Pathogens | Free Full-Text | Animal Models of Chronic Hepatitis Delta Virus Infection Host-Virus Immunologic Interactions
Animal models like hepadnavirus-infected chimpanzees or the eastern woodchuck have been of great value for the characterization ... defective RNA virus that has an absolute requirement for a virus belonging to the hepadnaviridae family like hepatitis B virus ... Recently, more practical animal models in which to perform a deeper study of host virus interactions and to evaluate new ... and fulminant hepatitis. Importantly, no selective therapies are available for HDV infection. The mainstay of treatment for HDV ...http://www.mdpi.com/2076-0817/4/1/46
Emergence of Drug-Resistant Populations of Woodchuck Hepatitis Virus in Woodchucks Treated with the Antiviral Nucleoside...
In vitro infection of woodchuck hepatocytes with woodchuck hepatitis virus and ground squirrel hepatitis virus. Virology. 1989; ... Emergence of Drug-Resistant Populations of Woodchuck Hepatitis Virus in Woodchucks Treated with the Antiviral Nucleoside ... reduces woodchuck hepatitis virus (WHV) titers in the sera of chronically infected woodchucks by inhibiting viral DNA synthesis ... 5. Cohen J I, Miller R H, Rosenblum B, Denniston K, Gerin J L, Purcell R H. Sequence comparison of woodchuck hepatitis virus ...http://pubmedcentralcanada.ca/pmcc/articles/PMC89396/?lang=en-ca
Enhancing Transgene Expression from Recombinant AAV8 Vectors in Different Tissues Using Woodchuck Hepatitis Virus Post...
Therefore, we applied the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) to enhance AAV8-mediated ... Enhancing Transgene Expression from Recombinant AAV8 Vectors in Different Tissues Using Woodchuck Hepatitis Virus Post- ... Enhancing Transgene Expression from Recombinant AAV8 Vectors in Different Tissues Using Woodchuck Hepatitis Virus Post- ... Adeno-associated virus (AAV) vectors have been utilized extensively in gene therapy and gene function studies, as strong ...http://www.medsci.org/v13p0286
"Hepatocellular Carcinoma in the Woodchuck Model of Hepatitis B Virus " by Bud C. Tennant, Ilia A. Toshkov et al.
Woodchuck hepatitis virus [WHV]) that is similar in structure and replicative life cycle to the human hepatitis B virus (HBV). ... Like HBV, WHV infects the liver and can cause acute and chronic hepatitis. Furthermore, chronic WHV infection in woodchucks ... The woodchuck model has been important in the preclinical evaluation of safety and efficacy of the antiviral drugs now in use ... The woodchuck model of viral induced HCC has been used effectively for the development of new imaging agents for enhancement of ...http://digitalcommons.unl.edu/veterans/79/
IFN-alpha gene therapy for woodchuck hepatitis with adeno-associated virus: differences in duration of gene expression and...
IFN-alpha gene therapy for woodchuck hepatitis with adeno-associated virus: differences in duration of gene expression and ... encoding woodchuck IFN-alpha (AAV-IFN) to treat animals with chronic woodchuck hepatitis virus infection. ... IFN-alpha gene therapy for woodchuck hepatitis with adeno-associated virus: differences in duration of gene expression and ... IFN-alpha gene therapy for woodchuck hepatitis with adeno-associated virus: differences in duration of gene expression and ...https://www.cun.es/en/research/scientific-publications/ifn-alpha-gene-therapy-woodchuck-hepatitis-adeno-associated-virus
Validation-based insertional mutagenesis identifies lysine demethylase FBXL11 as a negative regulator of NFκB | PNAS
... woodchuck hepatitis virus posttranscriptional regulatory element. ... Virus Production, Cell Infection and Selection.. The VBIM and ... To determine the titer of each VBIM virus, 293T cells were infected with serially diluted virus and analyzed for GFP expression ... LTR during virus packaging. This important design feature allows in virus titers that are comparable to those obtained with ... 3A, right panel). In total, about 3 × 106 Z3 cells were infected with the three VBIM viruses and selected in GCV for ...https://www.pnas.org/content/106/38/16339
Using the transcription factor inhibitor of DNA binding 1 to selectively target endothelial progenitor cells offers novel...
cPPT, central polypurine tract; WPRE, woodchuck hepatitis virus post transcription regulatory element; LTR, long terminal ...https://www.ncbi.nlm.nih.gov/pubmed/20807818
CAG, CMV enhancer/β-globin chimeric promoter; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; ITR, ... 2000) Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors. J Virol 74:2777-2785. ... D, E, Similar to B and C; in this case, agrp-cre;rosa26-loxSTOPlox-eyfp mice were used with rAAV-FLEX-rev-ChR2mCherry virus ... Also, because the virus is targeted by injection to a spatially defined subset of neurons, the origin of labeled projections is ...http://www.jneurosci.org/content/28/28/7025
JCI - Lentivector-mediated RNAi efficiently suppresses prion protein and prolongs survival of scrapie-infected mice
Ψ, packaging signal; ppt, polypurine tract; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; triangle, ...https://www.jci.org/articles/view/29236/figure/1
Molecular therapy for obesity and diabetes based on a long-term increase in hepatic fatty-acid oxidation - Orellana-Gavaldà -...
Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J Virol 1998; 72: 5085-5092.. *PubMed, ... Berns KI, Linden RM. The cryptic life style of adeno-associated virus. Bioessays 1995; 17: 237-245.. Direct Link: ... Insect cells as factory to produce adeno-associated virus type 2 vectors. Hum Gene Ther 2002; 13: 1935-1943.. *CrossRef, ...http://onlinelibrary.wiley.com/doi/10.1002/hep.24140/references
Suppression of angiogenesis by lentiviral delivery of PEX, a noncatalytic fragment of matrix metalloproteinase 2 | PNAS
W, posttranscriptional regulatory element of woodchuck hepatitis virus (yellow); ppt, polypurine tract. (B) Analysis of PEX ... a posttranscriptional regulatory element of the woodchuck hepatitis virus was included (Fig. 1A) that enhances expression of ... All viruses were pseudotyped with the G protein of the vesicular stomatitis virus (VSV-G) which allows the infection of broad ... Virus Production.. Lentiviral vectors were produced by using a four-plasmid, third-generation, Tat-free packaging system (26). ...https://www.pnas.org/content/97/22/12227
JCI - TALE-mediated epigenetic suppression of CDKN2A increases replication in human fibroblasts
jTALE, jumbled TALE; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element. (C) Percent DNA methylation of ... The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) present in lentiviral constructs was used as a ...https://www.jci.org/articles/view/77321
Novel macromolecular inhibitors of human immunodeficiency virus-1 protease. - PubMed - NCBI
Woodchuck hepatitis virus post-transcriptional regulatory element; CMV: human cytomegalovirus immediate-early promoter; RSV: ... Novel macromolecular inhibitors of human immunodeficiency virus-1 protease.. Miklóssy G1, Tözsér J, Kádas J, Ishima R, Louis JM ... While the recombinant virus containing the PR(RE) sequence showed only very low level of expression, expression of the viral ... An intracellularly expressed defective human immunodeficiency virus type-1 (HIV-1) protease (PR) monomer could function as a ...https://www.ncbi.nlm.nih.gov/pubmed/18480092
Frontiers | Optogenetic Control of Neural Circuits in the Mongolian Gerbil | Frontiers in Cellular Neuroscience
Here, we have established a reliable, gene delivery system using AAV8(Y337F)-pseudotyped recombinant adeno-associated virus ( ... Here, we have established a reliable, gene delivery system using AAV8(Y337F)-pseudotyped recombinant adeno-associated virus ( ... woodchuck hepatitis virus posttranscriptional regulatory element. ... AAV, adeno-associated virus; AP, action potential; CatCH, Ca2+- ... For instance, about half of MNTB neurons within the target area were transduced by a single virus injection of 250 nl (n = 2). ...https://www.frontiersin.org/articles/10.3389/fncel.2018.00111/full
Genetic Modification is Not the Same As What People Have Been Doing for Thousands of Years
They were from woodchuck hepatitis virus; mouse enzyme; jellyfish fluorescence; tetracycline transactivator; mouse ... cytomegalovirus; Gibbon ape leukemia virus; Moloney mouse leukemia virus; and vesicular stomatitis virus. ...https://anh-usa.org/genetic-modification-is-not-the-same-as-what-people-have-been-doing-for-thousands-of-years/
Hepatitis B virus C-gene variants | Springer for Research & Development
The heterogeneity of hepatitis B virus (HBV) is increasingly believed to play a role in viral persistence, pathogenesis, and ... Fulminant Hepatitis Chronic Carrier Woodchuck Hepatitis Virus Encapsidation Signal Initiation Codon Mutation These keywords ... Cellular immune response to hepatitis B virus- encoded antigens in acute and chronic hepatitis B virus infection. J Immunol 145 ... Miska S., Will H. (1993) Hepatitis B virus C-gene variants. In: Gerlich W.H. (eds) Research in Chronic Viral Hepatitis. ...https://rd.springer.com/chapter/10.1007/978-3-7091-9312-9_16
Occult lifelong persistence of infectious hepadnavirus and residual liver inflammation in woodchucks convalescent from acute...
A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A 1978 ... Woodchuck hepatitis virus is more efficient oncogenic agent than ground squirrel hepatitis virus in a common host. J Virol 1991 ... of woodchuck hepatitis virus surface antigen into hepatocyte plasma membrane in woodchuck hepatitis and in the virus-induced ... Hepatocellular carcinoma in woodchuck hepatitis virus-infected woodchucks: presence of viral DNA in tumor tissue from chronic ...http://onlinelibrary.wiley.com/doi/10.1002/hep.510290329/references?globalMessage=0
WPRE is the woodchuck hepatitis virus posttranscriptional regulatory element (Madisen et al., 2012; Hochbaum et al., 2014). Red ... Adeno-associated virus vectors can drive high expression in vivo, but typically show nonuniform expression that is highest near ... and requires expression via a rabies virus. Another fast GFP-based GEVI, ASAP2f, was used recently to investigate subcellular ...http://www.jneurosci.org/content/36/43/11059
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse | eLife
... the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and a neomycin resistance gene (NeoR), all flanked ... woodchuck hepatitis virus post-transcriptional regulatory element; pA: bovine growth hormone polyadenylation sequence; NeoR: ... CD4 mRNA expression in CD19-positive B cells and its suppression by the Epstein-Barr virus * RD Zhang ... These signs are antigens - processed pieces of proteins from invading bacteria and viruses - which are displayed on the surface ...https://elifesciences.org/articles/32417
A mutant Tat protein inhibits infection of human cells by strains from diverse HIV-1 subtypes | springermedizin.de
Rous Sarcoma Virus (RSV) promoter is used to transcribe mRNA for packaging in VLPs. The woodchuck hepatitis virus post ... Clade homogeneity and low rate of delta virus despite hyperendemicity of hepatitis B virus in Ethiopia. ... Mbita Z, Hull R, Dlamini Z. Human immunodeficiency virus-1 (HIV-1)-mediated apoptosis: new therapeutic targets. Viruses. 2014;6 ... NB-ZSG1 inhibits virus replication of all the HIV-1 strains tested in primary CD4+ T cells. CD4+ T cells (NT) or CD4+ T cells ...https://www.springermedizin.de/a-mutant-tat-protein-inhibits-infection-of-human-cells-by-strain/12139016?fulltextView=true
Lentiviral Strategies for RNAi Knockdown of Neuronal Genes - Current Protocols
Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J. Virol. 72:5085‐5092. ... Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral ... Support Protocol 3: Concentration of VSV/G‐Pseudotyped Virus by Ultracentrifugation. Materials * Virus‐transfected 293T cells ... Support Protocol 2: Production of shRNA‐Expressing Virus. *Support Protocol 3: Concentration of VSV/G‐Pseudotyped Virus by ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ns0526.html
New alleles for complex cell labeling
Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE), and poly(A) posttranscriptional sequences. In this ...https://www.jax.org/news-and-insights/2015/december/new-alleles-for-complex-cell-labeling
rAVE™ @ Q l ^ x N ^ [ ڍ?? | R X E o C I ??
Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J Virol. 1998 72(6):5085-92. ... Genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins B and D. J Virol ... A novel recombinant adeno-associated virus vaccine induces a long-term humoral immune response to human immunodeficiency virus ... Adeno-associated virus type 5 (AAV5) but not AAV2 binds to the apical surfaces of airway epithelia and facilitates gene ...http://www.cosmobio.co.jp/support/technology/a/products_gdc_20030512_01.asp
Prodrugs for Liver-targeted Drug Delivery | Springer for Research & Development
OHexadecylpropanediol-3-Phosphoacyclovir and Acyclovir in Woodchucks with Chronic Woodchuck Hepatitis Virus Infection. ... Bile Acid Adefovir Dipivoxil Vinyl Ketone Bile Acid Transporter Woodchuck Hepatitis Virus These keywords were added by machine ... Enhanced Antiviral Activity of Phosphatidyl-dideoxyguanosine versus Dideoxyguanosine in Woodchuck Hepatitis Virus Infection In ... Metabolic sSudies on BMS-200475, A New Antiviral Compound Active against Hepatitis B Virus. Antimicrob Agents Chemother 1999; ...https://rd.springer.com/chapter/10.1007/978-0-387-49785-3_16
Addgene: Search results
... woodchuck hepatitis virus ... EGFP-ires-tTA Aequorea victoria, Encephalomyocarditis virus, E ... CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE Chicken; CMV, P1 phage, SV40 , Entacmaea quadricolor, Encephalomyocarditis virus, E.coli, ...https://www.addgene.org/search/advanced/?q=SuperNova%20Green