E-P) Confocal sections of in situ hybridisation labelling against olfactory marker protein (omp) in wild-type (E,I,M), neurod4 ... BrdU incorporation was detected by immunohistochemistry using an anti-BrdU antibody (G3G4, 1/500, DSHB). Confocal acquisitions ... E-P) Confocal sections of in situ hybridisation labelling against olfactory marker protein (omp) in wild-type (E,I,M), neurod4 ... In situ hybridisation and immunostaining. In situ hybridisation was performed as previously described (Oxtoby and Jowett, 1993 ...

Nucleic Acids Symp. Ser. No. 50. Pages: 69-70 *. Description. 「研究成果報告書概要(和文)」より ... Mutagenic Incorporation of Nucleoside Analogs by PolymerasesPrincipal Investigator. *. Principal Investigator. HAYATSU Hikoya ... MutS protein / Retrovirus / Replicational errors / Mismatch repair / 校正作用 / 転写エラー / RNAポリメラーゼ / error catastophe / mut:L / mutl ... Microtiter plate-hybridization / 水流強度 / 多環芳香族 / モニタリング / Cell Death /

Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells. Nucleic Acids Res. 2002;30(4):1009-15 ... Parra I, Windle B. High resolution visual mapping of stretched DNA by fluorescent hybridization. Nat Genet. 1993;5(1):17-21. ... mis-incorporation of nucleotides, and limited resources, to name a few [6]. With many potential causes of replication stress, ... Protein concentrations were determined via DC protein assay (Bio-Rad), and 100 μg of protein was added to IgG control beads ( ...

A heat shock protein (HSP20) may take part in the physiological response to BPH infestation, making it a potential target for ... Over 4900 proteins were identified from these three rice cultivars using iTRAQ proteomics study. A total of 414, 425 and 470 ... In the case of the resistant rice cultivar (PR), 6 DEPs, i.e. two lipoxygenases (LOXs), a lipase, two dirigent proteins (DIRs) ... A two-component response regulator protein (ORR22) may participate in the early signal transduction after BPH infestation. ...

Frey, T. 1994: Detection of bromodeoxyuridine incorporation by alteration of the fluorescence from nucleic acid binding dyes ... nucleic acid hybridization and polymerase chain reaction compared. Clinical Chemistry 39(9): 1980-1982 ... Aoki, T.; Satoh, M.; Watabe, H. 1998: Detection of blotted antigen using a fusion protein between protein A and pepsinogen C. ... Sobol, R.E.; Green, M.R.; Kawasaki, E.S. 1996: Detection of carcinoma metastases by nucleic acid amplification. Official ...

MS analysis of single-nucleotide differences in circulating nucleic acids: application to noninvasive prenatal diagnosis. ... Harun, Sulaiman Wadi and Ahmad, Harith (2004) Gain clamped double-pass L-band EDFA with incorporation of FBG at the input end ... Chee, H.Y. and AbuBakar, Sazaly (2004) Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that ... a study using a fluorescent in situ hybridisation technique. Virchows Archiv, 445 (5). pp. 506-514. ISSN 0945-6317, DOI https ...

Furthermore, Smad proteins, transcriptional effectors of TGF-β signaling, are co-expressed and physically interact in the basal ... We also show that Dlx and Smad proteins localize to promoters/enhancers of a number of common telencephalic genes in vivo and ... that Smad proteins co-activate transcription with Dlx family members, except with certain mutated human DLX proteins identified ... Here we show that components of both bone morphogenetic protein/growth differentiation factor and TGF-β/activin/Nodal branches ...

Integration occurs not only in somatic cells, but also in germ cells (see Unregulated Hazards: Naked and Free Nucleic Acids ... Current computation cannot handle the dynamics of one single protein folding, even given all the information on the amino-acid ... Analysis of these short transcripts by Northern hybridization, RT-PCR (reverse transcription followed by polymerase chain ... Somaclonal variation (SCV) in transgenic plants may slow incorporation of introduced genes into commercially competitive ...

... and homoduplex hybridization and nuclease sensitivity. Nucleic Acids Res. 1995, 23: 2019-2024. 10.1093/nar/23.11.2019. ... Sossey-Alaoui K, Downs-Kelly E, Das M, Izem L, Tubbs R, Plow EF: WAVE3, an actin remodeling protein, is regulated by the ... The inherent instability of single-stranded oligonucleotides has been circumvented by the incorporation of chemical ... Locked nucleic acid (LNA) constructs. Locked nucleic acid (LNA) constructs represent a novel class of nucleic acid analogs ...

Valrubicin is a semisynthetic analog of doxorubicin that inhibits incorporation of nucleosides into nucleic acids. It is ... FGFR3 protein expression and its relationship to mutation status and prognostic variables in bladder cancer. J Pathol. 2007 Sep ... Use of fluorescence in situ hybridization to predict response to bacillus Calmette-Guérin therapy for bladder cancer: results ... Folic acid and vitamin B12 are typically given prior to initiation of treatment. Dexamethasone is also given with pemetrexed, ...

It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations ... a web resource for human protein-protein interactions. ... Negative Regulation Of Nucleic Acid-templated Transcription. * ... arranged blue cauda cd99 cords equina focal fusion glomus hybridization immunophenotypic indolent infrequent involving ki ... Molybdenum Incorporation Into Molybdenum-molybdopterin Complex. *Developmental Pigmentation. *Positive Regulation Of Cellular ...

Nucleic acids research 2013;41:8853-71.. 3.Parkin DM, Stiller CA, Nectoux J. International variations in the incidence of ... Overexpression of p53 protein in primary Ewings sarcoma of bone: relationship to tumor stage, response and prognosis. Abudu, A ... Genetic imbalances revealed by comparative genomic hybridization in Ewing tumors. Ozaki, T, et al. 2001, Genes Chromosomes and ... rapid incorporation as autograft and easy accessibility without significant donor site morbidity. Structural change is also ...

... carbohydrates furnish the energy nec-essary for maintenance of the cell in replacement of the proteins and ribo-nucleic acids ... reaction producing oxaloacetate from pyruvate and CO2 serves to supply additional oxaloacetate for incorporation into the TCA ... biological significance because they enable a yeast species to exploit fully the evolutionary processes such as hybridization ... In synthetic media the inclusion of 9 vitamins-biotin, pantothenic acid, folic acid, niacin, para-aminobenzoic acid, inositol, ...

An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat ... Total translation (incorporation of OPP) can be measured from fluorescence levels by conjugating fluorophores using click ... such as in situ hybridization, and unbiased approaches based on RNA sequencing (RNAseq) (Goldman et al., 2017; Honkoop et al., ... A core group of amino acids shared over 500 million years of evolution forms an interface along the protein surface, suggesting ...

Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery (505 views). Bioconjug Chem ( ... Site-specific protein double labeling by expressed protein ligation: applications to repeat proteins (431 views). Org Biomol ... Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid (501 views). J ... Dabpna: Design, Synthesis And Nucleic Acids Binding Studies (437 views). Nucleosides Nucleotides Nucleic Acids (ISSN: 1525-7770 ...

Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within five days. DNA-stable isotope ... Additionally, higher transcription levels for chitin, nucleic acid, and peptidoglycan degrading enzymes were found in long-term ... NanoSIMS confirmed incorporation of 13C into individual bacterial cells of Fusibacteraceae sorted from microcosms. Genomes of ... in complex microbial communities when coupled with fluorescence in situ hybridization or downstream omics analyses. We have ...

As a result, the relative rates of amino acid replacement among sites within a protein can vary significantly. Previous large- ... to the overall efficiency of amino acid incorporation. The efficiencies of amber codon reassignments were quantified at eight ... disordered-structured sites are involved in nucleic acid binding. We also observe notable differences in the statistical trends ... The molecular approach involved bacterial artificial chromosome (BAC) library screening, fluorescent in situ hybridization, ...

Stoler, D.L.; Manly, K.F. 1989: Transformed cells overexpress a cytosolic nucleic acid binding protein. Journal of Cellular ... Fundora, T.; Garcia, Y.; Roque, M.C.; Vidal, H.; Ferrer, R.; Gonzalez, A.I. 1991: Transferrin incorporation into the erythron ... Transfer of resistance against Phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection ... Transforming growth factor-alpha and epidermal growth factor messenger ribonucleic acid and protein levels in human placentas ...

amino acid residues which serves to direct translocation. Of 26 excreted mammalian proteins cited in document (63), 25 are ... whereby said micro-organism is transformed by the incorporation of. (a) a ds-rDNA coding for preprochymosin, prochymosin, ... The successful screening of positive clones by differential colony hybridization to two mRNA populations respectively ... Nucleic acids and antibodies to fight coronavirus * Diagnostics and analytics * Go back ...

Enumeration of small ciliates in culture by flow cytometry and nucleic acid staining. Journal of Microbiological Methods. 49: ... Smith, R. W., Houlihan, D. F., Nilsson, G. E. and Brechin, J. G. (1996) Tissue specific changes in protein synthesis rates in ... Contrasting activity patterns determined by BrdU incorporation in bacterial ribotypes from the Arctic Ocean in winter. Front. ... Identification of microorganisms in hydrocarbon contaminated aquifer samples by fluorescence in-situ hybridization (CARD-FISH ...

This is the image of an interaction between water and large molecules, such as proteins and nucleic acids, with the system--the ... Surprisingly, hybridization to blots of brain RNA showed an approximately 25 fold lower level of transcripts from these Alu ... Butler was defending the idea of biological intelligence, the incorporation of experience into physiology and heredity. ... The center of gravity in the study of the nucleic acids has now shifted from heredity to development. Almost nothing remains of ...

... vivo Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids ... Enrichment of skeletal stem cells from human bone marrow using spherical nucleic acids Healthy aging and the blood brain ... chromatin-mediated transcriptional response Single-cell in situ RNA profiling by sequential hybridization Small-molecule-driven ... glutamic acid 195-mediated catch-slip bonds Cell-free protein synthesis and assembly on a biochip Forebrain engraftment by ...

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