Knockdown of the Drosophila fused in sarcoma (FUS) homologue causes deficient locomotive behavior and shortening of motoneuron ... In the present study, SIRT1 re-expression or knockdown was induced in hepatoma cell lines and liver normal cell lines. ... Mutations in the fused in sarcoma/translated in liposarcoma gene (FUS/TLS, FUS) have been identified in sporadic and familial ... The identification of mutant strains and genes that are responsive to stress and can alter longevity profiles provides new ...
Gene expression knockdown techniques. The expression of genes can be reduced by a variety of means, for example by using ... MOs can knock down gene expression by inhibiting mRNA translation, blocking RNA splicing, or inhibiting miRNA activity and ... This technique is being used to screen the effects of human disease genes in Xenopus and the system is sufficiently efficient ... the use of morpholino-antisense oligonucleotides for gene knockdowns in vertebrate embryos, which is now widely used, was first ...
Gene knockdown, a genetic modification technique. .mw-parser-output table.dmbox{clear:both;margin:0.9em 1em;border-top:1px ... Knockdown or knock-down may refer to: *Knockdown, a situation in full-contact combat sports where a fighter is down or ... Knockdown (arcade game), an arcade game released by Namco. *Knockdown (G.I. Joe), a fictional character in the G.I. Joe ... Knock-down kit, a complete kit needed to assemble a vehicle. *Knockdown resistance, genetic resistance to pyrethroid ...
Gene knockdown using the shRNA technique. The shRNA clones were purchased from the National RNAi Core Facility Academia Sinica ... genes based on gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Gene sets were ... Gene identity was established according to their official gene symbols.. We used the R package "Pathifier" to perform pathway- ... The gene list enrichment analysis was conducted according to all known human genes or against RNAseq data curated from TCGA- ...
Gene Knockdown Techniques. Gene Silencing. Humans. Inflammation / complications, metabolism*. Macrophages / metabolism. Mice. ... The knockdown animals displayed significantly reduced plasma levels of IL-2 (Fig. 4E) indicating that Alox15b knockdown may ... we used lentiviral shRNA silencing and bone marrow transplantation to knockdown mouse Alox15b gene expression in LDL-receptor- ... Knockdown of Alox15b was confirmed by Q-PCR of mRNA and western blotting of protein from aortic tissue (Fig. 2B and C) and bone ...
Knockdowns of FgCYC2A were the most dramatic, affecting the position of dorsal petals in relation to lateral petals, thereby ... Additionally, FgANS knockdowns developed larger flowers with wider corolla tube openings. These results provide a method to ... corolla shape of untreated flowers was compared using canonical variate analysis to knockdown phenotypes of CYCLOIDEA2A ( ... quantify the role that specific genes play in the developmental pathway affecting the dorsoventral axis of symmetry in ...
Gene Expression Regulation, Developmental / drug effects * Gene Knockdown Techniques * HEK293 Cells * Humans ... Knockdown of alr through morpholino antisense oligonucleotide (MO) leads to suppression of liver outgrowth while overexpression ... The dose-dependent and partial suppression of alr expression through MO-mediated knockdown allows the identification of its ...
Gene Knockdown Techniques * Gene Silencing* * Gene Targeting * Immunohistochemistry * Learning* * Memory* * Neuronal Plasticity ... Keywords: BCM rule; Cognitive flexibility; Conditional inducible rat knockdown; LTD; LTP; Operant learning; RNA-binding protein ...
Gene Knockdown Techniques. *Apoptosis. *S100A4. *MicroRNAs. *MDM2. *Bone Cancer. *MMP2. *Receptors, Somatomedin ... Mutated Genes and Abnormal Protein Expression (48). Clicking on the Gene or Topic will take you to a separate more detailed ... Gene ontology analysis of LRWD1 co-expressed genes in Ewing tumors revealed association with DNA replication and analysis of ... This new region is approximately 500 kb and contains at least 7 known genes; however, it excludes 2 previous candidate genes: ...
Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show ... Gene Knockdown Techniques. Hair Cells, Auditory / metabolism, ultrastructure*. Immunoprecipitation. Mice. Mice, Neurologic ... Mouse models carrying mutations for the nine Usher-associated genes have splayed stereocilia, and some show delayed maturation ...
RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus, ... genetic techniques include An Overview of Genetics and Disease, Conversion of Fatty Acid Methyl Esters by Saponification ... Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis, Single-plant, Sterile ... RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus. Jessica K. ...
Gene Knockdown Techniques * Genes * Gene Expression * Precision Medicine * Environmental Exposure * Genetic Background ...
Coculture Techniques. 2. 2014. 1346. 0.090. Why? Gene Knockdown Techniques. 1. 2012. 1689. 0.090. Why? ...
5. Differential gene expression. Expression techniques (Northern and „in situ" hybridization, antibody staining, reporter genes ... Gene knockout and knockdown in developmental genetics. Genome-wide approaches, mutant rescue. ... Combinatorial gene regulation. The roles of Hox genes in development.. 3. Signalling crosstalk - interactions between genetic ... 6. RNA-based gene regulation in development (miRNAs in the genomes, prediction and validation). Heterochronic genes. DNA ...
Techniques used to study cells. *Microscopy and Immunostaining *Gene knockdown and Transfection ... DNA microarray screens of gene expression *Computational genomics approaches are used to find patterns in genomic information [ ...
Gene Knockdown Techniques; Gene Silencing; Genes, Plant; Green Fluorescent Proteins/genetics/metabolism; Hordeum/enzymology/ ... Gene Knockdown Techniques; Gene Silencing; Genes, Plant; Green Fluorescent Proteins/genetics/metabolism; Hordeum/enzymology/ ... Transient-induced gene silencing of barley HvRBK1 supported penetration by the parasitic fungus Blumeria graminis f. sp. hordei ... Transient knockdown of HvRBK1 also influenced the stability of cortical microtubules in barley epidermal cells. Hence, HvRBK1 ...
Ascomycota/metabolism/pathogenicity; Gene Knockdown Techniques; GTPase-Activating Proteins/genetics/metabolism; Hordeum/ ... Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal ... Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal ...
fig02: Knock-down of hccs in medaka recapitulates the phenotypic features of MLS syndromeA-E. Bright-field dorsal (A-C) and ... fig02: Knock-down of hccs in medaka recapitulates the phenotypic features of MLS syndromeA-E. Bright-field dorsal (A-C) and ...
Knockdown of endogenous MIR155 inhibits hypoxia-induced autophagy. (A) Inhibition of MIR155 in hypoxia suppresses GFP-LC3 ... Gene Expression Regulation, Neoplastic. *Gene Knockdown Techniques. *Humans. *Molecular Sequence Data. *Neoplasm Proteins/ ... C) Knockdown of endogenous MIR155 inhibits autophagy. HeLa or CNE cells transfected with LNA-NC or LNA-155 were cultured in 1% ... C) Knockdown of endogenous MIR155 inhibits autophagy. HeLa or CNE cells transfected with LNA-NC or LNA-155 were cultured in 1% ...
Additionally, the technique used in this study successfully identified several other genes already known to play a role in ... The effect of gene knockdown on cell survival has not previously been studied for several of these genes. Colony-forming assays ... we describe a siRNA screen of 200 genes involved in DNA damage repair aimed at identifying genes whose knockdown causes ... screen of 200 genes involved in DNA damage repair aimed at identifying genes whose knockdown increased tumor radiosensitivity. ...
Knockdown of p202 gene was performed using RNA interference technique. The specific nucleotide sequences of siRNA-control ( ... This result indicated that the gene encoding p202 is relatively an early response gene in osteoblast differentiation, and the ... p202 knockdown represses the transcription of OCN. Results in Figure 5 indicated that siRNA-p202 may interfere with the ... p202 knockdown or overexpression affects the association of p202/Runx2 with Id2. Runx2 is a critical transcription factor in ...
Transfected with knockdown shRNA targeting Mre11 gene.. Lysates/proteins at 20 µg per lane.. Secondary. HRP-conjugated Goat ... Developed using the ECL technique. Performed under reducing conditions.. Predicted band size : 79 kDa. Observed band size : 75 ... Developed using the ECL technique. Predicted band size : 79 kDa. Western blot of PC12 (brain)whole cell extracts at 30µg per ... with DNA damage response genes.. PLoS One 12:e0179278 (2017). WB ; Human . Read more (PubMed: 28628639) » ...
Gene Knockdown Techniques. Tag cloud generated 15 March, 2017 using data from PubMed, MeSH and CancerIndex ... Network Analyses of Gene Expression following Fascin Knockdown in Esophageal Squamous Cell Carcinoma Cells.. Asian Pac J Cancer ... Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the ... Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkins lymphoma. A pseudogene of this gene is located ...
Mocellin S, Provenzano M (2004) RNA interference: learning gene knock-down from cell physiology. J Transl Med 2:39. https://doi ... 2017). An especially versatile technique in this respect is stable RNA interference (RNAi), in which an artificially ... 2015). For each construct, expression of the inhibited enzyme gene was measured with RT-qPCR from 6 to 8 independent transgenic ... Arimura G, Ozawa R, Shimoda T, Nishioka T, Boland W, Takabayashi J (2000) Herbivory-induced volatiles elicit defence genes in ...
Currently, the most common technique for performing gene knockdown is small interfering RNA (siRNA). According to the ... complementing the specificity and simplicity of CRISPR-based DNA editing and offering researchers adjustable gene "knockdown" ... said Eugene Koonin, senior author, and leader of the Evolutionary Genomics Group at the NIHs National Center for Biotechnology ... along with co-authors Eugene Koonin and his colleagues at the NIH, and Konstantin Severinov of Rutgers University-New Brunswick ...