SYP-3 Restricts Synaptonemal Complex Assembly to Bridge Paired Chromosome Axes During Meiosis in Caenorhabditis elegans |...
Fluorescence in situ hybridization and time-course analysis of chromosome pairing:. 5S rDNA probe, X left end probe derived ... Homologous pairing was monitored quantitatively as in MacQueen and Villeneuve (2001), with fluorescence in situ hybridization ( ... SYP-3 is not detected on chromosomes in situ on syp-3(ok758) mutants when using either the α-SYP-3 C-terminal or α-SYP-3 N- ...
Dynamic Changes in the Distribution of a Satellite Homologous to Intergenic 26-18S rDNA Spacer in the Evolution of Nicotiana |...
Fluorescence in situ hybridization: Fluorescence in situ hybridization (FISH) was carried out as described in Lim et al. (2000b ... Fluorescence in situ hybridization (FISH) and Southern blot analysis revealed its occurrence as an independent satellite ( ... For FISH we conducted simultaneous hybridization of the IGS probe (for A1/A2 units, green fluorescence) and pTa71 (for rDNA ... green fluorescence) IGS probe against the A1/A2 repeats and biotin-labeled (Cy3 detected, red fluorescence) pTa71 for 35S rDNA ...
Chromosome Rearrangements Induce Both Variegated and Reduced, Uniform Expression of Heterochromatic Genes in a Development...
... we performed in situ hybridization to lt mRNA in imaginal discs using fluorescence detection and confocal microscopy. We ... In situ hybridization to the polytene salivary glands revealed the nascent lt transcript in each nucleus of lt+/Df glands (A). ... In situ hybridization to lt mRNA of leg discs from larvae bearing a lt-variegating rearrangement showed a reduced, ... In situ hybridization to whole mount third instar larval tissues: For a typical experiment, at least 15 wandering third instar ...
Integrating Genetic Linkage Maps With Pachytene Chromosome Structure in Maize | Genetics
... and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152: ... and autoradiography yields results that are comparable to fluorescence in situ hybridization (FISH). In addition, the observed ... In situ hybridization: The bacterial artificial chromosome (BAC) 60.j16 (encompassing the marker crcbr58r with a genetic map ... The predicted locations of seven markers on chromosome 9 were tested using in situ hybridization (ISH) of single-copy DNA ...
Table of Contents | Genetics
... and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization ...
A High-Density Genetic Recombination Map of Sequence-Tagged Sites for Sorghum, as a Framework for Comparative Structural and...
... synaptonemal complex spreads with genetically mapped probes or their corresponding BACs by fluorescence in situ hybridization ( ... Co-hybridization of these probes with genetically mapped RFLPs has associated concentrations of centromeric repeats with marker ... Duplicate probes were removed from the map by inspection of genomic hybridization patterns for cosegregating loci and also by ... 1998 An anchored framework BAC map of mouse chromosome 11 assembled using multiplex oligonucleotide hybridization. Genomics 54 ...
Extensive Sex-Specific Nonadditivity of Gene Expression in Drosophila melanogaster | Genetics
Each of the 56 hybridization samples was subjected to linear normalization in PROC MIXED with the model where i (= 1-28) ... The expression platform that we present is a custom-designed long-oligonucleotide microarray printed in situ by Agilent ... loess-transformed log base 2 fluorescence intensity measurements. Two-step mixed model analysis of variance (Wolfinger et al. ... All hybridizations were performed at the Agilent facility in Palo Alto, California, according to standard protocols. ...
Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration Is Mediated by Signaling Through the Relaxin...
In situ expression analysis. PGs were dissected and fixed in 4% paraformaldehyde and then RNA in situ hybridization was ... DAF2-DA fluorescence was quantified in ImageJ (National Institutes of Health) by measuring the mean gray value of each lobe of ... we performed in situ hybridization using a probe that hybridizes to the Lgr3 transcript and were able to observe a specific ... Hybridization was performed under conditions to produce maximal signal specificity. PG tissues were stained for the same ...