New insights into the role of EMT in tumor immune escape - Terry - 2017 - Molecular Oncology - Wiley Online Library
2008) The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell 133, 704-715. ... 7 Alterations of cell-cell interactions and immunological synapses. Immune killer cells, such as CTLs and NK cells, highly rely ... 2012) Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and - ... Cross-talks between carcinoma cells, immunosuppressive cells, and immune effector cells within the tumor microenvironment in ...
Mechanistic insights into p53‐regulated cytotoxicity of combined entinostat and irinotecan against colorectal cancer cells -...
2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barretts epithelium. ... 2.1 Cell culture. HCT116 cells were a gift from B. Vogelstein (Baltimore, USA). H1299 cells with inducible p53wt or p53K120R ... J Cell Biol 93, 103-110. *32Poburski D & Thierbach R (2016) Improvement of the BALB/c-3T3 cell transformation assay: a tool for ... cells were grown in T25 cell culture flasks and DMEM containing 1 µg·mL−1 puromycin. After 6 weeks of selection, cells stably ...
Identifying Cell‐Type‐Specific Metabolic Signatures Using Transcriptome and Proteome Analyses - Gebert - 2021 - Current...
Abstract Studies in various tissues have revealed a central role of metabolic pathways in regulating adult stem cell function ... For single-cell RNA-seq, sort 50,000 live (7AAD-) epithelial cells (Epcam+) into a 1.5-ml microcentrifuge tube containing 50 μl ... 6. Sort cells by FACS. *Using the FACSAria3 cell sorter for bulk RNA-seq, sort 25,000 live cells (7AAD−) per population (i.e., ... Perez-Ramirez, C. A., & Christofk, H. R. (2021). Challenges in studying stem cell metabolism. Cell Stem Cell, 28(3), 409-423. ...
Lungs of patients with idiopathic pulmonary alveolar proteinosis express a factor which neutralizes granulocyte‐macrophage...
Non-adherent cells were removed by vigorous washing with PBS and adherent cells were collected by gentle scraping using a cell ... Furthermore, Huffmann and colleagues recently reported that pulmonary epithelial expression of GM-CSF using an SP-C-GM-CSF ... The viability of cells was determined by the trypan blue dye exclusion. TF-1 is a cell line dependent on GM-CSF or IL-3 [16]. ... The cell suspensions were centrifuged at 250×g for 5 min to remove unbound 125I-GM-CSF from the cells, washed three times with ...