Cell Fractionation of Pseudomonas aeruginosa -BIO-PROTOCOL
The proteins can then be visualized by SDS-PAGE or Immunoblotting (Cooksley et al., 2003) as seen in Figure 1. It is good ... followed by immunoblotting with specific anti-AaaA sera as described previously (Luckett et al., 2012). Whole cell (W), ...https://bio-protocol.org/e922
A Method for SUMO Modification of Proteins in vitro
SUMO modification is most commonly detected by immunoblotting. Like other post-translational modifications such as ... followed by immunoblotting for the protein of interest. Furthermore, recent advances in proteomic methods have made the ... followed by Ni-NTA purification under denaturing conditions and immunoblotting for co-purifying proteins of interest (Lee et al ...https://bio-protocol.org/e3033
The RiboPuromycylation Method (RPM): an Immunofluorescence ...
... to detect puromycylated nascent chains released from ribosomes by immunoblotting and immunoprecipitation. Subsequently, ...https://bio-protocol.org/cn/e2669
PNGase Sensitivity Assay to Study the Folding Status of ...
Separate 10 µl of the samples by SDS-PAGE followed by immunoblotting using PVDF membrane and probing with an antibody against ... SDS-PAGE followed by immunoblotting with antibodies against the myc-tag. The data shown represents a single representative ...https://bio-protocol.org/e1952
In vitro Chitin Binding Assay -BIO-PROTOCOL
... immunoblotting (see Figure 1). ...https://bio-protocol.org/e1401
Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins -BIO-PROTOCOL
Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins Edited by. Fanglian He ... How to cite: Lehti, T. A. and Westerlund-Wikström, B. (2013). Colony Immunoblotting Assay for Detection of Bacterial Cell- ... Lehti, T. A. and Westerlund-Wikström, B. (2013). Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or ... Figure 2. A representative colony immunoblotting membrane of Mat fimbriae expression in Escherichia coli MG1655-Rif derivatives ...http://www.bio-protocol.org/e888
Nuclear/Cytoplasmic Fractionation of Proteins from ...
... which may be useful during immunoblotting. However, in this situation the signal cannot be compared directly to that of the ...https://bio-protocol.org/e3053
Probing Conformational States of a Target Protein in ...
SDS-PAGE and immunoblotting * Assemble a 10% SDS-PAGE gel in a Mini-PROTEAN® Tetra system.. Note: The % of SDS-PAGE gel should ... This should be reflected by the absence of the band corresponding to full-length protein after immunoblotting (Figures 5C and ... An N-terminal FLAG tag used for detection by immunoblotting is shown as a red flag on the N lobe. In the absence of thrombin ... for detection by immunoblotting (Figure 2B). Using this protocol, we demonstrated that, in vivo, the glycolipid MurJ ...https://bio-protocol.org/e3271
Expression and Partial Purification of His-tagged Proteins ...
At this stage protein crude extracts can be tested for the presence of the protein of interest using immunoblotting techniques ... Alternatively flow through of the washes can be kept to monitor the presence of the protein of interest using immunoblotting ...https://bio-protocol.org/e1572