Inter Research » DAO » v89 » n1 » p17-27
KEY WORDS: VHSV · Genotyping · Monoclonal antibody · Indirect fluorescent antibody technique · IFAT · ELISA ... One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using ... A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating ... Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa ...http://www.int-res.com/abstracts/dao/v89/n1/p17-27/
Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated...
Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was ... and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA ... Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was ... Inoculated animals had significantly higher N. caninum antibody titers and γIFN production than control animals. One ...http://www.zora.uzh.ch/id/eprint/81986/
Recognition patterns of Neospora caninum tachyzoite antigens by bovine IgG at different IFAT titres | Beef.org.nz
Fluorescent Antibody Technique, Indirect, IFAT, IgG, immunofluorescence test, immunoglobulin G, intermethod comparison, ... This observation was consistent even in the face of fluctuating antibody titre where serum antibody titres from an animal ... Antibody fluctuation was observed across all cows (pregnant and aborted) with no discernible fluctuation pattern. However, the ... Abortion, Veterinary, Animalia, Animals, antibody response, antigen recognition, Antigens, Antigens, Protozoan, article, ...http://beef.org.nz/node/364
Genetics | Topic Collections | JOV | ARVO Journals
TAGS: aspirin, burns, fluorescent antibody technique, indirect, glycogen storage disease type iv, neuregulins, primed in situ ... transfer technique, natural scenes, machine learning Journal of Vision. 2017; 17(3):13. doi: 10.1167/17.3.13 ...http://jov.arvojournals.org/solr/topicresults.aspx?f_Categories=Genetics&resourceid=38481&fd_JournalID=178
Kidney concentrating ability. Medical search. Definitions
... by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of ... FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Sodium-Potassium-Exchanging ... Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, ... It is situated between the PROXIMAL KIDNEY TUBULE and the DISTAL KIDNEY TUBULE.In Situ Hybridization: A technique that ...https://lookformedical.com/definitions.php?q=kidney+concentrating+ability&lang=1
Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection. | Repozitorij Medicinskog...
Western Cell Line Cell Line Tumor Chickenpox/virology Epithelial Cells/virology Fluorescent Antibody Technique,Indirect Herpes ... Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 ... Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus ... We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize ...https://repository.medri.uniri.hr/islandora/object/medri%3A1380
Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research
... and permeabilization technique (Fix and Perm versus Formaldehyde and FACSPerm), of secondary antibody in the indirect labeling ... the DyLight 649 fluorochrome was the preferred choice being characterized by a strong fluorescent signal with low spread and ... and permeabilization technique (Fix and Perm versus Formaldehyde and FACSPerm), of secondary antibody in the indirect labeling ... Indirect staining was performed with the Alexa Fluor 488 labeled antibody because of its low variability and excellent ...https://biblio.ugent.be/publication/1939777
Cryptosporidium - The Full Wiki
This can be done with direct fluorescent antibody (DFA). It can also be achieved through indirect immunofluorescence assay.[ ... Other staining techniques include acid-fast staining, which will stain the oocysts red. One type of acid-fast stain is ... They include microscopy, staining, and detection of antibodies. Microscopy  can help identify oocysts in fecal matter. ... There are several techniques to concentrate either the stool sample or the oocysts. The modified formalin-ethyl acetate (FEA) ...http://www.thefullwiki.org/Cryptosporidium
Entamoeba histolytica Schaudinn ATCC ® 30015™
Comparison of indirect fluorescent-antibody amoebic serology with counterimmunoelectrophoresis and indirect hemagglutination ... Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J. Parasitol. 54: ... monoclonal antibody-based enzyme-linked immunosorbent assay. J. Clin. Microbiol. 32: 964-970, 1994. PubMed: 8027351 ...https://www.atcc.org/en/Products/Quality_Control_Strains/By_Instrument/30015.aspx?p=1&rel=%7B0%7D
Parasitology Centre Europe | Parasite testing, diagnosis and treatment for Europe and the UK.
Anti-echinococcus antibodies can be detected with serodiagnostic tests e.g. Indirect Fluorescent Antibody (IFA) Test, CF ( ... For increased sensitivity, concentration techniques can be used. These include centrifugation of the blood sample lyzed in 2% ... Irrelevant antibodies produced by the host may block the activity of potentially protective antibodies, as has been shown to be ... direct fluorescent antibody [DFA] , and/or enzyme immunoassays for detection of Cryptosporidium sp. antigens). ...https://www.parasitetesting.co.uk/wiki/dog-and-cat-parasites
nanoHUB.org - Resources: [Illinois] ECE 416 Assay Formats
Label-Free Assay method is used with most biosensor surfaces and allows a transducer to detect the presence of the antibodies. ... In this lecture, assays, which is the most widely used technique to detect molecules on surfaces are discussed. In the direct ... the antibodies with enzymes attached and indirect ELISA which contains a secondary Antibody connected to the primary Antibody. ... Many of our techniques are geared for compatibility with flexible plastic materials, leading to applications such as low cost ...http://nanohub.org/resources/16944
Interactions between antigen and antibody - Wiki
Interaction between antigen and antibody is a bimolecular association and it does not lead to an irreversible chemical ... When viewed under fluorescent microscope, the field is dark and areas with bound antibody fluoresce green.. This technique can ... Indirect ELISA. Indirect ELISA helps detecting antibody. It is the method of choice to detect the presence of serum antibodies ... Indirect method. In indirect method, the antibody is not pre-immobilized onto an insoluble support rather, the antibody that is ...http://www.discoverbiotech.com/wiki/-/wiki/Main/Interactions+between+antigen+and+antibody
Immunodiagnosis (complete) Flashcards by Eooooo m | Brainscape
Indirect:. Known bacteria on slide, add pt's serum, wash and added fluorescent-labelled anti-human Ig --- a test for ANTIBODY ... What is the difference between direct and indirect immunofluorescence techniques? Direct: pt swab smeared on slide, fluorescent ...https://www.brainscape.com/flashcards/immunodiagnosis-complete-3465284/packs/5331978
Patent US6149866 - Stopper having a cavity for reagents and an assay method using said stopper - Google Patents
... the antibody or antigen molecules can provide the required signal for the assay. Optical techniques are used to detect reagent ... indirect or inhibitory by their nature. In immunological assays, the antibody binds to an antigen structure which is specific ... fluorescent and phosphorescent molecules and luminescent molecules. ... Prior to the assay, either the antibody or alternatively the antigen may be bound to a specific labelling indicator (marker). ...http://www.google.com/patents/US6149866?dq=7,328,163
Ver por autor "Voller, A"
The fluorescent antibody technique as a measure of antibody to malaria parasites / by A. Voller and R. S. Bray Voller, A; ... Application of the indirect haemagglutination test for malaria / by A. Voller, J. H. E. Th. Meuwissen, T. Goosen Voller, A; ... Fluorescent antibody studies on malaria parasites / by A. Voller Voller, A; World Health Organization (WHO/Mal/344, 1962) ... Application of the indirect haemagglutination test for malaria / by J. H. E. Th. Meuwissen ... [et al.] Meuwissen, J. H. E ...https://apps.who.int/iris/browse?locale-attribute=pt&value=Voller,%20A&type=author
"Developing tTA transgenic rats for inducible and reversible gene expre" by Hongxia Zhou, Cao Huang et al.
To develop transgenic lines for conditional expression of desired genes in rats, we generated several lines of the transgenic rats carrying the tetracycline-controlled transactivator (tTA) gene. Using a vigorous, ubiquitous promoter to drive the tTA transgene, we obtained widespread expression of tTA in various tissues. Expression of tTA was sufficient to strongly activate its reporter gene, but was below the toxicity threshold. We examined the dynamics of Doxycycline (Dox)-regulated gene expression in transgenic rats. In the two transmittable lines, tTA-mediated activation of the reporter gene was fully subject to regulation by Dox. Dox dose-dependently suppressed tTA-activated gene expression. The washout time for the effects of Dox was dose-dependent. We tested a complex regime of Dox administration to determine the optimal effectiveness and washout duration. Dox was administered at a high dose (500 microg/ml in drinking water) for two days to reach the effective concentration, and then was given athttps://jdc.jefferson.edu/pacbfp/119/
METHODS AND COMPOSITIONS FOR THE GENERATION AND USE OF CONFORMATION-SPECIFIC ANTIBODIES - Patent application
... fluorescent marker whose presence and location in a subject can be detected by standard imaging techniques.  The ... detectable substance to the antibody, as well as indirect labeling the antibody by reacting the antibody with another reagent ... antibodies, humanized antibodies, chimeric antibodies, recombinant antibodies, multispecific antibodies, and antibody fragments ... 0074] Alternatively, antibody libraries (e.g., naive antibody libraries, synthetic antibody libraries, semi-synthetic antibody ...http://www.patentsencyclopedia.com/app/20130028900
Fasmatech - MS and Diagnostics
By nature, immunoassays are indirect measurements, i.e. antibody manufacturing is off-line compared to assay deployment. The ... usually using radioactivity or fluorescent techniques. These assays are very sensitive, they can be used for quantitation, they ... Antibody manufacturing (particularly for monoclonal antibodies) is a complicated and very high skill job; it is prone to errors ... Antibody design is time consuming and expensive. It may take weeks or months to develop and fully prepare a new immuno-assay, ...http://fasmatech.com/ms-and-diagnostics/
SIGMA-NOT - PORTAL INFORMACJI TECHNICZNEJ - Najwi ksza baza artyku w technicznych online
In this case, fluorescent dye is used as a label conjugated with the cell/particle markers e.g. antibodies. The simplified ... Different technologies and techniques such as: deep silicon plasma etching in Bosch method, plasma polymerization, soft ... It allows indirect electrochemical measurement of concentration of electrochemically inactive analytes, important in medical ... The detection system was tested for fluorescent dyes - sodium fluoresceinate and resorufin excitated in two systems: static and ...http://sigma-not.pl/wyszukaj-0-0-10-58864252--jerzy-kruk-.html
A guide to integrating immunohistochemistry and chemical imaging - Chemical Society Reviews (RSC Publishing) DOI:10.1039...
The use of antibody labelling with a chemical marker was first described with fluorescent β-anthryl-carbamide conjugated to ... which uses the same parameters to quantify recognition of the primary antibody by indirect detection:. Ψ = n2θ2λ2. (2). ... requiring substantial signal amplification for most detection techniques. Polyclonal antibodies recognise several epitopes on ... Fluorescent proteins. • Live cell imaging. • Resolution and multiplexing limited for large antigen-1°-2°-antibody complexes. ...https://pubs.rsc.org/en/content/articlehtml/2018/cs/c7cs00610a
UMMS scientists reveal new phase of HIV infection
Researchers at the UMass Medical School have identified a new life cycle stage in HIV infection thanks to a novel technique ... This allowed the capsid, tagged with a fluorescent antibody, to be seen in the images of the nucleus.. This technique allows ... Because of this limitation, most insights into HIV's transit across the nuclear membrane have been gained through indirect ... "With our technique we can better determine how HIV establishes itself into our DNA and develop new ways to stop that from ...https://www.brightsurf.com/news/article/121615387923/umms-scientists-reveal-new-phase-of-hiv-infection.html
WO2013142901 SILK POLYPEPTIDES
Techniques for producing and processing polyclonal antisera are known in the art. In order that such antibodies may be made, ... which permit indirect measurement of binding include enzymes where the substrate may provide for a coloured or fluorescent ... If serum containing polyclonal antibodies contains antibodies to other antigens, the polyclonal antibodies can be purified by ... Immortal antibody-producing cell lines can be created by cell fusion, and also by other techniques such as direct ...https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2013142901&recNum=1&maxRec=1000&office=&prevFilter=&sortOption=&queryString=&tab=PCTDescription
Office of Medical History
There was little U.S. experience with this combination of techniques. The SAFA (soluble antigen fluorescent antibody) test ... Vietnam conflict, the Moan precipitin test became obsolete and was replaced by several new techniques. The IHA (indirect ... filarial antibodies as determined by the soluble antigen fluorescent antibody test (Colwell et al. 1970). Microfilaremia, ... Antibody response to invasive amebiasis in Durban, South Africa. Am. J. Trop. Med. 20: 414-20. ...https://history.amedd.army.mil/booksdocs/vietnam/GenMedVN/ch17.html
Immunocytochemistry: Definition, Uses & Techniques | Study.com
Immunocytochemistry is used as a laboratory technique to identify proteins and peptides. This lesson will discuss the ... Immunocytochemistry is done using a direct or indirect method. The direct method uses one antibody while the indirect method ... The antibody is associated with a reporter such as an enzyme, fluorescent dye, or fluorophore. The reporter changes the color ... The primary antibody is applied to the sample and then a secondary reporter-paired antibody is applied to the primary antibody ...https://study.com/academy/lesson/immunocytochemistry-definition-uses-techniques.html
S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt | Virology Journal | Full...
... tracheal and renal histopathology as well as IBV antigens detection by immunofluorescent antibody technique in kidney sections ... Indirect immunofluorescent antibody technique (IFA). It was performed according to  to detect viral antigens in the kidneys ... Slides were then mounted using glycerol/PBS (without allowing the slides to dry) and examined under fluorescent microscopy. ... and used for detection of IBV antigens in both Dot-ELISA and indirect immunofluorescent antibody technique. ...https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-3-78