Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting |...
Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting. CLODAGH ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ... Analysis of Candida albicans antigens by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and immunoblotting ...http://www.biochemsoctrans.org/content/14/2/435
BCL-3 expression promotes colorectal tumorigenesis through activation of AKT signalling | Gut
Immunoblotting. Whole-cell lysates were prepared and subjected to immunoblotting as previously described38 using antibodies ...https://gut.bmj.com/content/65/7/1151
MicroRNA miR145 Regulates TGFBR2 Expression and Matrix Synthesis in Vascular Smooth Muscle CellsNovelty and Significance |...
Immunoblotting. Equivalent amounts of protein were run on 10% SDS-PAGE gels, transferred to nitrocellulose membranes (Millipore ...http://circres.ahajournals.org/content/116/1/23
Rapidly progressive dementia with thalamic degeneration and peculiar cortical prion protein immunoreactivity, but absence of...
Immunoblotting. Analysis of brain samples by Western blotting, including centrifugal concentration. For Western blot analysis, ... in conjunction with PrPres that is detectable by immunoblotting. Thus, in PrDs the disease-associated PrPSc may be in the form ... Immunoblotting shows a ladder-like pattern of protease-resistant PrP fragments, extending from approximately 8 kDa to the 18-30 ... In human PrDs three major PK-resistant PrP types have been described that are readily identifiable by immunoblotting with a ...https://actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-1-72
Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction | eLife
Immunoblotting. Request a detailed protocol The immunoblot in Figure 3F was prepared as follows: cell ghost pellet and ...https://elifesciences.org/articles/21383
Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers | eLife
Immunoblotting. Request a detailed protocol Nuclear fractions were prepared by incubation on ice in buffer A (25 mM HEPES, pH ... Twenty 0.5 ml fractions were then collected for immunoblotting analysis. ...https://elifesciences.org/articles/30506
Anti-tau conformational scFv MC1 antibody efficiently reduces pathological tau species in adult JNPL3 mice | Acta...
Immunoblotting. An aliquot of the total lysates was used for immunoblotting. 0.1% SDS was added to the lysates, followed by ... Immunoblotting analysis was performed in order to confirm the scFv expression in the hippocampi: as shown in Fig. 2b, scfv-MC1 ... In the cell lysate (immunoblotting) the scFv-MC1 was detected as a 30 kDa band; c in the conditioned medium the presence of ... Of note, scFv-MC1 was not detected in serum, by neither immunoblotting or ELISA (data not shown), as already reported by others ...https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0585-2
G‐quadruplex‐binding small molecules ameliorate C9orf72 FTD/ALS pathology in vitro and in vivo | EMBO Molecular Medicine
Immunoblotting. Fly abdomens were homogenised in 20 μl per abdomen of 2× Laemmli sample buffer (4% SDS, 20% glycerol, 120 mM ... After 7 days, flies were snap‐frozen in liquid nitrogen for analysis of abdominal poly(GP) levels by immunoblotting. ...http://embomolmed.embopress.org/content/10/1/22
Circadian Pattern of Melatonin MT1 and MT2 Receptor Localization in the Rat Suprachiasmatic Nucleus
For immunoblotting, 4 rats were decapitated rapidly after 1 minute of exposure to CO2 at dusk (ZT 11-13). The brains were ... Immunoblotting of rat hypothalamus (HT) with MT1 and MT2 receptors antibodies. A) Immunoblot of rat hypothalamic tissue with ... Immunoblotting. Immunoblots were performed under standard conditions with the same antibodies used for immunohistochemistry. A ...https://www.jcircadianrhythms.com/articles/10.5334/jcr.ab/
Altered Expression of Major Renal Na Transporters in Rats with Unilateral Ureteral Obstruction - NDI Foundation
0.17 +/- 0.03 micromol/min, P < 0.05). Consistent with this, immunoblotting revealed significant reductions in the abundance of ... 0.05). Consistent with this, immunoblotting revealed significant reductions in the abundance of major renal Na transporters: ...http://www.ndif.org/articles/45-Altered_Expression_of_Major_Renal_Na_Transporters_in_Rats_with_Unilateral_Ureteral_Obstruction/body
Nephrin Tyrosine Phosphorylation Is Required to Stabilize and Restore Podocyte Foot Process Architecture | American Society of...
Immunoblotting. Protein samples were resolved on 8% or 12% SDS-PAGE gels with 100 μg protein loaded as total lysate. Proteins ... B) Nephrin protein expression and phosphorylation was evaluated using immunoblotting (IB) for the indicated antibodies on ... Secondary antibodies for immunoblotting from Bio-Rad (Hercules, CA) were goat anti-mouse HRP (170-6516) and goat anti-rabbit ... were first conjugated to Nck antibodies to avoid interference from IgG heavy chain during immunoblotting as described elsewhere ...https://jasn.asnjournals.org/content/27/8/2422
Immunoblotting.. In experiments assessing total levels of proteins, C6 cells or Cav-1 RNAi cells were placed in lysis buffer ( ... Equal protein (5 μg) of BF and HF were analyzed by immunoblotting (Fig. 2C). This equal protein comparison showed that Gαs ... Gαs-GFP expression was compared with endogenous Gαs expression using immunoblotting and was approximately 3-fold higher than ... Total protein lysates from wild-type C6 or stable caveolin-1 knockdown cells were analyzed by SDS/PAGE and immunoblotting to ...http://molpharm.aspetjournals.org/content/76/5/1082
A gp91phox Containing NADPH Oxidase Selectively Expressed in Endothelial Cells Is a Major Source of Oxygen Radical Generation...
Immunoblotting. Western blot analysis was performed as described previously.14 p22phox SDS-PAGE was performed using a membrane ...http://circres.ahajournals.org/content/87/1/26
Loss of the mitochondrial i‐AAA protease YME1L leads to ocular dysfunction and spinal axonopathy | EMBO Molecular Medicine
Immunoblotting. Tissues were homogenized in ice‐cold RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X‐100, 0.1% ... old mice by immunoblotting and quantitative real‐time PCR. The autophagy marker proteins p62 and LC3B did not accumulate at ... and immunoblotting using the following antibodies: rabbit anti‐GFAP (Z0334, Dako, 1:1,000), mouse anti‐GAPDH (sc‐32233, Santa ...http://embomolmed.embopress.org/content/early/2018/11/02/emmm.201809288.share
Rab22A recruits BLOC‐1 and BLOC‐2 to promote the biogenesis of recycling endosomes | EMBO Reports
Immunoblotting. Protein levels in the cell lysate were analysed by immunoblotting as described previously . Cells were ... C, D Quantification of melanin content (C, n = 5) and the protein levels of TYRP1 (judged by immunoblotting in D, n = 3) in ... D. Immunoblotting analysis of proteins in control and Rab22A‐, BLOC‐1‐, BLOC‐2‐depleted HeLa cells. *, non‐specific bands. ... Immunoblotting analysis of Tf receptor in HeLa cells as indicated. Protein band intensities were quantified and indicated on ...http://embor.embopress.org/content/19/12/e45918
Flavanoids induce expression of the suppressor of cytokine signalling 3 (SOCS3) gene and suppress IL-6-activated signal...
Immunoblotting. Cell lysates were prepared in sample buffer [50 mM Tris/HCl (pH 6.8), 2% (w/v) SDS, 10% (v/v) glycerol, 1% (v/v ... Stimulation of HUVECs with flavone was found to elicit a degree of AMPK activation, as determined by immunoblotting cell ... Tyrosine phosphorylation of STAT3 was determined by immunoblotting cell extracts with anti-STAT3 and anti-phospho-STAT3 (Tyr705 ... Jolanta Wiejak and Julia Dunlop performed the majority of the experimental work, including immunoblotting, RT-PCR, luciferase ...http://www.biochemj.org/content/454/2/283.full
BCL-3 expression promotes colorectal tumorigenesis through activation of AKT signalling | Gut
Immunoblotting. Whole-cell lysates were prepared and subjected to immunoblotting as previously described38 using antibodies ...http://gut.bmj.com/content/65/7/1151
Targeting beta-Catenin signaling to induce apoptosis in human breast cancer cells by z-Guggulsterone and Gugulipid extract of...
Immunoblotting for TCF-4 using β-Catenin immunoprecipitates from MCF-7 (H) and MDA-MB-231 (G) cells treated for 24 h with DMSO ... Immunoblotting was performed as described by us previously[26-28]. The blots were stripped and re-probed with the anti-actin ... Immunoblotting for β-Catenin protein using isolated cytosolic and nuclear fractions from MCF-7 (C) or MDA-MB-231 (D) cells ... Immunoblotting for TCF-4 using lysates from MCF-7 (A) or MDA-MB-231 (B) cells treated with DMSO (control) or 5 μmol/L GL for 24 ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-203
ABCC5 supports osteoclast formation and promotes breast cancer metastasis to bone | Breast Cancer Research | Full Text
Immunoblotting. Human and mouse cell lines were lysed in TNE lysis buffer, as previously described . Total protein ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3361
The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive...
Immunoblotting. For immunoblotting, the whole cell lysates were prepared as described previously . Detailed information on ... the primary antibodies used in immunoblotting analysis are listed in Additional file 6: Table S5. The signals were quantified ...https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1412-6