Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method
The Salmonella enterotoxin (stn) gene exhibits high homology among S. enterica serovars and S. bongori. A set of 6 specific ... The results indicate that the LAMP method, targeting the stn gene, has great potential for detection of Salmonella in food ... primers targeting the stn gene were designed for detection of Salmonella spp. using the loop-mediated isothermal amplification ...https://www.hindawi.com/journals/bmri/2015/356401/abs/
Identification of Primary Initiation Sites for DNA Replication in the Hamster Dihydrofolate Reductase Gene Initiation Zone
Amplification products were resolved by electrophoresis in 1.8% agarose gel (50), and the radioactive band was eluted in 100 μl ... end of the gene. The major site appears to be conserved among species (37). In the DHFR gene region in Chinese hamster ovary ( ... Gene. 1992;122:313-320. [PubMed]. 20. Dubey D D, Zhu J, Carlson D L, Sharma K, Huberman J A. Three ARS elements contribute to ... Therefore, the DHFR gene initiation zone contains at least two (β and β′) and probably three (β, β′, and γ) primary initiation ...http://pubmedcentralcanada.ca/pmcc/articles/PMC108908/
Novel and existing mutations in the tyrosine kinase domain of the epidermal growth factor receptor are predictors of optimal...
a change; a change in a gene.. oncology. (on-call-o-jee) the branch of medicine concerned with the diagnosis and treatment of ... All specimens were examined for somatic mutations by polymerase chain reaction amplification, and all variants were confirmed ... by multiple independent amplifications. Twenty-five patients were treated with cytoreductive surgery with or without ...http://www.mesothelioma-line.com/2008/11/11/novel-and-existing-mutations-in-the-tyrosine-kinase-domain-of-the-epidermal-growth-factor-receptor-are-predictors-of-optimal-resectability-in-malignant-peritoneal-mesothelioma/
A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer | BMC...
Our LAMP method allowed amplification of all targeted 18S-rRNA genes of the reference plasmids with detection limits of 10-100 ... Loop-mediated isothermal amplification (LAMP), which is an innovative nucleic acid amplification (NAA) technique, is regarded ... Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. Knowlesi. J ... If no amplification product was observed, 8 μL of supernatant was also used as template DNA, and we confirmed the final ...https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2718-9/
Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... a process of in vitro enzymatic amplification of specific nucleic acid sequences within the cells where they originate, can be ... Bacteriophage gene 32 protein has been included in PCR mixtures to improve amplification of long targets (Schwarz et al., 1990 ... Hot start nucleic acid amplification. US7955795. 6 Jun 2003. 7 Jun 2011. Qiagen Gmbh. Method of whole genome amplification with ... Naturally occurring SSBs include the bacteriophage T4 gene 32 protein, the filamentous bacteriophage gene 5 protein, the SSB ...http://www.google.es/patents/US5538871
Mechanisms of Acquired Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors and New Therapeutic...
MET increased gene copy number has also been documented in NSCLC and has been studied as negative prognostic factor. It has ... Keywords: EGFR, Tyrosine kinase inhibitors, resistance, mutations, amplifications, MET, VEGFR, NSCLC, Gefitinib, Erlotinib ... MET increased gene copy number has also been documented in NSCLC and has been studied as negative prognostic factor. It has ... Keywords:EGFR, Tyrosine kinase inhibitors, resistance, mutations, amplifications, MET, VEGFR, NSCLC, Gefitinib, Erlotinib ...http://www.eurekaselect.com/73933/article
Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample...
... loop-mediated isothermal amplification;Salmonella spp.;screening;duck; ... by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample - ... by using a loop-mediated isothermal amplification (LAMP) assay targeting the genomic sequence of the invA gene was developed. ... Tomita, N., Mori, Y., Kanda, H., and Notomi, T. (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and ...http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=CSSPBQ_2013_v33n5_655
Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
Universal amplification of fragmented RNA. US20050208515 *. 4 Nov 2004. 22 Sep 2005. Short Jay M. Method of DNA shuffling with ... Chan Eugene Y.. Methods and products for analyzing polymers. US20020119480 *. 6 Dec 2001. 29 Aug 2002. Lawrence Weir. ... Amplification of nucleic acids with electronic detection. US20050059030 *. 12 Dec 2003. 17 Mar 2005. Nanosphere, Inc.. Direct ... Universal amplification of fragmented RNA. US8389219. 19 Nov 2009. 5 Mar 2013. Qiagen Gaithersburg, Inc.. Detection of nucleic ...http://www.google.ca/patents/US5200313
Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay...
A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of ... Edwards, T and Burke, PA and Smalley, HB and Gillies, L and Hobbs, G (2014) Loop-Mediated Isothermal Amplification Test for ... Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay ... Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay ...http://researchonline.ljmu.ac.uk/7560/
Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii...
Gene. *Gene Expression Omnibus (GEO) Database *Gene Expression Omnibus (GEO) Datasets. *Gene Expression Omnibus (GEO) Profiles ... A fast, sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection ... Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii ...https://www.ncbi.nlm.nih.gov/pubmed/23146414
Folia Parasitologica: Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification ...
This LAMP assay was based on the sequence of highly repetitive B1 gene. The detect... ... a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. ... This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of ... Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP). Xin Hu1, Chang ...http://folia.paru.cas.cz/artkey/fol-201201-0004_Urine_sample_used_for_detection_of_Toxoplasma_gondii_infection_by_loop-mediated_isothermal_amplification_LAMP.php
Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from...
This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to ... was used for optimization of LAMP using six primers that target the HVT070 gene sequence of the virus. These primers can ... Aim: This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative ... Notomi, T., Okayama, H., Yonekawa, T., Watana, K., Amino, N. and Hase, T. (2000). Loop-mediated isothermal amplification of DNA ...http://www.veterinaryworld.org/Vol.10/November-2017/17.html
Frontiers | Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection |...
We demonstrate rapid (70-120 minutes) genetic diagnostics methods utilizing loop-mediated isothermal amplification (LAMP) to ... We demonstrate rapid (70-120 minutes) genetic diagnostics methods utilizing loop-mediated isothermal amplification (LAMP) to ... dye following amplification. In total, 239 duplicate samples were collected (31 blood, 122 urine, 73 mucocutaneous wound/swab, ... dye following amplification. In total, 239 duplicate samples were collected (31 blood, 122 urine, 73 mucocutaneous wound/swab, ...https://www.frontiersin.org/articles/10.3389/fmicb.2017.02211/full
Chromosomal amplifications, 3q gain and deletions of 2q33-q37 are the frequent genetic changes in cervical carcinoma | BMC...
Increase in gene dosage by DNA amplification is a common mechanism to achieve over-expression of genes in tumors. Gene ... there were no reports available on the chromosome amplification in relation to prognosis in CC. Gene amplification, in general ... Chromosomal amplification is a frequent phenomenon in CC. We identified 18 high-level chromosomal amplifications at 15 ... identification of a number of chromosomal regions of amplification in the present study suggests that the gene amplification is ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-4-5
Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated...
This method has been used to confirm whether the amplification products are rooted in the target genes. He and Xu (2011) have ... JN684209.1), the metalloprotease gene of V. vulnificus (GenBank accession no. U50548.1), the ompA gene of V. parahaemolyticus ( ... Lanes 2, 4, 6, 8, 10, 12, 14, and 16 were the amplification products, lanes 1, 3, 5, 7, 9, 11, 13, and 15 were negative control ... 2010), a multipex PCR method was developed by using the rpoB gene to make the identificationg of Vibrio harveyi, V. ...https://springerplus.springeropen.com/articles/10.1186/s40064-016-2760-x
Clinical Utility of Serum Human Epidermal Growth Factor Receptor Type 2 Testing as a Marker of Therapeutic Response In Tissue...
HER2 overexpression occurs in 20.0-30.0% of breast cancers, usually as a result of gene amplification [4,5], and is associated ... or HER2 gene amplification using Fluorescence In Situ Hybridization (FISH) . IHC and FISH require invasive enucleation of ... Poor correlation between weak positivity by immunohistochemistry and gene amplification by fluorescence in situ hybridization. ... Akiyama T, Sudo C, Ogawara H, Toyoshima K, Yamamoto T (1986) The product of the human c-erbB-2 gene: a 185-kilodalton ...https://www.clinmedjournals.org/articles/ijccr/international-journal-of-cancer-and-clinical-research-ijccr-4-089.php?jid=ijccr
Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay, Archives of Virology | 10.1007...
"Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay, Archives of Virology" on DeepDyve, ... Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in ... Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products ... Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay. Yang, Jin; Fang, Mei-xin; Li, Jie; ...https://www.deepdyve.com/lp/springer_journal/detection-of-hepatitis-c-virus-by-an-improved-loop-mediated-isothermal-NXy50Kz6Q0
What is Gene Amplification? (with picture)
Gene amplification is a cellular process in which multiple copies of a gene are produced. Most instances of gene amplification ... Gene amplification, also known as gene duplication or chromosomal duplication, is a cellular process in which multiple copies ... associated with the gene. This usually occurs because of a serious genetic flaw in a cell or group of cells. Gene amplification ... Specifically, amplification occurs in a gene that codes for a protein that is able to selectively pump materials out of the ...http://www.wisegeek.com/what-is-gene-amplification.htm
Gene amplification - Wikipedia
Gene amplification may refer to: Polymerase chain reaction, a synthetic gene amplification Gene duplication, a natural form of ...https://en.wikipedia.org/wiki/Gene_amplification
Scientists Uncover Rules for Gene Amplification
... 03.07.2006. Gene amplification plays an important role in causing cancers via ... Gene amplification is the increase in copy number of a particular piece of DNA and is a hallmark of tumor cells. Amplified ... The first step towards achieving this is to discover the rules that govern whether an amplification event is a double minute or ... It's known that regions of chromosomes that are prone to amplification have palindromic sequences of DNA, which are weak places ...https://www.innovations-report.com/html/reports/life-sciences/report-67204.html
Multiple pathways of selected gene amplification during adaptive mutation. - PubMed - NCBI
Multiple pathways of selected gene amplification during adaptive mutation.. Kugelberg E1, Kofoid E, Reams AB, Andersson DI, ... Multiple pathways of selected gene amplification during adaptive mutation. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17319- ... Multiple pathways of selected gene amplification during adaptive mutation. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17319- ... Multiple pathways of selected gene amplification during adaptive mutation. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17319- ...https://www.ncbi.nlm.nih.gov/pubmed/17082307
Gene amplification | definition of gene amplification by Medical dictionary
... gene amplification explanation free. What is gene amplification? Meaning of gene amplification medical term. What does gene ... Looking for online definition of gene amplification in the Medical Dictionary? ... gene amplification. n.. A cellular process characterized by the production of multiple copies of a particular gene or genes to ... gene amplification. a process in which many copies are made of some genes at one time, while other genes are not replicated. ...http://medical-dictionary.thefreedictionary.com/gene+amplification
Whole Gene Amplification from Archived Formalinfixed, Paraffin-embedded Tissues | Sigma-Aldrich
Whole Gene Amplification from Archived Formalinfixed, Paraffin-embedded Tissues. Application Notes. *High yield from limited ... Whole Gene Amplification from Archived Formalinfixed, Paraffin-embedded Tissues. By: Rosemarie Walter,† James Eliason,†* Yezhou ... The average yield was 4 µg with an average amplification of 200-fold amplification (data not shown). ... Figure 2. Amplification plots for different real-time PCR assays. After each PCR cycle fluorescent intensities were normalized ...https://www.sigmaaldrich.com/technical-documents/articles/biology/wga1-general-info.html
Global Gene Amplification Machine Market Growth Prospects Scenario for 2016-2021 | Medgadget
The report systematically categorizes the Gene Amplification Machine market, expounding the definition of Gene Amplification ... Global Gene Amplification Machine Market Growth Prospects Scenario for 2016-2021. May 30th, 2017 Research N Reports Releases ... "Global Gene Amplification Machine Market Research Report 2016" Purchase This Report by calling ResearchnReports.com at +1-888- ... The report gets at the details of the Gene Amplification Machine market and gives an involutely observation of its applications ...https://www.medgadget.com/2017/05/global-gene-amplification-machine-market-growth-prospects-scenario-for-2016-2021.html
Artificial gene amplification reveals an abundance of promiscuous resistance determinants in Escherichia coli | PNAS
... there was a prevalence of prophage genes and genes of unknown function in our list. Prophage genes are usually assumed to be ... Artificial gene amplification reveals an abundance of promiscuous resistance determinants in Escherichia coli. Valerie W. C. ... Two central tenets of the IAD model are: (i) that gene amplification events are common; and (ii) that promiscuous activities ... 2008) Engineering Escherichia coli heat-resistance by synthetic gene amplification. Protein Eng Des Sel 21:121-125. ...http://www.pnas.org/content/108/4/1484