In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP
... Xavier Gaume,1 Karine Monier,1 Françoise Argoul,1,2 ... Nucleolin possesses, in vitro, chromatin co-remodeler and histone chaperone activities which could explain numerous functions ... Thus, in live cells, nucleolin could play a role in chromatin accessibility by its histone chaperone and co-remodeling ... Changes in histone dynamics occurring in nucleolin silenced cells were measured by FRAP experiments on eGFP-tagged histones ( ...https://www.hindawi.com/journals/bri/2011/187624/abs/
The Yeast Histone Chaperone Chromatin Assembly Factor 1 Protects Against Double-Strand DNA-Damaging Agents | Genetics
The histone chaperone chromatin assembly factor 1 (CAF-1) mediates histone H3-H4 assembly during DNA replication and nucleotide ... This is mediated in part by the histone chaperone chromatin assembly factor 1 (CAF-1), which deposits histones H3 and H4 onto ... Adkins, M. W., S. R. Howar and J. K. Tyler, 2004 Chromatin disassembly mediated by the histone chaperone Asf1 is essential for ... Adkins, M. W., and J. K. Tyler, 2004 The histone chaperone Asf1p mediates global chromatin disassembly in vivo. J. Biol. Chem. ...http://www.genetics.org/content/171/4/1513
Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1...
Results We established a strain of S. cerevisiae lacking the histone chaperone ASF1/CIA1. This disruptant, asf1/cia1, arrested ... The factor hCIA interacts with hCCG1 and functions as a histone chaperone in mammalian cells; its homologue in yeast is Asf1p/ ... Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1/ ... elegans homologs of the histone chaperone Asf1 in germline DNA replication, Developmental Biology, 2009, 329, 1, 64. CrossRef ...http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2443.2001.00487.x/abstract?globalMessage=0
Nucleosome Assembly Proteins Get SET to Defeat the Guardian of Chromosome Cohesion
2009) Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during ... Das C, Tyler JK, Churchill ME (2010) The histone shuffle: histone chaperones in an energetic dance. Trends Biochem Sci 35: 476- ... SET and Nap1 have both been extensively studied as histone chaperones; they can also associate with histone acetyltransferases ... or could their histone chaperone activity, or binding to histone-modifying enzymes, play a role? The future use of separation ...http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1003829
The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions |...
2005). The histone H3.3 chaperone HIRA is essential for chromatin assembly in the male pronucleus. Nature 437, 1386-1390. ... 2010). The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of ... One histone variant, H3.3, has been studied extensively. Although histone H3.3 differs by only four amino acids from canonical ... Histone H3.1 is assembled into chromatin during DNA replication, whereas deposition of histone H3.3 occurs throughout the cell ...http://dev.biologists.org/content/139/24/4582
British Library EThOS: Saccharomyces cerevisiae proteins Rtt109p and Esc2p : two novel regulators of genome stability
... and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. ... Furthermore, I show that, as for Asf1p, Rtt109p is required for histone H3 acetylation on lysine 56 (K56) in vivo. Moreover I ... These data establish Rtt109p as a member of a new class of histone acetyltransferases and show that its actions are critical ...http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598656
Connecting the histone acetyltransferase complex SAS-I to the centromere in S. cerevisiae
... two histone chaperones that assemble histones H3 and H4 into nucleosomes. Our findings further suggest a role of Cac1 ... The essential histone H3 variant Cse4 plays a crucial role at the centromere in S. cerevisiae, where it replaces histone H3 in ... We found in our study that the histone H3 variant was able to interact over its unique N-Terminus with two subunits of the ... An important question resulted from our studies: is Sas2 able to acetylate the histone H3 variant Cse4 ? We have circumstantial ...https://edoc.hu-berlin.de/handle/18452/15787
ASF1 like histone chaperone - Wikipedia
... the ASF1 like histone chaperone family of proteins includes the yeast and human ASF1 proteins. These proteins have histone ... "A human homologue of yeast anti-silencing factor has histone chaperone activity". Genes Cells. 5 (3): 221-33. doi:10.1046/j. ... "Structure and function of the conserved core of histone deposition protein Asf1". Curr. Biol. 13 (24): 2148-58. doi:10.1016/j. ... chaperone activity. ASF1 participates in both the replication-dependent and replication-independent pathways. The three- ...https://en.wikipedia.org/wiki/ASF1_like_histone_chaperone
Mayo Researchers Provide Atomic View of a Histone Chaperone - Healthcanal.com : Healthcanal.com
... scientists knew that the histone chaperone Rtt106 helped in the deposition of histones - specifically, a complex of histones H3 ... Associated with the histones is another group of proteins called histone chaperones, which promote the proper assembly or ... This is the first time anyone has described this mode of specific association between a histone chaperone and a modified ... Home Medical Breakthroughs Mayo Researchers Provide Atomic View of a Histone Chaperone ...https://www.healthcanal.com/medical-breakthroughs/27185-mayo-researchers-provide-atomic-view-of-a-histone-chaperone.html
Enhanced expression of histone chaperone APLF associate with breast cancer | Springer for Research & Development
DNA damage-specific histone chaperone Aprataxin PNK-like factor (APLF) regulates mesenchymal-to-epithelial transition (MET) ... As a histone chaperone, APLF specifically bind to histones H3/H4 tetramer and could recruit variants of histone H2A at the ... Histone chaperones in nature could interact with histone modifying enzymes and thereby could modulate different histone ... DNA repair factor APLF is a histone chaperone. Mol Cell. 2011;41:46-55.CrossRefPubMedPubMedCentralGoogle Scholar ...https://rd.springer.com/article/10.1186/s12943-018-0826-9
Functional Analysis of Hif1 Histone Chaperone in Saccharomyces cerevisiae | G3: Genes | Genomes | Genetics
2015 Dissecting the Molecular Roles of Histone Chaperones in Histone Acetylation by Type B Histone Acetyltransferases (HAT-B). ... 2012 Histone chaperones link histone nuclear import and chromatin assembly. Biochim. Biophys. Acta 1819: 277-289. doi:10.1016/j ... 2007 Histone chaperones: an escort network regulating histone traffic. Nat. Struct. Mol. Biol. 14: 997-1007. doi:10.1038/ ... 2016 The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats ...http://www.g3journal.org/content/8/6/1993
Cardiomyocyte-specific conditional knockout of the histone chaperone HIRA in mice results in hypertrophy, sarcolemmal damage...
2014). Histone chaperones: assisting histone traffic and nucleosome dynamics. Annu. Rev. Biochem. 83, 487-517. doi:10.1146/ ... HIRA is the histone chaperone responsible for replication-independent incorporation of histone variant H3.3 within gene bodies ... 2013). Histone chaperones in nucleosome assembly and human disease. Nat. Struct. Mol. Biol. 20, 14-22. doi:10.1038/nsmb.2461. ... 2015). Histone chaperone HIRA in regulation of transcription factor RUNX1. J. Biol. Chem. 290, 13053-13063. doi:10.1074/jbc. ...http://dmm.biologists.org/content/9/3/335
Histone chaperones in Arabidopsis and rice: genome-wide identification, phylogeny, architecture and transcriptional regulation ...
For the largest histone chaperone family, NAP, phylogenetic reconstruction suggested the presence of two distinct groups in ... Phylogenetic analyses of histone chaperones from diverse organisms including representative species from each of the major ... In contrast to their animal and yeast counterparts, not much is known about plant histone chaperones. To gain insights into ... Taken together, our findings shed light onto the possible evolutionary trajectory of plant histone chaperones and present novel ...https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0414-8
Epigenetic modulation of interferon-stimulated gene expression by the histone chaperone HIRA and the variant histone H3.3 | NIH...
Epigenetic modulation of interferon-stimulated gene expression by the histone chaperone HIRA and the variant histone H3.3. ... Histone variant H3.3 is implicated in diverse biological processes that involve transcriptional regulation and maintenance of ... Our results highlight role of H3.3-HIRA complex in inducible transcription and show that the histone H3.3 occupancy serves as a ... Further, these changes coincided with changes in histone modification marks, like H3K4me3, H3K4me1, H3K27Ac, H3K36me3 and that ...https://researchfestival.nih.gov/2016/posters/epigenetic-modulation-interferon-stimulated-gene-expression-histone
Histone chaperone networks shaping chromatin function - Danish National Research Database
We emphasize how chaperones cooperate in the histone chaperone network and via co-chaperone complexes to match histone supply ... In this way, the chaperoning of soluble histones is a key determinant of histone availability and fate, which affects all ... The association of histones with specific chaperone complexes is important for their folding, oligomerization, post- ... Here, we review the distinct structural and functional properties of the expanding network of histone chaperones. ...http://www.forskningsdatabasen.dk/en/catalog/2350695468
E-GEOD-40717 - Genome-wide targets of conserved histone chaperone Asf1 in budding yeast - OmicsDI
... through its histone chaperone activity, helps chromatin closing/opening during DNA replication, repair, recombination and ... Asf1, through its histone chaperone activity, helps chromatin closing/opening during DNA replication, repair, recombination and ... Genome-wide targets of conserved histone chaperone Asf1 in budding yeast. Ontology highlight ... Project description:ChIP-on chip assays to measure the change in histone acetylation over the yeast genome, in ASF1, SET2 and ...https://www.omicsdi.org/dataset/arrayexpress-repository/E-GEOD-40717
Shigeaki Kato (the University of Tokyo): A histone chaperone, DEK, transcriptionally coactivates a nuclearreceptor
A histone chaperone, DEK, transcriptionally coactivates a nuclearreceptor.. Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, ...http://katolab-imagefraud.blogspot.co.uk/2012/01/pka-dependent-regulation-of-histone_15.html
Developmental roles of histone H3 variants and their chaperones. - PubMed - NCBI
Developmental roles of histone H3 variants and their chaperones.. Filipescu D1, Szenker E, Almouzni G. ... promoted by dedicated histone chaperones. Moreover, their individual modifications and binding partners provide distinct ... Histone H3 variants contribute to chromatin dynamics through the timing and sites of their incorporation, ... Future challenges will be to gain a comprehensive picture of H3 variants and their chaperones during development and ...https://www.ncbi.nlm.nih.gov/pubmed/23830582
Characterization of the Pneumocystis carinii histone acetyltransferase chaperone proteins PcAsf1 and PcVps75. - Semantic Scholar
Efficient Rtt109 acetyltransferase activity also requires a histone chaperone, vacuolar protein sorting 75 (Vps75), as well as ... Together, our results indicate that the functions of the Rtt109-Asf1-Vps75 complex in the acetylation of histone H3 lysine 56 ... Lastly, we observed that purified PcAsf1 and PcVps75 proteins enhance the ability of PcRtt109 to acetylate histone H3-H4 ... We demonstrate that both P. carinii Asf1 (PcAsf1) and PcVps75 can bind histones. Furthermore, when Pcasf1 is expressed ...https://www.semanticscholar.org/paper/Characterization-of-the-Pneumocystis-carinii-histo-Pupaibool-Kottom/dc25858aeafdf6afcc2dfff7753e35742f4583db
The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats
... ... Here we investigate the interaction between the histone chaperone sNASP and its histone H3 substrate. We find that sNASP binds ... The histone chaperone sNASP binds a conserved peptide motif within the globular core of histone H3 through its TPR repeats. In ... Key to this process is a group of proteins termed histone chaperones that guide the thermodynamic assembly of nucleosomes by ...http://www.repo.uni-hannover.de/handle/123456789/695
"Molecular functions of the histone acetyltransferase chaperone complex" by Christopher E. Berndsen, Toshiaki Tsubota et al.
... functions with the histone chaperone Asf1 to acetylate lysine K56 on histone H3 (H3K56), a modification associated with newly ... Together, these data provide evidence for a multifunctional HAT-chaperone complex that acetylates histone H3 and deposits H3-H4 ... In vitro analysis of Rtt109 revealed that Vps75, a Nap1 family histone chaperone, could also stimulate Rtt109-dependent ... We find that Vps75 stimulates the kcat of histone acetylation by approximately 100-fold relative to Rtt109 alone and enhances ...https://escholarship.umassmed.edu/pgfe_pp/88/
Histone H2A.Z-specific chaperone elisa and antibody
Recombinant Protein and Histone H2A.Z-specific chaperone Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and ... Shop Histone H2A.Z-specific chaperone ELISA Kit, ... Histone H2A.Z-specific chaperone chz-1. Histone H2A.Z-specific ... Histone H2A.Z-specific chaperone chz1. Histone H2A.Z-specific chaperone chz1 ELISA Kit. Histone H2A.Z-specific chaperone chz1 ... Histone H2A.Z-specific chaperone chz-1 Recombinant. Histone H2A.Z-specific chaperone chz-1 Antibody. Forms a chaperone-bound ...https://www.mybiosource.com/protein_family.php?root=histone-h2a-z-specific-chaperone
The Dinshaw Patel Lab | Sloan Kettering Institute
Histone Chaperones The biology of histone proteins encompasses their synthesis in the cytosol, nuclear import and incorporation ... Histone chaperones represent a structurally and functionally diverse family of histone-binding proteins that prevent ... Current efforts are aimed at addressing the specificity underlying histone chaperone binding to histones H3-H4, H2a-H2b and ... DNA Methylation and Cross-talk with Histone Methylation Methylation of cytosine in the CpG context has pronounced effects on ...https://www.mskcc.org/research/ski/labs/dinshaw-patel/projects
Chaperone co-inducer BGP-15 inhibits histone deacetylases and enhances the heat shock response through increased chromatin...
Chaperone co-inducer BGP-15 inhibits histone deacetylases and enhances the heat shock response through increased chromatin ... HATs histone acetyltransferases, HDACs histone deacetylases, HDACi HDAC inhibitors, HSPs heat shock proteins ... Cell Stress Chaperones 17:517-521. doi: 10.1007/s12192-012-0327-5 CrossRefPubMedPubMedCentralGoogle Scholar ... Cell Stress Chaperones 20:185-201. doi: 10.1007/s12192-014-0536-1 CrossRefPubMedPubMedCentralGoogle Scholar ...https://link.springer.com/article/10.1007/s12192-017-0798-5
ASF1A Gene - GeneCards | ASF1A Protein | ASF1A Antibody
Anti-Silencing Function 1A Histone Chaperone, including: function, proteins, disorders, pathways, orthologs, and expression. ... Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and ... Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and ... Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=ASF1A