Introduction: cell-based assays for high-throughput screening. | Broad InstituteIntroduction: cell-based assays for high-throughput screening. | Broad Institute

Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the ... Animals, Biological Assay, Cells, Combinatorial Chemistry Techniques, Drug Evaluation, Preclinical, Humans, Small Molecule ... Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay ... history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in ...
more infohttps://www.broadinstitute.org/publications/broad3403

Patent US6558944 - High throughput screening assay systems in microscale fluidic devices - Google PatentsPatent US6558944 - High throughput screening assay systems in microscale fluidic devices - Google Patents

In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their ... The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening ... High throughput screening assay systems in microscale fluidic devices. US5948227. Dec 17, 1997. Sep 7, 1999. Caliper ... High-throughput screening assay systems in microscale fluidic devices. WO1998000705A1. Jun 25, 1997. Jan 8, 1998. Caliper ...
more infohttp://www.google.com/patents/US6558944?dq=5,742,768

EPA ToxCast - Describing High-Throughput Screening Assays Idea Generation ContestEPA ToxCast - Describing High-Throughput Screening Assays Idea Generation Contest

Please remember that we are looking for a listing of relevant high-throughput screening assays, scientific studies and listing ... EPA ToxCast - Describing High-Throughput Screening Assays Idea Generation Contest. ConceptualizationTCO14 ... Please submit a document describing all found high-throughput screening assays, scientific studies and/or listing of the ... from online sources for determining relevant high-throughput screening assays and to figure out the existing LELs predicted by ...
more infohttps://www.topcoder.com/challenges/30036103/

Development and Optimization of a High-Throughput Screening Assay for Rapid Evaluation of Lipstatin Production by Streptomyces...Development and Optimization of a High-Throughput Screening Assay for Rapid Evaluation of Lipstatin Production by Streptomyces...

Development and Optimization of a High-Throughput Screening Assay for Rapid Evaluation of Lipstatin Production by Streptomyces ... Gong G, Sun X, Liu X et al (2007) Mutation and a high-throughput screening method for improving the production of Epothilones ... Zeng W, Du G, Chen J et al (2015) A high-throughput screening procedure for enhancing α-ketoglutaric acid production in ... Mateos-Díaz E, Rodríguez JA, de los Ángeles Camacho-Ruiz M, Mateos-Díaz JC (2012) High-throughput screening method for Lipases/ ...
more infohttps://link.springer.com/article/10.1007/s00284-017-1420-x

Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitorsAuxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors

... we have set up a High Throughput Screening assay for the identification of stringent response inhibitors. By screening 17,500 ... Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors. ... thus motivating future application of our screening assay to larger and more diverse molecular libraries. ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A1052657&c=60&searchType=SIMPLE&language=sv&query=&af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22801%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells. | Sigma-AldrichTwo High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells. | Sigma-Aldrich

Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells.. [Kristin P Leister, Ruili Huang, Bonnie L Goodwin, ... A simple and quick measurement of TNF-α production in a cell based assay is needed for high throughput screening to identify ... The results demonstrated that both homogeneous TNF-α assays are robust and suitable for high throughput screening. ... and AlphaLISA assay formats. We have validated the HTRF based TNF-α assay in a 1536-well plate format by screening a library of ...
more infohttps://www.sigmaaldrich.com/catalog/papers/21643507

Exploring decaprenyl-phosphoryl ribose epimerase (DprE1) as a validated target for TB drug discovery: Assay development, high...Exploring decaprenyl-phosphoryl ribose epimerase (DprE1) as a validated target for TB drug discovery: Assay development, high...

The novel assay will be used for high-throughput screening of libraries of commercially available compounds in order to find ... high-throughput screening and search for novel DprE1 inhibitor scaffolds. From 2010-05-01 to 2012-04-30, closed project ... high-throughput screening and search for novel DprE1 inhibitor scaffolds ... in order to overcome the complexity and low-throughput of the currently used TLC assay. ...
more infohttps://cordis.europa.eu/project/rcn/96831_en.html

Dr. Kevin Pfleger Discusses Novel nanoBRET Assay Development for High Throughput Screening | SelectScienceDr. Kevin Pfleger Discusses Novel nanoBRET Assay Development for High Throughput Screening | SelectScience

... novel nanoBRET assay that was developed in collaboration with BMG Labtech and Promega. ... Kevin Pfleger Discusses Novel nanoBRET Assay Development for High Throughput Screening. 6 November 2017 ... High-Throughput Screening of Affimer Proteins for Use as Affinity Ligands... Watch Now ... Latest: AnywhereRaman Plug and Play Unit Combines Ease with High Quality Raman Measurements ...
more infohttp://www.selectscience.net/SelectScience-TV/Videos/dr-kevin-pfleger-discusses-novel-nanobret-assay-development-for-high-throughput?videoID=3231

Validation of miniaturized one-step reverse transcription qPCR assays for high-throughput screening and comparison to a...Validation of miniaturized one-step reverse transcription qPCR assays for high-throughput screening and comparison to a...

Assay Drug Dev Technol. 2015 Mar;13(2):94-101. doi: 10.1089/adt.2014.630. Comparative Study; Evaluation Studies; Validation ... Validation of miniaturized one-step reverse transcription qPCR assays for high-throughput screening and comparison to a ... We also demonstrate a miniaturized and high-throughput workflow that could enable future application of this sensitive assay ... but not in the context of high-throughput screening owing to limitations on workflow, cost of reagents, and miniaturization ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/25785772

Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with...Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with...

Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with ... Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with ... Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with ... Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/29425233

PLOS ONE: A FRET-Based High Throughput Screening Assay to Identify Inhibitors of Anthrax Protective Antigen Binding to...PLOS ONE: A FRET-Based High Throughput Screening Assay to Identify Inhibitors of Anthrax Protective Antigen Binding to...

This work represents the first reported high throughput screening assay targeting CMG2 to identify possible inhibitors of both ... high throughput screening assay designed to identify molecules that inhibit binding of PA to CMG2. Molecules identified in the ... The assay to screen for inhibitors of this protein-protein interaction is sensitive and robust, with observed Z values as high ... Preliminary screens conducted with a library of known bioactive compounds identified tannic acid and cisplatin as inhibitors of ...
more infohttp://journals.plos.org/plosone/article/comments?id=10.1371/journal.pone.0039911

A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of...A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of...

A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of ... Herein, we describe the development of a high throughput screening assay for the identification of molecules that are ... Thus, the AK assay is more sensitive than traditional growth assays. We have validated the AK assay in the HTS setting using ... The screen of the FDA-approved library demonstrated that the AK assay is able to identify the vast majority of drugs with known ...
more infohttps://www.sigmaaldrich.com/catalog/papers/26098625

Development of a fluorescent intensity assay amenable for high-throughput screening for determining 15-lipoxygenase activity.Development of a fluorescent intensity assay amenable for high-throughput screening for determining 15-lipoxygenase activity.

... ... and thus amenable for high-throughput screening. The utility of DPPP as a marker for 15-lipoxygenase activity was demonstrated ... in enzyme or cellular assays using either a 96-well or a 384-well format. The assays described take advantage of the ability of ... Journal of Biomolecular Screening On the subject. Biomedical Laboratory Science/Technology Search outside of DiVA. GoogleGoogle ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:1186517

AID 504556 - High Throughput Screening Assay used to Identify Novel Compounds that Inhibit Mycobacterium Tuberculosis in 7H9...AID 504556 - High Throughput Screening Assay used to Identify Novel Compounds that Inhibit Mycobacterium Tuberculosis in 7H9...

High Throughput Screening Assay used to Identify Novel Compounds that Inhibit Mycobacterium Tuberculosis in 7H9 Media using ... BioAssay record AID 504556 submitted by Southern Research Specialized Biocontainment Screening Center: ...
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/504556

AID 624416 - TRFRET-based biochemical primary high throughput screening assay to identify small molecules that bind to the HIV...AID 624416 - TRFRET-based biochemical primary high throughput screening assay to identify small molecules that bind to the HIV...

TRFRET-based biochemical primary high throughput screening assay to identify small molecules that bind to the HIV-1-gp120 ... BioAssay record AID 624416 submitted by The Scripps Research Institute Molecular Screening Center: ...
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/624416

Repurposing Drugs for ADPKD: Assay Development and High-throughput Screening | NIH Research FestivalRepurposing Drugs for ADPKD: Assay Development and High-throughput Screening | NIH Research Festival

Repurposing Drugs for ADPKD: Assay Development and High-throughput Screening. Wednesday, September 13, 2017 - Poster Session I ... repurposing for ADPKD through the development of biologically relevant in vitro assays amenable for high-throughput screening ...
more infohttps://researchfestival.nih.gov/2017/posters/repurposing-drugs-adpkd-assay-development-and-high-throughput-screening

New High Throughput Screening (HTS) Assay to Aid Researchers in Their Search for Breakthrough Drugs Targeting Epigenetic...New High Throughput Screening (HTS) Assay to Aid Researchers in Their Search for Breakthrough Drugs Targeting Epigenetic...

... assay to aid researchers in their search for breakthrough drugs targeting epigenetic pathways. ... The AptaFluor™ SAH Methyltransferase Assay is an enabling new high throughput screening (HTS) ... The AptaFluor™ SAH Methyltransferase Assay is an enabling new high throughput screening (HTS) assay to aid researchers in their ... New High Throughput Screening (HTS) Assay to Aid Researchers in Their Search for Breakthrough Drugs Targeting Epigenetic ...
more infohttp://www.selectscience.net/product-news/new-high-throughput-screening-

Optimizing Performance of the Membrane-free, Oris™ Cell Migration Assay for High Throughput Screening using the BioTek Synergy™...Optimizing Performance of the Membrane-free, Oris™ Cell Migration Assay for High Throughput Screening using the BioTek Synergy™...

Cell Migration Assay for High Throughput Screening using the BioTek Synergy™ HT Multi-Mode Microplate Reader ... Optimizing Performance of the Membrane-free, Oris™ Cell Migration Assay for High Throughput Screening using the BioTek Synergy ... The Oris Cell Migration Assay based on a 96-well microplate design allows for sample throughput and assay performance suitable ... Cell Migration Assay is a microplate-based tool suitable for screening. The assay comprises a 96-well plate with silicone ...
more infohttps://www.biotek.com/resources/application-notes/optimizing-performance-of-the-membrane-free-oris-cell-migration-assay-for-high-throughput-screening-using-the-biotek-synergy-ht-multi-mode-microplate-reader/

Comparison of Corepressor Peptides in the Development of a High Throughput Screening Assay for Small Molecule Agonists of the...Comparison of Corepressor Peptides in the Development of a High Throughput Screening Assay for Small Molecule Agonists of the...

Conclusions: : We developed an assay in a format compatible with high-throughput screening of large chemical libraries. The ... Comparison of Corepressor Peptides in the Development of a High Throughput Screening Assay for Small Molecule Agonists of the ... Comparison of Corepressor Peptides in the Development of a High Throughput Screening Assay for Small Molecule Agonists of the ... S. Zimov, C. Mandavia, K. Petrukhin; Comparison of Corepressor Peptides in the Development of a High Throughput Screening Assay ...
more infohttps://iovs.arvojournals.org/article.aspx?articleid=2376824

Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer...Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer...

Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer ... Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer ... Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer ... Correction: An Image-Based, High-Throughput Screening Assay for Molecules that Induce Excess DNA Replication in Human Cancer ...
more infohttp://mcr.aacrjournals.org/content/early/2011/06/17/1541-7786.MCR-11-0249

Assay development in high throughput screening - 2 ECTS - 
			
	
			
			
				Chemical Biological Centre - 
			
			Umeå...Assay development in high throughput screening - 2 ECTS - Chemical Biological Centre - Umeå...

Course organised by LCBU and gives a practical basis for assay development in high throughput screening and is given as a ... "Introduction to high throughput screening". High throughput screening (HTS) is a technique used to identify biologically active ... Assay development in high throughput screening - 2 ECTS. Course organised by LCBU and gives a practical basis for assay ... The course gives a practical basis for assay development in high throughput screening and is given as a practical complement to ...
more infohttp://www.kbc.umu.se/english/courses/assay-development-in-high-throughput-screening---2-ects/

PA-07-320: Development of Assays for High-Throughput Drug Screening (R01)PA-07-320: Development of Assays for High-Throughput Drug Screening (R01)

Development of Assays for High-Throughput Drug Screening (R01) PA-07-320. NIDDK ... Title: Development of Assays for High-Throughput Drug Screening (R01). Announcement Type This is a reissue of PA-07-054, which ... However, experiments proposed in response to this FOA need not include the use of assays in high-throughput screens or even the ... NIMH: The NIMH is especially interested in applications to develop high-throughput screen (HTS) assays for novel clinically ...
more infohttps://grants.nih.gov/grants/guide/pa-files/PA-07-320.html

Download High Throughput Screening Assays In Toxicology 2016Download High Throughput Screening Assays In Toxicology 2016

... the WB intervened a intact download High Throughput Screening Assays in Toxicology in the developer, copy, audience, and CD of ... some chemicals process download High Throughput Screening Assays in epidemiological. Your download High Throughput Screening ... This download High Throughput Screening Assays in has a literary item to s posting average( MRI), and continues created to a ... A download High Throughput Screening Assays in is through the & from the bad look( Pinnacles Entrance), cited with area Books ...
more infohttp://lustige-weihnachtsgeschenke.de/wp-includes/ebooks/download-High-Throughput-Screening-Assays-in-Toxicology-2016.php

Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone...Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone...

Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone ... binding assay. Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis ... the sensitivity of the newly developed assay was slightly lower than the radio-ligand binding assay, however, throughput was ... For the first time a miniaturized fluorescence T4-TTR binding assay was developed in a 96 well microplate and tested with eight ...
more infohttps://brage.bibsys.no/xmlui/handle/11250/2579044

A Universal, Fully Automated High Throughput Screening Assay for Pyrophosphate and Phosphate Release from Enzymatic Reactions |...A Universal, Fully Automated High Throughput Screening Assay for Pyrophosphate and Phosphate Release from Enzymatic Reactions |...

Combinatorial Chemistry & High Throughput Screening. Title: A Universal, Fully Automated High Throughput Screening Assay for ... high throughput screening, pyrophosphatase, assay techniques, phosphate. Abstract: The malachite green assay is often used for ... Keywords: Malachite green, high throughput screening, pyrophosphatase, assay techniques, phosphate. ×. Restricted Access Panel ... Moreover, the assay is fully scalable from smaller drug screening efforts (˜ 20,000 wells per day) to ultra-high throughput ...
more infohttp://www.eurekaselect.com/70757