D) Phosphorylation dynamics and respective expression levels of proteins analyzed in C were monitored by immunoblotting. ... Protein loading was monitored by anti-actin immunoblotting (Lower). (D) ARFGEF2 and ACTN4 shRNAs are toxic for BL. The figure ... Protein loading was monitored by anti-actin immunoblotting (Lower). ARFGEF2 and ACTN4 shRNAs are toxic for BL. The figure shows ...

Immunoblotting were performed using anti-COP1 (57), anti-PIF3 (58), anti-HY5 (10), anti-GFP (Abmart), anti-CSN6 (59), anti-myc ... Immunoblotting was performed using anti-COP1, anti-histone 3, and anti-tubulin antibodies. (G) Immunoblot analysis showing the ... His-TF and His-TF-COP1 proteins were detected by immunoblotting using anti-His antibodies. GST and GST-PID proteins were ... Bound and 10% of input His-PID fractions were detected by immunoblotting using anti-His antibodies. Immobilized GST and GST- ...

FLWT, KUWT, and KDWT were analyzed by (A) anti-pS172 immunoblotting and (B) LC-MS before and after incubation with ATP. ... KUWT was evaluated by pS172 immunoblotting. The KUS172A-mediated transphosphorylation of KUD135N is observable at longer time ... active TBK1 constructs were added to the purified D135N mutants and pS172 status was monitored by immunoblotting (Fig. 3C). The ... as evaluated by phospho-specific immunoblotting and LC-MS, gave similar results (Fig. S8). ...