... glutamate dehydrogenase) and other amino acids were taken into account.. As stated before, the predictability is one of the ... For example, G6P concentration was determined in the presence of 5 mM MgSO4 and 0.48 mM NADP+ after addition of 0.7 U ml-1 of ... Glucose-6-phosphate dehydrogenase; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; ICDH: Isocitrate dehydrogenase; HPr: ... Namely, the NADH dehydrogenase NDH-I encoded by nuo gene transports 2 H+/e-, while NDH-II encoded by ndh gene transports 0 H+/e ...

Short-chain dehydrogenase is a very large family of enzymes, most of which are known to be NAD- or NADP-dependent ... CPOAA is formed by first transfer of an N-acetyl group to L-glutamate by PolN to form N-acetyl-L-glutamate, followed by ... To deduce whether polP could cross-complement with its homolog, ArgB (N-acetyl glutamate kinase), the argB gene in S. cacaoi ... For one thing, the hypothetic L-glutamate-γ-semialdehyde in CPOAA biosynthetic pathway was also a confirmed intermediate for L- ...

Isocitrate dehydrogenase (Icdh): 50 mM phosphate buffer (pH 7.5), 5 mM MgCl2, 2 mM NADP+, 2.5 mM D,L isocitrate [81]. ... α-KG is an important metabolite for glutamate biosynthesis (Figure 1). Therefore, a possible flux reduction through Icd is in ... Courtright JB, Henning U: Malate Dehydrogenase Mutants inEscherichia coliK-12. J Bacteriol 1970, 102: 722-728. ... The wavelength and millimolar extinction coefficients for NADH2, NADP+ and NADPH2 were 340 nm and 6.22 cm−1 mM−1, respectively ...

... methylenetetrahydrofolate dehydrogenase (NADP)), MTHFR5 (5,10-methylenetetrahydrofolate reductase (Ferredoxin) Electron ... This gene (CLJU_c37240) was previously annotated as glutamate synthase. Another recent report describes a similar electron- ... Wang S, Huang H, Moll J, Thauer RK: NADP + reduction with reduced ferredoxin and NADP + reduction with NADH are coupled via an ... that couples exergonic reduction of NADP with ferredoxin to drive the endergonic reduction of NADP with NADH had been ...

Enzymatic assay of isocitrate lyase, isocitrate dehydrogenase, and succinate dehydrogenase assay. The enzymatic activity of ... One unit of IDH is the amount of enzyme that will generate one μmol of NADH or NADP per minute at pH of 8.0 and at 37 °C. The ... Nonetheless, other metabolism like amino acids metabolism (alanine, aspartate, and glutamate metabolism), glycerophospholipid ... Meanwhile, the level of isocitrate dehydrogenase was determined using IDH activity assay kit (Sigma-Aldrich, Malaysia) as per ...