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Circadian Clock NAD+ Cycle Drives Mitochondrial Oxidative Metabolism in Mice | Science
MCAD was eluted by boiling in 1× SDS-PAGE loading buffer followed by immunoblotting for MCAD (Santa Cruz) and acetylated lysine ... Proteins were separated by two-dimensional gel electrophoresis and subjected to immunoblotting using antibody to acetyl-lysine ... Protein Gel Electrophoresis and Immunoblotting. Protein from liver whole tissue, mitochondria, and nuclei was diluted in ... SDS-PAGE loading buffer followed by immunoblotting for OTC (Abcam) and acetylated lysine [generated in J. Denu laboratory (25 ...https://science.sciencemag.org/content/342/6158/1243417?ijkey=6c9f0e998a106b9e20046fb4e7190067aa89650a&keytype2=tf_ipsecsha
TGF-β1 induction of the adenine nucleotide translocator 1 in astrocytes occurs through Smads and Sp1 transcription factors |...
Immunoblotting. Total proteins were purified from nitrocellulose filter implants, uninjured cortex and primary astrocyte ...https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-5-1
Insight into the role of alternative splicing within the RBM10v1 exon 10 tandem donor site | BMC Research Notes | Full Text
Immunoblotting. Western blotting was carried out as previously described . RBM10 antibody was used at a dilution of 1:500. ...https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-0983-5
PINK1-dependent Parkin E3 activity assay | Open Biology
Immunoblotting. Samples were subjected to SDS/PAGE (8-14%) and transferred onto nitrocellulose membranes. Membranes were ... Miro1, ubiquitin and PINK1 were detected by immunoblotting using anti-SUMO, anti-FLAG and anti-MBP antibodies, respectively. ... Ubiquitylation reactions were subjected to immunoblotting with anti-FLAG antibody (Sigma, 1 : 7500), anti-Parkin, anti-SUMO1 or ... MBP-PINK1 undergoes ubiquitylation that can be observed in a bandshift detectable by anti-ubiquitin immunoblotting and that is ...http://rsob.royalsocietypublishing.org/content/4/3/130213
Combination of the ABL kinase inhibitor imatinib with the Janus kinase 2 inhibitor TG101348 for targeting residual BCR-ABL...
Immunoblotting. Immunoblotting was performed as described previously . After different treatments were performed as ... D) K562 cells were treated with TG101348 for 24 h, and total extracts were examined by immunoblotting using anti-phospho ABL, ... Cell lysates were analyzed by immunoblotting 72 h after transfection using Jak2 and β-actin Abs. Blots were scanned and optical ... Cell lysates were analyzed by immunoblotting 72 h after transfection using an anti-JAK2 Ab. We confirmed that Jak2 expression ...https://jhoonline.biomedcentral.com/articles/10.1186/1756-8722-7-37
Cyclin K and cyclin D1b are oncogenic in myeloma cells | Molecular Cancer | Full Text
Immunoblotting. Methods for protein extraction, SDS-PAGE and immunoblotting were described previously . ... This was verified by immunoblotting (Figure 4b). The STAT3 pathway is constitutively activated in LP-1 cells. In LP-1K cells, ...https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-9-103
Cell surface heparan sulfate proteoglycans contribute to intracellular lipid accumulation in adipocytes | Lipids in Health and...
Immunoblotting. Cell lysates were prepared with 20 mM Tris pH 7.4, 150 mM NaCl (TBS) containing 1% (v/v) Triton-X100. For ... is quantitatively consistent with that measured by immunoblotting. When 125I-RAP was incubated with cells at 37°C to evaluate ...https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-4-2
Identification and characterization of a novel gene, c1orf109, encoding a CK2 substrate that is involved in cancer cell...
Immunoblotting. Cells were lysed in RIPA lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 % NP-40, 0.1 % SDS, and 0.5 % ... the expression of c1orf109 in 11 breast cancer cell lines and a melanoma cell line were detected by immunoblotting. Meanwhile, ...https://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-19-49
Functional characterization of Trip10 in cancer cell growth and survival | Journal of Biomedical Science | Full Text
Immunoblotting. Cell lysates were collected, and protein concentration was determined with a protein assay kit (Bio-Rad) using ...https://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-18-12
CiteSeerX - Citation Query Cleavage of structural proteins during the assemble of the head of bacteriophage T4.
and quantitative immunoblotting. Magnetic beads with and without bound constituents were fixed and prepared for electron ...http://citeseer.ist.psu.edu/showciting?cid=52905
In Vitro Enrichment of Tumor‐Initiating Cells from Human Established Cell Lines - Current Protocols
Basic Protocol 3: Phenotypic Identification of TICs by Immunoblotting. Materials * TIC‐low and TIC‐high cultured cells ( ... Basic Protocol 3: Phenotypic Identification of TICs by Immunoblotting. *Basic Protocol 4: Functional Identification of TICs by ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-sc0307.html
Extraction, Identification, and Quantification of Histones from Small Quantities of Specific Brain Tissue - Current Protocols
For a more detailed description of the immunoblotting protocol see unit 5.19. (Gallagher et al., ). ... Immunoblotting and immunodetection. Curr. Protoc. Neurosci. 29:5.19.1‐5.19.24. doi: 10.1002/0471142301.ns0519s29. ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ns0438.html?quicktabs_cp=toc
Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia - Volume 20, Number 4-April 2014 - Emerging...
Western Immunoblotting. The ptx, prn, and fha proteins were detected by Western immunoblotting as described (10,11,14). ...https://wwwnc.cdc.gov/eid/article/20/4/13-1478_article
The Cellulosome System of Acetivibrio cellulolyticus Includes a Novel Type of Adaptor Protein and a Cell Surface Anchoring...
Immunoblotting.Proteins were subjected to SDS-10% PAGE, and the separated proteins were then transferred onto a nitrocellulose ...https://jb.asm.org/content/185/15/4548?ijkey=4cff835d98a2674ea9f1f1e0e5f6290363be3f7a&keytype2=tf_ipsecsha
Comparison of Eleven Commercial Tests for Chlamydia pneumoniae-Specific Immunoglobulin G in Asymptomatic Healthy Individuals |...
Immunoblotting.C. pneumoniae HK92, a local isolate from a patient with chronic pharyngitis, was used as the antigen for the ... These six samples where further investigated by immunoblotting, which showed the reaction of human serum antibodies to 11 C. ... Sensitivity and specificity.On the basis of our internal gold standard and the results of immunoblotting analysis, we ... Six samples that had false-positive results by some assays were further investigated by immunoblotting, which classified all ...https://jcm.asm.org/content/40/5/1603?ijkey=ef2d5a086cc059a7a56130f77fa5dc917aac8610&keytype2=tf_ipsecsha
YscP and YscU Regulate Substrate Specificity of the Yersinia Type III Secretion System | Journal of Bacteriology
Immunoblotting.Samples were separated by SDS-12% PAGE and electroblotted (Trans Blot SD; Bio-Rad) onto a nitrocellulose ...https://jb.asm.org/content/185/7/2259?ijkey=15839259dd3710b60317af9c3e10bbf1765bffb5&keytype2=tf_ipsecsha
Immunoblotting. Cells were lysed using Nonidet-40 lysis buffer (50 mm HEPES (pH 7.4), 250 mm NaCl, 5 mm EDTA, 1% Nonidet P-40, ... 2A-2C). Target protein expression was confirmed by immunoblotting for SH-tagged GFP (Figs. 2D-2F). Additionally, we observed ... and transgene expression in the target cell lines can be assessed by flow cytometry or immunoblotting prior to TAP-MS and ... were analyzed by immunoblotting with the indicated antibodies. Asterisks (**) denote SH-tagged RIPK3. Data shown are ...https://www.mcponline.org/content/15/3/1139
Anti-Survivin: Marker for blocking apoptosis | Sigma-Aldrich
Immunoblotting. Whole HeLa cell extracts treated with thymidine were separated on SDS-PAGE and blotted with Rabbit Anti- ...https://www.sigmaaldrich.com/technical-documents/articles/biology/anti-survivin.html
All Faculty | Duke Mechanical Engineering and Materials Science
Immunoblotting • Leukotriene B4 • Lipoxygenase • Nitric Oxide • Nitric Oxide Synthase • Nitric Oxide Synthase Type II • Rats • ...http://fds.duke.edu/db/pratt/mems/faculty/guilak/publications/106827
A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts |...
SDS-PAGE and immunoblotting. The wild-type and transplastomic C. reinhardtii cultures were grown in TAP or minimal media and ...https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-018-1127-7