Approaching Inner Ear Hair Cell Regeneration Through Non-Viral Gene Delivery  Approaching Inner Ear Hair Cell Regeneration Through Non-Viral Gene Delivery
The current thesis has demonstrated that hWJCs are susceptible to non-viral gene delivery methods, and for the first time non- ... The current thesis has bridged the gap between non-viral gene delivery, stem cell therapy, and tissue engineering, which now ... The work of the current thesis identified and enhanced an effective non-viral gene delivery approach that reprogramed human ... The current thesis examines an opposite approach through an "inside-out" strategy using non-viral gene delivery with ...
https://kuscholarworks.ku.edu/handle/1808/15783
hTERT Extends the Life of Human Fibroblasts without Compromising Type I Interferon Signaling  hTERT Extends the Life of Human Fibroblasts without Compromising Type I Interferon Signaling
Introduction of the viral oncoprotein, simian virus 40 (SV40) large T-antigen, which is frequently used to immortalize cells, ... Treatment with IFN-β resulted in the upregulation of four model IFN stimulated genes (ISGs) in HEL-299 and HEL-TERT cells. Both ... In the current study, we introduced the human TERT (hTERT) gene into a primary human embryonic lung (HEL-299) cell strain, ... Primary cells are often used to study viral replication and host-virus interactions as their antiviral pathways have not been ...
https://kuscholarworks.ku.edu/handle/1808/13285
Cytoplasmic vs. Nuclear RNAi in Caenorhabditis elegans  Cytoplasmic vs. Nuclear RNAi in Caenorhabditis elegans
... such as viral and transposon sequences, and these mechanisms are active in the nucleus as well as the cytoplasm. In ingestion- ... as has previously been observed for mRNA-encoding genes in endogenous chromosomes and transgenes. However, while transgene- ...
https://kuscholarworks.ku.edu/handle/1808/23953
Recently added  Recently added
... gene gain and loss, and changes in gene and protein expression. While studies ... ... Rift Valley Fever is an acute zoonotic viral disease caused by Rift Valley Fever virus (RVFV) that affects ruminants and humans ...
https://kuscholarworks.ku.edu/handle/1808/4471/recent-submissions