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*  Selectively Enhanced Cellular Signaling by Gi Proteins in Essential Hypertension | Circulation Research
guanosine 5′-[γ-thio]triphosphate. HT, NT cells. =. cell lines established from hypertensive and normotensive subjects. ... Basal IP3 levels were not different in NT and HT fibroblasts. Values are mean±SD from each of the four NT and HT cell lines. *P ... The rabbit polyclonal antibodies directed against peptide epitopes on Gαi/o/t/z, Gβ1, Gβ2, Gβ3, and Gβ4 were purchased from St ... Figure 3. SPP-, thrombin (Thr)-, angiotensin II (Ang II)-, and ET-1-evoked [Ca2+]i signals in NT and HT fibroblasts. Skin ...
http://circres.ahajournals.org/content/79/5/974
*  Riobo NA[au] - PubMed - NCBI
Evaluating the Activity of Smoothened Toward G Proteins Using [³⁵S]Guanosine 5'-(3-O-thio)triphosphate ([³⁵S]GTPγS). ... Phosphoinositide 3-kinase and Akt are essential for Sonic Hedgehog signaling.. Riobó NA, Lu K, Ai X, Haines GM, Emerson CP Jr. ... 2011 Jun 3;286(22):19589-96. doi: 10.1074/jbc.M110.197111. Epub 2011 Apr 7. ... 2013 Mar;83(3):691-7. doi: 10.1124/mol.112.082511. Epub 2013 Jan 4. ...
https://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Riobo+NA%5Bau%5D&dispmax=50
*  Tim Stearns | Stanford Medicine Profiles
Asp26 (homologous to Gly12 of p21ras) was essential for the binding of the activating nucleotide, guanosine 5'-3-O-(thio) ... The cell center at 100 CELL Stearns, T., Winey, M. 1997; 91 (3): 303-309 View details for Web of Science ID A1997YD94100004 ... FOP Is a Centriolar Satellite Protein Involved in Ciliogenesis PLOS ONE Lee, J. Y., Stearns, T. 2013; 8 (3) Abstract. ... GREEN FLUORESCENT PROTEIN - THE GREEN-REVOLUTION CURRENT BIOLOGY Stearns, T. 1995; 5 (3): 262-264 Abstract. Green fluorescent ...
https://med.stanford.edu/profiles/tim-stearns
*  Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein | PNAS
1992) A truncated recombinant alpha subunit of Gi3 with a reduced affinity for beta gamma dimers and altered guanosine 5′-3-O-( ... γ-thio]triphosphate (GTPγS) had a more marked effect (∼ +20 °C; Fig. S1). The larger extent of the stabilization upon GTPγS ... whereas addition of the same concentration of the nonhydrolyzable GTP analog guanosine 5′-O-[ ... 3. GBAi does not bind to Gαi effectors or the Gαi regulators RGS GAPs, GoLoco GDIs, and Ric-8A. (A) GBAi does not bind to the ...
http://www.pnas.org/content/114/48/E10319
*  A Novel Family of Potent Negative Allosteric Modulators of Group II Metabotropic Glutamate Receptors | Journal of Pharmacology...
Nonspecific binding was determined in the presence of 10 μM unlabeled guanosine 5′-3-O-(thio)triphosphate. The assay mixtures ... thio)triphosphate were purchased from Sigma-Aldrich, Inc., (St. Louis, MO). Unifilter-96 GF/B plates and MicroScint-20 were ... Probenecid, dimethyl sulfoxide (DMSO), puromycin dihydrochloride, GDP, and guanosine 5′-3-O-( ... guanosine 5′-O-(3-[35S]thio)triphosphate; ACSF, artificial cerebrospinal fluid; l-AP4, L(+)-2-amino-4-phosphonobutyric acid; ...
http://jpet.aspetjournals.org/content/322/1/254.long
*  Plus it
GR231118- and rPP-stimulated guanosine 5′-3-O-(thio)triphosphate binding through hY4 receptors with significantly lower ... thio)triphosphate; DMEM, Dulbecco's modified Eagle's medium; HA, hemagglutinin; KH, Krebs-Henseleit; HEK, human embryonic ... 3. Pretreatment with GR231118 (GR) inhibits subsequent Y4 and Y1 but not Y2 agonist responses in WT (solid bars) and in Y1-/- ... PP, PYY, and NPY act through four Gi-coupled receptors (Y1, Y2, Y4, or Y5), with a fifth functional type (Y6) present only in ...
http://jpet.aspetjournals.org/content/319/1/20
*  Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding | Protocol (Translated to Dutch)
Guanosine trifosfaat (GTP) binding is een van de vroegste gebeurtenissen bij G-proteïne-koppelde receptor (GPCR) activatie. Dit ... Guanosine trifosfaat (GTP) binding is een van de vroegste gebeurtenissen bij G-proteïne-koppelde receptor (GPCR) activatie. Dit ... thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Mol. Pharmacol. 47, (4), 848-854 (1995). ... 35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS) Perkin Elmer. NEG030H. ...
https://www.jove.com/video/55561/het-meten-van-g-eiwit-gekoppelde-receptor-signaling-via-radio?language=Dutch
*  Peroxisome Biogenesis: Involvement of ARF and Coatomer | JCB
guanosine 5′-O-(2-thiodiphosphate). GTP-γS. guanosine 5′-O-(3-thiotriphosphate). Pmp. peroxisomal membrane protein. PTS. ... guanosine 5′-O-[3-thiotriphosphate]) or GDP-βS (guanosine 5′-O-[2-thiodiphosphate]) in a total volume of 3 ml essentially as ... and coatomer in a strictly guanosine 5′-O-(3-thiotriphosphate)-dependent manner. Consistent with this observation, ldlF cells ... 3. The size distribution of peroxisomes and the incorporation of new catalase. J Cell Biol 45:408-415, pmid:5513609.. ...
http://jcb.rupress.org/content/141/2/373
*  SMART: Secondary literature for PLDc domain
The enzyme was inhibited by oleate and activated by the small G proteins ARF3 and RhoA in the presence of guanosine 5'-3-O-( ... gamma-thio)triphosphate. Moreover, the effects of GTP gamma S and V(4+)-OOH were additive. Hence, activation of G proteins ... The stimulation of phospholipase D hydrolytic activity by GTP gamma S was inhibited by 2 mM guanosine 5'-(2-O-thiol)diphosphate ... GDP and its analogue, guanosine 5'-O-[beta-thio]diphosphate, inhibited the stimulatory effect of GTP[S], whereas the PMA ...
http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=PLDc
*  KAKEN - Research Projects | The molecular biollogical and electrophysiological investigation of the degranulation of human...
Publications] Toshiya Aizawa et al: 'Induction of granule release by intracellular applicaation of calcium and *guanosine-5_2-o ... Publications] Toshiya Aizawa: 'Induction of granule release by intracellular application of calcium and guanosine-5'-o-(3- ... thiotriphosphate) in human eosinophils.' J.Allergy Clin.Immunol.90. 789-795 (1992). *. Description. 「研究成果報告書概要(和文)」より ... Publications] Toshiya Aizawa: 'Induction of granule release by intracellular application of calcium and guanosine-5'-o-(3- ...
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04670454/
*  Plus it
... current activated in cells dialyzed with the nonhydrolyzable GTP analogue guanosine-5′-O-(3-thio)triphosphate. Pre-exposure to ... 3). In the presence of 1 μm prazosin, NE activated the Cl− current with an EC50 value of 5.7 ± 1.1 nm in PTX treated myocytes ( ... average of 5 to 11 experiments. The Cl−conductance (G Cl) measured at each concentration was normalized to G Clactivated by a ... 5A) or in cells where the Cl−current was activated by high concentrations of Iso (Fig. 5B) can be explained by an antagonistic ...
http://molpharm.aspetjournals.org/content/51/5/853
*  Plus it
guanosine-5′-O-(3-thio)triphosphate. CHO. Chinese hamster ovary. PMSF. phenylmethylsulfonyl fluoride. LPA. lysophosphatidic ... Using [35S]guanosine-5′-O-(3-thio)triphosphate (GTPγS) and radioligand bindings in insect Sf9-hCB2 membranes, we showed that ... guanosine-5′-O-(3-thio)triphosphate (GTPγS) exchange and inhibiting cAMP. Moreover, as a weak partial agonist at hCB2, ... IC50 values were converted to apparent Ki values by the method of Cheng and Prusoff (1973) with theKd values for [3H]CP55,940 ...
http://molpharm.aspetjournals.org/content/57/5/1045
*  Guanosine 5ʹ-O-(3-Thiotriphosphate), Tetralithium Salt - CAS 94825-44-2 - Calbiochem CAS 94825-44-2 | 371545
Guanosine 5ʹ-O-(3-Thiotriphosphate), Tetralithium Salt - CAS 94825-44-2 - Calbiochem CAS 94825-44-2 G-protein-activating GTP ... 371545 Guanosine 5ʹ-O-(3-Thiotriphosphate), Tetralithium Salt - CAS 94825-44-2 - Calbiochem. ... Guanosine 5ʹ-O-(3-Thiotriphosphate), Tetralithium Salt - CAS 94825-44-2 - Calbiochem ... Following reconstitution, aliquot and freeze (-20°C). Stock solutions lose about 5% of their activity after 6 months at -20°C. ...
http://www.emdmillipore.com/US/en/product/Guanosine-5-O-3-Thiotriphosphate-Tetralithium-Salt-CAS-94825-44-2-Calbiochem,EMD_BIO-371545
*  A model depicting gating of GIRK channels by the combin | Open-i
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology. *Heart Atria/chemistry/cytology. *Membrane Potentials/physiology ... getmorefigures.php?uid=PMC2230539&req=5. .flowplayer { width: px; height: px; } Figure 8: A model depicting gating of GIRK ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2230539_JGP8035.f8&req=4
*  Jeffrey S. Elmendorf, PhD
Elmendorf JS, Chen D, Pessin JE: Guanosine 5'-O(3-Thiotriphosphate) (GTPγS) stimulation of GLUT4 translocation is tyrosine ... Elmendorf JS, Damrau-Abney A, Smith TR, David TS, Turinsky J: Phosphatidylinositol 3-kinase and dynamics of insulin resistance ... Chen G, Raman P, Bhonagiri P, Strawbridge AB, Pattar G, Elmendorf JS: Protective effect of phosphatidylinositol 4,5- ... Strawbridge AB, Elmendorf JS: Phosphatidylinositol 4,5-bisphosphate reverses endothelin-1-induced insulin resistance. Diabetes ...
https://medicine.iu.edu/faculty/13333/jeffrey-s-elmendorf/
*  All systems go | JCB
Kinetic analysis of guanosine 5′-O-(3-thiotriphosphate) effects on phosphatidylinositol turnover in NRK cell homogenates. ... Figure 3. The behavior of a bead attached to a rotating flagellum (white circle, shown in three successive positions) can be ... 3). The study tied together the macroscopic output of an entire pathway with the fluctuations of an individual molecule, and ... They say that systems approaches will be so powerful that everyone will be using them routinely within 5-10 years. What ...
http://jcb.rupress.org/content/165/3/299
*  Pharmacokinetics and Pharmacodynamics of Cannabinoids | SpringerLink
Loss of cannabinoid-stimulated guanosine 5′-O- (3-[35S]thiotriphosphate) binding without receptor down-regulation in brain ... 5.. Devane WA, Dysarz III FA, Johnson MR, et al. Determination and characterization of a cannabinoid receptor in rat brain. Mol ... Anti-edema and analgesic properties of Δ9-tetrahydrocannabinol (THC). J Pharmacol Exp Ther 1973; 186(3): 646-55PubMedGoogle ... Can J Physiol Pharmacol 2001; 79(11): 942-5PubMedCrossRefGoogle Scholar ...
https://link.springer.com/article/10.2165/00003088-200342040-00003
*  Plus it
1998) Stimulation of guanosine-5′-O-(3-[35S]thio)triphosphate binding by endogenous opioids acting at a cloned mu receptor. J ... thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Mol Pharmacol 47:848-854. ... 1995) Modulation by μ-opioid agonists of guanosine-5′-O-(3-[35S] ... thio]-triphosphate binding. Proc Natl Acad Sci USA 92:7242-7246. ... 3 B), and hypothalamus (Fig. 3 C). Little visible change was ...
http://www.jneurosci.org/content/20/12/4555
*  Frontiers | Receptor-G Protein Interaction Studied by Bioluminescence Resonance Energy Transfer: Lessons from Protease...
Evidence that a beta-adrenergic receptor-associated guanine nucleotide regulatory protein conveys guanosine 5'-O-(3- ... thiotriphosphate) - dependent adenylate cyclase activity. J. Biol. Chem. 256, 8718-8723.. Pubmed Abstract , Pubmed Full Text ... FIGURE 3. Figure 3. GPCR-G protein preassembly or agonist-induced association. (A) In the preassembly model, as shown for PAR1- ... FIGURE 5. Figure 5. The multiple coupling of GPCRs. The diversity of GPCR signaling as a result of the possibility of a given ...
https://www.frontiersin.org/articles/10.3389/fendo.2012.00082/full
*  SMART: RA domain annotation
... complexed to the guanosine triphosphate analogue guanosine-5'-(beta, gamma-imido)triphosphate (GppNp) has been determined at a ... RBD OF RAL GUANOSINE-NUCLEOTIDE EXCHANGE FACTOR (PROTEIN), NMR, 10 STRUCTURES. 3ddc. Crystal Structure of NORE1A in Complex ... RA-DOMAIN OF RAL GUANOSINE-NUCLEOTIDE DISSOCIATION STIMULATOR. 1rlf. STRUCTURE DETERMINATION OF THE RAS-BINDING DOMAIN OF THE ... This protein bound to the GTP gamma S (guanosine 5'-(3-O-thio)triphosphate, a nonhydrolyzable GTP analog).GST-Ha-Ras affinity ...
http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=RA&BLAST=DUMMY
*  Ziprasidone - Wikipedia
Newman-Tancredi A, Audinot V, Chaput C, Verrièle L, Millan MJ (1997). "[35S]Guanosine-5'-O-(3-thio)triphosphate binding as a ... Blockade of 5-HT2A and 5-HT2C and activation of 5-HT1A as well as inhibition of the reuptake of serotonin and norepinephrine ... Blockade of the 5-HT2A receptor may also play a role in its effectiveness against positive symptoms, though the significance of ... 46 (5): 464-94. doi:10.1176/appi.psy.46.5.464. PMID 16145193. Miceli JJ, Anziano RJ, Robarge L, Hansen RA, Laurent A (2000). " ...
https://en.wikipedia.org/wiki/Ziprasidone
*  "Regulation of substance P receptor affinity by guanine nucleotide-bind" by Susan G. MacDonald and Norman D. Boyd
3-O-thio)triphosphate ([35S]GTP gamma S) binding activity. Addition of purified G-proteins from bovine brain to the alkaline- ... Alkalies; Animals; GTP-Binding Proteins; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Triphosphate; Hydrogen-Ion ... guanosine 5'-(3-O-thio)triphosphate ([35S]GTP gamma S) binding activity. Addition of purified G-proteins from bovine brain to ...
https://escholarship.umassmed.edu/gsbs_sp/796/
*  "Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotid" by Jes K. Klarlund, Lucia E. Rameh et al.
Importantly, PtdIns(3,4,5)P3, but not PtdIns(4,5)P2, markedly enhances the ARF exchange activity of GRP1 in a reaction mixture ... PtdIns(3,4,5)P3-mediated ARF nucleotide exchange through GRP1 is selectively blocked by 100 microM inositol 1,3,4,5- ... Further, the Sec7 domain of GRP1 is found to catalyze guanine nucleotide exchange of ARF1 and -5 but not ARF6. ... Taken together, these data are consistent with the hypothesis that selective recruitment of GRP1 to PtdIns(3,4,5)P3 in ...
https://escholarship.umassmed.edu/oapubs/784/
*  SMART: Secondary literature for GAF domain
Cyclic guanosine 5'-monophosphate (cGMP) phosphodiesterase (PDE) regulates the level of cGMP on transduction of a visual signal ... thio)triphosphate did not affect the distance between the probes under conditions where the PDE was activated. These data are ... Binding of the labeled gammaPDE by alphaT.guanosine 5'-3-O-( ... DdPDE3 is inhibited by 3-isobutyl-1-methylxanthine with an IC( ... Neither cGMP nor 3-isobutyl-1-methylxanthine affects Zn2+ binding under the conditions used. Mn2+, Co2+, or Mg2+ supports ...
http://smart.embl.de/smart/show_secondary.cgi?domain=GAF
*  Evidence for the Role of Membrane Receptor Lateral Movement in GTP-Binding Protein-Mediated Signal Transduction | SpringerLink
Subsecond modula tion of formyl peptide-linked guanine nucleotide-binding proteins by guanosine 5'-O-(3-thio)triphosphate in ... Interactions between a lymphoma membrane-associated guanosine 5'-triphosphatebinding protein and the cytoskeleton during ... 5.. Jans DA, Peters R, Jans P et al. Ammonium chloride affects receptor number and lateral mobility of the vasopressin V2-type ... 3.. Jans DA, Peters R, Zsigo J et al. The adenylate cyclase-coupled vasopressin V2-receptor is highly laterally mobile in ...
https://link.springer.com/chapter/10.1007/978-1-4757-0680-2_5