bio.net/bionet/mm/methods/1996-September/048620.html
*  jurkat Cells | Thermo Fisher Scientific - CA
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ...
https://www.thermofisher.com/ca/en/home/technical-resources/cell-lines/j/cell-lines-detail-7.html
*  Jurkat cell - Tissue and Cell Culture - BioForum
... posted in Tissue and Cell Culture: Hi How can I do to separate live and dead Jurkat cells.? I recently thawed this cell line. I ... counted with trypan blue and saw many dead cells. Please, help me !!! I do not want to lose these cells. ... LPS-stimulation of Jurkat T-cells to induce TNF-alpha Started by TZ89, 11 Jun 2013 LPS, Jurkat, TNF, T-cell * 0 replies ... Stimulating Jurkat T Cells with PMA: Clumping Cells? Started by chuggleschuggles, 23 Oct 2014 jurkat, stimulation, pma and 1 ...
http://www.protocol-online.org/forums/topic/32134-jurkat-cell/
*  Potentiation of bleomycin in Jurkat cells by fungal pycnidione. | Sigma-Aldrich
Potentiation of bleomycin in Jurkat cells by fungal pycnidione.. [Mayumi Kaneko, Daisuke Matsuda, Masaki Ohtawa, Takashi Fukuda ... Pycnidione irreversibly abrogated bleomycin-induced G2 arrest in Jurkat cells and synergically potentiated the cytotoxicity of ... Most cancer cells have mutations in genes at the G1 checkpoint and repair DNA only in the G2 phase; therefore, the G2 ... indicating that cells did not arrest at the G2 phase. Moreover, under these conditions, Chk1 and Chk2 levels were markedly down ...
https://www.sigmaaldrich.com/catalog/papers/22223332
*  Jurkat cells - Wikipedia
Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell ... Jurkat Cells at the US National Library of Medicine Medical Subject Headings (MeSH) Cellosaurus entry for Jurkat. ... Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell ... The I 9.2 and I 2.1 cell lines. The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally ...
https://en.wikipedia.org/wiki/Jurkat_cells
*  HNRNPD antibody (ARP40238 T100) | Tested with: Human Jurkat cells
HNRNPD is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells , Alias: P37; AUF1; AUF1A; ... 293/Jurkat/3T3/COLO205 cells. HNRNPD Antibody (OAAF01114). Application: WB, IHC, IF, ELISA. Species: Human, Mouse. ... Product Protocols: HNRPD antibody tested with Human Jurkat Cells (ARP40238_T100). Product Protocols: HNRPD antibody tested by ... and GRHL2 in human oral squamous cell carcinoma cells. Oncogene 28, 565-74 (2009). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human ...
https://www.avivasysbio.com/hnrpd-antibody-c-terminal-region-arp40238-t100.html
*  Incorporation studies of clickable ceramides in Jurkat cell plasma membranes - Chemical Communications (RSC Publishing)
The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues ... Incorporation studies of clickable ceramides in Jurkat cell plasma membranes T. Walter, J. Schlegel, A. Burgert, A. Kurz, J. ... The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues ...
http://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C7CC01220A
*  Comparison of Jurkat cell rolling velocities on various | Open-i
Jurkat cells (2 × 106 cells/ml) were tethered onto different GlyCAM-1/IgG chimer ... Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. ... Figure 3: Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. Jurkat cells (2 × 106 cells/ml) were ... Figure 3: Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. Jurkat cells (2 × 106 cells/ml) were ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2195654_JEM982276.f3&req=4
*  Inhibitory effect of Embelin on human acute T cell lymphoma Jurkat cells through activation of the apoptotic pathway. - PubMed...
The present study examined the effects of Embelin on the proliferation of human acute T cell lymphoma Jurkat cells. Jurkat ... Effect of Embelin on the proliferation of Jurkat cells. Viability represents the percentage of viable Jurkat cells measured ... Inhibitory effect of Embelin on human acute T cell lymphoma Jurkat cells through activation of the apoptotic pathway.. Zhu XL1 ... The results showed that Embelin significantly inhibited the growth of human acute T cell lymphoma Jurkat cells. Following ...
https://www.ncbi.nlm.nih.gov/pubmed/26622596
*  Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells.
... Samali A. ... In the present study, we demonstrate that pro-caspase-3 is present in the mitochondrial fraction of Jurkat T cells in a complex ... finding suggests that the release of mitochondrial Hsps may also accelerate caspase activation in the cytoplasm of intact cells ...
https://www.uniprot.org/citations/10205158
*  Distinct Pathways of Apoptosis Triggered by FTY720, Etoposide, and Anti-Fas Antibody in Human T-Lymphoma Cell Line (Jurkat...
Prevention of cleavages of caspases, Bid, and PARP in Jurkat-Bcl-2 cells. Jurkat-Neo cells and Jurkat-Bcl-2 cells were ... Jurkat-Neo cells and Jurkat-Bcl-2 cells were incubated for 4 h in the presence of 10 μM FTY720, 50 μg/ml etoposide, and 1 μg/ml ... Cell Culture and Induction of Apoptosis.. The Jurkat-Bcl-2 cell line, human T-lymphoma cells stably transfected with a human ... Nuclear fragmentation of Jurkat cells treated with FTY720, etoposide, and anti-Fas antibody. Jurkat-Neo cells were incubated ...
http://jpet.aspetjournals.org/content/300/3/939
*  AID 298664 - Induction of apoptosis in Jurkat cells assessed as cell viability at 50 uM after 48 hrs by flow cytometry Annexin...
Induction of apoptosis in Jurkat cells assessed as cell viability at 50 uM after 48 hrs by flow cytometry Annexin-V/propidium ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/298664
*  Expression of a functional CD3-Ti antigen/MHC receptor in the absence of surface CD2. Analysis with clonal Jurkat cell mutants....
Analysis with clonal Jurkat cell mutants.. P Moingeon, A Alcover, L K Clayton, H C Chang, C Transy, E L Reinherz ... Assuming that the Jurkat cell line is representative of normal cycling human T lymphocytes, we conclude that the presence of ... Jurkat variants. These mutants lack detectable surface CD2 as determined by indirect immunofluorescence, immunoprecipitation ...
http://jem.rupress.org/content/168/6/2077
*  l-Arginine Regulates the Expression of the T-Cell Receptor ζ Chain (CD3ζ) in Jurkat Cells | Clinical Cancer Research
l-Arginine Regulates the Expression of the T-Cell Receptor ζ Chain (CD3ζ) in Jurkat Cells. Faramaz Taheri, Juan B. Ochoa, Zahra ... l-Arginine Regulates the Expression of the T-Cell Receptor ζ Chain (CD3ζ) in Jurkat Cells ... l-Arginine Regulates the Expression of the T-Cell Receptor ζ Chain (CD3ζ) in Jurkat Cells ... l-Arginine Regulates the Expression of the T-Cell Receptor ζ Chain (CD3ζ) in Jurkat Cells ...
http://clincancerres.aacrjournals.org/content/7/3/958s
*  Phorbol 12-Myristate 13-Acetate Protects Jurkat Cells from Methylglyoxal-Induced Apoptosis by Preventing c-Jun N-Terminal...
We next assessed the levels of apoptosis by stimulating three cell lines, Jurkat cells, Ras WT cells, and Ras N17 cells, with ... Cell Lines and Culture Conditions. Jurkat human T-cell leukemia cells were cultured in RPMI 1640 medium supplemented with 10% ... PMA inhibits MG-induced apoptosis in Jurkat cells. A, Jurkat cells were preincubated with or without 50 ng/ml PMA for 1 h and ... The MEK/ERK pathway modulates caspase activation in MG-treated Jurkat cells. A, Jurkat cells were preincubated with or without ...
http://molpharm.aspetjournals.org/content/65/3/778
*  Oxidation of pyridine nucleotides during Fas- and ceramide-induced apoptosis in Jurkat cells: correlation with changes in...
Oxidation of pyridine nucleotides during Fas- and ceramide-induced apoptosis in Jurkat cells: correlation with changes in ... Oxidation of pyridine nucleotides during Fas- and ceramide-induced apoptosis in Jurkat cells: correlation with changes in ... Oxidation of pyridine nucleotides during Fas- and ceramide-induced apoptosis in Jurkat cells: correlation with changes in ... Oxidation of pyridine nucleotides during Fas- and ceramide-induced apoptosis in Jurkat cells: correlation with changes in ...
http://www.biochemj.org/content/354/3/727.3
*  CD27 Nf-kB Jurkat Cell Line
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NF-kB response elements with constitutive ... CD27 is expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-κB and MAPK/JNK ... Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NF-kB response elements with constitutive ... Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license ...
http://bpsbioscience.com/immunotherapy/cell-based-assays-stable-lines/cd27-nf-kb-reporter-jurkat-recombinant-cell-line-79509