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*  Fluorescence recovery after photobleaching analysis aft | Open-i
Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells.LiMRE11-GFP is recruited ... pgen-1004805-g004: Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells. ... pgen-1004805-g004: Fluorescence recovery after photobleaching analysis after DNA damage induction in UV-irradiated cells. ... and fluorescence levels were too low for analysis. We then turned to a heterologous system to study LiMRE11 in vivo. DNA ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4256157_pgen.1004805.g004&req=4
*  fluorescence recovery after photobleaching
... - Prinzip von FRAP FRAP (Fluorescence Recovery after Photobleaching) bezeichnet eine ... Fluorescence loss in photobleaching - Fluorescence Loss in Photobleaching, or FLIP, is a technique in fluorescence microscopy ... Fluorescence Loss in Photobleaching - Als Fluorescence Loss in Photobleaching (FLIP) bezeichnet man eine Methode der ... fluorescence recovery after photobleaching) est une méthode utilisée en microscopie à fluorescence pour mesurer la vitesse de ...
http://en.academic.ru/dic.nsf/en_molecular_biology/2438/fluorescence
*  Sensors | Free Full-Text | Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio...
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly ... Fluorescence Recovery after Photobleaching (FRAP); diffusion; biomolecular interactions biomembranes; Fluorescence Recovery ... Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules. Gamal Rayan * , Jean- ... "Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules." Sensors 10, no. 6: ...
http://www.mdpi.com/1424-8220/10/6/5927
*  Sensors | Free Full-Text | Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio...
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly ...
http://www.mdpi.com/1424-8220/10/6/5927/notes
*  Inria - Inference of protein kinetics by stochastic modeling and simulation of fluorescence recovery after photobleaching...
A small number of in silico FRAP experiments is used to construct a mapping from FRAP recovery curves to the parameters of the ... Parameter estimates from experimental data can then be computed by applying the mapping to the observed recovery curves. A ... Fluorescence recovery after photobleaching (FRAP) is a functional live cell imaging technique that permits the exploration of ... Abstract : Motivation: Fluorescence recovery after photobleaching (FRAP) is a functional live cell imaging technique that ...
https://hal.inria.fr/hal-01096966
*  Fluorescence Recovery after Photobleaching (FRAP) Assay to ...
The generated fluorescence recovery curves were used to obtain the percentage of maximum fluorescence recovery (MFR), which ... To minimize transient overexpression artifacts, fluorescence intensity values were gathered at 36 hpi using an inverted Zeiss ... corresponds to the mobile fraction, and the half-time of maximum recovery (t1/2) values. ... we used fluorescence recovery after photobleaching (FRAP) to measure the influence that some mutations and drug treatment have ...
https://bio-protocol.org/cn/e1268
*  APS -2009 APS March Meeting - Event - Analysis of Diffusion through Dynamic Network Polymers using Multi-photon Fluorescence...
Multi-Photon Fluorescence Recovery after Photobleaching (FRAP) techniques are utilized to study small molecule transport ... and the recovery of fluorescence is studied using 2-photon fluorescence microscopy. By curve-fitting fluorescence recovery ... Analysis of Diffusion through Dynamic Network Polymers using Multi-photon Fluorescence Recovery after Photobleaching. 10:00 AM- ...
http://meetings.aps.org/Meeting/MAR09/Session/Y16.11
*  Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo...
Fluorescence Recovery After Photobleaching (FRAP) has been used extensively in the study of transport and binding in biological ... Inferring the lifetime of endosomal protein complexes by fluorescence recovery after photobleaching.. *Veronika Gousseva, May ... Fluorescence Recovery After Photobleaching (FRAP) has been used extensively in the study of transport and binding in biological ... Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo ...
https://www.semanticscholar.org/paper/Quantification-of-transport-and-binding-parameters-Kaufman-Jain/24ec8e0a2ead5d7427b58916b6378f36e222ddcd
*  Fluorescence Recovery After Photobleaching (FRAP) Group on LinkedIn
A LinkedIn group for those interested in or currently using Fluorescence Recovery After Photobleaching (FRAP) for pre-screening ... Fluorescence Recovery After Photobleaching (FRAP) Group on LinkedIn. July 31, 2017 by Lance Ramsey Leave a Comment ... has started to new LinkedIn group for those interested in or currently using Fluorescence Recovery After Photobleaching (FRAP) ...
http://blog.crystallization.formulatrix.com/fluorescence-recovery-after-photobleaching-frap-group-on-linkedin
*  Local mass transfer coefficients in bacterial biofilms using fluorescence recovery after photobleaching (frap)
... ... "Local Mass Transfer Coefficients in Bacterial Biofilms Using Fluorescence Recovery After Photobleaching (FRAP)." Progress in ... This technique is based upon the Fluorescence Return After Photobleaching (FRAP) which allows image analysis observation of the ... transport of fluorescently labeled molecules as they migrate into a micro-scale photobleached zone. The technique allowed us to ...
https://scholarworks.montana.edu/xmlui/handle/1/14262
*  Formation of a Mast Cell Synapse: FcεRI Membrane Dynamics upon Binding Mobile or Immobilized Ligands on Surfaces | The Journal...
fluorescence recovery after photobleaching. IgEAF488. Alexa 488-labeled IgE. IgEDNP. anti-DNP IgE. IgEDy520. Dy520 XL-labeled ... Fluorescence recovery after photobleaching. The mobility of IgE bound to DNP-lipid containing bilayers was measured by ... Lateral mobility in membranes as detected by fluorescence recovery after photobleaching. Biophys. J. 40: 69-75. ... fluorescence recovery after photobleaching (FRAP) using a Zeiss LSM 510META confocal microscope with a ×63 1.4 NA oil immersion ...
http://www.jimmunol.org/content/184/3/1328
*  Plus it
Fluorescence Recovery After Photobleaching. For fluorescence recovery after photobleaching (FRAP) measurements, cells were ... Bleaching of TMRM fluorescence was applied in an ∼25 μm2 square at randomly chosen regions, and fluorescence intensity was ... the rate and magnitude of fluorescence recovery was greater in the insulin-treated mitochondria (slope for the first 10 s after ... G: FRAP analysis of the mitochondrial network (top). Bleaching of TMRM fluorescence was applied in an ∼25-μm2 square at ...
http://diabetes.diabetesjournals.org/content/63/1/75
*  Connexin 36 Expression Regulates Neuronal Differentiation from Neural Progenitor Cells
Cx36 knockdown was confirmed by western blotting, immunocytochemistry and functionally by fluorescence recovery after ... photobleaching (FRAP). We found that knockdown of Cx36 in neurosphere neuronal precursors significantly reduced neuronal ... Fluorescence recovery after photobleaching Is the Subject Area "Fluorescence recovery after photobleaching" applicable to this ... Fluorescence Recovery After Photobleaching (FRAP). E18 primary striatal neurons were plated in 35 mm glass-bottomed culture ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0014746
*  Blockade of Gap Junctions In Vivo Provides Neuroprotection After Perinatal Global Ischemia | Stroke
Fluorescence Recovery After Photobleaching Demonstrates Blockade of Junctional Coupling by CBX. To directly measure the extent ... The more rapid recovery of fluorescence we observed after photobleaching in organotypic slice cultures 24 hours after ischemia ... Fluorescence Recovery After Photobleaching. Slice cultures were loaded by 45-minute application of carboxyfluorescein diacetate ... Figure 3a illustrates areas within stratum pyramidale of field CA1 subjected to fluorescence recovery after photobleaching ( ...
http://stroke.ahajournals.org/content/36/10/2232
*  Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling | Journal of Cell Science
Fluorescence recovery after photobleaching. Live-cell confocal imaging was performed on transiently transfected Rab4A-Cherry ... The motility and fluorescence recovery of endosomes is affected in FLNa-knockdown cells. (A) The track speed (left) and track ... A 100-mW LASOS argon laser, set to 25% intensity, was used to photobleach a Rab4A endosome, and recovery was monitored for 26 ... Monitoring of receptor fluorescence recovery was done for 2.5 min. The mobile fraction (MF) was estimated as MF=(Fi-Fb)/(F0-Fb ...
http://jcs.biologists.org/content/130/2/490
*  Evidence for a dual role of actin in regulating chromosome organization and dynamics in yeast | Journal of Cell Science
Fluorescence recovery after photobleaching. FRAP experiments were performed with an inverted confocal microscope UltraView ERS ... and by fluorescence recovery after photobleaching (FRAP) of a region of the nuclear envelope (Materials and Methods) in WT ... Nuclear drift was corrected by using the StackReg function in ImageJ, and fluorescence recovery was quantified using a Matlab ... As observed with fluorescence and confirmed with structured illuminated microscopy (SIM), LGN concentrated into bright discrete ...
http://jcs.biologists.org/content/129/4/681
*  Motile Properties of Vimentin Intermediate Filament Networks in Living Cells | JCB
Fluorescence recovery after photobleaching shows that bleach zones across fibrils rapidly recover their fluorescence. During ... Fluorescence Recovery After Photobleaching (FRAP). FRAP was carried out with the LSM 410 confocal microscope (Carl Zeiss, Inc. ... Fluorescence intensity measurements along a photobleached GFP-vimentin fibril. Gray-scale pixel values were determined along a ... It is also interesting that the fluorescence recovery appeared to slow down relative to controls in the presence of MT and MF ...
http://jcb.rupress.org/content/143/1/147.full
*  Vinculin phosphorylation at residues Y100 and Y1065 is required for cellular force transmission | Journal of Cell Science
Fluorescence recovery after photobleaching (FRAP) of different vinculin mutants. (A) Recovery curve of a bleached vinculin ... Fluorescence recovery after photobleaching. FRAP assays were performed with the confocal microscope (Leica) and a 20× dip-in ... Fluorescence recovery of different vinculin mutants (rescue, T12, A50I, Y100E/Y1065E, Y100F/Y1065F) after photobleaching (mean ... fluorescence recovery after photobleaching (FRAP) and actin-binding assays, we demonstrate that vinculin phosphorylation of ...
http://jcs.biologists.org/content/128/18/3435
*  Photomanipulation: Leica Microsystems
Fluorescence Recovery After Photobleaching (FRAP). Fluorescence Recovery After Photobleaching (. FRAP. ) experiments require ... For more advanced applications, use the Infinity Scanner module to perform multi-ROI high-speed photobleaching experiments. ...
https://www.leica-microsystems.com/applications/life-science/photomanipulation/
*  Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol | eLife
Using fluorescence cross-correlation spectroscopy (FCCS) and fluorescence recovery after photobleaching (FRAP) we found that ... Fluorescence recovery after photobleaching (FRAP). FRAP measurements were performed on a Zeiss LSM 510 META using a 40x 1.2 NA ... The bleaching of a complete adhesion sites was done in order to ensure that the fluorescence recovery is due to exchange of ... Each derived curve was then fitted to a mono-exponential recovery model, I(t) = M(1−e−τ·t), using the Matlab fit function (with ...
https://elifesciences.org/articles/02257
*  DOC2B Acts as a Calcium Switch and Enhances Vesicle Fusion
Fluorescence recovery after photobleaching experiments. Chromaffin cells infected with DOC2BD218,220N-EGFP or EGFP alone were ... Measurement of lateral diffusion by fluorescence photobleaching recovery. In: Weir DM, Herzenberg LA, Blackwell CC, Herzenberg ... If FRAP occurs exclusively by lateral diffusion, the characteristic fluorescence recovery time τ (t1/2 for recovery) is the ... and recovery was followed by the monitoring beam. The apparent characteristic fluorescence recovery time τ and the mobile ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC2673511/
*  Plus it
Fluorescence Recovery After Photobleaching Analysis. For fluorescence recovery after photobleaching (FRAP) analysis, HeLa cells ... Fluorescence recovery over time is expressed as a percentage of the original fluorescence prior to photobleaching (left-hand ... Bottom) Fluorescence recovery was analyzed for at least 15 cells for each cell line per experiment. ... Reduced endogenous IHG-1 expression resulted in a significant decrease in the rate of fluorescence recovery (expressed as a ...
http://diabetes.diabetesjournals.org/content/63/12/4314
*  Plus it
double-color fluorescence recovery after photobleaching. DMR. dynamic mass redistribution. DVL. Disheveled. FRAP. fluorescence ... fluorescence recovery after photobleaching (FRAP) recovery]. Images were acquired using a 40×, 1.2 numerical aperture C- ... Dual-Color Fluorescence Recovery after Photobleaching.. The procedure was essentially as described in Kilander et al. (2014b), ... Live-cell imaging experiments using fluorescence recovery after photobleaching show that human FZD4 assembles-in a DVL- ...
http://molpharm.aspetjournals.org/content/90/4/447
*  Endocytosis of the Anthrax Toxin Is Mediated by Clathrin, Actin and Unconventional Adaptors | proLékaře.cz
Using fluorescence recovery after photobleaching (FRAP), Go et al. [29] have shown that the motilities of TEM8-1 and TEM8-2 at ... Fluorescence recovery after photobleaching (FRAP). HeLa cells were seeded and transfected with GFP-tagged receptors in 35 mm ... The bleaching resulted in a ∼80% depletion of fluorescence. Recovery of fluorescence was monitored over 80 seconds with a 2 ... The fluorescence of the bleached area was normalized at each time point using a non-bleached control area. Recovery kinetics ...
https://www.prolekare.cz/casopisy/plos-pathogens/2010-3/endocytosis-of-the-anthrax-toxin-is-mediated-by-clathrin-actin-and-unconventional-adaptors-46436
*  Frontiers | Supported Lipid Bilayers of Escherichia coli Extracted Lipids and Their Calcium Dependence | Materials
... lipids were investigated with fluorescence microscopy and atomic force microscopy. Ca2+ in the aqueous phase with concentration ... lipids were investigated with fluorescence microscopy and atomic force microscopy. Ca2+ in the aqueous phase with concentration ... Fluorescence recovery after photobleaching (FRAP) processes of PLE-SLBs with [Ca2+] = (A) 2.0 mM, (B) 3.0 mM, and (C) 5.0 mM. ... was used for wide-field fluorescence and qualitative fluorescence recovery after photobleaching (FRAP) observations. AFM ...
https://www.frontiersin.org/articles/10.3389/fmats.2018.00048/full