Cytoskeleton-associated plectin: in situ localization, in vitro reconstitution, and binding to immobilized intermediate...
The association and interaction of plectin (Mr 300,000) with intermediate filaments and filament subunit proteins were studied ... and binding to immobilized intermediate filament proteins.. R Foisner, F E Leichtfried, H Herrmann, J V Small, D Lawson, G ... These results suggest that plectin is a cross-linker of vimentin filaments and possibly also of other intermediate filament ... and binding to immobilized intermediate filament proteins.. R Foisner, F E Leichtfried, H Herrmann, J V Small, D Lawson, G ...http://jcb.rupress.org/content/106/3/723
New insights into peripherin expression in cochlear neurons - Lallemend François
en] Peripherin is an intermediate filament protein that is expressed in peripheral and enteric neurons. In the cochlear nervous ... The staining for both proteins was seen before birth within all cochlear neurons. By birth, and for 2 or 3 days, peripherin ... Both proteins were distributed along the entire neuronal length but exhibited complementary distribution, especially within the ...http://orbi.ulg.ac.be/handle/2268/707
anti-Vimentin antibody [VI-RE/1] (PE) | GeneTex
... and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is ... The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of ... Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell ... primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins ...http://www.genetex.com/Vimentin-antibody-VI-RE1-PE-GTX79851.html
Mitochondrial Dysfunction and Apoptosis Underlie the Pathogenic Process in α-B-Crystallin Desmin-Related Cardiomyopathy |...
3 Although the initial focus centered on mutations in the intermediate filament protein desmin, it has became apparent that ... and other proteins that interact with the intermediate filaments. The pathology is characterized by myofibril disruption that ... Intermediate filaments associate with mitochondria in many cell types, including skeletal and cardiac myocytes. In the last ... CryAB is a member of the small heat shock protein family, and, although it was originally classified as a lens protein, CryAB ...http://circ.ahajournals.org/content/112/22/3451.long
Relationship of Anti-vimentin antibodies to anti-endothelial antibodies - Open Medicine - Volume 1, Issue 3 (2006) - PSJD -...
The intermediate filament protein vimentin is a potential target antigen for autoantibodies in some infectious and autoimmune ... 8] M.W. Bohme, D.A. Evans and M.A. Miles: "Occurrence of autoantibodies to intermediate filament proteins in human visceral ... 9] A.M. Gown and A.M. Vogel: "Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors", Am. J. ... 18] J.L. Senecal, N.F. Rothfield and J.M. Oliver: "Immunoglobulin M autoantibody to vimentin intermediate filaments", J. Clin. ...http://psjd.icm.edu.pl/psjd/element/bwmeta1.element.-psjd-doi-10_2478_s11536-006-0028-5
... the gene encoding glial fibrillary acidic protein, the major intermediate filament protein of astrocytes in the central nervous ... Significance Statement: GFAP is an intermediate filament protein that is predominantly expressed in astrocytes of the central ... A key step in pathogenesis appears to be the accumulation of GFAP protein within astrocytes to excessive levels. Studies using ...http://www.eneuro.org/content/early/2015/09/15/ENEURO.0080-15.2015.1
Hitchhiker's Guide to the Galaxy on Keratin
Keratin filaments are intermediate filaments. Like all intermediate filaments, keratin proteins form filamentous polymers in a ... In many other cell types, such as cells of the dermis, keratin filaments and other intermediate filaments function as part of ... divergence of sequence but conservation of structure among intermediate filament proteins". Cell. 31 (1): 243-52. doi:10.1016/ ... By analysis of the primary structures of these keratins and other intermediate filament proteins, Hanukoglu and Fuchs suggested ...http://hitchhikersgui.de/Keratin
Desmin Antibody Review
the main intermediate filament (IF) protein of muscle cells. In skeletal muscle, desmin IFs form a scaffold that interconnects ... the intermediate filament (IF) protein occurring exclusively in muscle and endothelial cells. Desmin IFs are present throughout ... muscle-specific intermediate filament protein desmin is expressed in mononucleated myoblasts and in differentiated myotubes, ... distribution of the intermediate filament (IF) proteins desmin, keratin, and vimentin was studied immunohistochemically in ...http://antibodybeyond.com/reviews/antigen-d/desmin.htm
"Perinuclear localization and insulin responsiveness of GLUT4 requires " by Adilson L. Guilherme, Masahiro Emoto et al.
Disruption of intermediate filaments and microtubules in 3T3-L1 adipocytes by microinjection of a vimentin-derived peptide of ... Immunoelectron microscopy of the GLUT4-containing membranes also revealed their association with these cytoskeletal proteins. ... Using a novel method to purify intracellular GLUT4-enriched membranes, we identified by mass spectrometry the intermediate ... filament protein vimentin and the microtubule protein alpha-tubulin as components of these membranes. ...https://escholarship.umassmed.edu/oapubs/750/
Vimentin Levels and Serine 71 Phosphorylation in the Control of Cell-Matrix Adhesions, Migration Speed, and Shape of...
Metastasizing tumor cells show increased expression of the intermediate filament (IF) protein vimentin, which has been used to ... Long vimentin filaments were found in the proximity of large FAs. Vimentin expression in these cells caused a reduction in FAs ... Short fragments were mostly the size of a unit-length filament and were mainly localized close to small cell-matrix adhesions. ...http://kth.diva-portal.org/smash/record.jsf?pid=diva2:1173312
Dual Color Photoactivation Localization Microscopy of Cardiomyopathy-associated Desmin Mutants
Mutations in the DES gene coding for the intermediate filament protein desmin may cause skeletal and cardiac myopathies, which ... Morphological analysis of glutaraldehyde-fixed vimentin intermediate filaments and assembly-intermediates by atomic force ... Absence of intermediate filaments in a human adrenal cortex carcinoma-derived cell line.. Hedberg KK, Chen LB., Exp. Cell Res. ... The dynamic and motile properties of intermediate filaments.. Helfand BT, Chang L, Goldman RD., Annu. Rev. Cell Dev. Biol. 19 ...https://pub.uni-bielefeld.de/publication/2500819
Anti-Bovine GFAP Antibody | Bovine GFAP Antibody | GFAP Antibody
Glial fibrillary acidic protein (GFAP), a 50 kDa protein, is a major intermediate filament protein in mature astrocytes. It is ... RNA Extraction and Protein Analysis for Real-Time qPCR * Direct cDNA Synthesis from ... This product is an affinity-purified IgG antibody that recognizes GFAP protein. The antibody was raised in mouse using bovine ... GFAP, and can be used for immunohistochemical (IHC) detection of GFAP protein. ...http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Brain_and_CNS/GFAP?sitex=10020:22372:US&PEBCL1=2myUgR8sVr30sybI4SCtRDV5ck&PEBCL1_pses=ZG0ED6ED8A87D341DEF4AFB9A2C6AE35D119833A072EEEE0A4C71B7D4A288EA2C5B3FC9B13CDE4C10CD3B4489F6825B47A159032D424A9391B
Vimentin - Mashpedia Free Video Encyclopedia
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all ... intermediate filament. • extracellular matrix. Biological process. • intermediate filament organization. • movement of cell or ... SMAD protein signal transduction. • astrocyte development. • intermediate filament-based process. • positive regulation of gene ... Gene Music using Protein Sequence of VMAC "VIMENTIN-TYPE INTERMEDIATE FILAMENT ASSOCIATED COILED-CO" ...http://www.mashpedia.com/Vimentin
NEFM (Neurofilament Medium) Polypeptide, a Marker for Zona Glomerulosa Cells in Human Adrenal, Inhibits D1R (Dopamine D1...
... the type IV intermediate filament of mature neurons, one of the most abundant proteins in the nervous system. Neurofilament ... A 500 μg of whole-cell lysates protein with a final volume of 1 mL were pre-cleared by incubation with 10 μL of Protein A/G ... Interactions Between NEFM and D1R Proteins in H295R Cells. We found that endogenous D1R protein was localized in the cytoplasm ... Vector controls, pCMV6-AC-green fluorescent protein (Origene), and green fluorescent protein-tagged KCNJ5 wild type or mutants ...http://hyper.ahajournals.org/content/70/2/357
indirect fluorescent antibody technique
alpha-Internexin is the only neuronal intermediate filament expressed in developing cerebellar granule neurons. C L Chien. ... Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae. V C Cordes ... The heterotrimeric motor protein kinesin-II localizes to the midpiece and flagellum of sea urchin and sand dollar sperm. J H ... ACTIN-CAPPING PROTEIN INTERACTIONS IN MUSCLE. JAMES CASELLA; Fiscal Year: 1991. ..The long term goal of this proposal is to ...https://www.labome.org/topics/diagnostic/diagnosis/laboratory/immunologic/fluorescent/indirect-fluorescent-antibody-technique-16534.html
Intermediate Filament Proteins (ebook) by M Bishr Omary | 9780128034910
Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in ... download and read Intermediate Filament Proteins ebook online in EPUB or PDF format for iPhone, iPad, Android, Computer and ... Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in ... Intermediate Filament Proteins. by M Bishr Omary(ed.) ; Ronald K Liem(ed.) ...https://www.ebooks.com/2510239/intermediate-filament-proteins/omary-m-bishr-liem-ronald-k/
WikiGenes - SYNM - synemin, intermediate filament protein
Intermediate filament protein synemin is present in human reactive and malignant astrocytes and associates with ruffled ... Expression of the intermediate filament protein synemin in myofibrillar myopathies and other muscle diseases. Olivé, M., ... Desmuslin, an intermediate filament protein that interacts with alpha -dystrobrevin and desmin. Mizuno, Y., Thompson, T.G., ... Interactions of intermediate filament protein synemin with dystrophin and utrophin. Bhosle, R.C., Michele, D.E., Campbell, K.P ...http://www.wikigenes.org/e/gene/e/23336.html
JCI - Giant axonal neuropathy-associated gigaxonin mutations impair intermediate filament protein degradation
Intermediate filament proteins and their associated diseases. N Engl J Med. 2004;351(20):2087-2100.. View this article via: ... A 300,000-mol-wt intermediate filament-associated protein in baby hamster kidney (BHK-21) cells. J Cell Biol. 1985;100(2):620- ... Structure and assembly properties of the intermediate filament protein vimentin: the role of its head, rod and tail domains. J ... Giant axonal neuropathy-associated gigaxonin mutations impair intermediate filament protein degradation. Saleemulla Mahammad,1 ...https://www.jci.org/articles/view/66387
Neuroblastoma intermediate filament protein, LM Fluorescence - Stock Image - C032/0012 - Science Photo Library
... intermediate filament protein) fluorescently stained using a vimentin antibody. Note the nucleus in the centre of the ... Fluorescence light micrograph of a neuroblastoma cell (brain cancer cell) showing neurofilaments (intermediate filament protein ... Note the nucleus in the centre of the surrounded by thin intermediate filaments. Magnification: x64 when shortest axis printed ...https://www.sciencephoto.com/media/796938/view/neuroblastoma-intermediate-filament-protein-lm-fluorescence
Intermediate Filament-Associated Proteins | SpringerLink
10-nm-diameter cytoskeletal filaments that are distributed throughout the cytoplasm from the level of the nuclear surface to ... Intermediate Filament High Molecular Weight Protein Baby Hamster Kidney Intermediate Filament Vimentin Filament These keywords ... A novel intermediate filament-associated protein, NAPA-73, that binds to different filament types at different stages of ... Intermediate Filament-Associated Proteins. In: Goldman R.D., Steinert P.M. (eds) Cellular and Molecular Biology of Intermediate ...https://link.springer.com/chapter/10.1007/978-1-4757-9604-9_15
Genes coding for intermediate filament proteins: common features and unexpected differences in the genomes of humans and the...
Genes coding for intermediate filament proteins: common features and unexpected differences in the genomes of humans and the ... Genes coding for intermediate filament proteins: common features and unexpected differences in the genomes of humans and the ... Genes coding for intermediate filament proteins: common features and unexpected differences in the genomes of humans and the ... Genes coding for intermediate filament proteins: common features and unexpected differences in the genomes of humans and the ...http://jcs.biologists.org/content/early/2003/04/15/jcs.00444
The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin. | JCB
The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin.. A ... The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin. ... In previous studies we have characterized a lens-specific intermediate filament (IF) protein, termed filensin. Filensin does ... However, mixing of phakinin and filensin at an optimal ratio of 3:1 yields stable 10-nm filaments which have a smooth surface ...http://jcb.rupress.org/content/123/6/1507
The epidermal intermediate filament proteins of tunicates are distant keratins; a polymerisation-competent hetero coiled coil...
The coexpression of C and D in all epidermal cells and the obligatory heteropolymeric IF assembly of the recombinant proteins ... We discuss that type I to III IF proteins are expressed along the chordate branch of metazoa. ... Mutant proteins of Styela D and keratin 8 with a single cysteine in equivalent positions show that these chimeric IF are, like ... proteins (C and D) from the tunicate (urochordate) Styela are characterised as putative keratin orthologs. ...https://www.semanticscholar.org/paper/The-epidermal-intermediate-filament-proteins-of-are-Wang-Karabinos/65c887ea391fb54f81c08f14571f1fd48e9ceeab
Two Drosophila melanogaster proteins related to intermediate filament proteins of vertebrate cells - Semantic Scholar
... cells that form intermediate sized filaments in vertebrate cells. In indirect immunofluorescence, the group of cross reacting ... These antibodies reacted with the 46,000 and a 40,000 mol wt protein from Kc cells. Some antibodies showed cross-reaction with ... These cytoskeletal structures are heat-labile; the proteins in Kc or BHK cells rearrange after a brief heat shock, forming ... Monoclonal antibodies were prepared against a 46,000 mol wt major cytoplasmic protein from Drosophila melanogaster Kc cells. ...https://www.semanticscholar.org/paper/Two-Drosophila-melanogaster-proteins-related-to-in-Falkner-Saumweber/18f6d076c877d98f766c4356e407bf18eb0f19b0
Syncoilin is an intermediate filament protein in activated hepatic stellate cells. - Nuffield Department of Clinical...
This shift in phenotype is accompanied by a change in expression of intermediate filament (IF) proteins. HSCs express a broad, ... In muscle, syncoilin was identified as an alpha-dystrobrevin binding protein with sequence homology to IF proteins. We ... Syncoilin protein was strongly upregulated during in vitro activation of HSCs and undetectable in hepatocytes and liver ... syncoilin is able to form filamentous structures or at least to closely interact with existing cellular filaments. ...https://www.ndcn.ox.ac.uk/publications/429504