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*  Gene Targeting by Homologous Recombination in Drosophila | Science
Gene-targeting components. (Aand B) Testing I-SceI efficiency (16). The reporter constructs were transformed via P elements ( ... Gene knockout by targeting with a truncated gene. The donor DNA used for targeting consists of a truncated gene, missing ... gene. The complete gene is ∼4.5 kb in length, and the nonfunctional partial gene is ∼3.5 kb. (C) The construct for yellow ... These two genes were then assembled into the vector pSE280 (Invitrogen). First, the entire β2FRTβ3* gene was put between Not I ...
http://science.sciencemag.org/content/288/5473/2013?ijkey=c0b7f89a4fc01f4a2d0678734509edb2439d4256&keytype2=tf_ipsecsha
*  The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance | Science
Silencing the barley geneSgt1 reveals its role in R gene-triggered,Rar1-dependent disease resistance. SGT1 associates with SKP1 ... The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance ... The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance ... The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance ...
http://science.sciencemag.org/content/295/5562/2073?ijkey=55d01d732247231d206a53b57b32cde732d4f517&keytype2=tf_ipsecsha
*  Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators | Science
Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators ... Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators ... Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators ... Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators ...
https://science.sciencemag.org/content/262/5133/539?ijkey=0b2ee15cd9f1c018ec78cc356acc9374d2280b77&keytype2=tf_ipsecsha
*  Genomic determinants of coral heat tolerance across latitudes | Science
The dendrograms depict the sharing of genes between categories; the fractions correspond to genes with an unadjusted P , 0.05 ... 2C). An analysis of cellular component categories additionally revealed enrichment of nuclear-encoded mitochondrial membrane ... 2 Gene expression associated with larval heat tolerance.. (A) Bar chart for survival odds under heat stress for each larval ... A scan for positively selected genes in the genomes of humans and chimpanzees. PLOS Biol. 3, e170 (2005). doi:10.1371/journal. ...
http://science.sciencemag.org/content/348/6242/1460
*  Editors' Choice | Science
In turn, BMAL1 protein interacts with molecularclock components to increase muscle-specific gene expression in a daily cyclical ... For instance, the transcription factor MYOD1 serves as the muscle master gene regulator. It mediates gene-expression responses ... and thus keep time and regulate gene expression downstream. Core molecular-clock components can also be expressed in a tissue- ... Hodge et al. observed that MYOD1 can also bind to an enhancer within the clock gene Bmal1 to regulate its expression. ...
http://science.sciencemag.org/content/363/6432/twil.full
*  Following Gene Duplication, Paralog Interference Constrains Transcriptional Circuit Evolution | Science
However, many gene products, such as transcriptional regulators, are components within cooperative assemblies; here, we show ... C) MADS-box repressed ARG genes. Row 1, ARG3; row 2, ARG5,6. (D) MADS-box activated mating genes (α-specific genes). Row 1, ... Panel 2: MADS-box repressed ARG genes; row 1, ARG3; row 2, ARG5,6. Panel 3: MADS-box activated mating genes (α-specific genes ... Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 158, 113-117 (1995). doi:10.1016/0378-1119(95)00144-U pmid: ...
http://science.sciencemag.org/content/342/6154/104?ijkey=1b29d73fd428cd790347119e220a7fcb22f5b02a&keytype2=tf_ipsecsha
*  Table of Contents - November 16, 1984, 226 (4676) | Science
Acid Precipitation: Natural Versus Anthropogenic Components. By JAMES N. GALLOWAY, GENE E. LIKENS, MARK E. HAWLEY ... Homologies between signal transducing G proteins and ras gene products. By JB Hurley, MI Simon, DB Teplow, JD Robishaw, AG ... Phosphatidylinositol as a Component of the Ice Nucleating Site of Pseudomonas syringae and Erwinia herbiola ... A major human histone gene cluster on the long arm of chromosome 1 ...
https://science.sciencemag.org/content/226/4676
*  Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus | Science
However, the position of oriT relative to nes was the same for pLW1043 and pSK41/pGO1 (109 bp downstream from the gene for the ... vanR and vanS encode a two-component, inducible regulatory system; the vanH dehydrogenase produces d-lactate from pyruvate; ... The G+C content of the nine individual genes of Tn1546 varies from 29% to 45%, with an overall average of 39%. The very high ... Nine genes are encoded on the Tn1546 transposon: ORF1 and ORF2 are transposase and resolvase enzymes, respectively, required ...
https://science.sciencemag.org/content/302/5650/1569?ijkey=8cf6c6d455d008280ed43417e5aca750bf6c758c&keytype2=tf_ipsecsha
*  Uncovering disease-disease relationships through the incomplete interactome | Science
2 Topological localization and biological similarity of disease genes. (A) The size of the largest connected component S of ... Additional disease and gene annotation data. For the analysis of the similarity between genes and diseases, we use (i) Gene ... Gene-based disease overlap. The overlap between two gene sets A and B is measured by the overlap coefficient C = ,A∩B,/min(,A,, ... The Gene Ontology Consortium. , Gene ontology: Tool for the unification of biology. Nat. Genet. 25, 25-29 (2000). doi:10.1038/ ...
https://science.sciencemag.org/content/347/6224/1257601.long
*  Quinones as the Redox Signal for the Arc Two-Component System of Bacteria | Science
Thus, the Arc signal transduction system provides a link between the electron transport chain and gene expression. ... The Arc two-component signal transduction system mediates adaptive responses of Escherichia coli to changing respiratory ... Quinones as the Redox Signal for the Arc Two-Component System of Bacteria ... Quinones as the Redox Signal for the Arc Two-Component System of Bacteria ...
http://science.sciencemag.org/content/292/5525/2314
*  The Unfolded Protein Response: From Stress Pathway to Homeostatic Regulation | Science
CHOP is a transcription factor that controls genes encoding components involved in apoptosis. Thus, the PERK branch of the UPR ... Two important target genes driven by ATF4 are CHOP (transcription factor C/EBP homologous protein) and GADD34 (growth arrest ... Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2α (eIF2α) dephosphorylation in mammalian ... The altered gene expression resulting from their action in turn affects the cell as a whole. ...
https://science.sciencemag.org/content/334/6059/1081?ijkey=a6f8df516d3b16d973827045f14541a4ae7ad383&keytype2=tf_ipsecsha
*  Self-Recognition in Social Amoebae Is Mediated by Allelic Pairs of Tiger Genes | Science
Kin discrimination depends on strain relatedness, and the polymorphic genes tgrB1 and tgrC1 are potential components of that ... Here, we demonstrate a direct role for these genes in kin discrimination. We show that a matching pair of tgrB1 and tgrC1 ... Self-Recognition in Social Amoebae Is Mediated by Allelic Pairs of Tiger Genes ... Self-Recognition in Social Amoebae Is Mediated by Allelic Pairs of Tiger Genes ...
http://science.sciencemag.org/content/333/6041/467
*  Combinatorial Control Required for the Specificity of Yeast MAPK Signaling | Science
1A). Disruption of the TEC1 gene also reduced FRE activity (Fig. 1A). In contrast, mutations in components of the pheromone ... The expression of the reporter gene FG(TyA)::lacZ depends specifically on the MAPK signaling components that promote ... To test this, we placed the 27-bp Ty1 FRE upstream of an enhancerless CYC1::lacZ reporter gene to create FRE(Ty1):: lacZ (13). ... Because common signaling components such as the MAPK cascade respond to a large number of different stimuli, it is not clear ...
https://science.sciencemag.org/content/275/5304/1314?ijkey=83077181c68329ce21553ee750cc395834484c29&keytype2=tf_ipsecsha
*  A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila | Science
Members of the Argonaute family of proteins are essential components of RNA silencing (4, 5). In Drosophila, five genes encode ... We isolated a full-length cDNA of AGO3, revealing that the AGO3 gene is ∼83 kb in length (fig. S1). Peptide sequence alignments ... M. A. Carmell, Z. Xuan, M. Q. Zhang, G. J. Hannon, Genes Dev. 16, 2733 (2002).. ... K. Miyoshi, H. Tsukumo, T. Nagami, H. Siomi, M. C. Siomi, Genes Dev. 19, 2837 (2005).. ...
https://science.sciencemag.org/content/315/5818/1587?ijkey=3d4925486f0a343288089b048b8a685714b12b93&keytype2=tf_ipsecsha
*  Human telomere biology: A contributory and interactive factor in aging, disease risks, and protection | Science
Hence, other gene networks, in addition to DNA damage and stress responses, are controlled by shelterin components acting as ... Such single-gene inactivating mutations are known in 11 human genes to date. Each mutated gene has a known, molecularly defined ... Mutations in the gene encoding shelterin component POT1 cause a specific human glioma type (brain tumor), and the molecular ... Shelterin components, in turn, are themselves regulated by gene expression and modification pathways that are cell type- and ...
http://science.sciencemag.org/content/350/6265/1193
*  Measurement of gene regulation in individual cells reveals rapid switching between promoter states | Science
... traditional genetic and biochemical approaches usually requires a genetically modified system or assays of purified components ... Cooperativity in long-range gene regulation by the lambda CI repressor. Genes Dev. 18, 344-354 (2004). doi:10.1101/gad.1167904 ... cI gene. (B) Estimating the number of PRM-cI gene copies in lysogenic cells. TetR-YFP binds to an array of tetO sites inserted ... To identify the contribution of a single gene copy, we first examined how the cI gene copy number varies during the cell cycle ...
http://science.sciencemag.org/content/351/6278/1218.full
*  The battle for iron | Science
Host genes important for the immune response to infection often show enhanced rates of diversification, termed positive ... proteins that recognize viruses exhibit amino acid diversity in the specific regions that directly contact components of the ... Bacterial infections are also likely to positively select mammalian genes important for immune defense. On page 1362 of this ...
http://science.sciencemag.org/content/346/6215/1299
*  Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis | Science
Various plant viruses carry repressors of PTGS, combating plant immunity against infection (4, 5). Several components that ... Error bars indicate SD; n = 3. (C) Relative expression levels of select miRNA target genes (ARF and HD-ZIPIII family genes). ... 4E). Thus, biogenesis of ct-siRNAs in many cases could reduce the expression of their cognate genes and compromise gene ... Gene expression and silencing establish the proper transcriptome of eukaryotic cells. Posttranscriptional gene silencing (PTGS ...
https://science.sciencemag.org/content/348/6230/120?ijkey=3fe15da4950343cfdaeed96cdd716efa16e71a8b&keytype2=tf_ipsecsha
*  A Potassium Channel Mutation in Neonatal Human Epilepsy | Science
Although most forms of idiopathic epilepsy have a genetic component, only a few specific syndromes are single-gene disorders (1 ... We obtained partial genomic structure of the KCNQ2 gene by amplifying human genomic DNA with gene-specific primers and ... Gene loci for BFNC have been mapped to chromosome 20q13.3 (3) and to chromosome 8q24 (4). Most families in which the disorder ... By positional cloning, a potassium channel gene (KCNQ2) located on 20q13.3 was isolated and found to be expressed in brain. ...
http://science.sciencemag.org/content/279/5349/403?ijkey=63487adefc847793cfd3aebfae08e1ae7e9c5c1d&keytype2=tf_ipsecsha
*  A Genomic Perspective on Protein Families | Science
Genes from the same species are adjacent; otherwise the gene names are positioned arbitrarily. A unique COG ID is indicated in ... 1C), the symmetrical component of the graph (solid lines) delineates the conserved function of the σ70 subunit of the RNA ... Orthologs are genes in different species that evolved from a common ancestral gene by speciation; by contrast, paralogs are ... A naı̈ve operational definition would simply maintain that for a given gene from one genome, the gene from another genome with ...
https://science.sciencemag.org/content/278/5338/631?ijkey=6de465a83d6f4d012f0a27a086ef8a3265dca9bf&keytype2=tf_ipsecsha
*  A Protein Interaction Map of Drosophila melanogaster | Science
This view shows the fly interaction map with each protein colored by its Gene Ontology Cellular Component annotation. This map ... An additional 34 interactions were identified between protein-coding genes and predicted non-protein-coding genes, 33 with RNA ... These components are connected intimately to the protein ubiquitination machinery via ari-2 and the E2 class of ubiquitin ... 4A, protein disks are color-coded according to broad classes of molecular functions as taken from the Gene Ontology annotations ...
http://science.sciencemag.org/content/302/5651/1727?ijkey=937050dd28e8b64294cd02ebc1f3211267c1c246&keytype2=tf_ipsecsha
*  Editors' Choice | Science
... and it increases the expression of certain genes including the master tumor suppressor gene, p53. The finding by Bochar et al. ... Therefore, the deep Pacific and Indian Oceans that exist today may have been formed with a component of Southern Ocean deep ... Intriguingly, BRG1 binds to BRCA1 within the region encoded by exon 11, the section of the BRCA1 gene that harbors many of the ... Within the SWI/SNF complex, BRCA1 binds to a DNA-dependent ATPase called BRG1, the catalytic component that is essential for ...
http://science.sciencemag.org/content/289/5480/twil
*  Hippo Pathway Inhibits Wnt Signaling to Restrain Cardiomyocyte Proliferation and Heart Size | Science
Embryonic expression of an Nkx2-5/Cre gene using ROSA26 reporter mice. Genesis 31, 176 (2001). doi:10.1002/gene.10022 pmid: ... The mammalian core Hippo signaling components include Ste20 family kinases Mst1 and Mst2, which are homologous to Drosophila ... C) qRT-PCR of Wnt/β-catenin target genes in β-catenin CKO hearts. (D and E) IF images with quantification (F) of E12.5 heart ... Mammals have two Lats genes, Lats1 and Lats2, which are homologous to Drosophila Warts. Lats kinases complex with Mob to ...
https://science.sciencemag.org/content/332/6028/458?ijkey=ca8d3380c25ef97725f2021bc1f94f00382eccf0&keytype2=tf_ipsecsha
*  This Week in Science | Science
... where genes are generally active) and methylation of H3 Lys9 is specific to heterochromatic domains of chromatin (where genes ... Hammond et al. (p. 1146) report the purification of the RISC complex and identify one of its components as the protein ... The two genes, hWNK1 and hWNK4, are expressed in the kidney and encode members of a recently described family of serine- ... Wilson et al. (p. 1107; see the news story by Marx) identify two related genes that are responsible for a rare form of ...
http://science.sciencemag.org/content/293/5532/twis
*  A Stem Cell Molecular Signature | Science
These genes likely represent the conserved molecular components expressed in HSCs. Homologous gene products expressed in the LT ... Hox genes are likely to play a role in HSC regulation. Four HoxA genes are expressed in different subsets of HSCs. Hoxa5 and ... To establish the gene expression profile common for diverse types of SCs, we performed analyses of ESCs and NSCs. Gene products ... ESC/NSC-enriched gene sets were compared with each hematopoietic cluster. These results are summarized in Fig. 3. Gene products ...
https://science.sciencemag.org/content/298/5593/601?ijkey=20902c940e1ed79634c66510486b9925a5474b87&keytype2=tf_ipsecsha