Cells exhibited membrane blebbing and increased intracellular spacing in response to 100 ng/mL (5 nmol/L) BFT, which was deemed ... Notch receptors are membrane-bound receptors with a cytoplasmic region or Notch intracellular domain (NICD). NICD translocates ... increased intracellular separation, membrane blebbing, and an increase in F-actin stress fibers, whereas the control cells ... Immunofluorescence analysis with an antibody specific for the intracellular domain of E-cadherin showed a loss of membrane- ...

This work expands the potential roles of cell-cell fusogens to intracellular membrane trafficking and suggests a new way to ... These types of membrane merging events initiate on the exoplasmic (non-cytosolic) side of a cells plasma membrane, and fuse ... Within an individual cell, some membrane trafficking events, including endocytic scission, also involve membrane merging that ... Cell-cell fusogens of the Fusexin structural family mediate membrane merging during viral infection, fertilization, and ...

Genes relevant for vesicle trafficking-including those coding for Rab, which participates in intracellular membrane trafficking ... Intra-cellular storage, transport and exocytosis of halogenated compounds in marine red alga Laurencia obtusa. J Struct Biol162 ... Intracellular signaling mediated by a MAPK cascade, small GTPases, phosphatidylinositol, and calcium calmodulin-dependent ... The MAPK cascade transduces extracellular stimuli into intracellular responses during plant defense against pathogens and can ...

The DDHD domain function is not currently known but it may be implicated in phospholipid metabolism, membrane turnover, or ... Activation ofPYK2 by agents that elevate intracellular calcium or by phorbol ester inducetyrosine phosphorylation of Nirs. ... intracellular calcium, and stresssignals. Here we describe the molecular cloning and characterization of a novelfamily of PYK2- ...

The DDHD domain function is not currently known but it may be implicated in phospholipid metabolism, membrane turnover, or ... Activation ofPYK2 by agents that elevate intracellular calcium or by phorbol ester inducetyrosine phosphorylation of Nirs. ... intracellular calcium, and stresssignals. Here we describe the molecular cloning and characterization of a novelfamily of PYK2- ...

Rapid changes in electrophysiology and intracellular calcium provide evidence for a membrane-bound progesterone receptor in ... This antibody was raised to a membrane-bound antigen involved in calcium influx subsequent to progesterone binding in sperm. ... thus we suggest that progesterone acts upon many aspects of TS-TEC physiology through both cytoplasmic and membrane-bound ... Intracellular calcium levels, monitored by the ratiometric fluorescent calcium indicator fura-2, increased within seconds of ...

Furthermore, plasma membrane proteins and intracellular proteins can be released into the extracellular space by regulated or ... We find that almost half of the human single-span membrane proteins contain a transmembrane helix that .... ,READ MORE, ... The transmembrane domains of membrane fusogenic proteins are known to contribute to lipid bilayer mixing as indicated by ... The binding of ligands to iGluR membrane receptors, small-molecule inhibitorss to .... ,READ MORE, ...

intracellular membranes. en. dc.subject. DNA primers. en. dc.subject. chlamydia trachomatis. en. ...

Boni, R., Gallo, A. & Cecchini, S. (2017). Kinetic activity, membrane mitochondrial potential, lipid peroxidation, ... intracellular pH and calcium of frozen/thawed bovine spermatozoa treated with metabolic enhancers. Andrology, 5(1), pp.133-145. ...

Intact intracellular virions were found within ciliated cells in compartments with a single membrane bearing S glycoprotein. ... extracellular virions were seen associated with microvilli and the apical plasma membrane but rarely with ciliary membranes. ... Key SARS-CoV-2 entry molecules, ACE2 and TMPRSS2, were found to be localised to the plasma membrane including microvilli but ... Profiles indicative of viral fusion where tomography showed that the viral membrane was continuous with the apical plasma ...

Isolated cells were further fractionated into nuclear, cytosolic and membrane constituents to assess the intracellular ... After a 10-mg/kg i.v. bolus, intracellular drug levels where maximal after 8 hr and diminished significantly thereafter, ... Nonparenchymal cells contained abundant nuclear, cytosolic and membrane drug levels over a wide dose range. In contrast, at ... For this reason, we examined the intracellular fate of ISIS 1082, a 21-base heterosequence phosphorothioate ...

... interaction with intracellular molecules, changes in membrane functioning, and stopping cell cycle[18]. Schiff bases are often ... Changes in the cell membrane, especially blebbing and blistering which is occurring in the cell membrane, were observed in some ... Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. In the ... MDR-associated protein 1 is a protein that has a big impact on the intracellular availability of MDR-prone medicines[6,7]. MDR ...

Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining ... Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. ...

Many intracellular pathogens rely on host cell membrane compartments for their survival. The strategies they have developed to ... The obligate intracellular bacteria Chlamydia replicate within an intracellular vacuole, termed an inclusion. A large family of ... subvert intracellular trafficking are often unknown, and SNARE proteins, which are essential for membrane fusion, are possible ... A subset of SNAREs is specifically recruited to the immediate vicinity of the inclusion membrane, and their accumulation is ...

Intracellular Fluid [A11.284.835.450] * Intracellular Membranes [A11.284.835.514] * Mitochondrial Membranes [A11.284.835.514. ... Erythrocyte Membrane [A11.284.149.356] * Intracellular Membranes [A11.284.149.450] * Mitochondrial Membranes [A11.284.149.450. ... Membranes, Intracellular NLM Classification #. QU 350. Previous Indexing. Membranes (1966-1978). See Also. Vesicular Transport ... Intracellular Membranes Preferred Term Term UI T022294. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1979). ...

Organization of scramblases in intracellular membranes. ( a ) Scramblase m... in The role of lipid scramblases in regulating ... Scramblases are not only found in the PM, but also in intracellular membranes. Activity of scramblases are under strict ... b ) Cooperation between scramblases a... More about this image found in Organization of scramblases in intracellular membranes ... Figure 4. Organization of scramblases in intracellular membranes. ( a ) Scramblase may interact with components of the (glyco) ...

Caffeine acts through multiple mechanisms involving both action on receptors and channels on the cell membrane, as well as ... Caffeine (and theophylline) can freely diffuse into cells and causes intracellular calcium release (independent of ... as well as the intracellular Ryanodine receptor (RyR) which is the physiological target of cADPR (cyclic ADP-ribose), and cAMP- ... intracellular action on calcium and cAMP pathways. By virtue of its purine structure it can act on some of the same targets as ...

... which results in GLUT4 translocation from its intracellular pool to the plasma membrane and glucose transport into the cell (12 ... It is possible that additional factors also contribute to hepatocyte inflammatory pathway activation, such as intracellular ... the intracellular hepatocyte TG pool and the VLDL TG pool, which is subsequently secreted into the systemic circulation (51). ... which work in a paracrine manner to activate the intracellular proinflammatory pathways (e.g., JNK and IKK) in neighboring ...

Intracellular membrane structures are rare in bacteria, thus making them ideal chasses for cell-based vesicle production. The ... The density of the P9-specific membrane fraction was lower (approximately 1.13 g/cm3 in sucrose) than the densities of the ... Co-expression of P9 and P12 in a Pseudomonas host results in the formation of intracellular vesicles that are potential ... Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. ...

Cell Membrane & Intracellular Hydration. Measures the energetic effects of UMH treated water on the human body after ... Cell Membrane & Intracellular Hydration. Measures the energetic effects of UMH treated water on the human body after ...

LC3-dependent intracellular membrane tubules induced by γ-protocadherins A3 and B2: A role for intraluminal interactions. ... Basement membrane alterations in psoriasis are accompanied by epidermal overexpression of MMP-2 and its inhibitor TIMP-2. ...

Intracellular Membranes 25% * Circadian Clocks 24% * Long-Term Potentiation 22% * Plastics 19% ...

The authors suggest sanguinarine and NSC 631570 can alter intracellular membrane transport (231).. The publications mentioned ... It is well known all living cells have a membrane potential of about -60 to -100mV. The negative sign of the membrane potential ... More detailed investigations showed that it caused the depolarisation of mitochondrial membranes and as a result the activation ... surface of the cell membrane is relatively more negative than the than the immediate exterior surface of the cell membrane.. As ...

Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage ... Osmotic efflux of water leads to the increased concentration of intracellular solutes, causing potential cell damage due to ...

Lysosomes are membrane bound acidic intracellular organelles filled with hydrolytic enzymes that normally function as recycling ... Membrane topology of the Drosophila vesicular glutamate transporter JOURNAL OF NEUROCHEMISTRY Fei, H., Karnezis, T., Reimer, R ... Here we use a combination of molecular manipulations to increase expression of the NTT4/XT1 protein at the plasma membrane and ... Our data show that DVGLUT contains 10 hydrophobic regions that completely span the membrane (TMs 1-10) and that the amino and ...

Xiang Z, Kreisel F, Cain J, Colson A, Tomasson MH: Neoplasia driven by mutant c-KIT is mediated by intracellular, not plasma ... membrane, receptor signaling. Mol Cell Biol. 2007, 27: 267-282. 10.1128/MCB.01153-06. ... The major mutational hotspot in KIT is in exon 11, which encodes the juxtamembrane intracellular domain responsible for ...

... matrixand are formed by transmembrane adhesion proteins of the integrin family.On the intracellular side of the membrane, ... while their intracellular domains bindindirectly to bundles of actin filaments via the intracellular anchor proteinstalin, a- ... They interact through their extracellular domains to hold theadjacent plasma membranes together.The importance of desmosome ... and desmin filaments in heart muscle cells.The junction hasa dense cytoplasmic plaque composed of a complex of intracellular ...

... intracellular membrane-bounded organelle),GO:0043484(regulation of RNA splicing),GO:0045662(negative regulation of myoblast ... membrane),GO:0016021(integral to membrane),GO:0016324(apical plasma membrane). ... membrane),GO:0016021(integral to membrane),GO:0030593(neutrophil chemotaxis),GO:0032940(secretion by cell),GO:0048246( ... integral to membrane),GO:0016740(transferase activity),GO:0019370(leukotriene biosynthetic process),GO:0031965(nuclear membrane ...

... which are considered important for the cellular localization and intracellular trafficking of several membrane-bound proteins.5 ...

Baricitinib inhibits host intracellular membrane proteins AP2-associated protein kinase 1 (AAK1) and also binds cyclin G- ...