Loading...
*  Peptide Nucleic Acids - CHEMICAL BIOLOGY
... - reflects the multidimensional character of chemical biology, focusing in particular ... Peptide nucleic acids (PNAs) containing thymine monomers derived from chiral amino acids: Hybridization and solubility ... in 1991 from the University of Copenhagen (1). The structure is reminiscent of both proteins and nucleic acids, hence the name ... On the other hand, incorporation of positively charged amino acid side chains, such as lysine or arginine, led to the ...
http://schoolbag.info/chemistry/chemical_biology/128.html
*  Patent US8497064 - Nucleic acid labelling compounds - Google Patents
The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a ... Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a ... nucleic acid labeling compounds of the invention with a nucleic acid and the hybridization products comprising the nucleic acid ... Such a compound should be suitable for enzymatic incorporation into a nucleic acid. Furthermore, the nucleic acid to which the ...
http://www.google.com.au/patents/US8497064
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... including sample nucleic acid extraction, PCR amplification using random primers, fluorescent dye incorporation, and microarray ... Protein, Protein Binding, Computational Biology, Drug Design, optimization (mathematics), Amino Acids, Peptides, and Proteins, ... hybridization, scanning, and analysis.. Immunology, Issue 50, virus, microarray, Virochip, viral detection, genomics, clinical ... The aim of de novo protein design is to find the amino acid sequences that will fold into a desired 3-dimensional structure ...
https://www.jove.com/visualize/abstract/23185364/ezrin-interacts-with-sars-coronavirus-spike-protein-restrains
*  Nano-sensor array - The Board of Trustees of the Leland Stanford Junior University
Edman, et al.,Electric field directed nucleic acid hybridization on microchips.,Nucleic Acids Res.,Dec. 15, 1997,25 (24),4907- ... USE OF ELECTRICAL FIELDS TO CONTROL INTERACTIONS BETWEEN PROTEINS AND NUCLEIC ACID CONSTRUCTIONS IMMOBILIZED ON SOLID SUPPORTS ... Efficient incorporation of positively charged 2′, 3′-dideoxynucleoside-5′-triphosphates by DNA polymerases and their ... NUCLEIC ACID AMPLIFICATION. 2013-10-24. Li et al.. 20130231254. METHOD OF NUCLEIC ACID AMPLIFICATION. 2013-09-05. Kawashima et ...
http://www.freepatentsonline.com/10059982.html
*  С ?? ҩ ??.??Ϣ?? .11369501??11370000
11369563:A novel LIM protein Cal promotes cardiac differentiation by associatio. *11369564:Easily denaturing nucleic acids ... 11369810:Topological constraints in nucleic acid hybridization kinetics. *11369811:Solving the riddle of codon usage ... 11369815:HYPROSP: a hybrid protein secondary structure prediction algorithm a k. *11369816:Incorporation of non-nucleoside ... 11369821:MAO: a Multiple Alignment Ontology for nucleic acid and protein sequen ...
http://www.100md.com/index/other/byinfono/022/740.htm
*  Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin:...
Digoxigenin is linked to dUTP via an 11-atom linear spacer (Dig-[11]-dUTP). Following hybridization of membrane-bound target- ... DNA is modified with the cardenolide-hapten digoxigenin by enzymatic incorporation of digoxigenin-labeled deoxyuridine- ... An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo.. *Bok Hee C Jenke, Christian P Fetzer, +5 ... Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: ...
https://www.semanticscholar.org/paper/Non-radioactive-labeling-and-detection-of-nucleic-A-Kessler-H%C3%B6ltke/922f23600982d8de8db02710bf006a436cd3f4e5
*  Professor Bill Keevil | Biological Sciences | University of Southampton
A fluorescence in situ hybridization method using a peptide nucleic acid probe for the identification of Salmonella spp. in a ... Giao, M. S., Azevedo, N. F., Wilks, S. A., Vieira, M. J., & Keevil, C. W. (2010). Effect of chlorine on incorporation of ... Secker, T. J., Hervé, R., & Keevil, C. W. (2011). Adsorption of prion and tissue proteins to surgical stainless steel surfaces ... Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH). ...
https://www.southampton.ac.uk/biosci/about/staff/cwk.page
*  Patent US7144700 - Photolithographic solid-phase polymer synthesis - Google Patents
The protein, labeled directly or indirectly, can be added to the chip during or after hybridization of target nucleic acid, and ... For instance, structural groups are optionally added to the ribose or base of a nucleoside for incorporation into an ... Polynucleotide or nucleic acid probes have long been used to detect complementary nucleic acid sequences in a nucleic acid of ... The probes in hybridization assays can be single stranded or double stranded nucleic acids, polynucleotides or polynucleotides ...
http://www.google.com/patents/US7144700?dq=%22robert+sheehan%22
*  Enzymatic Incorporation of an Antibody-Activated Blue Fluorophore into DNA.
These include the enzymatic incorporation of fluorophore-conjugated deoxynucleotide triphosphates into nucleic acids by such ... Aside from their benefit to studies of the dynamics of protein-ligand interactions, these antibodies can be envisioned in a ... Significant progress continues in the discovery of new hybridization assays and in the improvement of those already in use.[3] ... a) D. K. Braithwaite, J. Ito, Nucleic Acids Res. 1993, 21, 787 - 802; b) J. Filee, P. Forterre, T. Sen-Lin, J. Laurent, J. Mol ...
https://www.docme.ru/doc/1933511/enzymatic-incorporation-of-an-antibody-activated-blue-flu..
*  Kits for Labeling Proteins and Nucleic Acids-Section 1.2 | Thermo Fisher Scientific - CN
Our labeling kits provide a convenient way for labeling small amounts of purified proteins and antibodies with the highly ... Detailed protocols for preparing fluorescent DNA hybridization probes for chromosome in situ hybridization and dot-blot ... The labeling protocols provided generally result in incorporation of about one dye per 12-15 bases, which we have determined to ... Kits for Labeling Nucleic Acids with a Fluorescent Dye ULYSIS Nucleic Acid Labeling Kits. The ULYSIS Alexa Fluor Nucleic Acid ...
https://www.thermofisher.com/cn/zh/home/references/molecular-probes-the-handbook/fluorophores-and-their-amine-reactive-derivatives/kits-for-labeling-proteins-and-nucleic-acids.html
*  US6872527B2 - Nucleic acid archiving - Google Patents
... hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to ... These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration ... Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the ... and enable the interface of solid phase bound nucleic acid with enzyme, ...
https://patents.google.com/patent/US6872527B2/en
*  MHY1 Encodes a C2H2-Type Zinc Finger Protein That Promotes Dimorphic Transition in the Yeast Yarrowia lipolytica
Nucleic acid manipulation. Genomic DNA, plasmid DNA, and total RNA were prepared from Y. lipolytica, as described previously (1 ... Synthesis in vitro of Mhy1p was assessed by incorporation of L-[35S]methionine, sodium dodecyl sulfate-polyacrylamide gel ... Hybridization, stringency of washes, and signal generation and detection were performed as recommended by the manufacturer. ... contained 855 bp coding for a 285-amino-acid protein, Mhy1p, with a predicted molecular mass of 32,636 Da (Fig. ​(Fig.2A).2A). ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC93759/?lang=en-ca
*  Neutrophil inhibitors - Patent # 5708141 - PatentGenius
A) Cloning by Nucleic Acid Hybridization. Total RNA is prepared from hookworms by guanidinium thiocyanate extraction (McDonald ... hookworm nucleic acid.. In addition, the above-described isolated pure protein can be used to form antibodies by standard ... to oxidize carbohydrates to aldehydes which were thenreacted with biotin-hydrazide leading to incorporation of biotin into any ... The nucleic acid molecule is expressed in a cultured host cell transformedwith a vector containing the nucleic acid molecule ...
http://www.patentgenius.com/patent/5708141.html
*  Patent US7977108 - Method for detecting a mutation in a repetitive nucleic acid sequence - Google Patents
The present invention provides methods and probe nucleic acids for detecting mutant forms of target nucleic acids that comprise ... Polymorphism at Codon 129 of the Human Prion Protein my Melting Point Analysis of Fluorescently labeled Hybridization Probes. ... A "nucleotide incorporating biocatalyst" catalyzes the incorporation of nucleotides into a nucleic acid. Nucleotide ... The probe nucleic acids, target nucleic acids, and/or amplicons of the target nucleic acids (e.g., via primer nucleic acids ...
http://www.google.com/patents/US7977108?dq=645576
*  MCAT - Biology Flashcards by Caitlin Rodeghier | Brainscape
Consists of a capsid protein coat that contains the nucleic acid (either DNA or RNA, never both). Most animal viruses surround ... of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.. vs. ... for incorporation into a protein. ... Then the nucleic acid of the virus penetrates into the cell. ... Important proteins (mostly formed in liver, act as source of amino acids for tissue protein replacement):. 1. "albumin": ...
https://www.brainscape.com/flashcards/mcat-biology-422004/packs/486133
*  Nucleoside and oligonucleotide analogues - Patent # 7314923 - PatentGenius
Wengel, "LNA (Locked Nucleic Acids) : Synthesis and High-Affinity Nucleic Acid Recognition--Stop the Twisting!", National ... Acid Res., 1990, vol. 18, No. 13, pp. 3777-3783. cited by other.. Oberhauser et al, "Effective incorporation of 2'O-methyl- ... Acid Res., 1995, vol. 23, No. 15, pp. 3050-3055. cited by other.. Shea et al, "Synthesis, Hybridization properties and ... Gum et al, "Stimulation of 92-kDa Gelatinase B Promoter Activity by ras Is Mitrogen-activated Protein Kinase . . . ", J. Biol. ...
http://www.patentgenius.com/patent/7314923.html
*  Patente US6025194 - Nucleic acid sequence of senescence asssociated gene - Google Patentes
Isolated, purified, and recombinant nucleic acids and proteins corresponding to the human GC6 gene and its mRNA and protein ... products, as well as peptides and antibodies corresponding to the GC6 protein can be used to identify senescent cells, ... in Nucleic Acid Hybridization (1985).. "Encoding" refers to an inherent property of a sequence of nucleotides in a nucleic acid ... Incorporation of a label can be achieved by a variety of means, ie., by use of radiolabeled or biotinylated nucleotides in ...
http://www.google.es/patents/US6025194
*  Tim Stearns | Stanford Medicine Profiles
We present an alternative approach in which identification of nucleic acids is accomplished by direct hybridization to high- ... We report that Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein ... Third, we found that the amino and carboxyl termini of alpha-tubulin were necessary for incorporation of the alpha-beta-tubulin ... The centriolar satellite proteins Cep72 and Cep290 interact and are required for recruitment of BBS proteins to the cilium ...
https://med.stanford.edu/profiles/tim-stearns
*  Patente US6001610 - Method for the particularly sensitive detection of nucleic acids - Google Patentes
... nucleic acid A with a probe nucleic acid B which contains a sequence B1 which base pairs with a part of the target nucleic acid ... with a template nucleic acid C containing a sequence C2 which base pairs with a part of the cleavage product B' and a sequence ... cleaving the hybridized probe nucleic acid B to produce a cleavage product B' containing the sequence B2, hybridizing the ... C1 which does not hybridize with the sequence B1 of the probe nucleic acid B, extending the hybridized cleavage product B' with ...
http://www.google.es/patents/US6001610?hl=es&dq=flatulence
*  Colorimetric Sandwich Assays for Nucleic Acid Detection | Springer for Research & Development
Colorimetric sandwich assay has attracted wide interests in detection of nucleic acid due to the advantages of convenience and ... implications for nucleic acid analysis by hybridisation. Nucleic Acids Res 28:3904-3909CrossRefGoogle Scholar ... Xie XJ, Xu W, Liu XG (2012) Improving colorimetric assays through protein enzyme-assisted gold nanoparticle amplification. Acc ... Nguyen HK, Southern EM (2000) Minimising the secondary structure of DNA targets by incorporation of a modified deoxynucleoside ...
https://rd.springer.com/chapter/10.1007/978-981-10-7835-4_6
*  Группа биоконъюгации - ИБХ РАН
Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3- ... pyrene-functionalized locked nucleic acid (LNA) monomers M(1)-M(6) and their incorporation into DNA oligomers is described. ... Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we ... Highly fluorescent conjugated pyrenes in nucleic acid probes: (phenylethynyl)pyrenecarbonyl-functionalized locked nucleic acids ...
http://www.ibch.ru/ru/structure/groups/conjugation
*  Assistant Engineering resume in Houston, TX - October 2018
... protein, cancer, molecular, phd, biology, scientist, biomedical, microbiology, anderson ... Self-Renewal assays, Brdu Incorporation/Proliferation Assays, Luciferase Assays.. Molecular Biology: Nucleic acid isolation ... Northern hybridization with radioactively labeled P32, Southern Blotting, microRNA microarray and mRNA microarray analysis. ... Regulation of c-Myc protein stability by Rak tyrosine kinase, Cancer Biology Program Retreat 2011 (Poster presentation) ...
https://www.postjobfree.com/resume/ac7d5t/et-protein-cancer-molecular-houston-tx
*  LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PRIMER-LINKED LINEAR PROBES - Patent application
... target proteins present in non-amplified sample instead of nucleic acid probes designed to hybridize to target nucleic acid ... 0308] Incorporation of a photodiode 184 directly beneath each hybridization chamber 180 allows the volume of probe-target ... nucleic acid extraction 290, nucleic acid incubation 291, nucleic acid amplification 292 and detection and analysis 294. [0262] ... to specific nucleic acid sequences for use in nucleic acid tests. If hybridization occurs, then the complementary sequence is ...
http://www.patentsencyclopedia.com/app/20110312541
*  Patent US5639853 - Respiratory syncytial virus vaccines - Google Patents
... a neutralizing and fusion epitope of the Respiratory Syncytial Virus fusion protein or a neutralizing epitope of the G protein. ... protein related proteins polypeptides and peptides of the present invention may be used as probes in nucleic acid hybridization ... is mixed with baculovirus helper DNA and used to transfect insect cells in culture to effect recombination and incorporation of ... Finally, the fusion protein and G protein related polypeptides or proteins of the invention, like the bonafide viral proteins, ...
http://www.google.co.uk/patents/US5639853
*  Patent US5856174 - Integrated nucleic acid diagnostic device - Google Patents
The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo ... The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention ... the nucleic acid sample is incubated with the oligonucleotide array in the hybridization chamber. Hybridization between the ... Liberation of nucleic acids from the sample cells or viruses, and denaturation of DNA binding proteins may generally be ...
http://www.google.com.au/patents/US5856174