In addition, techniques developed for the production of "chimeric antibodies," such as the splicing of mouse antibody genes to ... Adenoviral vectors have proven especially useful for gene transfer into eukaryotic cells (Graham F L & Prevec L, In Vaccines: ... Genetic modification of a cell may be accomplished using one or more techniques well known in the gene therapy field. Mulligan ... This gene expression data may be useful in diagnosing and monitoring the course of disease in a patient, in determining gene ...

... breeding program to transfer or breed mutant alleles of a nicotine demethylase gene into other tobaccos, as described herein. ... Other techniques such as in situ hybridization, enzyme staining, and immunostaining also can be used to detect the presence or ... Samples with cleaved bands had a mutant ND gene sequence, while samples lacking a cleaved band had a wild type ND gene sequence ... is screened for a mutation in a gene of interest, e.g., a nicotine demethylase gene. For example, the progeny M2 generation of ...

... and transferred to Amersham Hybond N+ membranes using standard alkaline transfer techniques (Sambrook and Russell, 2000). ... Engineered cells are thus cells having a gene or genes introduced through the hand of man. Recombinantly introduced genes will ... evidence for lateral gene exchange," J. Bacteriol., 176:4572-4582, 1994. Marconi, et al., "Variability of osp genes and gene ... Haas and Meyer, "The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion," Cell, 44:107-115 ...

This technique, or variations thereof, may be used to target upregulated genes associated with Alzheimers disease, such as the ... utilize a delivery system comprising monoclonal antibodies that target transferring receptors at the blood-brain barrier and ... The techniques described in the preceding paragraph allowed the identification of the biomarkers in Table 1. An approximately ... One of skill in the art will recognize that the techniques used to measure the activity of a particular biomarker will vary ...

Gene Therapy Vectors. Broadly, gene therapy seeks to transfer new genetic material to the cells of a patient with resulting ... Using encapsulation techniques, Cells can be transplanted into a host without immune rejection, either with or without use of ... Viruses useful as gene transfer vectors include papovavirus, adenovirus, vaccinia virus, adeno-associated virus, herpesvirus, ... In order for a liposome to be an efficient gene transfer vehicle, the following characteristics should be present: (1) ...

... anisotropic wet etching using hot aqueous KOH and dry/plasma etching techniques such as DRIE. For both etching techniques, the ... The method according to claim 61, wherein the filling is performed with an active fluid transfer device. 64. The method ... The substrate with the DNA microarray of deposited spots may then be used for experiments, such as gene expression monitoring ... This plasma technique rapidly pulses the etchant and passivator gasses alternatively over the substrate. This etch can cut a ...

Gene blocking method. May, 2007. Davidson et al.. 20020042364. Modulation of cholesteryl ester transfer protein (CETP) activity ... The foregoing technique for preparation of the peptide antagonists of this invention is more fully described in Ladner, et al. ... Gene Delivery to Organs. October, 2012. Donahue et al.. 20120156207. METHODS AND COMPOSITIONS FOR INHIBITING CD32B EXPRESSING ... In this process, a gene encoding a potential binding domain, is obtained by random mutagenesis of a limited number of ...

A recombinant pShuttle vector specific to genes is constructed by a standard molecular biological technique. ... Thereafter, the mixture was transferred onto ice, and was left standing for one minute or longer. A PCR buffer, MgCl2, dNTP mix ... 8 shows a base sequence of the X56443 gene. The outer shell 9 is formed of porous silicone and is 0.1 to 2 nm in pore size or ... v) Confirmation of Gene Introduction A culture medium to which 2.0% tryptone peptone, 0.5% yeast extract, 0.5% NaCl, and 1.5% ...

Diabodies can be created from the anti-MISIIR scFv genes using, for example, the same techniques as employed to create the anti ... All 12 unique clones were transferred into the pCYN expression vector (also referred to as pUC119mychis), expressed in E. coli ... The diabody gene can be cloned into a vector such as pUC119 Sfi-NotmycHis (Adams, G. P., et al. (1998) British J. Cancer, 77: ... The genes targeted in this manner can have a direct anti-tumor effect, sensitize the tumor to other agents or increase the ...

METHOD FOR EVALUATING DISPERSION OF MATERIAL FOR LIGHT TO HEAT CONVERSION IN THERMAL TRANSFER FILM AND THERMAL TRANSFER FILM ... Permeability may be measured using any known technique such as described in TAPPI specification T460-96 for Gurley, where ... This method also yields a thinner film than can be obtained by conventional calendering techniques. ... processibility in solid form can be readily incorporated into the ePTFE structure in amounts not possible by current techniques ...

... confirming the role of MDR1 gene in drug resistance (Ueda et al., P.N.A.S. USA 84:3004-8, 1987). Since then, 48 human ABC genes ... This technique relies on the interfacial activity of PEG-X block copolymer conjugate, where X is any hydrophobic polymer ( ... Dissolved proteins will be resolved by 7.5% SDS-PAGE and then transferred to PVDF membranes. Immunoblots will be incubated with ... because this approach could provide sustained gene inhibition (Yague et al., Gene Ther. 11:1170-4, 2004). ...

Eugene, Oreg.). The V6-V8 region of the 16S rDNA corresponding to positions 968 to 1401 in Escherichia coli (Brosius, J., et al ... The incidence of Salmonella in litter was determined by using a drag swab technique. All 20 houses were divided into three ... This involves repeatedly and aseptically transferring the culture to a larger and larger volume to serve as the inoculum for ... A total of 1115 E. coli were collected of which, 441 contained two or more virulence genes and, therefore, were considered to ...

For a review of tissue disaggregation techniques, see Freshney, Culture of Animal Cells. A Manual of Basic Technique, 2d Ed., A ... After overnight transfer on to a PVDF membrane, blots were incubated with a blocking buffered (20 mM Tris-Cl (pH 7.4), 500 mM ... The results demonstrate the ability of oxygen to modulating gene expression and imply that oxygen plays an important role in ... The various techniques, methods, and aspects of the invention described above can be implemented in part or in whole using ...

1. Use a polypropylene transfer pipette to harvest the DCs and transfer to a 50 mL conical tube. Wash each well with 2 mL of ... The nanoparticles were synthesized using a previously reported nanoprecipitation technique. Briefly, to prepare the pure PLGA ... It is an enzyme that in humans is encoded by the MMP2 gene. ... 5. Transfer the mouse to the tissue culture hood. Pin the mouse ... 3. Use a sterile polypropylene transfer pipette to gently remove the cells and transfer to a 50 mL conical tube containing an ...

These techniques include, but are not limited to, the hybridoma technique, the human B-cell hybridoma technique, and the EBV- ... 200 μl of such serum was passively transferred to 6 weeks old C57BL/6 mice. These passive serum transfers were repeated every ... Avian vaccines possessing a positive marker gene. October, 2009. Esaki et al. ... 200 μl of such serum was passively transferred to 6 weeks old female SCID mice. These passive serum transfers were repeated ...

AXMI-018, AXMI-020, and AXMI-021, a family of delta-endotoxin genes and methods for their use. September, 2006. Carozzi et al. ... As specific examples, the quenching can be due to molecular collision, energy transfer such as FRET, photoinduced electron ... and includes proteins made using recombinant techniques, i. e. through the expression of a recombinant nucleic acid. ... transfer such as PET, a change in the fluorescence spectrum (color) of the fluorescent group or moiety or any other mechanism ( ...

According to various embodiments, the CCD can be a frame transfer CCD. A frame transfer CCD has a parallel register that can ... An OLED that is deposited on a surface by vapor-deposition technique can be used. An OLED can be deposited on a surface by, for ... Bronstein, Irena et al., "Chemiluminescent and Bioluminescent reporter gene assays", Analytical Biochemistry, vol. 219, 1994, ... By way of example, for a 256×256 element CCD, each time a single imaging area (one row) is transferred to the serial register ...

The fabI gene from the chromosome of resistant isolates may be PCR amplified, then may be sequenced to determine whether ... The solids were washed with ethyl acetate (2×2 v). The damp solid was transferred into a 20-L plastic bucket containing ethyl ... Dosages for the compositions of the present invention may be readily determined by techniques known to those of skill in the ... The filtrate was divided in to two halves and transferred into two 22-L three-necked round-bottomed flasks with a mechanical ...