Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens |...
The mAbs were screened by immunoflouorescence assays, ELISA and immunoblotting. Selected mAbs 5E5 and 8F10 were of isotypes IgM ...http://scholarbank.nus.edu.sg/handle/10635/24739
COX-2-Derived Prostacyclin Modulates Vascular Remodeling | Circulation Research
Immunoblotting for HO-1 was performed using a polyclonal antibody (Stressgen) and detected by a chemiluminescent method (ECL, ... Immunohistochemisty and Immunoblotting. Mice were exsanguinated via intracardial injection of PBS. Common carotid arteries were ...http://circres.ahajournals.org/content/96/12/1240
In Vitro Inhibition and Induction of Human Hepatic Cytochrome P450 Enzymes by Modafinil | Drug Metabolism & Disposition
Western Immunoblotting Procedures.. CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C19, and CYP3A4 in the hepatocyte-derived microsomes ... However, CYP2C8 protein was detected in all of the microsomal preparations by Western immunoblotting. The levels of the protein ... To determine whether the changes in enzyme activity were due to changes in enzyme concentration, Western immunoblotting was ... and a portion of each was subjected to Western immunoblotting to measure the levels of immunoreactive CYP3A4 using a polyclonal ...http://dmd.aspetjournals.org/content/28/6/664.long
Rapid electrical stimulation of contraction modulates gap junction protein in neonatal rat cultured cardiomyocytes | JACC:...
In the immunoblotting (Fig. 1A),the Cx43 antibody recognized three bands migrating between 40 and 43 kDa. The amount of Cx43 ... Electrophoresis and immunoblotting. Collected cells were lysed, and the cellular extracts were electrophoresed on SDS- ... 3A).Figure 3B shows the protein amount of AngII in cardiomyocytes estimated by immunoblotting. Angiotensin II was increased ... The amount of protein recognized by the antibodies was quantified by means of an ECL immunoblotting detection system (Amersham ...http://www.onlinejacc.org/content/44/4/914
VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction | Biochemical Journal
Immunoprecipitation and immunoblotting. Transfected HEK293T cells were harvested and lysed in 10 mM Tris-HCl (pH 7.5), 150 mM ... IRDye 800CW-conjugated secondary antibodies for immunoblotting were supplied by LI-COR: goat anti-mouse (cat. 925-32210), goat ... G1544). Three antibodies against VPS8 were tested, but failed our validation assays for immunoblotting: polyclonal anti-VPS8 ( ... The following primary antibodies were used for immunoblotting: monoclonal anti-myc (Millipore, cat. 05-724); polyclonal anti- ...http://www.biochemj.org/content/474/21/3615
Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway | Molecular...
... catenin immunoblotting. Actin levels were detected by immunoblotting as a loading control. (D, E) HEK293T cells were ... Immunoprecipitation and immunoblotting. HEK293T cells were transfected using calcium phosphate and immunoprecipitation (IP) and ... Interactions were detected by anti‐Flag immunoprecipitation followed by anti‐β‐catenin immunoblotting. (B) Ube2m overexpression ... A similar association of Nkd1‐HA with 3XFlag‐Axin1 was detected by immunoprecipitation followed by immunoblotting ( ...http://msb.embopress.org/content/5/1/315.long
Activation of Transient Receptor Potential Melastatin Subtype 8 Attenuates Cold‐Induced Hypertension Through Ameliorating...
Western Immunoblotting. Cells or tissues were lysed in a buffer containing 0.5 mol/L Tris, 1% NP40, 1% Triton X‐100, 1 g/L ...http://jaha.ahajournals.org/content/6/8/e005495
The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis |...
Protein Extraction, Immunoblotting, and Immunoprecipitation. Plant material was ground in liquid nitrogen. Total protein was ... we subjected microsomal fractions to proteinase K treatments followed by SDS-PAGE and immunoblotting. Whereas all five RGPs ... proteinase K before detergent solubilization and analyzed by SDS-PAGE and immunoblotting. ... of RGP-YFP fusion proteins expressed under the control of their native promoters were confirmed by immunoblotting and enzyme ...http://www.plantcell.org/content/23/4/1373.long
Western Blotting Products (Basic Research), Reviews & Suppliers | SelectScience
Anti-Cdc42 Immunoblotting Kit. MilliporeSigma, a business of Merck KGaA Darmstadt Germany. Anti-cdc42 is a mouse monoclonal ... immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab ...https://www.selectscience.net/western-blotting-equipment-
Histone H4 acetylation regulates behavioral inter-individual variability in zebrafish | Genome Biology | Full Text
Immunoprecipitation and western immunoblotting. Groups of 20 fish (control, NaBu-treated, hdac1 +/−, and yy1 +/−) were frozen ...https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1428-y
P19 Cells as a Model for Studying the Circadian Clock in Stem Cells before and after Cell Differentiation
Protein extraction and immunoblotting. Cell pellets intended for protein extraction were suspended in 100 µl T-PER tissue ... To further validate the results, a mouse neural stem cell marker, SSEA1, was also tested using immunoblotting. Figure 1E shows ... All gel electrophoresis for immunoblotting was performed using Bio-Rad systems and buffers according to the manufacturer's ...https://www.jcircadianrhythms.com/articles/10.5334/jcr.157/
Oncotarget | CoGAPS matrix factorization algorithm identifies transcriptional changes in AP-2alpha target genes in feedback...
Immunoblotting. Protein lysates were prepared as previously described . Lysed protein concentration was measured by the ...http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=12075&path%5B%5D=38216
Molecular investigation of the 7.2 kb RNA of murine cytomegalovirus | Virology Journal | Full Text
Immunoblotting. Cells were trypsinized, centrifuged, and collected in PBS. Cells were lysed in RIPA buffer (150 mM NaCl, 1% v/v ... Immunoblotting for the m106-GFP fusion protein with antibody specific for GFP only detected protein expression during MCMV: ...https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-10-348
Interferon-γ Activates Expression of p15 and p16 Regardless of 9p21.3 Coronary Artery Disease Risk Genotype | JACC: Journal of...
Processing of cells for immunoblotting. Cells were lysed in RIPA buffer (4.25 mmol/l Tris [pH 8.0], 135 mmol/l NaCl, 1% IGEPAL ...http://www.onlinejacc.org/content/61/2/143
Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway...
b An immunoblotting assay revealed an evoked expression of RAS, RAF, p-RAF, MEK, p-MEK, ERK and p-ERK proteins in cells ... Immunoblotting analysis. Whole cell extracts were prepared by homogenizing cells in lysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM ... The release of Cyt-C is another hallmark of mitochondrial dysfunction and cell apoptosis , immunoblotting analysis also ... an immunoblotting assay was employed to analyse the RAS, RAF, phosphorylated-RAF (p-RAF), MEK, p-MEK, ERK and p-ERK proteins in ...https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0842-0
Protective effect of Rhei Rhizoma on reflux esophagitis in rats via Nrf2-mediated inhibition of NF-κB signaling pathway | BMC...
Immunoblotting analyses. For the estimation of Nrf2, NF-κBp65, and histone, 10 μg of protein from each nuclear fraction was ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0974-z
Anti-Ganglioside GM₁ Rabbit pAb | 345757
ELISA and TLC immunoblotting. - Find MSDS or SDS, a COA, data sheets and more information. ... Immunoblotting (Western Blotting). Application Notes. ELISA (see comments). TLC Immunoblotting (1:1000, colormetric). ... ELISA and TLC immunoblotting. Less,, Anti-Ganglioside GM₁ Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, ... ELISA and TLC immunoblotting.. More,, Anti-Ganglioside GM₁, rabbit polyclonal, recognizes ganglioside GM₁. Cross-reacts ...http://www.emdmillipore.com/PR/en/product/Anti-Ganglioside-GM1-Rabbit-pAb,EMD_BIO-345757
The Antibacterial Activity of Human Neutrophils and Eosinophils Requires Proton Channels but Not BK Channels | JGP
Protein Electrophoresis and Immunoblotting. Samples were resolved by 10% SDS-PAGE (PAGE) and transferred to nitrocellulose. ... If the level of BK channel expression were very low, protein might be functional yet undetectable by immunoblotting. However, a ... and heat denatured for immunoblotting at 100°C for 3 min. ...http://jgp.rupress.org/content/127/6/659
In vivo administration of quercetin ameliorates sperm oxidative stress, inflammation, preserves sperm morphology and functions...
Immunoblotting analyses of sperm inflammatory markers Sperm were disrupted using a rotor-stator homogenizer (Heidolph DIAX 600 ...https://www.termedia.pl/In-vivo-administration-of-quercetin-ameliorates-sperm-oxidative-stress-inflammation-preserves-sperm-morphology-and-functions-in-streptozotocin-nicotinamide-induced-adult-male-diabetic-rats,19,34571,1,1.html
Immunoblotting. Immunoblotting was performed essentially as described previously . Cell lysates with 1% NP-40, 0.1% SDS, 1 ... For Immunoblotting, mouse monoclonal antibodies against GAPDH (6C5; Santa Cruz Biotechnology, Santa Cruz, CA, USA), α-tubulin ( ... Expression of liver markers was measured by qRT-PCR (D) and Immunoblotting (E). Data were normalized to the expression of GAPDH ... and immunoblotting (C). Data were normalized to the expression of GAPDH mRNA. D. Expression of cell surface CD81 was examined ...http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=19108&path%5B%5D=61229
Instructions 2025 NHSN Rev. 1.6 (11/06)
Immunoblotting. The basic procedure for gold immunoblotting has been described by Moeremans et al,11 which may be followed. For ... Immunoblotting *References Guide to Gold Cluster Labeling: Our detailed description of gold cluster labeling, how to isolate ... Silver enhancement can be used to make the immunolabeling useful for light microscopy and immunoblotting with improved results ... NANOGOLD® conjugates can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. They ...http://nanoprobes.com/instructions/In2025S.html
Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits | Virology Journal |...
Immunoblotting. Sf9 insect cells were infected with 3 pfu/cell of each baculovirus individually, or co-infected with pairwise ... After 48 h, soluble cytoplasmic and nuclear fractions were prepared and analyzed by immunoblotting. UL56 and UL89 were detected ...https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-315
The anti-adiposity effect of bitter melon seed oil is solely attributed to its fatty acid components | Lipids in Health and...
Immunoblotting. Inguinal fat was homogenized in RIPA buffer containing 1% protease inhibitor cocktail and 1% phosphatase ...https://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0578-3
Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model | Critical Care ...
Immunoblotting in lung tissue homogenates. NOS, 3-NT, PAR, p65, and IL-8 protein expressions were measured using a western blot ...https://ccforum.biomedcentral.com/articles/10.1186/cc9097
Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas - 1st Edition
Immunoblotting Tissue Staining Embedding Immunostaining Protocol for Tissue. Results Discussion. References 13 Distinction ...https://www.elsevier.com/books/handbook-of-immunohistochemistry-and-in-situ-hybridization-of-human-carcinomas/hayat/978-0-12-088404-9