How do you extract DNA from a polyacrylamide gel? I run DGGE gels and need to excise the bands for PCR reamplification and ... DNA extraction from polyacrylamide gels. Vince Mulholland Vince.Mulholland at sasa.gov.uk Thu Aug 24 03:04:23 EST 2000 * ... Do you silver stain your DGGE gels? If you silver stain, do you use a stop solution which contains acetic acid? The acetic acid ...

... Louis Hom lhom at OCF.Berkeley.EDU Wed Feb 11 11:48:57 EST 2004 *Previous message: ... I was also going to suggest gel filtration as an alternative, but then I realized that the problem you may be having is that ... the on-off rates may be causing your complex to get separated to bits in PAGE and probably would have the same problem in gel ...

GEL. Do you know what does LINEAR MODE means? 2) Do you know some laboratory , that are able to use this technique? I am ... In this paper the second dimension of classical 2D-GEL has been sobstitued , with the MALDI technique. My questions are: 1) In ...

Many parallel gel rigs are not a ,,, problem with either. ,,, ,,, DK ,, We use the power pack and it is fine... except that it ...

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The proteins in the middle of the gels disappeared. The bands at the bottom half of the gel and at the very top of the gel ... This occured with more than one gel - all poured at the same time, but electrophoresed separately. Has anyone else encountered ... Hi netters- I recently had some very strange results from my Coumassie stained gels. ...

... absolute protein quantities but only relative quantities for each gel, , by scaling the maximal value on each gel to one, for ...

... pulsed-field gel electrophoresis used for ,epidemiological purposes). I am having problems figuring out how to make ,certain ...

... but straight out of the bottle gels for proteins should work fine for a first approximation. -Dry the gel onto a piece of 3mm ... Dont let your gel get to hot! If you want to run it quickly, then run it in a cold-room. You sometimes have to play around a ... denatured samples on the gel until you get used to the technique-which shouldnt take long. -Eric Cabot - *Previous message: ... Alternates: -Instead of using 32P you can simply silver stain the gel. I originally used silver staining myself, but found that ...

... polyacrylamide gel really carefully, ticking off every step as you do it and :,it not setting? :,Matt My first nasty experience ... APS is usually the biggest problem in my gel pouring experiences. I generally do not use APS which is over a week old (even ... Anything less then 3%T takes a long time to gel. Cheers and good luck ...

I use 10 % acrylamide gel in order to purifyand detect the ~130 kDa , Cry toxin receptor protein by western blot. I used cry4B ...

Amd when the gel was stained with silver I could not see any protein. well there are faint smears here and there but nothing ...

Can also be used to monitor HPLC column regeneration and monitor desalting of proteins by gel filtration. Monitoring of eluent ...

Alternatively does anybody know a recipe for a gel that I could use to detect such a difference? Thanks. Mike Daws *Previous ...

As this program isnt very easy to use, we usually use the Sun-soft- ware only for scanning our gels. For all further work we ... We use a phosphoimager-system named bas1000 from Fuji to scan SDS-PAGE gels with 75-Se-labelled Proteins. Originally it comes ...

So in an IEF gel you could get a group of closely separated bands with the same activity. How does this fit in with isozymes? ...

... am actually searching for a program that would simulate the migration of DNA fragments through an agarose or an acrylamide gel ...

I thought that is a characteristic of the separation of the agarose gels but if this is the case, how can we explain it? ... Hello everyone Does anyone know why in some cases we see (in an 0,8% agarose gel) plasmid DNA higher than chromosomal DNA? I ...

... i could not get any positive result on gel( both sds and native gel). Can anyone please advise me a protocol, at least for ...

I have access to a Mac scanner that gives quite good resolution of sequencing gel negatives and was wondering if anybody knew ...

Getting no ligation whatsoever with different ligases from different companies (BRL, Promega, Boehringer, Pharmacia), gel ...

APS & TEMED in acid gels Matthew Parker *APS & TEMED in acid gels Louis Hom *APS & TEMED in acid gels J C Ford *APS & TEMED in ... APS & TEMED in acid gels The Great American Gene Company *APS & TEMED in acid gels J C Ford *APS & TEMED in acid gels Scott ... protein problems on gels lachlan harris *protein problems on gels Richard Van Frank *protein problems on gels Rod Levine * ... APS & TEMED in acid gels O.C. Meijer *APS & TEMED in acid gels Hideyuki Kajiwara *APS & TEMED in acid gels Dr S. Robertson * ...

Gel Scanning Instrumentation Jonathan A. King *anomalous protein migration on PAGE ? 37_410 at uwovax.uwo.ca *anomalous ...

Protein Elution from SDS Gels Ron Tate *Protein Elution from SDS Gels Federici *Protein Elution from SDS Gels federici *Protein ... Native Protein Gel Protcals/Recipes Needed Ron Tate *Native Protein Gel Protcals/Recipes Needed Uwe Kaerst *Need HGPRTase ... Protein Elution from SDS Gels Tom Deits *Protein Elution from SDS Gels HIRANYA ROYCHOWDHURY * ... detection of dimer in natural gel 312cclai *DF508 CFTR & chaperones L.Kempster *Disulfide bonds in cytoplasmic proteins Stephan ...

running sds gel with guanidium ss at nordicbioscience.com *running sds gel with guanidium Scott Coutts *running sds gel with ...

Protein-Protein Interaction by Native Gel lcrowley at hsc.usf.edu *Protein-Protein Interaction by Native Gel Philipp Pagel * ... Protein-Protein Interaction by Native Gel Louis Hom *Protein-Protein Interaction by Native Gel P.C. *expression periplasm ... Protein-Protein Interaction by Native Gel Greg Pankhurst *Get System Works Pro 2003 by Symantec Norm *Alternatives to Laemli? ...

blotting fixed gels irabinov at bidmc.harvard.edu *blotting fixed gels Hiranya Roychowdhury *blotting fixed gels Peter * ... SDS-PAGE gels at pH 11, pH 12 Ilona Hangyi *Biotin coated on plastic surface Christof Gaenzler *SDS-PAGE gels at pH 11, pH 12 ... Non specific carrier DNA in gel shift assays. Antonin Tutter *Ion Channel Web Page Move J. Christian Hesketh *Owning Your Own ...

2D gel: spot disappearance Jean-Etienne Poirrier *2D: disappearance Anne-Françoise Marchand *2D: disappearance Philipp Wechner ... Acrylamide-Agarose Composite Gels MCCROSKEY, MARK C [R&D/0216] *Assembling databases or multiple databases gene_omic at yahoo. ...

Protein-analysis] Larger Proteins Vanish During Gel Run Steve G *[Protein-analysis] Larger Proteins Vanish During Gel Run ...