Oxford Nanopore sequencing: new opportunities for plant genomics?
Keywords: de novo assembly; Basecalling; MinION flow cell; Oxford Nanopore; gene annotation; third-generation sequencing. ...
A high-coverage genome sequence from an archaic Denisovan individual
Gene Flow * Gene Library * Genetic Variation* * Genome, Human / genetics* * Heterozygote* * Humans * Molecular Sequence Data ...
Native Low-Density Lipoproteins Act in Synergy with Lipopolysaccharide to Alter the Balance of Human Monocyte Subsets and Their...
Flow Cytometry * Gene Expression * Healthy Volunteers * Humans * Interleukin-1beta / genetics* * Interleukin-1beta / immunology ... Flow cytometry assays revealed that nLDL significantly decreases the classical monocyte (CM) percentage and increases the non- ...
Retrotransposon expression and incorporation of cloned human and mouse retroelements in human spermatozoa
Flow Cytometry * Gene Expression Regulation * Genes, Reporter * Green Fluorescent Proteins / biosynthesis * Green Fluorescent ...
Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease
Methods: We enumerated invariant natural killer T cells by flow cytometry with the use of CD1d tetramers loaded with alpha- ... Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-lavage cells for evidence of gene expression of ...
Toll-like receptor 3 expressing tumor parenchyma and infiltrating natural killer cells in hepatocellular carcinoma patients
Flow Cytometry * Gene Expression Regulation, Neoplastic * Humans * Immunohistochemistry * Kaplan-Meier Estimate * Killer Cells ...
A novel transcription factor, T-bet, directs Th1 lineage commitment
Flow Cytometry * Gene Expression / immunology * Genes, Reporter * Green Fluorescent Proteins * Indicators and Reagents / ... Remarkably, retroviral gene transduction of T-bet into polarized Th2 and Tc2 primary T cells redirects them into Th1 and Tc1 ... Ectopic expression of T-bet both transactivates the IFNgamma gene and induces endogenous IFNgamma production. ...
Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation
Microarray analysis of primary adipocytes revealed that multiple genes involved in MHCII antigen processing and presentation ... Flow Cytometry * Genes, MHC Class II / immunology* * HEK293 Cells * Humans * Immunohistochemistry * Inflammation / etiology ...
Microsatellite data suggest significant population structure and differentiation within the malaria vector Anopheles darlingi...
An understanding of A. darlingis population structure and contemporary gene flow patterns is necessary if vector populations ... All of the data suggest that the primary division within A. darlingi corresponds to two white gene genotypes between Amazonia ( ... and are in agreement with previously published mitochondrial COI gene sequences interpreted as incipient species. Overall, it ...
Blood Flow to the Spleen is Altered in a Mouse Model of Spinal Muscular Atrophy
Here, we show by doppler ultrasonography decreased splenic blood flow in Smn2B/- mice. Further, AAV9-SMN gene therapy does not ... Here, we show by doppler ultrasonography decreased splenic blood flow in Smn2B/- mice. Further, AAV9-SMN gene therapy does not ... Blood Flow to the Spleen is Altered in a Mouse Model of Spinal Muscular Atrophy J Neuromuscul Dis. 2020;7(3):315-322. doi: ... 4 Bertarelli Foundation Gene Therapy Platform, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1202 ...
VDR FokI polymorphism is associated with a reduced T-helper cell population under vitamin D stimulation in type 1 diabetes...
Th cell subsets were characterized by flow cytometry and gene expression of VDR was measured by Taqman assay. Finally, the VDR ... Aim of the present study was to investigate the possible functional role of the VDR FokI gene polymorphism in Th cells from T1D ... Results: Significant lower VDR gene expression was observed in non-stimulated and 25(OH)D3 stimulated Th cells from T1D ... Furthermore, several genetic variants in the VDR gene including the VDR FokI (rs10735810) polymorphism have been implicated in ...
Gene expression profiling in patients with chronic obstructive pulmonary disease and lung cancer
The results provide evidence that genes involved in tissue remodeling and repair are differentially regulated in the lungs of ... Gene expression was related to forced expiratory flow between 25 and 75% of forced expiratory volume (FEF(25-75%) % predicted) ... Genes involved in extracellular matrix synthesis/degradation and apoptosis were among the up-regulated genes, whereas genes ... Gene expression profiling in patients with chronic obstructive pulmonary disease and lung cancer Am J Respir Crit Care Med. ...
Expression of Free Fatty Acid Receptor 2 by Dendritic Cells Prevents Their Expression of Interleukin 27 and Is Required for...
DCs incubated with the FFAR2 agonist no longer had gene expression patterns associated with activation or IL27 production. ... lamina propria and mesenteric lymph node tissues were analyzed by RNA sequencing and flow cytometry. Intestinal permeability ...
Both MHC and non-MHC genes regulate inflammation and T-cell response after traumatic brain injury
... as shown both by immunolabeling and flow cytometry, than the PVG strain, dwarfing the effect of Ciita. The PVG-RT1(av1) strain ... Both MHC and non-MHC genes regulate inflammation and T-cell response after traumatic brain injury Brain Behav Immun. 2011 Jul; ... In addition, MHC and Vra4 congenic strains on these backgrounds were studied to give information on MHC and non-MHC gene ... Thus, allelic differences in Vra4, harboring the Ciita gene, was found to regulate expression of the invariant chain at the ...
miRNA-205 affects infiltration and metastasis of breast cancer
Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied ... by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. ...
A Role for the RNA Polymerase Gene Specificity Factor σ54 in the Uniform Colony Growth of Uropathogenic Escherichia coli
... factor is to determine the gene specificity of the RNA polymerase (RNAP). In several diverse bacterial species, the σ,sup,54,/ ... colony appearance in UPEC bacteria represents a putative non-canonical function of σ54 in regulating genetic information flow. ... This finding suggests a role for this σ54 in gene regulation that extends beyond its known function as an RNAP gene specificity ... Hence, the genes that rely upon σ54 for their transcription have a wide range of different functions suggesting that the ...
Joyce A[au] - Search Results - PubMed
Biomarkers and Flow Cytometry Validation (Part 3 - Recommendations on 2019 FDA Immunogenicity Guidance, Gene Therapy ... 2019 White Paper on Recent Issues in Bioanalysis: FDA Immunogenicity Guidance, Gene Therapy, Critical Reagents, ... Bioanalytical Challenges, Strategies for Critical Reagent Management, Biomarker Assay Validation, Flow Cytometry Validation & ...
The pleiotropic effects of decanoic acid treatment on mitochondrial function in fibroblasts from patients with complex I...
... gene expression microarray, qPCR and flow cytometry. Treatment with C10 increased citrate synthase activity, a marker of ... Gene expression analysis and qPCR studies suggested that treating cells with C10 supports fatty acid metabolism, through ... increasing ACADVL and CPT1 expression, whilst downregulating genes involved in glucose metabolism (PDK3, PDK4). PCK2, involved ...
Pds5 cooperates with cohesin in maintaining sister chromatid cohesion
Flow Cytometry * Fungal Proteins* * Genes, Reporter * Humans * Macromolecular Substances * Nuclear Proteins / genetics ...
F11 coagulation factor XI [Homo sapiens (human)] - Gene - NCBI
Sensitivity analysis of a reduced model of thrombosis under flow: Roles of Factor IX, Factor XI, and gamma-Fibrin. Title: ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... Genes with a similar H3K4me3 profile Genes with a similar profile of promoter-activating H3K4me3 modifications across several ... Gene Ontology Provided by GOA Function. Evidence Code. Pubs. enables heparin binding IEA Inferred from Electronic Annotation. ...
The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression
Flow cytometry analysis showed a G(1)/S phase … ... on the expression of the BRCA1 and BRCA2 genes in three breast ... Flow cytometry analysis showed a G(1)/S phase cell cycle-arrest after treatment of the cells with 10 microM lycopene for 48 h. ... The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression Eur J Cancer. 2004 Jul; ... These preliminary results on the effects of lycopene on the expression of BRCA1 and BRCA2 oncosuppressor genes in breast cancer ...
SMAD4 Suppresses Colitis-associated Carcinoma Through Inhibition of CCL20/CCR6-mediated Inflammation
Tumors were quantified histologically, and immune cell populations were analyzed by flow cytometry and immunostaining. ... epithelial-specific Smad4 loss with and without germline deletion of the Ccr6 gene were subjected to colitis and followed for ... We previously reported that colon epithelial cell silencing of Smad4 increased epithelial expression of inflammatory genes, ...
MiR-17-5p and MKL-1 modulate stem cell characteristics of gastric cancer cells
Through RT-PCR, Western Blot, flow cytometry, immunofluorescence, sphere formation xenograft tumor assays and drug resistance, ... and miR-17 and MKL-1 were involved as a critical gene in migration and invasion in the EMT pathway. ...
Introgressed chromosome 2 quantitative trait loci restores aldosterone regulation and reduces response to salt in the stroke...
... but renal plasma flow was decreased in the SP.WKYGla2k and SHRSP strains. The SHRSP strain had higher plasma aldosterone in ... plasma aldosterone levels and 3β hydroxysteroid dehydrogenase mRNA gene expression in response to the salt challenge when ... association with greater adrenal CYP11B2 (aldosterone synthase) and 3β hydroxysteroid dehydrogenase mRNA gene expression when ...
Stimulation of B7-H1 in hepatocarcinoma cells by hepatitis B virus X antigen
Flow cytometry and western-blot further demonstrated that B7-H1 protein synthesis was enhanced in HBx(+)-HepG2 cells. Site- ... κB binding site between 128 and 137 bp upstream of B7-H1 gene transcriptional start site is primarily responsible for HBx- ...
Sickle cell disease
Abnormal sickle-shaped erythrocytes disrupt blood flow in small vessels, and this vaso-occlusion leads to distal tissue ... Several new therapeutic options are in development, including gene therapy and gene editing. Recent advances include systematic ... Abnormal sickle-shaped erythrocytes disrupt blood flow in small vessels, and this vaso-occlusion leads to distal tissue ...
NOTCH3 notch receptor 3 [Homo sapiens (human)] - Gene - NCBI
J Cereb Blood Flow Metab, 2023 Jan. PMID 36254369, Free PMC Article ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... GeneRIFs: Gene References Into Functions Whats a GeneRIF? * The cysteine-altering p.R544C variant in the NOTCH3 gene is a ... Gene type. protein coding. RefSeq status. REVIEWED. Organism. Homo sapiens Lineage. Eukaryota; Metazoa; Chordata; Craniata; ...
High-throughput analysis of multispectral images of breast cancer tissue
To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor requ … ... To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor ... Filters such as Beltrami flow-based reaction-diffusion, directional diffusion, etc., were used to pre-process the images ... between the primary tumor and the gene amplification in the cell-nuclei lining the ductal structures in the breast. ...
NOTCH3 notch receptor 3 [Homo sapiens (human)] - Gene - NCBI
J Cereb Blood Flow Metab, 2023 Jan. PMID 36254369, Free PMC Article ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... GeneRIFs: Gene References Into Functions Whats a GeneRIF? * The cysteine-altering p.R544C variant in the NOTCH3 gene is a ... Gene type. protein coding. RefSeq status. REVIEWED. Organism. Homo sapiens Lineage. Eukaryota; Metazoa; Chordata; Craniata; ...
Interleukin-10 contributes to reservoir establishment and persistence in SIV-infected macaques treated with antiretroviral...
7 Flow Cytometry Core, Emory Vaccine Center, Emory University, Atlanta, Georgia, USA. ... 3 Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA. ...