Research Labs and Institutes | Johns Hopkins Medicine
... to develop high-throughput and screening assays for identifying novel therapeutic compounds; to use proteomics and lipidomics ...
mAb Solutions for Biopharma Research - Eppendorf Canada
For high-throughput screening assays. *Screen up to 81 flasks in parallel ... Transfer liquids, bacteria and cells with reliable pipettes at highest accuracy and precision. Explore pipetting solutions ... From producing target antigens up to cell line development, you benefit from optimal centrifugation, smart lab solutions, high- ...
Automated Laboratory Robotics
| Thermo Fisher Scientific
Engineer III CMC job with Takeda | 2808370
Materials | Free Full-Text | Red and Near-Infrared Absorbing Dicyanomethylene Squaraine Cyanine Dyes: Photophysicochemical...
Comparison of alamar blue and MTT assays for high through-put screening. Toxicol. Vitr. 2004, 18, 703-710. [Google Scholar] [ ... The higher concentration of dimethyl sulfoxide, used in cell viability assays, did not produce statistically significant ... All dyes displayed high absorption within the so-called "phototherapeutic window". The dyes under study showed singlet oxygen ... Although the symmetrical dye 1 in our fluorescence study presented a high affinity for human and bovine serum albumin and in ...
JCI - CD73 immune checkpoint defines regulatory NK cells within the tumor microenvironment
Development of a novel ectonucleotidase assay suitable for high-throughput screening. J Biomol Screen. 2012;17(7):993-998. ... per million cells and 50 IU/mL IL-2 for 48 hours before suppression assay. For the suppression assay, CD73+ and CD73- NK cells ... G) IFN-γ+CD4+ T cells in a suppression assay comparing sorted CD73+ NK cells versus CD73- NK cells with or without GPB730 ... CD73-) calculated based on percentage of CFSEhiIFN-γ- responder CD4+ T cells in autologous coculture. A sample size of n = 5 ...
Cellular Analysis via High Content Screens
Cell-based assays are becoming increasingly popular for small molecule discovery in high-throughput screening. Advanced high- ... As cell-based assays are used more commonly in robotic high-throughput compound screening, cells themselves have become ... High-throughput screening has been around for a while, but it has only been in the last few years that doing so on a cellular ... Screening using cell-based assays has been performed for many years, but recent technologies allow screening of cells that are ...
Publications at this Location : USDA ARS
High-throughput micro plate assays for screening flavonoid content and DPPH-scavenging activity in sorghum bran and flour (Peer ... Effect of high molecular weight glutenin subunit allelic composition on wheat flour tortilla quality (Peer Reviewed Journal) ( ... Estimating the relative effects of the endosperm traits of waxy and high protein digestibility on yield in grain sorghum (Peer ...
Publications at this Location : USDA ARS
High-throughput micro plate assays for screening flavonoid content and DPPH-scavenging activity in sorghum bran and flour (Peer ... Effect of high molecular weight glutenin subunit allelic composition on wheat flour tortilla quality (Peer Reviewed Journal) ( ... Estimating the relative effects of the endosperm traits of waxy and high protein digestibility on yield in grain sorghum (Peer ... Analysis of Glu-1 deletion lines reveals the importance of high molecular weight glutenin subunits 7+9 Glu-B1 in wheat flour ...
Cell Culture Screening Solution - Eppendorf
Discover an incubator solution for high throughput screening assays. ... Cell Culture Screening Solution. Recommended incubator shaker configuration for high-throughput screening assays. ... Screening assays involve the exposure of PBMCs to new potential antibodies, e.g. to test for toxicity with cell-based assays or ... We dedicated the Cell Culture Screening Solution exactly for these workflow steps - as it supports reproducible assay ...
RPMI-1640 Medium liquid with L-glutamine Sigma
To identify such compounds, we developed a high-throughput screening assay. View All Related Papers ... Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and ... Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium ...
Cardiovascular Health Effects Innovation
High-throughput bioactivity assessment via CardioToxPi. Map Tox21 high-throughput screening (HTS) assays to CV failure modes ... High-throughput screening to identify chemical cardiotoxic potential. Chem Res Toxicol 34(2):566-583.. ... Existing knowledge and medium- to high- throughput bioactivity screening will be leveraged to provide insights into CV-relevant ... The assay systems used are aligned to known human CV failure modes and reflect human biology as much as the complexity of the ...
Handbook of Drug Screening, 2nd edition edited by Ramakrishna Seethala and Litao Zhang
Handbook of Drug Screening, 2nd edition: Books: edited by Ramakrishna Seethala and Litao Zhang ... Emerging Novel High-Throughput Screening Technologies for Cell-Based Assays *In Vitro Strategies for Ion Channel Screening in ... Key Factors for Successful High-Throughput Screening *Critical Components in High-Throughput Screening: Challenges and New ... Practical Approach to Quantitative High-Throughput Screening *Enabling the Large-Scale Analysis of Quantitative High-Throughput ...
Innovative Technologies for Tomorrow's Discoveries
Assay Guidance Workshop for High-Throughput Screening and Lead Discovery. *3D Culture Models and Organoids for Drug Discovery ...
Advance Search
| Bentham Science
A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity | Protocol
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos… * Dissection and Downstream Analysis of ... High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function… ... High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function… ... Journal / Biology / A Method of Permeabilization of Drosophila Embryos for Assays of Small Molecule Activity… ...
Frontiers | Highly Parallelized, Multicolor Optogenetic Recordings of Cellular Activity for Therapeutic Discovery Applications...
... the Swarm is a novel high-throughput all-optical system capable of collecting information-dense data from optogenetic assays in ... To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for ... and throughput to enable their application to broader drug screening applications. To address this challenge, we developed the ... and throughput to enable their application to broader drug screening applications. To address this challenge, we developed the ...
Molecular Devices introduces fatty acid uptake assay to target metabolic diseases
... homogenous in vitro assay for fatty acid uptake for use in high- throughput screening and metabolic disease research. ... the first assay kit that addresses the need for a single-step, ... announced the launch of its QBT Fatty Acid Uptake Assay Kit, ... High Throughput, High-throughput screening, in vitro, Insulin, Laboratory, Metabolic Disease, Metabolism, Obesity, Research, T- ... We believe that the QBT Fatty Acid Uptake Assay Kit will enable high-throughput screening of these drug targets and enhance ...
Chemical Biology Core Facility - Small molecules play essential roles in many areas of basic research and are often used to...
Assay development and high-throughput screening. *Binding affinity studies for small molecules and antibodies using the Biacore ... High Throughput Screening (HTS) allows a researcher to quickly conduct thousands of tests against a biological target through ... Computational screening using ligand-based and structure-based design strategies. *Medicinal chemistry optimisation of hits to ... Computational screening using ligand-based and structure-based design strategies. *Managing compound acquisition through our ...
STEMdiff™ Midbrain Organoid Differentiation Kit | STEMCELL Technologies
Achieve long-term culture survival for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays ... for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays. ... Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid ... serum-free cell culture media can prevent organoid fusion and support the generation of over 500 organoids per kit for higher- ...
University of Arkansas for Medical Sciences, Department of Biochemistry
Experience with drug screening and high throughput assays is a valuable asset. ... As a Post-Doctoral Fellow in our laboratory, you will have the opportunity to work on high-impact projects, collaborate with ... Proficiency in cell culture, transfection, DNA repair assays, cell imaging, western blotting, NGS, and other relevant ... Conducting molecular and cell biology assays in both human and mouse cell lines. ...
KAKEN - Research Projects | Humanized model mouse based on the structures of proteins (KAKENHI-PROJECT-17591081)
Journal Article] Development of Fluorescence-linked Immunosorbent Assay for High Throughput Screening of Interferon-γ2006. *. ... Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma2006. ... Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma.2006 ... Journal Article] Development of fluorescence-linked immunosorbent assay for high throughput screening of interferon-gamma.2006 ...
Complex Technologies and Therapeutics Development for Mental Health Research and Practice (R41/R42 - Clinical Trial Optional) |...
development of novel ligand screening assays (such as computational, high throughput, genetic, molecular/cellular, or whole ... High Throughput Tools for Neuroscience and Mental Health Research: Many of the common technologies used in neuroscience and ... a unique opportunity is available to bring these technology and biomedical areas together to develop innovative high throughput ... considered appropriate to this topic would include those proposing research and development of tools for high throughput ...
Interventions to Reduce Risk for Pathogen Spillover and Early Disease Spread to Prevent Outbreaks, Epidemics, and Pandemics -...
... surveillance should include pathogen-specific molecular and serologic diagnostic assays and unbiased high-throughput screening ... Faust CL, McCallum HI, Bloomfield LSP, Gottdenker NL, Gillespie TR, Torney CJ, et al. Pathogen spillover during land conversion ... Programs funded by high-income countries and focused on bilateral aid remain critical, but no country should carry the costs ... In China, the price for wildlife is generally 2- to 5-fold higher than for pork, the most common animal protein source in the ...
Berloque Dermatitis: Background, Pathophysiology, Etiology of Berloque Dermatitis
High throughput screening (HTS) for phototoxicity hazard using the in vitro 3T3 neutral red uptake assay. Toxicol In Vitro. ... Baran R, Maibach HI. Textbook of Cosmetic Dermatology. 5th ed. Boca Raton, Fla: CRC Press; 2017. ... EEC COLIPA project on in-vitro phototoxicity testing - first results obtained with BALB/C 3T3 cell phototoxicity assay. Toxicol ... Marzulli FN, Maibach HI. Perfume phototoxicity. J Soc Cosmetic Chem. 1970. 21:695-715. ...
NCATS Call for Proposals for Collaboration for the NIH HEAL Initiative | National Center for Advancing Translational Sciences
... quantitative high-throughput and high-content screening; robotic automated cell culture; multiscale assay development; 3-D ... assay development, biomolecular screening, automated biology, medicinal chemistry, cheminformatics, data science and ... physiologically relevant in vitro screening and characterization systems; high-efficiency synthesis and testing of small ... The NCATS-led NIH HEAL Initiative Human Cell-Based Screening Platforms and Novel Drugs to Treat Pain, Addiction and Overdose ( ...
eXpress Kits
Use eXpress assay kits to screen your therapeutic candidates and obtain either functional or binding data in-house quickly and ... Brochures & Flyers High-Throughput Screening & Lead Optimization Products Robust Cell-Based Assays to Identify Therapeutic Hits ... Functional and Binding Analysis - Perform quick, high-throughput cell-based assay binding and mechanism-of-action (MOA) ... Target Engagement Assays Cell-based assays to confirm compound cell entry, protein target binding, screen & rank inhibitors, & ...
Assay-ready Patient-derived organoids (PDOs) and High-throughput 3D Imaging to Drug Discovery
... and high-throughput 3D imaging to advance drug discovery for 3D tissues in the human body. ... This case study is for assay-ready patient-derived organoids (PDOs) ... The use of assay-ready organoids, together with automation of high-throughput screening and quantification assays will ... A case study for assay-ready patient-derived organoids (PDOs) and high-throughput 3D imaging to advance drug discovery. ...