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*  Gene Knockdown Techniques | ISHAR Online
Knockdown of the Drosophila fused in sarcoma (FUS) homologue causes deficient locomotive behavior and shortening of motoneuron ... In the present study, SIRT1 re-expression or knockdown was induced in hepatoma cell lines and liver normal cell lines. ... Mutations in the fused in sarcoma/translated in liposarcoma gene (FUS/TLS, FUS) have been identified in sporadic and familial ... The identification of mutant strains and genes that are responsive to stress and can alter longevity profiles provides new ...
http://isharonline.org/tags/gene-knockdown-techniques
*  Integrated omics-based pathway analyses uncover CYP epoxygenase-associated networks as theranostic targets for metastatic...
Gene knockdown using the shRNA technique. The shRNA clones were purchased from the National RNAi Core Facility Academia Sinica ... genes based on gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Gene sets were ... Gene identity was established according to their official gene symbols.. We used the R package "Pathifier" to perform pathway- ... The gene list enrichment analysis was conducted according to all known human genes or against RNAseq data curated from TCGA- ...
https://rd.springer.com/article/10.1186%2Fs13046-019-1187-y
*  Osteosarcoma | Cancer Genetics Web
Gene Knockdown Techniques. *Apoptosis. *S100A4. *MicroRNAs. *MDM2. *Bone Cancer. *MMP2. *Receptors, Somatomedin ... Mutated Genes and Abnormal Protein Expression (48). Clicking on the Gene or Topic will take you to a separate more detailed ... Gene ontology analysis of LRWD1 co-expressed genes in Ewing tumors revealed association with DNA replication and analysis of ... This new region is approximately 500 kb and contains at least 7 known genes; however, it excludes 2 previous candidate genes: ...
http://www.cancerindex.org/geneweb/X0203.htm
*  Regulated vesicular trafficking of specific PCDH15 and VLGR1 variants in auditory hair cells.
Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show ... Gene Knockdown Techniques. Hair Cells, Auditory / metabolism, ultrastructure*. Immunoprecipitation. Mice. Mice, Neurologic ... Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show delayed maturation ...
http://www.biomedsearch.com/nih/Regulated-Vesicular-Trafficking-Specific-PCDH15/23035094.html
*  Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.
Gene Knockdown Techniques. Gene Silencing. Humans. Inflammation / complications, metabolism*. Macrophages / metabolism. Mice. ... The knockdown animals displayed significantly reduced plasma levels of IL-2 (Fig. 4E) indicating that Alox15b knockdown may ... we used lentiviral shRNA silencing and bone marrow transplantation to knockdown mouse Alox15b gene expression in LDL-receptor- ... Knockdown of Alox15b was confirmed by Q-PCR of mRNA and western blotting of protein from aortic tissue (Fig. 2B and C) and bone ...
http://www.biomedsearch.com/nih/Arachidonate-15-lipoxygenase-type-B/22912809.html
*  Similarity scoring of gene knockdown impact profiles. S | Open-i
Scoring the overall impact of individual gene knockdowns on the prevalence of different phenotypes. We loo ... Figure 5: Similarity scoring of gene knockdown impact profiles. Scoring the overall impact of individual gene knockdowns on the ... Figure 5: Similarity scoring of gene knockdown impact profiles. Scoring the overall impact of individual gene knockdowns on the ... Nodes correspond to gene knockdown events and are colored according to the scoring scale, as indicated (white-dark red, low- ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3368716_1471-2105-13-45-5&req=4
*  mediaTUM - Medien- und Publikationsserver
Gene Knockdown Techniques; Gene Silencing; Genes, Plant; Green Fluorescent Proteins/genetics/metabolism; Hordeum/enzymology/ ... Gene Knockdown Techniques; Gene Silencing; Genes, Plant; Green Fluorescent Proteins/genetics/metabolism; Hordeum/enzymology/ ... Transient-induced gene silencing of barley HvRBK1 supported penetration by the parasitic fungus Blumeria graminis f. sp. hordei ... Transient knockdown of HvRBK1 also influenced the stability of cortical microtubules in barley epidermal cells. Hence, HvRBK1 ...
http://mediatum.ub.tum.de/1379833
*  mediaTUM - Medien- und Publikationsserver
Ascomycota/metabolism/pathogenicity; Gene Knockdown Techniques; GTPase-Activating Proteins/genetics/metabolism; Hordeum/ ... Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal ... Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal ...
http://mediatum.ub.tum.de/1379818
*  Morphological characteristics of PRPF8 mutants and dele | Open-i
Gene Knockdown Techniques. *High-Throughput Nucleotide Sequencing. *Humans. *Molecular Sequence Data. *Real-Time Polymerase ... Knockdown of PRPF8 in K562 and CD34+ primary bone marrow cells increased proliferative capacity. Whole-RNA deep sequencing of ... Knockdown of PRPF8 in K562 and CD34+ primary bone marrow cells increased proliferative capacity. Whole-RNA deep sequencing of ... Mentions: PRPF8 mutations are generally missense and scattered throughout the gene (Figure 1A,D). The high level of ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4214909_nihms588443f2&req=4
*  FSCN1 | Cancer Genetics Web
Gene Knockdown Techniques. Tag cloud generated 15 March, 2017 using data from PubMed, MeSH and CancerIndex ... Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells.. Asian Pac J Cancer ... Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the ... Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located ...
http://www.cancerindex.org/geneweb/FSCN1.htm
*  HKU Scholars Hub: Rho-kinase 2 is frequently overexpressed in hepatocellular carcinoma and involved in tumor invasion
Gene Knockdown Techniques. en_HK. dc.subject.mesh. Golgi Apparatus - physiology. en_HK. ... Functional characterization of DLC1 gene, a novel tumour suppressor gene frequently deleted in liver cancer. - ... Functional characterization of DLC1 gene, a novel tumour suppressor gene frequently deleted in liver cancer ... Conversely, stable knockdown of ROCK2 by short hairpin RNA approach remarkably reduced HCC cell migration and invasion. ...
http://hub.hku.hk/handle/10722/60563
*  Researchers unlock new CRISPR system for targeting RNA | Broad Institute
Currently, the most common technique for performing gene knockdown is small interfering RNA (siRNA). According to the ... complementing the specificity and simplicity of CRISPR-based DNA editing and offering researchers adjustable gene "knockdown" ... said Eugene Koonin, senior author, and leader of the Evolutionary Genomics Group at the NIH's National Center for Biotechnology ... along with co-authors Eugene Koonin and his colleagues at the NIH, and Konstantin Severinov of Rutgers University-New Brunswick ...
https://www.broadinstitute.org/news/8267
*  ZFIN Publication: Filippi et al., 2012
Gene Expression Regulation, Developmental/drug effects. *Gene Knockdown Techniques. *Hypothalamus/cytology. *Hypothalamus/ ... Morpholino-based knockdown reveals that both Arx and Isl1 are strictly required for prethalamic dopaminergic neuron development ...
https://zfin.org/ZDB-PUB-120702-56
*  ZFIN Publication: Hutchinson et al., 2012
Gene Expression Regulation, Developmental. *Gene Knockdown Techniques. *Green Fluorescent Proteins/genetics. *Green Fluorescent ... Here we find that the cey phenotype is due to absence of the gene transducin (beta)-like 3 (tbl3). The tbl3 homolog in yeast ...
http://zfin.org/ZDB-PUB-120605-3
*  John J Laterra - Research Output - Johns Hopkins University
FasL gene knock-down therapy enhances the antiglioma immune response. Jansen, T., Tyler, B., Mankowski, J. L., Recinos, V. R., ... Tumor-specific imaging through progression elevated gene-3 promoter-driven gene expression. Bhang, H. E. C., Gabrielson, K. L. ... Acyl-CoA synthetase VL3 knockdown inhibits human glioma cell proliferation and tumorigenicity. Pei, Z., Sun, P., Huang, P., Lal ... Imaging techniques 2010 Cyr61 mediates hepatocyte growth factor-dependent tumor cell growth, migration, and Akt activation. ...
https://jhu.pure.elsevier.com/en/persons/john-j-laterra/publications/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
RNAi-mediated gene knockdown is an effective technique downregulating target gene expression. This technique is usually used ... Gene Knockdown Techniques, C. elegans, dsRNA interference, gene knockdown, large scale feeding screen ... In the first part of this video, we present two strategies to simultaneously knock down two genes (called double gene knockdown ... This double gene knockdown approach can be used to dissect interrelationships between genes and can be readily applied in ...
https://www.jove.com/visualize/abstract/23844078/rnai-silencing-hahmg-coa-reductase-gene-inhibits-oviposition
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
RNAi-mediated gene knockdown is an effective technique downregulating target gene expression. This technique is usually used ... In the first part of this video, we present two strategies to simultaneously knock down two genes (called double gene knockdown ... double gene knockdown, vitellogenin gene, vg, ultraspiracle gene, usp, vitellogenin protein, Vg, ultraspiracle protein, USP, ... This double gene knockdown approach can be used to dissect interrelationships between genes and can be readily applied in ...
https://www.jove.com/visualize/abstract/24349018/the-good-bad-ugly-agonistic-behaviour-juvenile
*  Developmental Biology - Wikibooks, open books for an open world
Functions of these various proteins in development are often elucidated experimentally using gene knockdown techniques in ... One key class of genes that are differentially regulated by transcription factors in different cell types are genes for cell ... Transcription factors are the key regulators of which genes are expressed in cells. Transcriptional control in the various ...
https://en.wikibooks.org/wiki/Developmental_Biology
*  "CPEB regulation of human cellular senescence, energy metabolism, and p" by David M. Burns and Joel D. Richter
Knockdown cells that have bypassed senescence undergo little telomere erosion. Surprisingly, knockdown of exogenous CPEB that ... In CPEB knockdown cells, p53 mRNA has an abnormally short poly(A) tail and a reduced translational efficiency, resulting in an ... CPEB knockdown cells have fewer mitochondria than wild-type cells and resemble transformed cells by having reduced respiration ... reintroduction of CPEB into the knockdown cells restores a senescence-like phenotype. ...
https://escholarship.umassmed.edu/oapubs/1912/
*  "Reverse genetic screening reveals poor correlation between morpholino-" by Fatma O. Kok, Masahiro Shin et al.
We found that mutations in only a small proportion of genes caused defects in embryogenesis. Moreover, mutants for ten ... Based on these results, we suggest that mutant phenotypes become the standard metric to define gene function in zebrafish, ... The absence of phenotypes in mutant embryos was not likely due to maternal effects or failure to eliminate gene function. ... we applied site-specific nucleases to generate zebrafish lines bearing individual mutations in more than 20 genes. ...
https://escholarship.umassmed.edu/mccb_pubs/17/
*  Kafri, T.<...
Gene therapy knockdown of VEGFR2 in retinal endothelial cells to treat retinopathy. Simmons, A. B., Bretz, C. A., Wang, H., ... Correction to: Gene therapy knockdown of VEGFR2 in retinal endothelial cells to treat retinopathy (Angiogenesis, (2018), 21, 4 ... Toward Personalized Gene Therapy: Characterizing the Host Genetic Control of Lentiviral-Vector-Mediated Hepatic Gene Delivery. ... Lentiviral vector-based gene therapy and the host genetic background. Kafri, T., Pardo Manuel De Villena, F. P. & Ferris, M. T. ...
https://uncch.pure.elsevier.com/en/persons/tal-kafri
*  Research Interests : Hahn Lab at WHOI : Woods Hole Oceanographic Institution (WHOI)
Development of the morpholino gene knockdown technique in Fundulus heteroclitus: a tool for studying molecular mechanisms in an ... Timme-Laragy, A. R., Karchner, S. I., and Hahn, M. E. (2010). Gene knockdown by morpholino-modified oligonucleotides in the ... The AHR as a Chemical Susceptibility Gene.. We have characterized AHRs in marine mammals, birds, and fish and investigated ... These studies have revealed a greater diversity of AHR genes in fishes as compared to mammals. Initially this involved the ...
http://www.whoi.edu/science/B/people/mhahn/hahn.html
*  Children's National Finds Neuroblastoma Tumor Cure in Mice Studies - Healthcanal.com : Healthcanal.com
Gene knockdown refers to a technique in which the expression of one or more of a cell's genes is reduced. The research also ... "The combination of selective gene knock-down in tumor cells and checkpoint blockade produced a novel, potent T-cell triggered ... As Children's researchers examined the impact of the knockdown of ID-2 protein on a tumor, they implanted N2a, a fast growing ... The study revealed that "knockdown'" of a DNA-protein inhibitor, known as ID-2, in aggressive high-risk solid tumors resulted ...
https://www.healthcanal.com/cancers/65702-children-s-national-finds-neuroblastoma-tumor-cure-in-mice-studies.html
*  A small interfering RNA screen of genes involved in DNA repair identifies tumor-specific radiosensitization by POLQ knockdown. ...
... screen of 200 genes involved in DNA damage repair aimed at identifying genes whose knockdown increased tumor radiosensitivity. ... we identified several genes, such as BRCA2, Lig IV, and XRCC5, whose knockdown is known to cause increased cell ... Subsequent investigations showed that POLQ knockdown resulted in radiosensitization of a panel of tumor cell lines from ... Gene Knockdown Techniques, HeLa Cells, Histones, Humans, Infrared Rays, Neoplasms, RNA, Small Interfering, Radiation Tolerance ...
https://www.rdm.ox.ac.uk/publications/118220
*  Genome-wide association and functional follow-up reveals new loci for kidney function
... suggesting a role for these genes in renal function. By providing new insights into genes that regulate renal function, these ... Morpholino knockdown of mpped2 and casp9 in zebrafish embryos revealed podocyte and tubular abnormalities with altered dextran ... Gene Knockdown Techniques , Medical Genetics , genome-wide , Cyclin-Dependent Kinases , Medicinsk genetik , Genome-Wide ... International Consortium to Identify Genes and Interactions Controlling Oral Clef. NIH. , Genome-wide Search for CVD Gene- ...
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::a3a7aa6d5ce4e38c644e6aab7329d85b