The proliferation of normal human breast tissue implanted into athymic nude mice is stimulated by estrogen but not progesterone...
In order to resolve the question of which ovarian steroid stimulates normal human mammary epithelial cell proliferation, we ... In order to resolve the question of which ovarian steroid stimulates normal human mammary epithelial cell proliferation, we ... We conclude that E2 is sufficient to stimulate human breast epithelial cell proliferation at physiologically relevant ... We conclude that E2 is sufficient to stimulate human breast epithelial cell proliferation at physiologically relevant ...http://christie.openrepository.com/christie/handle/10541/99428
SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland | The EMBO Journal
In contrast, Sharpincpdm mammary epithelial cells transplanted in vivo into wild‐type stroma, fully repopulate the mammary ... 7Center for Molecular Biology of Inflammation, Cells‐in‐Motion Cluster of Excellence, Institute of Cell Biology, Münster ... these data imply that SHARPIN regulates the normal invasive mammary gland branching morphogenesis in an epithelial cell ... Mice lacking SHARPIN expression in all cells (Sharpincpdm), and mice with a stromal (S100a4‐Cre) deletion of Sharpin, have ...http://emboj.embopress.org/content/36/2/165
Ku80 Counters Oxidative Stress-Induced DNA Damage and Cataract Formation in the Human Lens | IOVS | ARVO Journals
The human lens epithelial cell line FHL124 is a nonvirally transformed cell line generated from human capsule-epithelial ... they can result in cell death.32 Apoptosis in the lens epithelial cell population has been linked to cataract formation.18 Cell ... Validation of Ku80 knockdown in human lens epithelial cells. Depletion of Ku80 by targeted siRNA in (A) FHL124 cells and (B) ... Validation of Ku80 knockdown in human lens epithelial cells. Depletion of Ku80 by targeted siRNA in (A) FHL124 cells and (B) ...http://iovs.arvojournals.org/article.aspx?articleid=2476145
Human Corneal Epithelial Cells Express Functional PAR-1 and PAR-2 Receptors | IOVS | ARVO Journals
Methods: Human primary corneal epithelial cells (HCEC) and the human corneal epithelial cell line HCE-T were cultured to 80% ... Cells treated with PMA (50ng/ml) and TNF-α-treated (10ng/ml) cells served as positive controls for these studies. PAR-1 and PAR ... R Lang, B Harten, H Kalden, FJ Legat, PI Song, NW Bunnett, CA Armstrong, JC Ansel; Human Corneal Epithelial Cells Express ... Human Corneal Epithelial Cells Express Functional PAR-1 and PAR-2 Receptors ...http://iovs.arvojournals.org/article.aspx?articleid=2417632
Role of β-Pix in Corneal Epithelial Cell Migration on Fibronectin | IOVS | ARVO Journals
HCE cells were plated at a density of 2 × 104 cells per 35-mm dish, 5 × 105 cells per 100-mm dish, or 5 × 104 cells per well in ... A role for β-Pix in cell migration has been demonstrated in various cell types. 13,27 -29 Although HCE cells have been ... Upregulation of ZO-1 in Cultured Human Corneal Epithelial Cells by a Peptide (PHSRN) Corresponding to the Second Cell-Binding ... Epidermal growth factor stimulates integrin-mediated cell migration of cultured human corneal epithelial cells on fibronectin ...http://iovs.arvojournals.org/article.aspx?articleid=2165675
Role of C/EBPβ in two luminal progenitor populations in the mouse mammary gland
The mammary gland is a branched epithelial organ comprised of myoepithelial, ductal and alveolar cells that are derived from ... The progression from mammary gland stem cell(s) to the differentiated mammary gland cell types is poorly understood. Here, I ... In Chapter 2, I describe the isolation of two luminal progenitor cell populations (Sca1+ and Sca1- luminal cells) and show that ... and that C/EBPβ target genes perform distinct cellular functions in alveolar cells (Sca1- cells and P16.5). Furthermore, I show ...https://www.era.lib.ed.ac.uk/handle/1842/9522
Exclusion of a Proton ATPase from the Apical Membrane Is Associated with Cell Polarity and Tip Growth in Nicotiana tabacum...
Adams, C.L., Chen, Y.-T., Smith, S.J., and James Nelson, W. (1998). Mechanisms of epithelial cell-cell adhesion and cell ... A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells. Cell 125: 535-548. ... in low-density MDCK cells (Jesaitis and Yguerabide, 1986) and E-cadherin in epithelial cells before adhesion (Adams et al., ... In Methods in Cell Biology. A Practical Guide to the Study of Ca2+ in Living Cells, R. Nuccitelli, ed (San Diego, CA: Academic ...http://www.plantcell.org/content/20/3/614.full
Laminin and tenascin assembly and expression regulate HC11 mouse mammary cell differentiation | Journal of Cell Science
HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond ... Building a plant cell wall. In their Cell Science at a Glance article and accompanying poster, Staffan Persson and colleagues ... Special issue on plant cell biology. We've just published our special issue focussing on plant cell biology - check out the ... on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at ...http://jcs.biologists.org/content/107/4/1031
Vacuolar ATPase Regulates Surfactant Secretion in Rat Alveolar Type II Cells by Modulating Lamellar Body Calcium
How surfactant is released from alveolar epithelial type II cells is not fully understood. Vacuolar ATPase (V-ATPase) is the ... Proteomic analysis revealed that vacuolar ATPase (V-ATPase) dominated the alveolar type II cell lipid raft proteome. Western ... Stimulation of type II cells with surfactant secretagogues dissipated the pH gradient across lamellar bodies and disassembled ... Cell staining Is the Subject Area "Cell staining" applicable to this article? Yes. No. ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0009228
Expression of SPARC in Murine Lens | IOVS | ARVO Journals
These findings implicate SPARC in the maintenance of the functions and activities of lens epithelial cells. The lack of SPARC ... By immunohistochemistry and RT-PCR, the lens fiber cells at the equator were shown to contain SPARC, but not the secondary ... steady-state levels of SPARC mRNA in lens cells were determined by RT-PCR. Results: Lens epithelium produced abundant SPARC as ... Immunohistochemistry and immunoblot analyses were conducted on murine lens capsule and on lens cells from E14 to 2 yr; ...http://iovs.arvojournals.org/article.aspx?articleid=2420794
SCRIBBLE is required for pregnancy-induced alveologenesis in the adult mammary gland | Journal of Cell Science
Unlike its role in virgin gland as a negative regulator cell proliferation, SCRIB is a positive regulator of mammary epithelial ... SCRIB was required in the epithelial cell compartment of the mammary gland. Lack of SCRIB attenuated prolactin-induced ... The cell polarity protein SCRIB is a critical regulator of polarization, cell migration and tumourigenesis. Whereas SCRIB is ... The splicing co-factor Barricade/Tat-SF1 is required for cell cycle and lineage progression in Drosophila neural stem cells. ...http://jcs.biologists.org/content/early/2016/05/12/jcs.185413
C57BL/6 Mouse Colonic Smooth Muscle Cells | Creative Bioarray
C57BL/6 Mouse Primary Mammary Epithelial Cells *C57BL/6 Mouse Vein Endothelial Cells ... PRODUCTS Cells. Probe. BioBank. Tissue Array SERVICES Cell Services. Cell-Based Drug Discovery Services. Histology Services. ... Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by ... Each vial contains at least 1x10^6 cells per ml and is delivered frozen.. Mouse Colonic Smooth Muscle Cells are characterized ...https://www.creative-bioarray.com/C57BL-6-Mouse-Colonic-Smooth-Muscle-Cells-CSC-C4292X-item-2567.htm
Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability, migration and proliferation...
In corneal epithelial cell cultures, fetal bovine serum (FBS) is often used to support the growth of the cells. The dose- ... Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability, migration and proliferation ... Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability, migration and proliferation ... dependent effect of AS and FBS on viability, migration and proliferation of human corneal epithelial cells (HCECs) have not ...http://iovs.arvojournals.org/article.aspx?articleid=2335673
The in vivo function of mammalian cell and tissue polarity regulators - how to shape and maintain the epidermal barrier |...
2009). The Crumbs complex: from epithelial-cell polarity to retinal degeneration. J. Cell Sci. 122, 2587-2596. doi:10.1242/jcs. ... 2010). Stem cell dynamics in mouse hair follicles: a story from cell division counting and single cell lineage tracing. Cell ... Regulation of cell fate and stem cell behavior in the epidermis. Cell division not only generates daughter cells, but through ... 2008). Regulation of cell polarity during epithelial morphogenesis. Curr. Opin. Cell Biol. 20, 227-234. doi:10.1016/j.ceb. ...http://jcs.biologists.org/content/125/15/3501
Model for different phases of mammary gland branching m | Open-i
Before puberty, the mammary epithelial is small and simply branched. In response to the rel ... Although it is likely that cell migration is important for branching and the invasion of the epithelial cell layer into the fat ... Although it is likely that cell migration is important for branching and the invasion of the epithelial cell layer into the fat ... MMP-2, but not MMP-9, facilitates terminal end bud invasion by inhibiting epithelial cell apoptosis at the start of puberty. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2172848_200302090f6&req=4
Mechanisms of HERV-K (HML-2) transcription during human mammary epithelial cell transformation. - Semantic Scholar
We saw active 5' LTR use in tumorigenic cells only, suggesting that the cellular environment of a cancer cell is a critical ... human mammary epithelial cell transformation to assess how malignancy-associated shifts in the transcriptional milieu of a cell ... we characterized the HML-2 transcriptome and means of activation in an in vitro model of human mammary epithelial cell ... Importantly, LTR-driven transcription was restricted to tumorigenic cells, suggesting that LTR promoter activity is dependent ...https://www.semanticscholar.org/paper/Mechanisms-of-HERV-K-HML-2-transcription-during-hu-Montesion-Bhardwaj/0ff59b0096696526bdffd5d0a79865ea188a5bbc
Hyperosmolarity Stimulates Production of MMP-9, IL-1ß and TNF- by Human Corneal Epithelial Cells Via a c-Jun NH2-terminal...
... in human corneal epithelial cells (HCE) in response to hyperosmotic stress. Methods: Primary human corneal epithelial cells ... Hyperosmolarity Stimulates Production of MMP-9, IL-1ß and TNF- by Human Corneal Epithelial Cells Via a c-Jun NH2-terminal ... Hyperosmolarity Stimulates Production of MMP-9, IL-1ß and TNF- by Human Corneal Epithelial Cells Via a c-Jun NH2-terminal ... by Human Corneal Epithelial Cells Via a c-Jun NH2-terminal Kinase Pathway . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1981. ...http://iovs.arvojournals.org/article.aspx?articleid=2419244
Expression of estrogen receptors in a normal human breast epithelial cell type with luminal and stem cell characteristics and...
In this report, we studied the expression of ER in these two types of cells and their transformed cell lines. The results ... In this report, we studied the expression of ER in these two types of cells and their transformed cell lines. The results ... only a small proportion of epithelial cells in the mammary gland express the ER. The origin of the ER-positive breast cancers ... only a small proportion of epithelial cells in the mammary gland express the ER. The origin of the ER-positive breast cancers ...http://oxfordindex.oup.com/view/10.1093/carcin/18.2.251
State-dependent inactivation of K+ currents in rat type II alveolar epithelial cells. | JGP
State-dependent inactivation of K+ currents in rat type II alveolar epithelial cells.. T E DeCoursey ... Inactivation of K+ channels responsible for delayed rectification in rat type II alveolar epithelial cells was studied in ... State-dependent inactivation of K+ currents in rat type II alveolar epithelial cells. ...http://jgp.rupress.org/content/95/4/617
Genome-wide Identification Of Genes And MicroRNAs Regulated By Fgf2 During In Vitro Lens Fiber Cell Differentiation | IOVS |...
We employed a rat lens epithelial explant system and performed integrated RNA and microRNA expression profiling in cells ... cell death, cell proliferation, cell junction and protein serine/threonine kinase activity. The genes regulated by multiple ... In lens, FGF signaling was implicated as the key pathway that controls lens fiber cell differentiation, but little is known ... Role of microRNAs during lens fiber cell differentiation was assessed in vivo through conditional inactivation of Dicer using ...http://iovs.arvojournals.org/article.aspx?articleid=2360607
Diet-induced obesity disrupts ductal development in the mammary glands of nonpregnant mice - Kamikawa - 2009 - Developmental...
Cell Proliferation Assay With Bovine Mammary Epithelial Cells (BMECs). BMECs were prepared as described previously (Yamaji et ... Leptin enhances STAT-3 phosphorylation in HC11 cell line: effect on cell differentiation and cell viability. Mol Cell ... inhibition of cell proliferation is also reported in noncancerous mouse mammary epithelial cell lines as well as bovine cells ( ... an epithelial marker. To further enrich the epithelial cell population, BMECs were embedded in a type I collagen gel (0.6 mg/ml ...http://onlinelibrary.wiley.com/doi/10.1002/dvdy.21947/full
BALB/c Mouse Primary Mammary Epithelial Cells | Creative Bioarray
Mouse Primary Mammary Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical ... PRODUCTS Cells. Probe. BioBank. Tissue Array SERVICES Cell Services. Cell-Based Drug Discovery Services. Histology Services. ... Mouse Primary Mammary Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution ... Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is ...https://www.creative-bioarray.com/BALB-c-Mouse-Primary-Mammary-Epithelial-Cells-CSC-C4316X-item-2591.htm
Epithelial Cell Culture Protocols | SpringerLink
In Epithelial Cell Culture Protocols, a team of well-versed experimenters and cli ... Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. ... and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use ... Applications of Epithelial Cell Culture in Studies of Drug Transport Staffan Tavelin, Johan Gråsjö, Jan Taipalensuu, Göran ...https://link.springer.com/book/10.1385/159259185X
What Are Epithelial Cells? | Reference.com
Epithelial cells are the cells that make up the skin and other sections of the body, such as the throat and the outside of ... Epithelial cells are the cells that make up the skin and other sections of the body, such as the throat and the outside of ... How Are Cells Organized?. A: Single-celled organisms are organized using specialized cell organelles, while several cells may ... What Structures Do Human Epithelial Cells Have in Common With Elodea Cells?. ...https://www.reference.com/science/epithelial-cells-33f43de5c93224f
Epithelial Cells: Immunological Aspects
... epithelial cells play an active role in the generation and expression of protective immune responses ... inflammatory signals in epithelial cells, in turn responsible for further recruitment and local activation of immune cells, ... dendritic cells (DC) and T cells. In turn, the cytokines released at high levels by infiltrating T cells, mainly represented by ... including epidermal stem cells. Terminal differentiation begins when basal cells withdraw from the cell cycle and lose their ...http://www.els.net/WileyCDA/ElsArticle/refId-a0001127.html