Theory of prokaryotic genome evolution | PNAS
genes over an individual with x. genes. Conveniently, selection coefficients associated with gene acquisition and gene deletion ... genes, and the variation in genome size is attributed to stochastic gain and loss of genes (Fig. 2). New genes are assumed to ... Genes can be acquired by either horizontal gene transfer (in the model, assumed to draw genes from an infinite pool) or ... is the total number of genes, k. i. is the probability of gain of a class i. gene, and the selection landscape s. i. is assumed ...
Antibiotic treatment enhances the genome-wide mutation rate of target cells | PNAS
787 BPSs in 674 genes), as well as those within the treated MMR− lines (8,229 BPSs in 3,144 genes). Then, for each gene, we ... Gene enrichment analyses were then done on list of genes with significantly different expression levels from the negative ... we confirm that one point mutation in the candidate resistance gene marR (the top gene in the wild-type candidate list) ... 2001) Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158( ...
Buffering of deoxyribonucleotide pool homeostasis by threonine metabolism | PNAS
RTG genes), threonine biosynthesis (HOM and THR genes), and permease trafficking (LST genes) pathways coordinately buffer dNTP ... Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... aspects of gene interaction profiles reveal how genes organize in a pathway or cellular process (4, 6). Conceptually, such sets ... Synergistic interactions between HU and threonine biosynthesis genes, but not genes that function in the synthesis of other ...
Aerobic bacterial methane synthesis | PNAS
1 A and B). The ability to convert MeA to CH4 is heritable by a single gene (Figs. 3C and 4) and importantly, MeA→CH4 ... S6 B and E). By contrast, the mutants were reverted back to wild-type status when provided with the plp-aat gene (Fig. 3C). ... The 16S rRNA genes were PCR cloned using 27F and 1492R PCR primers (SI Appendix, Table S2). Amplicons were individually cloned ... The selected genes were refactored to match the codon usage of E. coli using an empirically derived codon usage table to remove ...
Facultative protein selenation regulates redox sensitivity, adipose tissue thermogenesis, and obesity | PNAS
In order to control for the gene expression profile of our cells, the lists of all genes with any (nonselenated) peptides ... We scanned gene sequences with SECISearch3 (17) with default parameters, which returned no SECIS hit. We thus ran an extended ... Genomic Features of Genes Encoding Proteins with Facultative Selenation.. We next investigated whether transcripts encoding ... We also used MEME in an analogous fashion to detect peptide motifs enriched in these genes, also with no results. Lastly, ...
Dollo's law and the death and resurrection of genes | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... Dollos law and the death and resurrection of genes. C R Marshall, E C Raff, and R A Raff. *. Institute for Molecular and ... Using empirical data to assess the rate of loss of coding information in genes for proteins with varying degrees of tolerance ... Conversely, the reactivation of long (, 10 million years)-unexpressed genes and dormant developmental pathways is not possible ...
Brain urea increase is an early Huntington's disease pathogenic event observed in a prodromal transgenic sheep model and HD...
CodeSet designs and a full list of gene names for the 24 target genes and 4 reference genes are provided in Table S8. The ... Key Gene Ontology (GO) terms are shown for each gene (www.geneontology.org/). Bar graphs depicting SLC14A1 expression in the DM ... Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... Specific gene expression differences were detected and validated. Reported functions of the altered genes support the existence ...
Social supports and serotonin transporter gene moderate depression in maltreated children | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... There appears to be a gene by environment interaction between experiences of stress and the serotonin transporter gene, with ... Social supports and serotonin transporter gene moderate depression in maltreated children. Joan Kaufman, Bao-Zhu Yang, Heather ... Social supports and serotonin transporter gene moderate depression in maltreated children. Joan Kaufman, Bao-Zhu Yang, Heather ...
Weak warning signals can persist in the absence of gene flow | PNAS
Gene Flow between Populations.. To assess gene flow between populations, we examined thousands of variable sites (SNPs) across ... Weak warning signals can persist in the absence of gene flow. J. P. Lawrence, Bibiana Rojas, Antoine Fouquet, Johanna Mappes, ... Weak warning signals can persist in the absence of gene flow. J. P. Lawrence, Bibiana Rojas, Antoine Fouquet, Johanna Mappes, ... Weak warning signals can persist in the absence of gene flow. View ORCID ProfileJ. P. Lawrence, View ORCID ProfileBibiana Rojas ...
Human skin long noncoding RNA WAKMAR1 regulates wound healing by enhancing keratinocyte migration | PNAS
S9). Gene set enrichment analysis (GSEA) (30) revealed that a set of cell migration-related genes (Gene Ontology accession GO: ... Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... 5A). In line with this, GSEA revealed that the E2F1-regulated genes [gene set E2F1_Q3 from Molecular Signatures Database v6.1 ( ... A) Chr20 gene expression in keratinocytes with WAKMAR1 knockdown. The gene start distance from the WAKMAR1 transcriptional ...
The regulatory utilization of genetic redundancy through responsive backup circuits | PNAS
Duplicate genes and paralogous gene families long have been perceived as genomic sources of genetics robustness (1-5). The ... In fact, tightly coregulated gene duplicates were found more essential for viability than singleton genes. Functional ... We further suggest, for unidirectional RBCs, the distinction between the responsive gene and the controller gene. ... This pair of genes constitutes an RBC in that utrophin was found to be up-regulated in the absence of its homolog dystrophin ( ...
A soluble bone morphogenetic protein type IA receptor increases bone mass and bone strength | PNAS
BMPR1A is expressed in most tissues throughout development and after birth (20, 21). Gene disruption of Bmpr1a results in ... The effect of mBMPR1A-mFc on BMP signaling was determined using a cell-based luciferase gene reporter assay controlled by SMAD1 ... 2004) Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene ... which interact with Smad4 and translocate to the nucleus to regulate target gene expression. BMPR1A (or ALK3) is a type I ...
Phosphorylation and negative regulation of CONSTITUTIVELY PHOTOMORPHOGENIC 1 by PINOID in Arabidopsis | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... The expression levels of target genes were normalized to the PP2A gene. The primers used in these assays are listed in Table S1 ... 1991) cop1: A regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev 5:1172- ... The gene structure of CSU3 is shown. Black blocks indicate exons, and the intron is represented by a black line. The point ...
Interferon stimulation creates chromatin marks and establishes transcriptional memory | PNAS
Some genes with paused Pol II, such as heat shock genes and LPS responsive genes are induced rapidly after stimulation (34⇓-36 ... 2009) Gene loops function to maintain transcriptional memory through interaction with the nuclear pore complex. Genes Dev 23: ... Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... S2 A and B for Gene Ontology (GO) analysis] (28). Of up-regulated ISGs, approximately half (1,057 genes) displayed a typical ...
Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf | PNAS
Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf. Bénédicte Wenden, David L. K. Toner, ... We used luciferase reporter gene imaging to monitor circadian rhythms in leaves of Arabidopsis thaliana plants, achieving ... Leaves grown without environmental cycles for up to 3 wk reproducibly showed spatiotemporal waves of gene expression consistent ... Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf ...
Embryology of a planktonic tunicate reveals traces of sessility | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... 2001) Brachyury (T) gene expression and notochord development in Oikopleura longicauda (Appendicularia, Urochordata). Dev Genes ... 1996) Requirement of the Manx gene for expression of chordate features in a tailless ascidian larva. Science 274:1205-1208. ... dioica like genes in the case of the tailless ascidian larva of Molgula occulta that is evolutionarily derived from a tailed ...
Evolution of a hot genome | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... Short interspersed nuclear elements (SINEs) are abundant in Solanaceae and have a family-specific impact on gene structure and ... Evolution and Diversification of FRUITFULL Genes in Solanaceae. Dinusha C. Maheepala, Christopher A. Emerling, Alex Rajewski, ... Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses ...
Molecular basis of Tank-binding kinase 1 activation by transautophosphorylation | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... E-mail: erind{at}gene.com.. *. Author contributions: X.M., E.H., Q.T.P., and E.C.D. designed research; X.M., E.H., Q.T.P., R.S. ... 2007) Involvement of the ubiquitin-like domain of TBK1/IKK-i kinases in regulation of IFN-inducible genes. EMBO J 26:3451-3462. ... in turn induce NF-κB-dependent gene transcription via phosphorylation of the inhibitory κBα (IκBα) protein. This modification ...
Previous Special Features | PNAS
Gene Networks in Development and Evolution Special Feature. December 23, 2008; 105 (51) [List of Papers]. Movement Ecology ... Gene Regulatory Networks Special Feature. April 5, 2005; 102 (14) [List of Papers]. Long-Range Electron Transfer Special ...
Nonoverlapping roles of PD-1 and FoxP3 in maintaining immune tolerance in a novel autoimmune pancreatitis mouse model | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... Filled boxes, exons; gray box, 3′UTR of Foxp3 gene; I, IRES. (B) Principle of Cre-Lox-mediated tdRFP. (C) The 3- to ∼11-wk-old ... 2013) Genetic polymorphism in FOXP3 gene: Imbalance in regulatory T-cell role and development of human diseases. J Genet 92(1): ... We bred PD-1 KO mice with mice carrying the IRES-GFPcre reporter KI at the 3′ untranslated region of the FoxP3 gene (hereafter ...
Multispecific targeting of glioblastoma with tumor microenvironment-responsive multifunctional engineered NK cells | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Occurrence of pristine aerosol environments on a polluted planet | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Compliant glass-polymer hybrid single ion-conducting electrolytes for lithium batteries | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Melyrid beetles (Choresine): A putative source for the batrachotoxin alkaloids found in poison-dart frogs and toxic passerine...
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
A genomic and historical synthesis of plague in 18th century Eurasia | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ... Finally, we analyzed the virulence gene profiles for all genomes by using the virulence genes listed in Zhou and Yang (31). ... We analyzed the presence and absence of virulence-associated genes in our new genomes using the gene intervals proposed in Zhou ... Metagenomic microbial community profiling using unique clade-specific marker genes. Nat. Methods 9, 811-814 (2012).. ...
Synergistic dual positive feedback loops established by molecular sequestration generate robust bimodal response | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Abrupt increases in Amazonian tree mortality due to drought-fire interactions | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
Prescriptive scientific narratives for communicating usable science | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...
A functional mammalian display screen identifies rare antibodies that stimulate NK cell-mediated cytotoxicity | PNAS
Journal Club: Gene isolated that triggers asexual births in dandelions. Researchers found a gene that could pave the way toward ...