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*  Synthetic Gene Networks That Count | Science
Synthetic gene circuits have enlarged the molecular tool set available to bioengineers and molecular biologists (4, 12-24), and ... Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and ... 1C). T7 RNAP is the gene at the first node driving transcription of T3 RNAP, which ultimately drives transcription of GFP. All ... Because the recombinase gene is inverted with respect to the upstream promoter, further expression of recombinase protein ...
http://science.sciencemag.org/content/324/5931/1199?ijkey=1b1833557b9df0fedab79d216c3af8eecfb2961c&keytype2=tf_ipsecsha
*  Table of Contents - November 27, 2015, 350 (6264) | Science
Gene essentiality and synthetic lethality in haploid human cells. By Vincent A. Blomen, Peter Májek, Lucas T. Jae, Johannes W. ... The core genes essential for life in human cells are defined [Also see Reports by Blomen et al. and Wang et al.] ... Identification and characterization of essential genes in the human genome. By Tim Wang, Kıvanç Birsoy, Nicholas W. Hughes, ... A comprehensive screen identifies genes required for normal and cancerous human cells to keep growing. [Also see Perspective by ...
http://science.sciencemag.org/content/350/6264/
*  Table of Contents - February 09, 2018, 359 (6376) | Science
Programming gene and engineered-cell therapies with synthetic biology. By Tasuku Kitada, Breanna DiAndreth, Brian Teague, Ron ... Gene expression findings from study of hundreds of human brains could lead to diagnostic tests, therapies. ... Neuropsychiatric disease patients have both shared and distinct gene expression pattern changes in the brain compared with ...
http://science.sciencemag.org/content/359/6376/
*  Nucleic acid detection with CRISPR-Cas13a/C2c2 | Science
Paper-based synthetic gene networks. Cell 159, 940-954 (2014). doi:10.1016/j.cell.2014.10.004pmid:25417167. ... Toehold switches: De-novo-designed regulators of gene expression. Cell 159, 925-939 (2014). doi:10.1016/j.cell.2014.10.002pmid: ... G) Schematic of how SHERLOCK is used to distinguish bacterial strains with a universal 16S rRNA gene V3 RPA primer set. (H) ... SNPs in the target are highlighted red or blue, and synthetic mismatches in the guide sequence are in red. (B) Highly specific ...
http://science.sciencemag.org/content/356/6336/438.long
*  Drugging RAS: Know the enemy | Science
Gene essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras. Cell 168, 1-14 (2017). ... They identified five synthetic lethal genes. Although RCE1 and ICMT are enzymes involved in promoting RAS membrane association ... RAS genes have the distinct honor of being the first mutated genes identified in human cancer, ushering in the era of ... Another indirect approach for targeting mutant RAS is based on the concept of synthetic lethality (33). Synthetic lethal ...
http://science.sciencemag.org/content/355/6330/1158
*  Following Gene Duplication, Paralog Interference Constrains Transcriptional Circuit Evolution | Science
Designing synthetic regulatory networks capable of self-organizing cell polarization. Cell 151, 320-332 (2012). doi:10.1016/j. ... C) MADS-box repressed ARG genes. Row 1, ARG3; row 2, ARG5,6. (D) MADS-box activated mating genes (α-specific genes). Row 1, ... Panel 2: MADS-box repressed ARG genes; row 1, ARG3; row 2, ARG5,6. Panel 3: MADS-box activated mating genes (α-specific genes ... Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 158, 113-117 (1995). doi:10.1016/0378-1119(95)00144-U pmid: ...
http://science.sciencemag.org/content/342/6154/104?ijkey=1b29d73fd428cd790347119e220a7fcb22f5b02a&keytype2=tf_ipsecsha
*  SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway | Science
Gene essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras. Cell 168, 890-903.e15 ( ... Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. U.S.A. 89, ... Identification and characterization of essential genes in the human genome. Science 350, 1096-1101 (2015). doi:10.1126/science. ... Histone methylation dynamics and gene regulation occur through the sensing of one-carbon metabolism. Cell Metab. 22, 861-873 ( ...
http://science.sciencemag.org/content/358/6364/813?rss%253D1=
*  Cloning Genes Encoding MHC Class II-Restricted Antigens: Mutated CDC27 as a Tumor Antigen | Science
A series of synthetic peptides were pulsed onto 293IMDR4 for 90 min. After three washes, T cells were added and tested for ... TIL1558 recognized 293IMDR1 transfected with the mutated full-length TPI gene but not with the wild-type TPI gene, nor 293IMDR4 ... The vector pTSX was constructed, which allowed the fusion of 1359mel cDNAs to a gene fragment encoding the first 80 amino acids ... Cloning Genes Encoding MHC Class II-Restricted Antigens: Mutated CDC27 as a Tumor Antigen ...
https://science.sciencemag.org/content/284/5418/1351?ijkey=c867e96e197b343e41dc5ecca5c26be32580c8e8&keytype2=tf_ipsecsha
*  Heterocyst Pattern Formation Controlled by a Diffusible Peptide | Science
The patS gene was identified on the conjugal cosmid 8E11 (5), which suppressed heterocyst development (Fig. 2A). A 3.3-kb ... The addition of a synthetic peptide corresponding to the last five amino acids of PatS inhibited heterocyst development. PatS ... The Ω Spr/Smr cassette replaces a 381-bp Eco RV-Sca I fragment containing the entire patS gene. Four independent isolates with ... We were intrigued with the phosphate regulator (phr) genes from Bacillus subtilis(13) and with phrC in particular, which ...
https://science.sciencemag.org/content/282/5390/935?ijkey=4b0515c86959c8aaecb0d126b89c4bcab2fa0601&keytype2=tf_ipsecsha
*  Tuberous Twosome | Science
Li et al. have used a genetic screen in Drosophila to identify genes that interact in a synthetic lethal manner with Rb. They ... The capacity to identify genes that are deadly only within the context of a cancer cell could yield targeted therapeutics that ... To tackle these problems, cancer biologists have turned to synthetic lethality, which refers to the interaction between two ... genes when disrupting the activity of both together causes a cell to die, whereas inactivation of either gene alone has no ...
http://science.sciencemag.org/content/328/5985/1455.2
*  Editors' Choice | Science
Saxena et al., have created a synthetic gene network that can sense and respond to abnormally high thyroid hormone levels. The ...
http://science.sciencemag.org/content/351/6275/twil
*  Programming self-organizing multicellular structures with synthetic cell-cell signaling | Science
... cells with synthetic cell surface ligands and receptors that controlled gene regulatory circuits based on Notch signaling. ... Programming self-organizing multicellular structures with synthetic cell-cell signaling. By Satoshi Toda, Lucas R. Blauch, ... Programming self-organizing multicellular structures with synthetic cell-cell signaling. By Satoshi Toda, Lucas R. Blauch, ... Programming self-organizing multicellular structures with synthetic cell-cell signaling Message Subject. (Your Name) has ...
http://science.sciencemag.org/content/361/6398/156
*  Ribosomopathies: There's strength in numbers | Science
... are consistent with the previously documented synthetic interactions of the PELOTA homolog DOM34 and various RP genes in yeast ... The ribosomal protein genes and Minute loci of Drosophila melanogaster. Genome Biol. 8, R216 (2007). 10.1186/gb-2007-8-10-r216 ... The gene encoding ribosomal protein S19 is mutated in Diamond-Blackfan anaemia. Nat. Genet. 21, 169-175 (1999). 10.1038/5951 ... Global analyses of gene expression have supported this model of translation (65-67). These analyses highlight the fact that as ...
http://science.sciencemag.org/content/358/6363/eaan2755
*  This Week in Science | Science
... resembling synthetic nickel pincer complexes. Genes encoding accessory proteins involved in the synthesis of this cofactor are ... To determine the associations between genes and local environments, Lasky et al. performed a global genetic survey of nearly ... Identification of the enzyme and its gene enables marker-assisted breeding to put the perfume back into beauty. ...
http://science.sciencemag.org/content/349/6243/twis
*  Table of Contents - April 08, 1977, 196 (4286) | Science
Analysis of chicken ribosomal RNA genes and construction of lambda hybrids containing gene fragments ... Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites ... Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis. By MI Lomax, RB Helling, LI Hecker, SD Schwartzbach, WE ... Gene cloning and containment properties of plasmid Col E1 and its derivatives ...
https://science.sciencemag.org/content/196/4286
*  Remote Enhancement | Science
... the combination of which was thought to be sufficient to activate gene expression. By analyzing mutated versions of a synthetic ... Swanson et al. have dissected the 350-bp sparkling (spa) enhancer that controls expression of the dPax2 gene, which specifies ... The temporal and cell type-specific regulation of gene expression relies in part on enhancers, which are noncoding regions of ... Thus, both distance and the order of regulatory regions enable enhancers to fine-tune gene expression, revealing more levels of ...
http://science.sciencemag.org/content/328/5976/287.1
*  Virus Attenuation by Genome-Scale Changes in Codon Pair Bias | Science
PV-Min has a codon pair bias score (CPB score) much lower than that of normal human genes (Fig. 1B). Both PV-Min and PV-Max ... We next tested whether the synthetic viruses were also attenuated in animals. Viruses were administered to CD155 transgenic (tg ... B) Calculated codon pair bias (CPB) score for all 14,795 annotated human genes. Each dot represents the calculated CPB score of ... Finally, these results suggest that synthetic attenuated virus engineering (SAVE) could play a role in creating new vaccines ...
https://science.sciencemag.org/content/320/5884/1784?ijkey=1234250979ff231c91d72eef88108e7df35e2a17&keytype2=tf_ipsecsha
*  This Week in Science | Science
At metabolic genes, it interacts not with DNA but with other transcription factors that regulate metabolic gene expression in a ... Synthetic Ecology. More than the sum of its parts. *Julia Fahrenkamp-Uppenbrink ... Gene Silencing. Keeping quiet one gene at a time. *Stella M. Hurtley ... But what keeps genes within heterochromatin silent? Tchasovnikarova et al. examined the basis for this type of silencing in ...
http://science.sciencemag.org/content/348/6242/twis
*  This Week in Science | Science
... cells with synthetic cell surface ligands and receptors that controlled gene regulatory circuits based on Notch signaling. ... However, Wang et al. show that synthetic aperture radar (SAR) should be added to the arsenal of techniques used to detect and ... Kuroha et al. identified the genes underlying this phenotype, which encode a component of the gibberellin biosynthetic pathway ... The ability to program the manufacture of biological structures may yield new biomaterials or synthetic tissues and organs. ...
http://science.sciencemag.org/content/361/6398/twis.full
*  Challenges in Engineering Microbes for Biofuels Production | Science
... combinatorial searches for promising target genes and other lab-scale experiments should be conducted with synthetic media. In ... New approaches to biology are being shaped by the genomics revolution; unprecedented ability to transfer genes, modulate gene ... or at most a handful of genes. There is now accumulating evidence that no single gene can endow microbes with tolerance to ... The ability to clone, transfer, and control genes from different organisms, including plants, has reached the point at which ...
http://science.sciencemag.org/content/315/5813/801?ijkey=0cb509350d183ebc3b68bc42cdefd822381e60bb&keytype2=tf_ipsecsha
*  Editors' Choice | Science
When rats were treated with a synthetic glucocorticoid, sex-specific differences in gene expression in the rat liver, as ... When genes implicated in inflammatory disorders were specifically analyzed, gene expression was more affected in males, and ... Nearly 2000 more hormone-responsive genes were detected in females than in males. Moreover, about 70 genes showed opposite ... A number of disease-causing mutations in the human ACTA1 gene that encodes skeletal muscle actin affect residues in the most ...
http://science.sciencemag.org/content/330/6005/twil.full
*  Dynamically Reshaping Signaling Networks to Program Cell Fate via Genetic Controllers | Science
Engineering of cell fate through synthetic gene circuits requires methods to precisely implement control around native decision ... Engineering of cell fate through synthetic gene circuits requires methods to precisely implement control around native decision ... A synthetic control module inserted into yeast cells allows control of cell fate in response to an environmental signal. [Also ... A synthetic control module inserted into yeast cells allows control of cell fate in response to an environmental signal. [Also ...
https://science.sciencemag.org/content/341/6152/1235005
*  Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells | Science
... through a synthetic single-guide RNA (sgRNA) (10), which when targeted to coding regions of genes can create frame shift ... 2A) of 18,080 genes in the human genome with an average coverage of 3 to 4 sgRNAs per gene (table S1), and each target site was ... For a subset of genes, we found enrichment of multiple sgRNAs that target each gene after 14 days of PLX treatment (Fig. 3E), ... 2, D and E, and tables S2 and S3). The overlap in highly depleted genes and functional gene categories between the two cell ...
http://science.sciencemag.org/content/343/6166/84?ijkey=dbeca59cfb5137e14cec0d350feb9be3c8456459&keytype2=tf_ipsecsha
*  News this Week | Science
The sequence of the new gene, which also occurs in humans, resembled that of the yeast autophagy gene 6 (ATG6), and the Dallas ... The Synthetic Biology 1.0 meeting held 2 years ago brought these issues to the forefront and helped prompt the Alfred P. Sloan ... began teasing out the genes needed for autophagy in that simple eukaryote. So far, more than 20 yeast autophagy genes have been ... Yet the synthetic biologists in Berkeley only took baby steps toward self-regulation, suggesting but not voting on a pair of ...
http://science.sciencemag.org/content/312/5777/news-summaries
*  An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen | Science
Two genes, GEM1 and PSD1, showed strong correlation to every ERMES gene. Gem1 is a calcium-binding rho-like GTPase inserted in ... A synthetic biology screen to uncover mutants of the ER-mitochondria connection. (A) Rationale of the screen. (Top) In WT cells ... An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen. By Benoît Kornmann, Erin Currie, Sean R. Collins, ... An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen. By Benoît Kornmann, Erin Currie, Sean R. Collins, ...
http://science.sciencemag.org/content/325/5939/477?ijkey=aa0ef75150be68446257e2294324016992c33e7d&keytype2=tf_ipsecsha