Keywords: de novo assembly; Basecalling; MinION flow cell; Oxford Nanopore; gene annotation; third-generation sequencing. ...
Gene Flow * Gene Library * Genetic Variation* * Genome, Human / genetics* * Heterozygote* * Humans * Molecular Sequence Data ...
Flow Cytometry * Gene Expression * Healthy Volunteers * Humans * Interleukin-1beta / genetics* * Interleukin-1beta / immunology ... Flow cytometry assays revealed that nLDL significantly decreases the classical monocyte (CM) percentage and increases the non- ...
Flow Cytometry * Gene Expression Regulation * Genes, Reporter * Green Fluorescent Proteins / biosynthesis * Green Fluorescent ...
Methods: We enumerated invariant natural killer T cells by flow cytometry with the use of CD1d tetramers loaded with alpha- ... Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-lavage cells for evidence of gene expression of ...
Flow Cytometry * Gene Expression Regulation, Neoplastic * Humans * Immunohistochemistry * Kaplan-Meier Estimate * Killer Cells ...
Flow Cytometry * Gene Expression / immunology * Genes, Reporter * Green Fluorescent Proteins * Indicators and Reagents / ... Remarkably, retroviral gene transduction of T-bet into polarized Th2 and Tc2 primary T cells redirects them into Th1 and Tc1 ... Ectopic expression of T-bet both transactivates the IFNgamma gene and induces endogenous IFNgamma production. ...
Microarray analysis of primary adipocytes revealed that multiple genes involved in MHCII antigen processing and presentation ... Flow Cytometry * Genes, MHC Class II / immunology* * HEK293 Cells * Humans * Immunohistochemistry * Inflammation / etiology ...
An understanding of A. darlingis population structure and contemporary gene flow patterns is necessary if vector populations ... All of the data suggest that the primary division within A. darlingi corresponds to two white gene genotypes between Amazonia ( ... and are in agreement with previously published mitochondrial COI gene sequences interpreted as incipient species. Overall, it ...
Here, we show by doppler ultrasonography decreased splenic blood flow in Smn2B/- mice. Further, AAV9-SMN gene therapy does not ... Here, we show by doppler ultrasonography decreased splenic blood flow in Smn2B/- mice. Further, AAV9-SMN gene therapy does not ... Blood Flow to the Spleen is Altered in a Mouse Model of Spinal Muscular Atrophy J Neuromuscul Dis. 2020;7(3):315-322. doi: ... 4 Bertarelli Foundation Gene Therapy Platform, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1202 ...
Th cell subsets were characterized by flow cytometry and gene expression of VDR was measured by Taqman assay. Finally, the VDR ... Aim of the present study was to investigate the possible functional role of the VDR FokI gene polymorphism in Th cells from T1D ... Results: Significant lower VDR gene expression was observed in non-stimulated and 25(OH)D3 stimulated Th cells from T1D ... Furthermore, several genetic variants in the VDR gene including the VDR FokI (rs10735810) polymorphism have been implicated in ...
The results provide evidence that genes involved in tissue remodeling and repair are differentially regulated in the lungs of ... Gene expression was related to forced expiratory flow between 25 and 75% of forced expiratory volume (FEF(25-75%) % predicted) ... Genes involved in extracellular matrix synthesis/degradation and apoptosis were among the up-regulated genes, whereas genes ... Gene expression profiling in patients with chronic obstructive pulmonary disease and lung cancer Am J Respir Crit Care Med. ...
DCs incubated with the FFAR2 agonist no longer had gene expression patterns associated with activation or IL27 production. ... lamina propria and mesenteric lymph node tissues were analyzed by RNA sequencing and flow cytometry. Intestinal permeability ...
... as shown both by immunolabeling and flow cytometry, than the PVG strain, dwarfing the effect of Ciita. The PVG-RT1(av1) strain ... Both MHC and non-MHC genes regulate inflammation and T-cell response after traumatic brain injury Brain Behav Immun. 2011 Jul; ... In addition, MHC and Vra4 congenic strains on these backgrounds were studied to give information on MHC and non-MHC gene ... Thus, allelic differences in Vra4, harboring the Ciita gene, was found to regulate expression of the invariant chain at the ...
Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied ... by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. ...
... factor is to determine the gene specificity of the RNA polymerase (RNAP). In several diverse bacterial species, the σ,sup,54,/ ... colony appearance in UPEC bacteria represents a putative non-canonical function of σ54 in regulating genetic information flow. ... This finding suggests a role for this σ54 in gene regulation that extends beyond its known function as an RNAP gene specificity ... Hence, the genes that rely upon σ54 for their transcription have a wide range of different functions suggesting that the ...
Biomarkers and Flow Cytometry Validation (Part 3 - Recommendations on 2019 FDA Immunogenicity Guidance, Gene Therapy ... 2019 White Paper on Recent Issues in Bioanalysis: FDA Immunogenicity Guidance, Gene Therapy, Critical Reagents, ... Bioanalytical Challenges, Strategies for Critical Reagent Management, Biomarker Assay Validation, Flow Cytometry Validation & ...
... gene expression microarray, qPCR and flow cytometry. Treatment with C10 increased citrate synthase activity, a marker of ... Gene expression analysis and qPCR studies suggested that treating cells with C10 supports fatty acid metabolism, through ... increasing ACADVL and CPT1 expression, whilst downregulating genes involved in glucose metabolism (PDK3, PDK4). PCK2, involved ...
Flow Cytometry * Fungal Proteins* * Genes, Reporter * Humans * Macromolecular Substances * Nuclear Proteins / genetics ...
Sensitivity analysis of a reduced model of thrombosis under flow: Roles of Factor IX, Factor XI, and gamma-Fibrin. Title: ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... Genes with a similar H3K4me3 profile Genes with a similar profile of promoter-activating H3K4me3 modifications across several ... Gene Ontology Provided by GOA Function. Evidence Code. Pubs. enables heparin binding IEA Inferred from Electronic Annotation. ...
Flow cytometry analysis showed a G(1)/S phase … ... on the expression of the BRCA1 and BRCA2 genes in three breast ... Flow cytometry analysis showed a G(1)/S phase cell cycle-arrest after treatment of the cells with 10 microM lycopene for 48 h. ... The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression Eur J Cancer. 2004 Jul; ... These preliminary results on the effects of lycopene on the expression of BRCA1 and BRCA2 oncosuppressor genes in breast cancer ...
Tumors were quantified histologically, and immune cell populations were analyzed by flow cytometry and immunostaining. ... epithelial-specific Smad4 loss with and without germline deletion of the Ccr6 gene were subjected to colitis and followed for ... We previously reported that colon epithelial cell silencing of Smad4 increased epithelial expression of inflammatory genes, ...
Through RT-PCR, Western Blot, flow cytometry, immunofluorescence, sphere formation xenograft tumor assays and drug resistance, ... and miR-17 and MKL-1 were involved as a critical gene in migration and invasion in the EMT pathway. ...
... but renal plasma flow was decreased in the SP.WKYGla2k and SHRSP strains. The SHRSP strain had higher plasma aldosterone in ... plasma aldosterone levels and 3β hydroxysteroid dehydrogenase mRNA gene expression in response to the salt challenge when ... association with greater adrenal CYP11B2 (aldosterone synthase) and 3β hydroxysteroid dehydrogenase mRNA gene expression when ...
Flow cytometry and western-blot further demonstrated that B7-H1 protein synthesis was enhanced in HBx(+)-HepG2 cells. Site- ... κB binding site between 128 and 137 bp upstream of B7-H1 gene transcriptional start site is primarily responsible for HBx- ...
Abnormal sickle-shaped erythrocytes disrupt blood flow in small vessels, and this vaso-occlusion leads to distal tissue ... Several new therapeutic options are in development, including gene therapy and gene editing. Recent advances include systematic ... Abnormal sickle-shaped erythrocytes disrupt blood flow in small vessels, and this vaso-occlusion leads to distal tissue ...
J Cereb Blood Flow Metab, 2023 Jan. PMID 36254369, Free PMC Article ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... GeneRIFs: Gene References Into Functions Whats a GeneRIF? * The cysteine-altering p.R544C variant in the NOTCH3 gene is a ... Gene type. protein coding. RefSeq status. REVIEWED. Organism. Homo sapiens Lineage. Eukaryota; Metazoa; Chordata; Craniata; ...
To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor requ … ... To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor ... Filters such as Beltrami flow-based reaction-diffusion, directional diffusion, etc., were used to pre-process the images ... between the primary tumor and the gene amplification in the cell-nuclei lining the ductal structures in the breast. ...
J Cereb Blood Flow Metab, 2023 Jan. PMID 36254369, Free PMC Article ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... GeneRIFs: Gene References Into Functions Whats a GeneRIF? * The cysteine-altering p.R544C variant in the NOTCH3 gene is a ... Gene type. protein coding. RefSeq status. REVIEWED. Organism. Homo sapiens Lineage. Eukaryota; Metazoa; Chordata; Craniata; ...
7 Flow Cytometry Core, Emory Vaccine Center, Emory University, Atlanta, Georgia, USA. ... 3 Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA. ...