Search | Preprints.org
... high-throughput nucleotide sequencing, infiltrating ductal carcinoma (IDC), paraffin embedding, sequence alignment, ... Further, we explored high throughput epitranscriptome sequencing data to identify RNA chemical modifications in muscle-specific ... In y-c, an open reading frame (ORF) for 2600 nucleotides sequence, encoding 655 amino acids showed high homology with zebu ... We have recently developed a novel genome-wide experimental method, High-salt Recovered Sequences sequencing (HRS-seq), which ...
"High-throughput SNP discovery through deep resequencing of a reduced r" by David L. Hyten, Steven B Cannon et al.
... of the sequence scaffolds. The discovery and genetic mapping of more single nucleotide polymorphism (SNP) markers were needed ... To that end, next generation sequencing and high-throughput genotyping were combined to obtain a much higher resolution genetic ... We have demonstrated how next generation sequencing was combined with high-throughput SNP detection assays to quickly discover ... Those SNPs were then used to create a high resolution genetic map that assisted in the assembly of scaffolds from the 8× whole ...
Plasma contains ultrashort single-stranded DNA in addition to nucleosomal cell-free DNA<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 9% * Exons Medicine & Life Sciences 8% ... We introduce uscfDNA-seq, a single-stranded cell-free DNA next-generation sequencing pipeline, which bypasses previous ... We introduce uscfDNA-seq, a single-stranded cell-free DNA next-generation sequencing pipeline, which bypasses previous ... Patents regarding the extraction and sequencing of ultrashort single-stranded DNA have been filed. Funding Information: This ...
Development of Molecular Resources for an Intertidal Clam, Sinonovacula constricta, Using 454 Transcriptome Sequencing<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 54% * Single Nucleotide Polymorphism Medicine & Life Sciences 47 ... High-throughput deep sequencing of S. constricta using 454 pyrosequencing technology yielded 859,313 high-quality reads with an ... High-throughput deep sequencing of S. constricta using 454 pyrosequencing technology yielded 859,313 high-quality reads with an ... High-throughput deep sequencing of S. constricta using 454 pyrosequencing technology yielded 859,313 high-quality reads with an ...
Enhanced neoplasia detection in chronic ulcerative colitis: the ENDCaP-C diagnostic accuracy study<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 22% * ROC Curve Medicine & Life Sciences 19% ... To develop the DNA methylation test for high-throughput analysis within the NHS. To prospectively evaluate the test within the ... To develop the DNA methylation test for high-throughput analysis within the NHS. To prospectively evaluate the test within the ... To develop the DNA methylation test for high-throughput analysis within the NHS. To prospectively evaluate the test within the ...
Targeting a host-cell entry factor barricades antiviral-resistant HCV variants from on-therapy breakthrough in human-liver mice...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 13% View full fingerprint Cite this. * APA ... and the emergence of RAVs was monitored at high resolution using next-generation sequencing.RESULTS: HCV-infected human-liver ... and the emergence of RAVs was monitored at high resolution using next-generation sequencing.RESULTS: HCV-infected human-liver ... and the emergence of RAVs was monitored at high resolution using next-generation sequencing.RESULTS: HCV-infected human-liver ...
Prioritization and burden analysis of rare variants in 208 candidate genes suggest they do not play a major role in CAKUT<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 7% * Cluster Analysis Medicine & Life Sciences 6% ... The coding exons of 208 genes were analyzed in 453 patients with CAKUT using next-generation sequencing. Rare truncating, ... The coding exons of 208 genes were analyzed in 453 patients with CAKUT using next-generation sequencing. Rare truncating, ... The coding exons of 208 genes were analyzed in 453 patients with CAKUT using next-generation sequencing. Rare truncating, ...
A Bayesian Framework for Inferring the Influence of Sequence Context on Point Mutations<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 58% * Deamination Medicine & Life Sciences 40% ... Ling, G., Miller, D., Nielsen, R., & Stern, A. (2020). A Bayesian Framework for Inferring the Influence of Sequence Context on ... Ling, G, Miller, D, Nielsen, R & Stern, A 2020, A Bayesian Framework for Inferring the Influence of Sequence Context on Point ... A Bayesian Framework for Inferring the Influence of Sequence Context on Point Mutations. In: Molecular Biology and Evolution. ...
Sequencing framework for the sensitive detection and precise mapping of defective interfering particle-associated deletions...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 20% * Artifacts Medicine & Life Sciences 10% ... Overall, this work provides a detailed roadmap for high-resolution profiling and analysis of DIP-associated sequences within ... Overall, this work provides a detailed roadmap for high-resolution profiling and analysis of DIP-associated sequences within ... Overall, this work provides a detailed roadmap for high-resolution profiling and analysis of DIP-associated sequences within ...
Evolución atípica de cáncer de colon de larga duración<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 52% * Pathology Medicine & Life Sciences 34% ... keywords = "Colon cancer, Next generation sequencing, Thyroid metastases",. author = "Patricia Cruz-Castellanos and Laura Guti ... the importance of multidisciplinary management of this pathology and the emerging need to establish next generation sequencing ... the importance of multidisciplinary management of this pathology and the emerging need to establish next generation sequencing ...
The future of DNA sequencing - Peter Rohde
... high throughput sequencing technologies, which allow an entire human genome to be sequenced for $10,000, by a couple of ... which are on the order of 50 nucleotides in length. Each of these fragments is independently sequenced, and can be sequenced in ... Several competing high throughput technologies have emerged, but they all operate in a similar fashion. Rather than sequencing ... In particular, sequenced data from present day sequencing technologies have quite high error rates. This makes it more ...
A composite genome approach to identify phylogenetically informative data from next-generation sequencing<...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 78% * Composite Mathematics 71% View full fingerprint ... Background: Improvements in sequencing technology now allow easy acquisition of large datasets; however, analyzing these data ... This method eliminates the need for expensive marker development in many studies by using whole genome shotgun sequence data ... This method eliminates the need for expensive marker development in many studies by using whole genome shotgun sequence data ...
Circulating Tumor DNA in Head and Neck Squamous Cell Carcinoma: Association with Metabolic Tumor Burden Determined with FDG-PET...
High-Throughput Nucleotide Sequencing Medicine & Life Sciences 33% * Therapeutics Medicine & Life Sciences 5% ... Conclusions: A high metabolic tumor burden based on FDG imaging is associated with a positive liquid biopsy and high maximum ... Conclusions: A high metabolic tumor burden based on FDG imaging is associated with a positive liquid biopsy and high maximum ... Conclusions: A high metabolic tumor burden based on FDG imaging is associated with a positive liquid biopsy and high maximum ...
Genome-wide identification and differential analysis of translational initiation. | Broad Institute
High-Throughput Nucleotide Sequencing. Genome, Human. Sequence Analysis, RNA. RNA, Long Noncoding ... In November 2022, Broads Genomics Platform sequenced its 500,000th whole human genome, a mere four years after sequencing its ... Every summer, 18 high school students spend six weeks at Broad working side-by-side with mentors on cutting-edge research. ... The development of the translation initiation (TI) sequencing (TI-seq) technique has enabled the global mapping of TIs and ...
PRIME PubMed | Interpreting Mixture Profiles: Comparison between Precision ID GlobalFiler™ NGS STR Panel v2 and Traditional...
Body RemainsDNADNA FingerprintingForensic GeneticsGenetic MarkersHigh-Throughput Nucleotide SequencingHumansMicrosatellite ... Forensic genetic analysis of single-nucleotide polymorphisms and microhaplotypes in Koreans through next-generation sequencing ... Massively parallel sequencing of forensic STRs and SNPs using the Illumina® ForenSeq™ DNA Signature Prep Kit on the MiSeq FGx™ ... Massively parallel sequencing of 32 forensic markers using the Precision ID GlobalFiler™ NGS STR Panel and the Ion PGM™ System. ...
Type 2 innate lymphoid cells in the induction and resolution of tissue inflammation. | Broad Institute
High-Throughput Nucleotide Sequencing. Single-Cell Analysis. Immunity, Innate. Inflammation. Lymphocyte Activation ... In November 2022, Broads Genomics Platform sequenced its 500,000th whole human genome, a mere four years after sequencing its ... Every summer, 18 high school students spend six weeks at Broad working side-by-side with mentors on cutting-edge research. ... Student opportunities Learn about Broad Institutes mentored research offerings for high school students, college students, and ...
How it Works | Advanced Molecular Detection (AMD) | CDC
... and make high-throughput WGS possible. Newer sequencing platforms have revolutionized this field, by generating larger volumes ... Whole-genome sequencing (WGS) is a laboratory procedure that determines the order of all or most of the nucleotides in the ... These sequences are composed of chemical building blocks known as nucleotide bases. Determining the order of bases-"genomic ... Next-generation sequencing (NGS) refers to sequencing technologies that can process a large quantity of genetic material at a ...
How to Generate High-Quality SNPs Data Sets from NGS
SNPs can be identified in the large amounts of DNA sequence that is generated. The identification of SNPs in such data relies ... Now with the availability of high throughput sequencing methods, also known as next generation sequencing (NGS), SNPs can be ... SNPs or single nucleotide polymorphisms are on many scientists wish list in experimental studies of genomic DNA sequences. ... With high coverage sequencing data, you could simply ignore the low-quality alleles and count only the high-quality alleles ...
Assembly complexity of prokaryotic genomes using short reads | BMC Bioinformatics | Full Text
Nevertheless, these limitations are mitigated by much higher throughput (approximately 4 hours per 500 × 106 nucleotides of DNA ... Sequences and annotations. Sequence and annotation data for 384 completely sequenced bacterial and archaeal genomes were ... Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, et al.: Genome sequencing in microfrabricated high-density picolitre ... Given these results, we should expect assemblies to contain the complete sequences of a high fraction of protein-coding genes. ...
OPUS 4 | Search
Here we developed a luciferase reporter-based assay suitable to monitor siRNA off-targeting in a high throughput manner using ... To explore a potential role for circRNAs in epilepsy, RNA-sequencing (RNA-seq) was performed on hippocampal tissue from a rat ... We investigated the impact of chemically modifying single nucleotide positions within the siRNA seed on siRNA function and off- ... A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and ...
Search | Preprints.org
... generated by the combination of two high throughput sequencing platforms, Illumina short-read sequencing and PacBio Single ... We were able to differentiate between "healthy nucleotide sequences and disrupted sequences that may be associated with ... Although the LmjF genome sequence was done following a shotgun strategy and using classical Sanger sequencing, the results were ... Molecular Real-Time (SMRT) sequencing, which provides long-read sequences. Apart from resolving uncertain nucleotide positions ...
Mutational spectrum of adult T-ALL | Oncotarget
... we performed targeted high throughput sequencing of 88 candidate genes in 81 T-ALL samples of adult patients. ... Single nucleotide variations and short indels in adult T-ALL. We obtained an average of 1.2 million reads for each sample ... Whole-exome sequencing in adult ETP-ALL reveals a high rate of DNMT3A mutations, Blood. 2013; 121: 4749-4752. ... Clinical and molecular characterization of early T-cell precursor leukemia: a high-risk subgroup in adult T-ALL with a high ...
IOS Press Ebooks - GenBank: The NCBI Nucleotide Sequence Database
Bulk submissions of Expressed Sequence Tag (EST), Sequence Tagged Site (STS), Genome Survey Sequence (GSS), and High-Throughput ... The GenBank sequence database is an annotated collection of all publicly available nucleotide sequences and their protein ... Release 142, produced in June 2004, contained over 40.3 billion nucleotide bases in more than 35.5 million sequences. GenBank ... Upon receipt of a sequence submission, the GenBank staff assigns an Accession number to the sequence and performs quality ...
Aciphex for sale - Canadian pharmacy
High-throughput sequencing data is available from European Nucleotide Archive; accession number PRJEB55647. However, we also ... Overall, our results provide evidence that both dendritic spine parameters were determined by high-throughput sequencing ... A 48-hour aciphex for sale sequencing run was performed using E. RNAs that match 2 non-essential (nin204 and nin146) and 2 ... M MMV291 aciphex for sale was confirmed by sequencing. Protein expression was controlled by a seed mismatch, we isolated 2 ...
Scientific Program | Biocuration 2017 | Stanford Medicine
Abstract: With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are ... CDD can be searched with protein or nucleotide query sequences via NCBIs CD-Search interface, which utilizes RPS-BLAST, a ... Abstract: Considering higher throughput and lower costs, large-scale sequencing projects based on population genomics and ... High-throughput sequencing technologies provide a revolutionary way for transcriptome profiling, enable facile generation of ...
Dr. Alanna C. Morrison - Directory - MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
To be competitive in this fast-moving and high-throughput environment, my research group focuses on the application of ... millions of single nucleotide polymorphisms across the entire genome and evaluates large-scale population-based sequencing data ...
Clonal heterogeneity of FLT3 -ITD detected by high-throughput amplicon sequencing correlates with adverse prognosis in acute...
Furthermore, the 5′-UTR region (82 nucleotides), included in the FLT3 cDNA reference sequence, were substracted from the ... High-throughput FLT3-ITD amplicon sequencing (HTAS) and genomic targeted sequencing. In case of HTAS, FLT3 amplicons were ... To overcome this limitation, we performed cDNA-based high-throughput amplicon sequencing (HTAS) in 250 FLT3-ITD positive AML ... Therefore, we established a high-throughput FLT3-ITD amplicon sequencing assay to gain information complementary to the results ...
Search NCBI databases - NLM
Genome sequencing projects by organism. Nucleotide DNA and RNA sequences. SRA High-throughput sequence reads ... Protein sequences, 3-D structures, and tools for the study of functional protein domains and active sites ... Gene sequences and annotations used as references for the study of orthologs structure, expression, and evolution ... Genome sequence assemblies, large-scale functional genomics data, and source biological samples ...