Monitoring necrotizing viral retinitis by gene amplification of ocular fluids | IOVS | ARVO Journals  Monitoring necrotizing viral retinitis by gene amplification of ocular fluids | IOVS | ARVO Journals
Monitoring necrotizing viral retinitis by gene amplification of ocular fluids Anne Sikorav; Phuc Le Hoang; Flore Rozenberg; ... Monitoring necrotizing viral retinitis by gene amplification of ocular fluids You will receive an email whenever this article ... No modeling of viral kinetics could be made for HSV2 virus, nor in case of unfavorable evolution with viral load stagnation. In ... Anne Sikorav, Phuc Le Hoang, Flore Rozenberg, Bahram Bodaghi; Monitoring necrotizing viral retinitis by gene amplification of ...
https://iovs.arvojournals.org/article.aspx?articleid=2147656
Protein-based nanoparticles for non-viral retinal gene therapy.  | IOVS | ARVO Journals  Protein-based nanoparticles for non-viral retinal gene therapy. | IOVS | ARVO Journals
Protein-based nanoparticles for non-viral retinal gene therapy. Anna Salas Torras; Laura Fontrodona Montals; Neus Ferrer ... Protein-based nanoparticles for non-viral retinal gene therapy. You will receive an email whenever this article is corrected, ... have been tested on in-vitro and in-vivo models for its application in non-viral retinal gene therapy. ... Protein-based nanoparticles for non-viral retinal gene therapy. . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1537. ...
https://iovs.arvojournals.org/article.aspx?articleid=2331249
Monitoring necrotizing viral retinitis by gene amplification of ocular fluids | IOVS | ARVO Journals  Monitoring necrotizing viral retinitis by gene amplification of ocular fluids | IOVS | ARVO Journals
Monitoring necrotizing viral retinitis by gene amplification of ocular fluids Anne Sikorav; Phuc Le Hoang; Flore Rozenberg; ... Monitoring necrotizing viral retinitis by gene amplification of ocular fluids You will receive an email whenever this article ... No modeling of viral kinetics could be made for HSV2 virus, nor in case of unfavorable evolution with viral load stagnation. In ... Anne Sikorav, Phuc Le Hoang, Flore Rozenberg, Bahram Bodaghi; Monitoring necrotizing viral retinitis by gene amplification of ...
http://iovs.arvojournals.org/article.aspx?articleid=2147656
Effect of CNTF on Retinal Ganglion Cell Survival in Experimental Glaucoma | IOVS | ARVO Journals  Effect of CNTF on Retinal Ganglion Cell Survival in Experimental Glaucoma | IOVS | ARVO Journals
... there was a quantitative increase in CNTF and BDNF expression in retinas exposed to single viral vectors carrying each gene, ... and the helper plasmid pDG that contains both the AAV genes (rep and cap) and the adenovirus genes required for AAV propagation ... Viral titers were determined for each batch of virus by using real-time PCR. The AAV/CNTF was on the order of 4.79 × 1014 ... TrkB gene transfer protects retinal ganglion cells from axotomy-induced death in vivo. J Neurosci. 2002;22:3977-3986. [PubMed] ...
https://iovs.arvojournals.org/article.aspx?articleid=2126596
Conjunctival T-Cell Subpopulations in Sjögrens and Non-Sjögrens Patients with Dry Eye | IOVS | ARVO Journals  Conjunctival T-Cell Subpopulations in Sjögren's and Non-Sjögren's Patients with Dry Eye | IOVS | ARVO Journals
Sjögren's's syndrome: cytokine and Epstein-Barr viral gene expression within the conjunctival epithelium. Invest Ophthalmol ... viral infection, chronic low humidity, wind, or allergy), which repeatedly triggers the lacrimal reflex (the functional unit) ...
https://iovs.arvojournals.org/article.aspx?articleid=2123615
Outflow Tract Ablation Using a Conditionally Cytotoxic Feline Immunodeficiency Viral Vector | IOVS | ARVO Journals  Outflow Tract Ablation Using a Conditionally Cytotoxic Feline Immunodeficiency Viral Vector | IOVS | ARVO Journals
Feline immunodeficiency viral vectors expressing marker genes in other in vivo studies left IOP largely unaffected, and no ... implications for gene therapeutic approaches. Cancer Gene Ther . 2000; 7: 107-117. [CrossRef] [PubMed] ... Stone EM Identification of a gene that causes primary open angle glaucoma. Science . 1997; 275: 668-670. [CrossRef] [PubMed] ... Stromelysin gene transfer into cultured human trabecular cells and rat trabecular meshwork in vivo. Invest Ophthalmol Vis Sci ...
https://iovs.arvojournals.org/article.aspx?articleid=2190183
Desiccating Stress Promotion of Th17 Differentiation by Ocular Surface Tissues through a Dendritic Cell-Mediated Pathway | IOVS...  Desiccating Stress Promotion of Th17 Differentiation by Ocular Surface Tissues through a Dendritic Cell-Mediated Pathway | IOVS...
Jones DT Monroy D Ji Z Atherton SS Pflugfelder SC . Sjögren's syndrome: cytokine and Epstein-Barr viral gene expression within ... IL-17 activates nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. 6,7 The genes encoding IL-17A ... To confirm the gene expression studies, release of Th17-inducing cytokines into the supernatants of 2-day cultured corneal and ... Expression of Th17-associated genes in the cornea, conjunctiva, DCs, and CD4+ T cells was evaluated by real-time PCR. Cytokine ...
https://iovs.arvojournals.org/article.aspx?articleid=2186282
Corneoscleral Limbus in Glaucoma Patients: In Vivo Confocal Microscopy and Immunocytological Study | IOVS | ARVO Journals  Corneoscleral Limbus in Glaucoma Patients: In Vivo Confocal Microscopy and Immunocytological Study | IOVS | ARVO Journals
Sjögren's syndrome: cytokine and Epstein-Barr viral gene expression within the conjunctival epithelium. Invest Ophthalmol Vis ... Antigen-presenting cells are stratified within normal human corneas and are rapidly mobilized during ex vivo viral infection. ...
https://iovs.arvojournals.org/article.aspx?articleid=2277047
Lipid-mediated Gene Transfer of EGFP into Human Corneal Endothelial Cells (HCEC)  | IOVS | ARVO Journals  Lipid-mediated Gene Transfer of EGFP into Human Corneal Endothelial Cells (HCEC) | IOVS | ARVO Journals
Abstract: : Purpose: Although in generally not as efficient as viral vectors, nonviral systems have potential advantages in ... Lipid-mediated gene transfer is a promising approach to target corneal endothelial cells. Careful examination should be ... Efficiency was determined by EGFP-Fluorescence measurement and toxicity by propidium iodide (PI) staining. Results: Gene ... H. Dannowski, J. Bednarz, R. Reszka, K. Engelmann, U. Pleyer; Lipid-mediated Gene Transfer of EGFP into Human Corneal ...
https://iovs.arvojournals.org/article.aspx?articleid=2412924
TLR3, RIG-1 and MDA5 are constitutively expressed on human corneal limbal fibroblasts and induce proinflammatory response |...  TLR3, RIG-1 and MDA5 are constitutively expressed on human corneal limbal fibroblasts and induce proinflammatory response |...
In response to virus infections, viral components are recognized by pattern recognition receptors (PRRs). Upon ligand binding, ... PRRs activate cytokine genes that set into motion a complex inflammatory cascade. We previously reported that human corneal ...
http://iovs.arvojournals.org/article.aspx?articleid=2146737
Current Prospects in Optic Nerve Protection and Regeneration: Sixth ARVO/Pfizer Ophthalmics Research Institute Conference |...  Current Prospects in Optic Nerve Protection and Regeneration: Sixth ARVO/Pfizer Ophthalmics Research Institute Conference |...
The discussion addressed the possibility of using RNAi in preference to viral vectors to target genes that are difficult to ... Depending on the pathway targeted for gene therapy, it would be appropriate to examine cellular reactivity elicited by viral ... Genes that appear upregulated in early glaucomatous damage include immediate response genes such as Atf3, neurotrophic factor, ... Duration of gene silencing after administration of the RNAi should also be evaluated. Compensatory changes in endogenous gene ...
https://iovs.arvojournals.org/article.aspx?articleid=2188599
Corneal Gene Therapy: Magnetofection | IOVS | ARVO Journals  Corneal Gene Therapy: Magnetofection | IOVS | ARVO Journals
Viral-based gene therapy is efficient but restricted by potential inflammatory response and difficulties in restricting the ... This study aims to transfect mouse cornea with a non-viral based magnetofection method for the targeted delivery of non-viral ... Keywords: 538 gene transfer/gene therapy • 607 nanotechnology • 479 cornea: clinical science ... Corneal Gene Therapy: Magnetofection You will receive an email whenever this article is corrected, updated, or cited in the ...
http://iovs.arvojournals.org/article.aspx?articleid=2268754
Induction of Differentiation by Pyruvate and DMEM in the Human Retinal Pigment Epithelium Cell Line ARPE-19 | IOVS | ARVO...  Induction of Differentiation by Pyruvate and DMEM in the Human Retinal Pigment Epithelium Cell Line ARPE-19 | IOVS | ARVO...
Vollrath D Feng W Duncan JL . Correction of the retinal dystrophy phenotype of the RCS rat by viral gene transfer of Mertk. ... Gene. Forward Primer 5′-3′. Reverse Primer 5′-3′. Accession. Size (bp). Temperature 3°C. ... Gene. Forward Primer 5′-3′. Reverse Primer 5′-3′. Accession. Size (bp). Temperature 3°C. ... Sharma RK Orr WE Schmitt AD Johnson DA . A functional profile of gene expression in ARPE-19 cells. BMC Ophthalmol. 2005;5:25. [ ...
https://iovs.arvojournals.org/article.aspx?articleid=2165368
Tau dysfunction in glaucoma: modulation of optic nerve axonal transport via gene therapy | IOVS | ARVO Journals  Tau dysfunction in glaucoma: modulation of optic nerve axonal transport via gene therapy | IOVS | ARVO Journals
Administration of BDNF and TrkB via an RGC-specific viral vector improves ONAT in a tauopathy model. Studies are ongoing to ... Tau dysfunction in glaucoma: modulation of optic nerve axonal transport via gene therapy ... Tau dysfunction in glaucoma: modulation of optic nerve axonal transport via gene therapy ... modulation of optic nerve axonal transport via gene therapy. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3716. ...
https://iovs.arvojournals.org/article.aspx?articleid=2691516
Systemic Omega-6 Essential Fatty Acid Treatment and PGE1 Tear Content in Sjögrens Syndrome Patients | IOVS | ARVO Journals  Systemic Omega-6 Essential Fatty Acid Treatment and PGE1 Tear Content in Sjögren's Syndrome Patients | IOVS | ARVO Journals
Sjögren's syndrome: cytokine and Epstein-Barr viral gene expression within the conjunctival epithelium. Invest Ophthalmol Vis ... Prostaglandin E1 inhibits collagenase gene expression in rabbit synoviocytes and human fibroblasts. Endocrinology. 1992;131:21- ...
https://iovs.arvojournals.org/article.aspx?articleid=2123999
Wild Type and Mutant Retinoschisin Subunits Co-assemble When Expressed in the Same Cells | IOVS | ARVO Journals  Wild Type and Mutant Retinoschisin Subunits Co-assemble When Expressed in the Same Cells | IOVS | ARVO Journals
RS1 gene delivery via viral vectors to RS1 KO mice has resulted in significant preservation of retinal structure and function. ... Purpose : X-linked retinoschisis (XLRS) is caused by mutations in the gene encoding the secreted protein retinoschisin (RS1). ... and secretion assays to investigate the co-assembly of mutant and wild type RS1 to better understand the potential of gene ...
https://iovs.arvojournals.org/article.aspx?articleid=2691007
Inhibition of Corneal Angiogenesis with a Lentiviral-Medicated Expression of a Fusion MIG:IP-10 Gene  | IOVS | ARVO Journals  Inhibition of Corneal Angiogenesis with a Lentiviral-Medicated Expression of a Fusion MIG:IP-10 Gene | IOVS | ARVO Journals
Recombinant viral particles were tested for in vitro expression of the gene by RT-PCR of transduced human microvascular ... CM Barone; Inhibition of Corneal Angiogenesis with a Lentiviral-Medicated Expression of a Fusion MIG:IP-10 Gene . Invest. ... Inhibition of Corneal Angiogenesis with a Lentiviral-Medicated Expression of a Fusion MIG:IP-10 Gene ... Inhibition of Corneal Angiogenesis with a Lentiviral-Medicated Expression of a Fusion MIG:IP-10 Gene ...
https://iovs.arvojournals.org/article.aspx?articleid=2419033
CRISPR/Cas9-mediated genome editing for correction of inherited retinal disease mutations. | IOVS | ARVO Journals  CRISPR/Cas9-mediated genome editing for correction of inherited retinal disease mutations. | IOVS | ARVO Journals
Purpose : Viral-mediated gene therapy is an exciting development towards curing inherited blindness. However, some genes exceed ... Conclusions : Successful gene editing targeting three different classes of Mendelian mutations at several disease-causing loci ... the carrying capacity of viral vectors or require precise regulation of expression that is difficult to achieve using ...
http://iovs.arvojournals.org/article.aspx?articleid=2559851
Suppression of HSP27 Restores Retinal Function and Protects Photoreceptors From Apoptosis in a Light-Induced Retinal...  Suppression of HSP27 Restores Retinal Function and Protects Photoreceptors From Apoptosis in a Light-Induced Retinal...
Subretinal Transplantation of Viral Vector. A lentiviral-silencing sequence for the rat HSP27 (sHSP27) gene (NCBI Reference ... Quantitative analysis of the gene expression of different retinal cell markers using real-time PCR. The gene expression levels ... Quantitative analysis of the gene expression of different retinal cell markers using real-time PCR. The gene expression levels ... Quantitative analysis of the gene expression of different retinal cell markers using real-time PCR. The gene expression levels ...
https://iovs.arvojournals.org/article.aspx?articleid=2634367
HSV-1 Migration in Latently Infected and Naive Rabbits after Penetrating Keratoplasty | IOVS | ARVO Journals  HSV-1 Migration in Latently Infected and Naive Rabbits after Penetrating Keratoplasty | IOVS | ARVO Journals
More viral DNA and viral gene transcripts were found in tissues from rabbits latently infected with 17Pr (LAT+) than in rabbits ... contained viral DNA. Viral DNA and gene transcripts were detected in 2 of 6 TG from naive rabbits that received grafts from ... PCR primer pairs specific for viral and rabbit cellular gene were used either separately or together. When the actin and VP5 ... Locations of the primer sequence for viral genes are based on the sequence of the 17syn+ strain of HSV-1. ...
https://iovs.arvojournals.org/article.aspx?articleid=2199783