Glycogen Phosphorylase Isoenzyme BB Plasma Concentration Is Elevated in Pregnancy and Preterm Preeclampsia | Hypertension
When we conducted immunoblotting using the placental protein lysates of 45 cases (Table S1, available in the online Data ... Immunoblotting. Villous placental tissue samples were pooled from 5 different sampling sites. An equal amount of placental ... Densitometric analysis of immunoblotting data showed a significant decrease in the expression of GPBB in preterm preeclampsia ... GPBB was readily detected in the villous placenta of all cases with immunoblotting. B, Placental GPBB was less abundant in ...http://hyper.ahajournals.org/content/59/2/274
Angiotensin II Regulation of AT1 and D3 Dopamine Receptors in Renal Proximal Tubule Cells of SHR | Hypertension
Immunoblotting. The antibodies are polyclonal purified antipeptides. The amino acid sequence for the immunogenic peptide ( ... Cell lysate protein (100 μg) was subjected to immunoblotting with anti-AT1 receptor antibody (1:400). The 45-kDa band was ... The pellets were suspended in sample buffer, boiled for 10 minutes, and subjected to immunoblotting with the AT1 receptor ... The supernatants were stored at −70°C until use for immunoblotting and/or immunoprecipitation. ...http://hyper.ahajournals.org/content/41/3/724
PI3-Kinase-Induced Hyperreactivity in DOCA-Salt Hypertension Is Independent of GSK-3 Activity | Hypertension
Immunoblotting. Aortae and carotid arteries were cleaned and pulverized in liquid nitrogen and solubilized in lysis buffer and ... prepared as previously reported.14 Whole cell lysates were separated by SDS-PAGE and immunoblotting was performed as previously ...http://hyper.ahajournals.org/content/41/4/898
Aberrant D1 and D3 Dopamine Receptor Transregulation in Hypertension | Hypertension
Immunoblotting. Rat RPT cells and CASMCs were treated with vehicle (dH2O), a D1-like receptor agonist (fenoldopam), or a D1- ... Immunoblotting was performed as previously reported, except that the transblots were probed with the D1 (1:800) or the D3 ... All immunoblotting results are expressed as relative density units (DU) (n=10; *P,0.05 versus control; ANOVA, Duncan test). C, ... The pellets were suspended in sample buffer, boiled for 10 minutes, and subjected to immunoblotting with the D3 receptor ...http://hyper.ahajournals.org/content/43/3/654
Internal Pudental Artery Dysfunction in Diabetes Mellitus Is Mediated by NOX1-Derived ROS-, Nrf2-, and Rho Kinase-Dependent...
Immunoblotting. Quiescent VSMC were stimulated with HG and proteins extracted, separated by electrophoresis on a polyacrylamide ...http://hyper.ahajournals.org/content/68/4/1056
Prostaglandin E-Prostanoid4 Receptor Mediates Angiotensin II-Induced (Pro)Renin Receptor Expression in the Rat Renal...
By immunoblotting, the full-length PRR was detected as a 43-kDa band. Exposure to 1 μmol/L Ang II for 12 hours elevated the ... Immunoblotting. Renal tissues were lysed and subsequently sonicated in PBS that contained 1% Triton X-100, 250 μmol/L ... Shown is the densitometric result from PRR immunoblotting. B, Effects of EP antagonists on PRR mRNA expression during Ang II ... The expression was determined by using immunoblotting analysis (n=5 per group). Data are mean±SE. CTR indicates control. ...http://hyper.ahajournals.org/content/64/2/369
Adenovirus-Mediated Overexpression of Caveolin-3 Inhibits Rat Cardiomyocyte Hypertrophy | Hypertension
Immunoblotting. Cell lysates were separated by electrophoresis through a 10% to 12% polyacrylamide gel and transferred to a ... Immunoblotting was performed with anti-polyclonal βMHC. In Ad.LacZ-infected cardiomyocytes, expression of βMHC was ...http://hyper.ahajournals.org/content/42/2/213
Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Mediated Redox Signaling and Vascular Remodeling by 16α-Hydroxyestrone in...
Immunoblotting. Immunoblotting was used to examine protein expression of proliferating cell nuclear antigen, p27, Nox1 and Nox4 ... were determined by immunoblotting. Results are expressed as mean±SEM of 5 experiments where protein expression is relative to β ...http://hyper.ahajournals.org/content/68/3/796
cGMP-Dependent Activation of Protein Kinase G Precludes Disulfide ActivationNovelty and Significance | Hypertension
Immunoblotting. Proteins were resolved using SDS-PAGE, Western blotted, and then immunostained using antibodies to PKG1, PKARI ... and MnSOD compared with aorta determined using immunoblotting (n=4-5). D, 2-Cys peroxiredoxin becomes hyperoxidized to a ...http://hyper.ahajournals.org/content/60/5/1301
Endothelin-1-Induced Enhancement of Coronary Smooth Muscle Contraction via MAPK-Dependent and MAPK-Independent [Ca2+]i...
Immunoblotting. Samples of the immunoprecipitate were subjected to electrophoresis on 8% SDS polyacrylamide gels then ...http://hyper.ahajournals.org/content/39/2/543
Cardiac βARK1 Upregulation Induced by Chronic Salt Deprivation in Rats | Hypertension
Protein Immunoblotting. Immunodetection of myocardial levels of βARK1 was performed on detergent-solubilized extracts after ... LV βARK1 expression was assessed by protein immunoblotting and βARK1 activity by in vitro GRK phosphorylation assays. We ...http://hyper.ahajournals.org/content/38/2/255
Gestational Hypoxia Increases Reactive Oxygen Species and Inhibits Steroid Hormone-Mediated Upregulation of Ca2+-Activated K+...
Western Immunoblotting. Our previous study demonstrated that pregnancy increased protein abundance of type 2 (SK2) and type 3 ( ...http://hyper.ahajournals.org/content/64/2/415
PPARγ-Activating Angiotensin Type-1 Receptor Blockers Induce Adiponectin | Hypertension
Western Immunoblotting. After stimulation with the indicated agents, protein isolation, electrophoresis, and blotting were ... At the indicated time, steady-state levels of adiponectin were determined by immunoblotting (Figure 3A). In vehicle treated- ... Adiponectin protein expression was measured by Western immunoblotting as described in Methods. Protein expression is shown ... Adiponectin protein expression was measured by Western immunoblotting as described in Methods. Protein expression is shown ...http://hyper.ahajournals.org/content/46/1/137
Effects of Angiotensin II Type 1 Receptor Blockade and Angiotensin-Converting Enzyme Inhibition on Cardiac β-Adrenergic Signal...
Immunoblotting Techniques. Immunoblotting techniques were performed as described previously.14 The polyclonal antiserum (MB 1) ...http://hyper.ahajournals.org/content/31/3/747
Regulation of Na+,K+-ATPase α-Subunit Expression by Mechanical Strain in Aortic Smooth Muscle Cells | Hypertension
Gel Electrophoresis and Immunoblotting We loaded 5 and 15 μg of the cell extract protein for α1- and α2-isoforms, respectively ...http://hyper.ahajournals.org/content/27/3/827
CD36 and Na/K-ATPase-α1 Form a Proinflammatory Signaling Loop in KidneyNovelty and Significance | Hypertension
Reagents, Cell Culture, and Immunoblotting. Tissue culture media, supplements, reagents, and immunoblotting material and ...http://hyper.ahajournals.org/content/61/1/216.full
Impaired Prostaglandin E2/Prostaglandin I2 Receptor-Gs Protein Interactions in Isolated Renal Resistance Arterioles of...
Immunoblotting of α Subunits of G Protein. Solubilized protein (15 to 30 μg, given according to the linearity of protein loaded ... Immunoblotting techniques were used to determine the existence and relative amounts of G proteins. Western blots demonstrated ...http://hyper.ahajournals.org/content/34/5/1134
Wine Polyphenols Decrease Blood Pressure, Improve NO Vasodilatation, and Induce Gene Expression | Hypertension
Immunoblotting With Anti-iNOS and Anti-Inducible COX-2 Antibodies. Aortas were homogenized, and ≈100 μg of total protein from ...http://hyper.ahajournals.org/content/38/2/159
Angiotensin-Converting Enzyme 2 Metabolizes and Partially Inactivates Pyr-Apelin-13 and Apelin-17Novelty and Significance |...
Total protein extraction and immunoblotting were performed as previously described.8 Left ventricle tissue was homogenized ... and subjected to immunoblotting of phospho-(Ser473)/total Akt, phospho-(Thr308)/total Akt, and phospho/total Erk1/2 (Cell ...http://hyper.ahajournals.org/content/68/2/365
Abstract 81: Activation of Microglia in the PVN Precedes Increase in Blood Pressure in Chronic Angiotensin II Infusion Rat...
CD11b immunoblotting of the PVN was used to assess the activated microglia in the PVN. Additionally, Iba1 immunohistochemistry ...http://hyper.ahajournals.org/content/60/Suppl_1/A81
Imatinib Mesylate Attenuates Myocardial Remodeling Through Inhibition of Platelet-Derived Growth Factor and Transforming Growth...
Their hearts were extracted for histopathologic, immunoblotting, and quantitative reverse transcriptase polymerase chain ...http://hyper.ahajournals.org/content/early/2014/03/03/HYPERTENSIONAHA.113.01866
Smad3 Couples Pak1 With the Antihypertrophic Pathway Through the E3 Ubiquitin Ligase, Fbxo32Novelty and Significance |...
D, Immunoblotting shows that Smad3 phosphorylation is abrogated in Pak1cKO(cKO)/transverse aortic constriction (TAC) mice. E, ...http://hyper.ahajournals.org/content/66/6/1176
Variants in Striatin Gene Are Associated With Salt-Sensitive Blood Pressure in Mice and HumansNovelty and Significance |...
Immunoblotting analysis of Strn+/− mouse tissues (adrenal, heart, and kidney) confirmed that targeted allele effectively ...http://hyper.ahajournals.org/content/65/1/211
Glucocorticoid-Related Signaling Effects in Vascular Smooth Muscle Cells | Hypertension
Spironolactone prevented corticosterone-induced ERK1/2 phosphorylation at 15 minutes, as shown by immunoblotting (Figure S3A) ...http://hyper.ahajournals.org/content/51/5/1372
Tissue Kallikrein Actions at the Rabbit Natural or Recombinant Kinin B2 Receptors | Hypertension
... same antibodies used as in the enzyme immunoassay applied to immunoblotting; data not shown). The assay was not sensitive ... is in a concentration range that does not promote detectable digestion of the fusion protein as assessed by immunoblotting ( ...http://hyper.ahajournals.org/content/41/3/611