HCT116 is a human colon cancer cell line used in therapeutic research and drug screenings. HCT116 cells have a mutation in ... it was found that the knockout of MARCH2 limited growth of HCT116 cells via stress on the endoplasmic reticulum. HCT116 cells ... HCT116 cells can also function in xenografts, with docetaxel, 5-FU, and flavopiridol limiting tumor growth in vitro. The HCT116 ... HCT116 cells remain arrested in the G1 phase. The proliferation of HCT116 colonies was found to be inhibited by 5-Fu/P85 ...
Cell Line Lysates* Close. *. Image. ALiCE® is a radically different approach to cell-free expression and a kit unlike any ... Overexpression FFPE Cell Line Sections* Close. *. Image. ALiCE® is a radically different approach to cell-free expression and a ... Cell Lines* Close. *. Image. The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any ... Stem Cells* Close. *. Image. The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any ...
In this study, we demonstrate that carnosine inhibits the proliferation of human HCT116 colon cancer cells. In this cell line, ... In this study, we demonstrate that carnosine inhibits the proliferation of human HCT116 colon cancer cells. In this cell line, ... In HCT116 cells these effects are related to decreased ERK1/2 phosphorylation and increased p21waf1 protein. Our findings ... Carnosine inhibits KRAS-mediated HCT116 proliferation by affecting ATP and ROS production Cancer Lett. 2012 Feb 28;315(2):122-8 ...
This is a poster presented at AACR 2018 that explores the use of reporter cell lines in drug discovery and development. ... HCT116 VIM RFP In vitro cell model for dissecting the molecular switches underlying EMT and for identifying compounds that ... cell line, which is one of the most widely used and recognized lung cancer cell lines. The A549 VIM RFP cell line harbors a C- ... The EMT reporter cell line was verified at the nucleic acid (genomic and mRNA) and protein levels and functional levels by cell ...
Crocetin suppresses the growth and migration in HCT-116 human colorectal cancer cells.Nov 30, 2020. ... Crocetin protects against mitochondrial damage induced by UV-A irradiation in corneal epithelial cell line HCE-T cells.Nov 30, ... Crocetin may increase autophagic cell death in fluorouracil-treated breast cancer cells.Feb 28, 2017. ... Anti-metastatic properties of a potent herbal combination in cell and mice models of triple negative breast cancer.Feb 14, 2020 ...
IL-8 KO cell line available now. KO validated by Next Generation Sequencing (NGS), Western Blot (WB). Free of charge wild type ... Lane 5 : A431 cell lysate. Lane 6 : HCT116 cell lysate. Lysates/proteins at 30 µg per lane.. Performed under reducing ... Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
subtype 3 triggers higher proliferation of human colorectal cancer cells, HCT116. Parasitol Res. 2013;112:3551-5. DOIPubMed ... We observed infiltration by numerous neutrophils, eosinophils, plasma cells, and lymphocytes through all layers and into the ... a substantial inflammatory reaction and an increased propagation of human colorectal cancer cells exposed to Blastocystis ST3 ...
HCT116 human colon cancer cells and METTL14. GSE188731. Time-regulated transcripts with the potential to modulate human ... miRNA transcriptome profiling of spheroid-enriched cells with cancer stem cell properties in human breast MCF-7 cell line.. ... Analysis of microRNA expression through microarray scan in HCT116 human colon cancer cells with or without METTL14 ... Expression data from HaCaT cells stimulated by IL-22. GSE73598. Expression data from cardiac stem cells (CSCs) with or without ...
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 10% SDS PAGE GTX100608 diluted at 1:1000 ... PLoS One 2015; 10 (7) : e0126566 molecular mechanism of the cell death induced by the histone deacetylase pan inhibitor lbh589 ... PLoS One 2015; 10 (7):e0126566 molecular mechanism of the cell death induced by the histone deacetylase pan inhibitor lbh589 ...
Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell ... Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells ... Based on these cancer cell features we can propose laser impulse strategy of killing cancer cells where initial impulse(s) may ... We using in vitro and in silico approaches revealed that main impact on the cell oxidative state makes cascade of secondary ...
... of NF-kappa B responses by epigenetic suppression of I kappa B alpha expression in HCT116 intestinal epithelial cells. American ... Tsukamoto H, Zhu NL, Asahina K, Mann DA, Mann J. Epigenetic cell fate regulation of hepatic stellate cells. In: Hepatology ... pancreatic stellate cells induce glycolysis in cancer cells. In: Society of Academic and Research Surgery 2016 Annual Meeting. ... Cell Death and Differentiation 2007, 14(2), 275-285. * Smart DE, Green K, Oakley F, Weitzman JB, Yaniv M, Reynolds G, Mann J, ...
The results show that AT dose-dependently inhibited degranulation in C48/80-stimulated RBL-2H3 cells by inhibiting β-Hex and ... and the diameter of Evans Blue extravasation and significantly ameliorate pathological changes and mast cell degranulation in ... and F-actin microfilament staining were used to evaluate the inhibitory effect of AT in RBL-2H3 cells stimulated with Compound ... Transcriptome Profiling of HCT-116 Colorectal Cancer Cells with RNA Sequencing Reveals Novel Targets for Polyphenol Nano ...
Cell culture. The four human CRC cell lines (DLD-1, SW1116, SW480 and HCT116) and a normal colon epithelial cell line (FHC) ... DLD-1, SW480 or HCT116 cells were plated in 6-well plates at a density of 3×105 cells per well and cell transfection was ... Transwell assay was performed to assess (C) cell migration and (D) invasion in transfected DLD-1 and SW480 cells. HCT116 cells ... 4I) in HCT116 cells. These data indicated that AQP1 is the target of miR-133a-3p and played a role in CRC cell proliferation, ...
Retroviral vector pSEB61 was retrofitted in established HCT116-siKCN and SW480-siKCN cells to silence KCNQ1OT1. Cellular ... proliferation was measured using CCK8 assay, and flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) ...
To investigate the potentiation effect of Genipin to Cisplatin induced cell senescence in HCT-116 colon cancer cells in vitro. ... Cells treated with Cisplatin alone or combined with Genipin, ROS negatively, while MMP positively correlated with cell ... A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells. Int J Med Sci. 13( ... A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells Ruihua Wang1, KC ...
5 TABLE 5 Cell Lines Origin Cell line Colorectal adenocarcinoma HCT-116 Colorectal adenocarcinoma LS174T Leukemia HL60 Mammary ... lsqb;0245] Table 5 below sets forth the cell lines utilized in the above described cell proliferation assay. For each cell ... Colon cancer cells (COLO-205 cell line) were added to a 96 well plate at a seeding density of 1.25×104 cells/well/150 &mgr;L. A ... Cells from normal tissues and a wide variety of different tumour types were chosen for analysis. Many of the tumour cells were ...
Cell lines and cell culture. Human colorectal cancer cells HCT116, SW116, LoVo, SW480 and Caco2 were preserved in our institute ... 3D). We then examined cell-cycle distribution in GRHL2-over-expressed cells. The percentage of cells in S phase and G2/M phase ... Cell proliferation assay. Cell proliferation assays were performed using a Cell Counting Kit-8 (CCK-8; cat.CK04, DOJINDO, ... Regulation of the hTERT promoter activity by MSH2, the hnRNPs K and D, and GRHL2 in human oral squamous cell carcinoma cells. ...
... as revealed by real-time monitoring using HCT116 cells.. CONCLUSIONS: Evolutionarily conserved high-abundance miRNAs are ... Computational modeling highlighted the stem-cell marker Krüppel-like factor 4 (KLF4) as a potential target of miR-206. In a ... Forced expression of miR-206 resulted in significantly increased cell proliferation kinetics, ... which was further supported by miR-206 knockdown and ectopic upregulation in human colon cancer cells. ...
CpG methylation status in normal cells points to locally active regulatory sites at super-enhancers, which are targeted by ... we identify a type of far-reaching DNA methylation alteration in cancer cells of the distal regulatory sequences described as ... Therefore, we treated the colorectal cancer cell lines HCT116 and SW1116 at sub-lethal concentrations with the BET-bromodomain ... In the lung tumorigenesis samples from the H1437 (lung adenocarcinoma) and H157 (lung squamous cell carcinoma) cancer cell ...
We assure the cell line is free of mycoplasma and with good viability. Free technical guidance e.g. tips for cell culture will ... This cell line is validated by Sanger sequencing. A validation report and STR authentication report will be provided. ... The TMCO1 Knockout cell line (293T) is constructed based on (1) the optimal design of the Red Cotton System, (2) CRISPR-U™ ... TMCO1 Knockout cell line (HCT 116) inquiry More details Organism: Human. Gene Name: TMCO1 ...
... induces apoptosis via regulation of mitochondria-endoplasmic reticulum interaction in HCT116 human colorectal cancer cells. ... For the first time we demonstrated that stabilin-1 ectopically expressed in CHO cells mediates endocytic uptake of EGF, key ... 1149 - Reactive oxygen species induced by OSU-A9 inhibit the growth of duodenal cancer and gastric cancer cells through ... 782 - Macrophage-cancer cell fusion is mediated by Phosphatidylserine-CD36 receptor interaction and induced by ionizing ...
"In vitro Cytotoxic Activity of Pediococcus acidilactici S3 Cell Free Supernatant Against Colorectal Cancer Cells (HCT 116)"; Mr ... "Partial Purification of Philippine Plant M004 Components and Evaluation of Anticancer Potential Against 2 Breast Cancer Cell ...
This process is key to a number of cellular functions such as signal recognition and cell adhesion. ... Choose from over 300 knock-in and knockout cell line models in many standard cancer cell lines such as DLD1, MCF10A, and HCT116 ... Human knockout HAP-1 cells The single largest bank of isogenic cell lines with over 7,500 cell lines to choose from and trusted ... Explore 免疫細胞スクリーニングサービス CRISPR immune cell screens Custom immune cell screens Compound screening assays iTreg誘導アッセイ iTreg抑制アッセイ ...
Zerumbone reduces proliferation of HCT116 colon cancer cells by inhibition of TNF-alpha. SP Singh, K Nongalleima, NI Singh, P ...
SN exhibited cytotoxic effects in human CRC RKO and HCT-116 cells in vitro and in vivo. It inhibits their proliferation, ... The cell cycle of healthy cells stops at the right time. New, young cells replace stem cells, which age and undergo apoptosis. ... SMM7721 cells, and HepG2 cells. It inhibits proliferation and induces apoptosis and cell cycle arrest in the G2/M phase. SM ... DNMT1 is an enzyme that regulates cell survival, cell cycle arrest, and cell death, and c-Jun is a subunit of activating ...
... via a period reliant way in HCT-116 cells (Extra document 1: Amount Beds2C). Jointly, our outcomes revealed that account ... we attenuated G-1 activated ROS era by pretreatment cells with ROS GRK4 scavenger NAC for 1?l (Fig.?4b). The cell viability lab ... uncovered that G-1 considerably elevated the ROS era via a dosage of reliant good manners in both HCT-116 and SW480 cells (Fig ... The potentially oncogenic Epstein-Barr virus (EBV) is carried by almost all During T cell account activation, the DNA lesions ...
Both diastereomers were tested for their antiproliferative activities in HCT116 cells, showing that natural (30R)-apratoxin E ...
HCT116 cells) effect. However, in HCT116 cells IKKα was the dominant IKK required for basal phosphorylation of p65 at S536, ... Here we show that suboptimal T cell receptor stimulation of CD8 T cells upregulates FOXP3 in vitro. Similarly, CD8 T cells ... Cell-surface proteins provide excellent biomarkers to identify specific cell types and resolve heterogeneous cell populations. ... Cell-intrinsic loss of FOXP3 by CD8 T cells resulted in improved functionality after TCR stimulation and better antitumor ...
Description: Human BLM (-/-) HCT116 Cell line is Homozygous deletion of exon 5 and partial deletion of exon 6 ... Description: Human BLM (-/-) HCT116 Cell line is Homozygous deletion of exon 5 and partial deletion of exon 6 ... This is a quasidiploid human cell line with the modal number 46 occurring in 76% of cells (range = 41 to 47 for 50 metaphases). ... The Covcheck.Hctx reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these ...
RPMI-8226 and HCT-116) and 8b, 9a, and 9e on the HCT-116 cell line. The antiproliferative activity was determined for the ... Notably, the HepG2 hepatoma cell line was the most frequently employed liver cell type, despite acknowledged limitations. ... chalcone series on three cell lines: RPMI-8226, HCT-116, and MCF-7. Compounds 8b, 9a, 9b, and 9f were the most active ones. To ... Both compounds 8b and 9b were selected for cell cycle analysis and apoptotic assays. Moreover, molecular docking results of the ...