HT29 Cells - Semantic Scholar
... cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS. ... HT29 Cells. Known as: Cells, HT-29, HT-29, Cell, HT29 (More). ... Cell Culture Techniques. Cultured Cells. Tumor Cells, Cultured ... Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. ... Characteristics of apical chloride channels in human colon cells (HT29). *John P. Hayslett, Heinz Goegelein, Karl Kunzelmann, ...https://www.semanticscholar.org/topic/HT29-Cells/12259
Benzyl isothiocyanate (BITC) inhibits migration and invasion of human colon cancer HT29 cells by inhibiting matrix...
... levels of MMP-2 were observed in BITC-treated HT29 cells. BITC also inhibited mRNA levels of MMP-2, -7, and -9 in HT29 cells. ... In this study, we show for the first time the antimetastatic effects of BITC in human colon cancer HT29 cells. BITC had an ... Moreover, BITC decreased the levels of c-Fos, c-Jun, Ras, FAK, PI3K and GRB2 in HT29 cells. Reductions in the enzyme activity, ... These results demonstrated that BITC could alter HT29 cell metastasis by reduction of MMP-2, MMP-9, and u-PA expression through ...https://www.ncbi.nlm.nih.gov/pubmed/20136087?dopt=Abstract
AID 301247 - Agonist activity at PAR2 expressed in human HT29 cells assessed as intracellular calcium release - PubChem
Agonist activity at PAR2 expressed in human HT29 cells assessed as intracellular calcium release. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/301247
AID 320856 - Inhibition of human Cdc2/cyclin B-mediated histone H1 kinase phosphorylation in HT29 cells - PubChem
Inhibition of human Cdc2/cyclin B-mediated histone H1 kinase phosphorylation in HT29 cells. ...https://pubchem.ncbi.nlm.nih.gov/bioassay/320856
Antitumor 1-nitroacridine derivative C-1748, induces apoptosis, necrosis or senescence in human colon carcinoma HCT8 and HT29...
Here, we elucidated the nature of cellular response of human colon carcinoma HCT8 and HT29 cells to C-1748 treatment, at ... Compared with HCT8 cells, the fraction of sub-G1 HT29 cells did not exceed 14%, even following 4-fold dose escalation. ... Here, we elucidated the nature of cellular response of human colon carcinoma HCT8 and HT29 cells to C-1748 treatment, at ... On the contrary, no beta-galactosidase staining was observed in HT29 cells after C-1748 treatment. Moreover, prolonged drug ...http://www.biomedsearch.com/nih/Antitumor-1-nitroacridine-derivative-C/20026080.html
Hiperfect siRNA in HT-29 cells help - siRNA, microRNA and RNAi - BioForum
I'm seeding on 24 well plates at 180,000 cells/well. I was going to start again with 35mM and 50mM and 9ul hiperfect at 48h and ... Does anyone have experience in using hiperfect transfection reagent in HT-29 cells? So for I've used 5, 10, and 25mM siRNA with ... Hiperfect siRNA in HT-29 cells help - posted in siRNA, microRNA and RNAi: ... It works really well in DLD cells - knocks the gene down by ~96%. There are about 4 times as many HT29 cells in one well than ...http://www.protocol-online.org/forums/topic/27294-hiperfect-sirna-in-ht-29-cells-help/
HT29 colorectal cells, nucleus stain FITC (green) - Biology Forum | Biology-Online Dictionary, Blog & Forum
HT29 colorectal cells, nucleus stain FITC (green). Discussion of all aspects of cellular structure, physiology and ... hey.. i need some solutions... why is my cell nucleus stain green as in the FITC should not it only stain the cell membrane? ...https://www.biology-online.org/biology-forum/viewtopic.php?t=2391
Quantitative Proteomics Profiling of RKO and HT29 Cell Lines
The colorectal cancer cell lines RKO and HT29 had been identified as the hepatic metastasis model in vitro. ... Quantitative Proteomics Profiling of RKO and HT29 Cell Lines by iTRAQ Coupled LC-MS/MS [ Vol. 15 , Issue. 3 ] ... Method: Here, we compared the two cell proteome profiles and found some new molecular events of CRC metastasis. Using iTRAQ ... out of which 212 were found to be significantly changed in two cell lines. 28 candidate genes were confirmed by Q-PCR and 9 ...http://www.currentproteomics.com/articles/160041/quantitative-proteomics-profiling-of-rko-and-ht29-cell-lines-by-itraq-coupled-lc-msms
Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen...
... colon adenocarcinoma cells (HT-29), non-small-cell lung cancer cells (A549), and normal hepatic cells (WRL-68). The ... The percentage of cell viability was calculated by using the cell viability of IFN-γ/LPS-induced group as the control,. ... The level of this enzyme is higher in treated HT-29 cells as compared to nontreated cells; this concludes that CLG induces cell ... Human hepatocellular carcinoma cells (HepG2), colon adenocarcinoma cells (HT-29), and normal hepatic cells (WRL-68) were ...https://www.hindawi.com/journals/ecam/2012/490136/
Insulin Regulates Cleavage of Procaspase-9 via Binding of X Chromosome-Linked Inhibitor of Apoptosis Protein in HT-29 Cells |...
Cell Culture and Chemical Treatment.. Human colon epithelial adenocarcinoma cell line HT-29 (generously provided by the Peter ... Janes et al. (43) emphasized that in HT-29 cells treated with TNF-α, Akt was the only kinase significantly activated over a ... Insulin Regulates Cleavage of Procaspase-9 via Binding of X Chromosome-Linked Inhibitor of Apoptosis Protein in HT-29 Cells. Ji ... C. Caspase-9 and XIAP were detected by Western blotting after immunoprecipitation of procaspase-9 or XIAP from the HT-29 cells ...http://cancerres.aacrjournals.org/content/64/24/9070
Anti-oxidative and Anti-cancer Activities of Treculia africana Extract in Human Colon Adenocarcinoma HT29 Cells | Korea Science
Anti-oxidative and Anti-cancer Activities of Treculia africana Extract in Human Colon Adenocarcinoma HT29 Cells - Anti-cancer; ... META treatment induced apoptosis of HT29 cells, showing an increase in the percentage of both SubG1 cells and Annexin V- ... META-treated HT29 cells also showed the release of cytochrome c from the mitochondria into the cytosol, activation of caspase-3 ... Anti-oxidative and Anti-cancer Activities of Treculia africana Extract in Human Colon Adenocarcinoma HT29 Cells. Oh, You Na; ...http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=SMGHBM_2015_v25n5_515
Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for...
... biological response modifiers and the family of cell adhesion-promoting molecules. ... Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for ... Antonio Brú, Juan-Carlos Souto, Sonia Alcolea, et al., "Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and ...https://www.hindawi.com/journals/mi/2009/817498/cta/
P105 Inhibition of cytokine release from HT-29 cells and ulcerative colitis biopsies is potentiated by combination of selective...
Compounds were assessed for their ability to inhibit IL-8 secretion from IL-1β stimulated HT-29 cells, an epithelial cell line ... TOP1210 mimics the inhibitory effect of combined selective kinase inhibitors in HT-29 cells and UC biopsies. These data ... P105 Inhibition of cytokine release from HT-29 cells and ulcerative colitis biopsies is potentiated by combination of selective ... P105 Inhibition of cytokine release from HT-29 cells and ulcerative colitis biopsies is potentiated by combination of selective ...https://www.ecco-ibd.eu/publications/congress-abstract-s/abstracts-2016/item/p105-inhibition-of-cytokine-release-from-ht-29-cells-and-ulcerative-colitis-biopsies-is-potentiated-by-combination-of-selective-kinase-inhibitors-and-such-effects-are-mimicked-by-top1210-a-narrow-spectrum-kinase-inhibitor.html
AddexBio Product Detail - HT-29 Cells
DLD-1 cells HCA-7 cells HCT-116 cells HCT-8 cells HCT-15 cells HT-29 cells LoVo cells LS-180 cells LS-174T cells RKO cells SNU- ... SW1116 cells SW48 cells SW480 cells SW620 cells T84 cells References for HT-29 cells:. 1. Brandenburg, L. O., Seyferth, S., ... Related cells by Organ:. Caco-2 cells CMT-93 cells COLO 205 cells ... HT-29 cells Catalog #:. C0009004 Shipping advice:. Use only Express Delivery (overnight) for cell lines. International shipping ...https://addexbio.com/productdetail?pid=48
Inhibition of CD147 expression by RNA interference reduces proliferation, invasion and increases chemosensitivity in cancer...
... cells in HT-29 cell line was ~47.0%. As described previously (27), several thousand GFP+ cells arising from HT-29 cells were ... Transfection of cells and generation of stable cell clones. HT-29 cells were plated in 6-well plates at a density of 3×105 ... Isolation of GFP+ cells from the HT-29 cell line. To confirm the above results, the GFP expression levels of selected cells ... Cell culture. Human colorectal cancer cells HT-29 were obtained from Shanghai Cell Collection, the Chinese Academy of Science. ...https://www.spandidos-publications.com/10.3892/ijo.2015.3138
Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon...
The secreted KiSS1 peptide inhibited human HT-29 cells' proliferation and migration probably by invoking, or mediating the cell ... However, whether this peptide can be functionally expressed in LAB and exert activity on cancer cells, thus providing a ... Our results suggest that L. lactis is an ideal cell factory for secretory expression of tumor metastasis-inhibiting peptide ... Furthermore, the expressed KiSS1 was shown to induce HT-29 cell morphological changes, apoptosis and reduce the expression of ...https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0506-7
Celecoxib decreases expression of the adhesion molecules ICAM-1 and VCAM-1 in a colon cancer cell line (HT29)
... reduced ICAM-1 and VCAM-1 expression in HT29 cells dose-dependently. Adhesion of HT29 cells to FCS-coated plastic wells was ... Celecoxib decreases expression of the adhesion molecules ICAM-1 and VCAM-1 in a colon cancer cell line (HT29). ... We analysed the effect of celecoxib on ICAM-1 and VCAM-1 protein and mRNA expression in HT29 cells. Experiments were performed ... We evaluated adhesion of HT29 cells to FCS-coated plastic wells in the presence of celecoxib or MAPK inhibitors. Furthermore, ...https://insights.ovid.com/bjpha/200803010/00002401-200803010-00005
Distinct classes of phosphatidylinositol 3'-kinases are involved in signaling pathways that control macroautophagy in HT-29...
In contrast, an increase in the class III PI3K product (phosphatidylinositol 3-phosphate), either by feeding cells with a ... protein signaling are required in the control macroautophagy in HT-29 cells. In conclusion, our results demonstrate that ... caused by either feeding cells with synthetic lipids (dipalmitoyl phosphatidylinositol 3, 4-bisphosphate and dipalmitoyl ... of this study was to identify PI3Ks involved in the control of macroautophagic sequestration in human colon cancer HT-29 cells ...https://www.semanticscholar.org/paper/Distinct-classes-of-phosphatidylinositol-3%27-kinases-Petiot-Ogier-Denis/4e4f7be1f2439af3732775c1949cb3dff31d0a0d
Sulforaphane, a Naturally Occurring Isothiocyanate, Induces Cell Cycle Arrest and Apoptosis in HT29 Human Colon Cancer Cells |...
Sulforaphane-treated HT29 Cells Are Blocked in G2-M Phase of the Cell Cycle and Express High Levels of Cyclin A and B1.. Cell ... 15 ). HT29 cells were purchased from European Collection of Cell Culture (Salisbury, United Kingdom). Stock cells were ... Here we show that in highly proliferative HT29 cells, sulforaphane induces a cell cycle arrest, followed by cell death. This ... In this study, we investigated the effect of sulforaphane on nonsynchronized HT29 cells. When HT29 cells were incubated for 48 ...http://cancerres.aacrjournals.org/content/60/5/1426
Kynurenine promotes the goblet cell differentiation of HT-29 colon carcinoma cells by modulating Wnt, Notch and AhR signals
When we cultured HT-29 cells in RPMI and DMEM, it was observed that most of the HT-29 cells differentiated into goblet cells ... Differentiation of goblet cells from HT-29 cells is induced in DMEM. HT-29 cells, which are routinely maintained in RPMI or MEM ... DMEM induces goblet cell differentiation from HT-29 colon cancer stem cells. (A) HT-29 cells seeded into a 24-well plate at a ... is expressed in HT-29 cells (22). Thus, we examined whether KN stimulates goblet cell differentiation. HT-29 cells cultured in ...https://www.spandidos-publications.com/10.3892/or.2018.6266
An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells
... Adamczak ... was studied using a novel in vitro method allowing the liposomes to interact with a mucus-producing confluent HT29-MTX cell- ... MTT viability and paracellular permeability tests were conducted on the same cell-line. The alginate-coated liposomes achieved ... but also displayed a higher probability of cell-irritation. The chitosan-coated liposomes displayed the highest potential for ...https://www.duo.uio.no/handle/10852/58497
Protective activity of butyrate on hydrogen peroxide-induced DNA damage in isolated human colonocytes and HT29 tumour cells :...
At 15 μM H2O2 DNA damage in HT29 and HT29 19A cells was significantly lower than that observed in normal colonocytes (P , 0.01 ... in HT29 and 30 and 80% in HT29 19A, respectively. Treatment of cells with a mixture of 25 mM acetate + 10.4 mM propionate + ... on DNA damage induced by H2O2 in isolated human colonocytes and in two human colon tumour cell lines (HT29 and HT29 19A). Human ... on DNA damage induced by H2O2 in isolated human colonocytes and in two human colon tumour cell lines (HT29 and HT29 19A). Human ...http://oxfordindex.oup.com/view/10.1093/carcin/22.10.1675
Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells. - PubMed - NCBI
Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells.. Richter JF1, ... on intestinal transport and barrier function in monolayers of HT-29/B6 human colon cells using the Ussing technique to ...https://www.ncbi.nlm.nih.gov/pubmed/19372105?dopt=Abstract
Eribulin Does Not Prevent Epithelial-to-Mesenchymal Transition in HT-29 Intestinal Epithelial Cells - Zurich Open Repository...
Eribulin Does Not Prevent Epithelial-to-Mesenchymal Transition in HT-29 Intestinal Epithelial Cells ... Eribulin Does Not Prevent Epithelial-to-Mesenchymal Transition in HT-29 Intestinal Epithelial Cells. Inflammatory Intestinal ... and Eribulin treatment does not decrease the mRNA expression of EMT markers in HT-29 monolayers nor prevent EMT in HT-29 ... and Eribulin treatment does not decrease the mRNA expression of EMT markers in HT-29 monolayers nor prevent EMT in HT-29 ...https://www.zora.uzh.ch/id/eprint/158473/
Chemopreventive effect of protein extract of Asterina pectinifera in HT-29 human colon adenocarcinoma cells | Springer for...
Chemopreventive effect of protein extract ofAsterina pectinifera in HT-29 human colon adenocarcinoma cells. ... QR and GST activity increased in HT-29 human colon adenocarcinoma cells increased that had been exposed to 4 concentrations of ... Liu, X. H., Wiley, H. S., and Meikle, A. W., Androgens regulate proliferation of human prostate cancer cells in culture by ... COX-2 expression was also inhibited in cells exposed to this extract. These results suggest that this protein extract ofA ...https://rd.springer.com/article/10.1007/BF02969395