Lirias: PepX: a structural database of non-redundant protein-peptide complexes
Although protein-peptide interactions are estimated to constitute up to 40% of all protein interactions, relatively little information is available for the structural details of these interactions. Peptide-mediated interactions are a prime target for drug design because they are predominantly present in signaling and regulatory networks. A reliable data set of nonredundant protein-peptide complexes is indispensable as a basis for modeling and design, but current data sets for protein-peptide interactions are often biased towards specific types of interactions or are limited to interactions with small ligands. In PepX (http://pepx.switchlab.org), we have designed an unbiased and exhaustive data set of all protein-peptide complexes available in the Protein Data Bank with peptide lengths up to 35 residues. In addition, these complexes have been clustered based on their binding interfaces rather than sequence homology, providing a set of structurally diverse protein-peptide interactions. The ...https://lirias.kuleuven.be/handle/123456789/292339
Mitogenic effects of hepatic stimulator substance on cultured nonparenchymal liver epithelial cells - Gupta - 2005 - Hepatology...
Gupta, S., LaBrecque, D. R. and Shafritz, D. A. (1992), Mitogenic effects of hepatic stimulator substance on cultured nonparenchymal liver epithelial cells. Hepatology, 15: 485-491. doi: 10.1002/hep.1840150322 ...http://onlinelibrary.wiley.com/doi/10.1002/hep.1840150322/references
Cytomegalovirus (CMV) Control Peptide Pool
Peptides , CEF Control Peptides , Cytomegalovirus (CMV) Control Peptide Pool; These 5 Cytomegalovirus (CMV) peptides, at 0.25 mg each (total of 1.25 mg/vial), constitute part of the CEF control peptide pool (cat# 61036-025). Now available separately as CMV control peptide pool, Influenza control peptide pool (cat# 62340) and EBV control peptide pool (cat# 62341), these peptides have been used in the stimulation of IFNgamma release from CD8+ T cells in individuals with defined HLA types, these epitopes are useful in applications such as ELISPOT, intracellular cytokine and CTL assays. All peptides are provided as net weight based on peptide content.https://www.anaspec.com/products/product.asp?id=44991
Nanofiber-based delivery of therapeutic peptides to the brain. | nanomedicines.org
The delivery of therapeutic peptides and proteins to the central nervous system is the biggest challenge when developing effective neuropharmaceuticals. The central issue is that the blood-brain barrier is impermeable to most molecules. Here we demonstrate the concept of employing an amphiphilic derivative of a peptide to deliver the peptide into the brain. The key to success is that the amphiphilic peptide should by design self-assemble into nanofibers wherein the active peptide epitope is tightly wrapped around the nanofiber core. The nanofiber form appears to protect the amphiphilic peptide from degradation while in the plasma, and the amphiphilic nature of the peptide promotes its transport across the blood-brain barrier. Therapeutic brain levels of the amphiphilic peptide are achieved with this strategy, compared with the absence of detectable peptide in the brain and the consequent lack of a therapeutic response when the underivatized peptide is administered.. ...http://www.nanomedicines.org/node/14
nanoHUB.org - Resources: KIST/PU Phage Display Selection of Tissue-Specific Homing Peptides and their Theragnostic Applications...
Several homing peptides that are specific to tumor cells, artherosclerotic plaques, stroke lesion, apoptotic cells and phosphatidylserine have been isolated by phage display in our laboratory. For example, the Bld-1 peptide is specific to bladder tumor cells and the AP-1 to atherosclerotic plaques. These peptides could be used in targeted delivery either alone or in combination with nanoparticles. We have demonstrated that the Bld-1 peptide can be homing to bladder tumor in vivo and showed a tumor specific PET imaging in rat and mouse models. Moreover, we assembled the Bld-1 peptide with hydrophobically-modified glycol chitosan (HGC) nanoparticles for carrying an anticancer drug and a nearinfrared optical imaging dye for targeted delivery and imaging simultaneously. The AP-1 peptide alone or assembled with HGC nanoparticles could home to atherosclerotic plaques in LDL receptor-deficient mice. These results demonstrate the potential of the homing peptide technology for ...http://nanohub.org/resources/3375/play
Mimicking proteasomal release of polyglutamine peptides initiates aggregation and toxicity | Journal of Cell Science
Based on our findings, we propose a model in which expanded polyQ peptides are resistant to degradation, and their accumulation leads to intracellular polyQ aggregates (Fig. 5). Proteasomes are rapidly recruited into the polyQ core, possibly in a final attempt to degrade the expanded polyQ peptides. Subsequently, other proteins are sequestered and ubiquitylated, perhaps because of (partial) unfolding. These events also lead to the binding of chaperones such as Hsp70 that may recognize denatured proteins. All these events result in concentric ring-like structures formed around the aggregate (Fig. 5). Essential proteins are depleted from the cell, contributing to cellular dysfunction. We conclude that polyQ peptides may be fundamental in initiating aggregation and sequestration of different types of proteins including polyQ proteins. Although FRAP experiments ...http://jcs.biologists.org/content/122/18/3262
EP 1064010 A1 20010103 - PEPTIDE COMPOSITIONS AND FORMULATIONS AND USE OF SAME
294545266 - EP 1064010 A1 2001-01-03 - PEPTIDE COMPOSITIONS AND FORMULATIONS AND USE OF SAME - [origin: WO9945941A1] The present invention is directed to a composition which is used to enhance the softness, elasticity, or appearance of tissue. Specifically, the present invention is directed to a composition formulated from peptides which substantially correspond to those produced from thermolysing digestion of elastin. This formulation is preferably applied to human skin in a cosmetic or therapeutic formulation. The present composition specifically includes the known chemical modification of the peptides described herein, specifically carboxy and amino modification including the addition of amino acids to either end of the peptide fragments.[origin: WO9945941A1] The present invention is directed to a composition which is used to enhance the softness, elasticity, or appearance of tissue. Specifically, the present invention is directed to a composition formulated from ...https://data.epo.org/gpi/EP1064010A1-PEPTIDE-COMPOSITIONS-AND-FORMULATIONS-AND-USE-OF-SAME
DSpace@MIT: A self-assembling peptide scaffold functionalized for use with neural stem cells
The performance of a biological scaffold formed by the self-assembling peptide RADA16 is comparable to the most commonly used synthetic materials employed in the culture of neural stem cells. Furthermore, improvements in the performance of RADA16 have recently been made by appending the self-assembling peptide sequence with various functional motifs from naturally occurring proteins. The focus of this work is to further analyze the performance of these functionalized self-assembling peptide scaffolds when used for the culture of neural stem cells, and to characterize these newly developed materials for comparison with RADA16. The effect of the functional motifs on the structure of the peptide scaffold was evaluated with circular dichroism and scanning electron microscopy, and the mechanical properties of the peptide scaffolds were examined through theological analysis. The functionalized peptides were found to have lower percentages of beta-sheet structure as well as reduced ...http://dspace.mit.edu/handle/1721.1/33396
Viruses | Free Full-Text | How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to...
Immune monitoring of T cell responses increasingly relies on the use of peptide pools. Peptides, when restricted by the same HLA allele, and presented from within the same peptide pool, can compete for HLA binding sites. What impact such competition has on functional T cell stimulation, however, is not clear. Using a model peptide pool that is comprised of 32 well-defined viral epitopes from Cytomegalovirus, Epstein-Barr virus, and Influenza viruses (CEF peptide pool), we assessed peptide competition in PBMC from 42 human subjects. The magnitude of the peptide pool-elicited CD8 T cell responses was a mean 79% and a median 77% of the sum of the CD8 T cell responses elicited by the individual peptides. Therefore, while the effect of peptide competition was evident, it was of a relatively minor magnitude. By studying the dose-response curves for individual CEF peptides, we show that several of these peptides are present in the CEF-pool at ...http://mdpi.com/1999-4915/4/11/2636/xml
Viruses | Free Full-Text | How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to...
Immune monitoring of T cell responses increasingly relies on the use of peptide pools. Peptides, when restricted by the same HLA allele, and presented from within the same peptide pool, can compete for HLA binding sites. What impact such competition has on functional T cell stimulation, however, is not clear. Using a model peptide pool that is comprised of 32 well-defined viral epitopes from Cytomegalovirus, Epstein-Barr virus, and Influenza viruses (CEF peptide pool), we assessed peptide competition in PBMC from 42 human subjects. The magnitude of the peptide pool-elicited CD8 T cell responses was a mean 79% and a median 77% of the sum of the CD8 T cell responses elicited by the individual peptides. Therefore, while the effect of peptide competition was evident, it was of a relatively minor magnitude. By studying the dose-response curves for individual CEF peptides, we show that several of these peptides are present in the CEF-pool at ...http://www.mdpi.com/1999-4915/4/11/2636/htm
Custom Peptide Synthesis
Cancer immunotherapy: Cancer immunotherapy uses the body's own immune system to attack cancer cells. Peptide-based vaccines use tumor-associated antigens that associate with T cells to target cancer.. Self-assembling peptide: Self-assembling peptides are short, synthetic peptides characterized by amphipathic sequences. These peptides are able to spontaneously self-assemble in aqueous solution to form highly organized structures such as hydrogels. APExBIO can synthesize hydrophobic self-assembling sequences at high quantity and purity.. Peptide Venom Peptides: Bioactive peptides are the most dominant component of animal venoms. Venom peptides can vary in length and complexity, thus their synthesis requires a combination of chemical and recombinant synthesis.. Peptide Natriuretic Peptides: Functioning in the induction of natriuresis, (the excretion of large amounts of sodium in the urine), ...https://www.apexbt.com/custom-peptide-synthesis
Faculty Collaboration Database - DeNovoID: a web-based tool for identifying peptides from sequence and mass tags deduced from...
One of the core activities of high-throughput proteomics is the identification of peptides from mass spectra. Some peptides can be identified using spectral matching programs like Sequest or Mascot, but many spectra do not produce high quality database matches. De novo peptide sequencing is an approach to determine partial peptide sequences for some of the unidentified spectra. A drawback of de novo peptide sequencing is that it produces a series of ordered and disordered sequence tags and mass tags rather than a complete, non-degenerate peptide amino acid sequence. This incomplete data is difficult to use in conventional search programs such as BLAST or FASTA. DeNovoID is a program that has been specifically designed to use degenerate amino acid sequence and mass data derived from MS experiments to search a peptide database. Since the algorithm employed depends on the amino acid composition of the peptide and not its sequence, DeNovoID does not have to consider all possible ...https://fcd.mcw.edu/?module=search&func=showPublication&id=42282
China Peptide Supply Peptide Product Ghrp6, Top Grade Ghrp6 - Buy Peptide Hormone, CAS 87616-84-0 - China Peptides,...
China Peptide Supply Peptide Product Ghrp6, Top Grade Ghrp6 - Buy Peptide Hormone, CAS 87616-84-0, Find details about China Peptides, Pharmaceutical Intermediate from Peptide Supply Peptide Product Ghrp6, Top Grade Ghrp6 - Buy Peptide Hormone, CAS 87616-84-0 - Zhengzhou Filter Biotechnology Co., Ltd.http://filter-biotechnology.en.made-in-china.com/product/SvhEzJfMVTrP/China-Peptide-Supply-Peptide-Product-Ghrp6-Top-Grade-Ghrp6-Buy-Peptide-Hormone-CAS-87616-84-0.html
Enzymatic transhalogenation of dendritic RGD peptide constructs with the fluorinase
The substrate scope of fluorinase enzyme mediated transhalogenation reactions is extended. Substrate tolerance allows a peptide cargo to be tethered to a 5'-chloro-5'-deoxynucleoside substrate for transhalogenation by the enzyme to a 5'-fluoro-5'-deoxynucleoside. The reaction is successfully extended from that previously reported for a monomeric cyclic peptide (cRGD) to cargoes of dendritic scaffolds carrying two and four cyclic peptide motifs. The RGD peptide sequence is known to bind upregulated αVβ3 integrin motifs on the surface of cancer cells and it is demonstrated that the fluorinated products have a higher affinity to αVβ3 integrin than their monomeric counterparts. Extending the strategy to radiolabelling of the peptide cargoes by tagging the peptides with [18F]fluoride was only moderately successful due to the poor water solubility of these higher order peptide scaffolds although the strategy holds promise for peptide constructs with improved solubility ...https://research-repository.st-andrews.ac.uk/handle/10023/10303
Critical self-assembly concentration of bolaamphiphilic peptides and peptide hybrids determined by fluorescence measurements
MARTARI, Marco and SANDERSON, Ronald D.. Critical self-assembly concentration of bolaamphiphilic peptides and peptide hybrids determined by fluorescence measurements. S.Afr.j.chem. (Online) [online]. 2008, vol.61, pp.47-52. ISSN 1996-840X.. The study of the self-assembly properties of peptides and proteins is important for the understanding of molecular recognition processes and for the rational design of functionalbiomaterials. Novel bolaamphiphilic peptides and peptide hybrids incorporating non-natural aminoacids were designed around a model lysine/leucine-rich peptide with the intention to study their self-assembly behaviour. Steady-state fluorescence measurements using pyrene as fluorescent probe were adapted to the determination of the critical self-assembly concentrations (CSACs) of these amphiphilic peptides and peptide hybrids. Different experimental conditions were studied. The morphology of the peptide aggregates ...http://www.scielo.org.za/scielo.php?script=sci_abstract&pid=S0379-43502008000100010&lng=en&nrm=iso&tlng=en
Modelling, NMR and synthesis of food peptides. - Surrey Research Insight Open Access
The work in this thesis can be divided into two sections, namely the study of delicious peptide, a food flavour and the antimicrobial peptide lactofenicin B. The main interest in these compounds is in terms of structure and conformation adopted in solution and how this relates to their mode of action. Delicious peptide was studied initially by 1H NMR spectroscopy for evidence of a specific solution structure. Results show that delicious peptide does not adopt a regular conformation in solution. Molecular dynamics simulations of this peptide show the flexibility of the peptide structure in solution. Quenched molecular dynamics simulations were used to search for low energy conformers of the peptide. The results suggest that the flavour of the peptide is produced by interaction of basic and acidic regions in the peptide. The work was extended to examine delicious peptide analogues with similar flavour characteristics. The results obtained suggest that similar interactions of basic and acidic ...http://epubs.surrey.ac.uk/842985/
Strategies to deliver peptide drugs to the brain. | nanomedicines.org
Neurological diseases such as neurodegeneration, pain, psychiatric disorders, stroke, and brain cancers would greatly benefit from the use of highly potent and specific peptide pharmaceuticals. Peptides are especially desirable because of their low inherent toxicity. The presence of the blood brain barrier (BBB), their short duration of action, and their need for parenteral administration limits their clinical use. However, over the past decade there have been significant advances in delivering peptides to the central nervous system. Angiopep peptides developed by Angiochem (Montreal, Canada), transferrin antibodies developed by ArmaGen (Santa Monica, USA), and cell penetrating peptides have all shown promise in delivering therapeutic peptides across the BBB after intravenous administration. Noninvasive methods of delivering peptides to the brain include the use of chitosan amphiphile nanoparticles for oral ...http://www.nanomedicines.org/node/7
Structural homologies between two HLA B27-restricted peptides suggest residues important for interaction with HLA B27. -...
Recently we described an HLA B27-restricted peptide derived from HIV gag p24 protein. In this study we have isolated an HLA B27-restricted peptide from the nucleoprotein (NP) of influenza A virus. The shortest fragment recognized by cytotoxic T lymphocyte (CTL) is eight amino acids long, residues 384-391. Comparison of the sequence of these two HLA B27 restricted peptides reveals homologies which can be aligned from one peptide to the other. Of the eight residues, two are identical: tryptophan and isoleucine. Both peptides have a positively charged residue at the N terminus, lysine at position 265 of gag and arginine at position 384 of NP. Using modified peptides we have shown that lysine or arginine is crucial for the interaction with HLA B27. The wild-type gag peptide blocked CTL recognition of NP peptide by influenza-specific CTL, but removal of the lysine prevented inhibition of NP peptide recognition. The importance of these charged residues was ...https://www.rdm.ox.ac.uk/publications/35090
Indian Patents. 240957:'A PEPTIDE THAT IS A MATURATION PRODUCT OF THE BASIC PROLIN-RICH LACRIMAL PROTEIN (BPLP)OR A PEPTIDE...
The invention relates to a peptide that is a maturation product of the Basic Prolin-rich Lacrinal Protein (BPLP) or a peptide derivative or a mimetic of said maturation product, wherein the peptide or peptide derivative or mimetic exhibits an inhibitory property against a metallo-ectopeptidase, especially NEP and/or APN. The present invention also relates to polynucleotides coding for said peptides and to antibodies directed against said peptides. Furthermore, the present invention relates to diagnostic and therapeutic uses of human BPLP protein and inhibitory peptides derived therefrom, polypeptides coding for human BPLP protein or peptides derived therefrom as well as antibodies directed against BPLP protein or peptides derived therefrom ...http://www.allindianpatents.com/patents/240957-a-peptide-that-is-a-maturation-product-of-the-basic-prolin-rich-lacrimal-protein-bplp-or-a-peptide-derivatives-thereof
Peter Thomas Roth's Deal with Serum: Benefits of Peptides on your Skin - Agosuper
Pores and skin professionals recommend facial products that have peptides (like Peter Thomas Roth Un-wrinkle Turbo Experience Serum) to be a an effective, protected way to realize radiant pores and serum for oily skin. Please read on to master precisely what a peptide is, and why several types of peptides are adept at reviving pores and skin. You may also get some simple info on why Peter Thomas Roth peptide serum is so productive at bettering pores and skin.. Introduction to Peptides:. A peptide is usually a string of amino acids, the basic elements are used to make proteins. As handful of as two amino acids can appear together to sort a peptide (exclusively a dipeptide). Other peptides contain dozens of amino acids. Generally, fifty amino acids is the cut-off line - anymore than this, and you're considering a protein, not a peptide.. The Significant Job Peptides Perform in Pores and skin Health and ...http://www.agosuper.com/peter-thomas-roths-deal-with-serum-benefits-of-peptides-on-your-skin/
How to Tie Peptide Knots | UH Department of Chemical and Biomolecular Engineering
With the advent of high throughput biomolecular engineering techniques, it has become possible to isolate short peptides that bind to a variety of targets ranging from inorganic materials to proteins. The application of peptides as therapeutics has been hampered by the inherent susceptibility of peptides to proteases present throughout the human body. One strategy to overcome this protease susceptibility is to fortify peptides via cyclization or other conformational constraints. Indeed, nature uses this strategy in several classes of peptides such as cyclotides and defensins which are stabilized by networks of disulfide bonds and in some cases head-to-tail cyclization. My group studies a class of peptides termed lasso peptides because of their unique slipknot-like structure. This highly entropically disfavored fold endows the peptides with tremendous ...http://chbe.egr.uh.edu/research/seminar/201203/how-tie-peptide-knots
Patent US5882645 - Peptide compounds - Google Patents
Synthetic peptides are widely used to generate antibodies. To induce high antibody response, it is known to conjugate the peptide to a carrier protein (e.g. KLH, BSA) or to incorporate it into polylysine to form a multiple antigenic peptide. Anchors may be built in which are based on fatty acids. According to the invention there is provided a novel lipidic amino acid based anchor system which can maximally enhance the antigenicity of a short synthetic peptide. These novel compounds are entirely peptide-based and may therefore be produced automatically by some step wise peptide synthesis, preferably solid phase step wise peptide synthesis. According to the invention there is also provided such a process.http://www.google.com/patents/US5882645?dq=4168396
Peptides for the Treatment of Cancer Associated with the Human Papilloma Virus (HPV) and Other Epithelial Tumors - Patent...
68111PRTArtificial SequenceSynthetic Peptide 1Cys Ser Val Arg Gln Gly Pro Val Gln Lys Cys1 5 10211PRTArtificial SequenceSynthetic Peptide 2Cys Ser Ser Cys Gln Asn Ser Pro Ala Leu Cys1 5 10311PRTArtificial SequenceSynthetic Peptide 3Cys Gln Ile Pro Gln Arg Thr Ala Thr Arg Cys1 5 10411PRTArtificial SequenceSynthetic Peptide 4Cys Ala Lys Gln Arg Thr Asp Pro Gly Tyr Cys1 5 10511PRTArtificial SequenceSynthetic Peptide 5Cys Trp Met Ser Pro Arg His Leu Gly Thr Cys1 5 10611PRTArtificial SequenceSynthetic Peptide 6Cys Arg Asn Cys Thr Val Ile Gln Phe Ser Cys1 5 10711PRTArtificial SequenceSynthetic Peptide 7Cys His Tyr Ile Ala Gly Thr Val Gln Gly Cys1 5 10811PRTArtificial SequenceSynthetic Peptide 8Cys Pro Leu Val Ser Leu Arg Asp His Ser Cys1 5 10911PRTArtificial SequenceSynthetic Peptide 9Cys Lys Gln Ser Tyr Leu His His Leu Leu Cys1 5 101011PRTArtificial SequenceSynthetic Peptide 10Cys Phe Gln Pro Leu Thr Pro Leu Cys Arg Cys1 5 101111PRTArtificial SequenceSynthetic Peptide 11Cys Gln Ser Tyr His Glu Leu ...http://www.patentsencyclopedia.com/app/20090005294
Services: Custom Peptide Synthesis Services at BioConcept?
High Quality Peptide for Industry and Research Applications BioConcept has a team of expert professionals using solid phase and solution phase chemistries by employing Fmoc and t-Boc methodologies for the design and production of peptides for research purposes. It?s the best place for you to outsource your routine peptide needs. BioConcept offers a range of peptide services from small research scale to large scale synthesis. Hallmarks of our custom services: Both small and large scale peptide synthesis ranging from milligram to multigram. Peptides purity range to suit customer requirement. Peptides available with various modifications. Peptides are synthesized by Fmoc chemistry on the CS Bio automated multiple peptide synthesizer. Free personalized non-obligatory technical consultation. Currently the shipping is at no extra charge within India. Complete customer confidentiality. Fast, on time delivery. ...http://bioconceptlabs.com/article_print.php?id=12
Frontiers | Peptide and Peptide-Dependent Motions in MHC Proteins: Immunological Implications and Biophysical Underpinnings |...
Structural biology of peptides presented by class I and class II MHC proteins has transformed immunology, impacting our understanding of fundamental immune mechanisms and allowing researchers to rationalize immunogenicity and design novel vaccines. However, proteins are not static structures as often inferred from crystallographic structures. Their components move and breathe individually and collectively over a range of timescales. Peptides bound within MHC peptide binding grooves are no exception, and their motions have been shown to impact recognition by TCRs and other receptors in ways that influence function. Further, peptides tune the motions of MHC proteins themselves, which impacts recognition of peptide/MHC complexes by other proteins. Here we review the motional properties of peptides in MHC binding grooves and discuss how peptide properties can influence MHC ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00935/full