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*  The Effect of Doxorubicin on Viability and Morphology of Human Embryonic Stem Cell-derived Cardiomyocytes
In spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. Isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be passaged as a cell line. In the present study, human embryonic stem cell (hESC)-derived cardiomyocytes applied as a model for evaluation of doxorubicin cardiotoxicity. In this process, cardiogenic differentiated hESCs spheroids were exposed to different concentrations of doxorubicin for 24, 48 and 72 hours. The viability of spheroids as well as their morphology was assessed as important criterion of cardiotoxicity. Findings of the study showed that the viability of spheroids was significantly reduced at doses of 3 and 30 µM (P|0.05). Moreover, cell morphology was changed in the presence ...
http://jchr.sinaweb.net/article_531947.html
*  Patente US20150056698 - Primate embryonic stem cells - Google Patentes
A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
http://www.google.es/patents/US20150056698?hl=es&dq=flatulence
*  Embryonic Stem Cell Marker Antibody Panels from R&D Systems
... ,R&D Systems adds two new Stem Cell Antibody Panels to its expanding line of stem cell products. The Human Embryonic Stem Cell Marker Antibody Panel (Catalog # SC008) contains the antibodies against: alkaline phosphatase, Nanog, Oct-3/4, SSEA-1 and SSEA-4. The Human Embryonic Stem Cell Marker Antibo,biological,biology supply,biology supplies,biology product
http://www.bio-medicine.org/biology-products/Embryonic-Stem-Cell-Marker-Antibody-Panels-from-R-26D-Systems-3884-1/
*  Inhibition of Mitochondrial Complex III Blocks Neuronal Differentiation and Maintains Embryonic Stem Cell Pluripotency
The mitochondrion is emerging as a key organelle in stem cell biology, acting as a regulator of stem cell pluripotency and differentiation. In this study we sought to understand the effect of mitochondrial complex III inhibition during neuronal differentiation of mouse embryonic stem cells. When exposed to antimycin A, a specific complex III inhibitor, embryonic stem cells failed to differentiate into dopaminergic neurons, maintaining high Oct4 levels even when subjected to a specific differentiation protocol. Mitochondrial inhibition affected distinct populations of cells present in culture, inducing cell loss in differentiated cells, but not inducing apoptosis in mouse embryonic stem cells. A reduction in overall proliferation rate was observed, corresponding to a slight arrest in S phase. ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082095
*  Calcium Handling in Human Embryonic Stem Cell-Derived Cardiomyocytes - Satin - 2008 - STEM CELLS - Wiley Online Library
Peppiatt CM, Collins TJ, Mackenzie L et al. 2-Aminoethoxydiphenyl borate (2-APB) antagonises inositol 1,4,5-trisphosphate-induced calcium release, inhibits calcium pumps and has a use-dependent and slowly reversible action on store-operated calcium entry channels. Cell Calcium 2003; 34:97-108 ...
http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2007-0591/references
*  System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation |...
To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of hESCs during differentiation initiated by a diacylglycerol analog or transfer to media that had not been conditioned by feeder cells. We profiled 6521 proteins and 23,522 phosphorylation sites, of which almost 50% displayed dynamic changes in phosphorylation status during 24 hours of differentiation. These data are a resource for studies of the events associated with the maintenance of hESC pluripotency and those accompanying their differentiation. From these data, we identified a core hESC phosphoproteome of sites with similar robust changes in response to the two distinct treatments. These sites exhibited distinct dynamic phosphorylation patterns, which were linked to known or predicted kinases on the basis of the matching sequence motif. In addition to identifying ...
http://stke.sciencemag.org/content/4/164/rs3
*  Epithelial-mesenchymal transition events during human embryonic stem cell differentiation - The Christie Research Publications...
Epithelial-mesenchymal transition (EMT) occurs during embryonic development and may also be associated with the metastatic spread of epithelial tumors. During EMT, E-cadherin is down-regulated and this correlates with increased motility and invasion of cells. We show that differentiation of human embryonic stem (ES) cells in monolayer culture is associated with an E- to N-cadherin switch, increased vimentin expression, up-regulation of E-cadherin repressor molecules (Snail and Slug proteins), and increased gelatinase (matrix metalloproteinases; MMP-2 and MMP-9) activity and cellular motility, all characteristic EMT events. The 5T4 oncofetal antigen, previously shown to be associated with early human ES cell differentiation, is also part of this process. Abrogation of E-cadherin-mediated cell-cell contact in undifferentiated ES cells using neutralizing antibody (nAb) SHE78.7 resulted in increased cellular ...
http://christie.openrepository.com/christie/handle/10541/71934
*  Mouse Embryonic Stem Cell Derivation, and Mouse and Human Embryonic Stem Cell Culture and Differentiation as Embryoid Bodies -...
Embryonic stem (ES) cells are pluripotent cells derived from developing mouse blastocysts in vitro that maintain long‐term self renewal and the capacity to give rise to all cell types in the adult body (including some extraembryonic cell types) when subjected to the appropriate conditions
http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb2302.html
*  Research on Human Embryonic Stem Cells Marks 10-Year Milestone
Sept. 14, 1999: The Wisconsin Alumni Research Foundation establishes WiCell as a clearinghouse to distribute stem cells and foster research.. -- Aug. 9, 2001: President Bush announces his decision to limit federal funding for embryonic stem cell research to cell lines in existence at that point in time.. -- Sept. 4, 2001: A team of Wisconsin scientists led by Dan Kaufman announces it has coaxed stem cells to become blood cells.. -- Nov. 30, 2001: Neural progenitor cells, stem cells that have migrated part way down the developmental pathway to becoming specific types of brain cells, are created and implanted in mice where the cells further develop into functioning neurons. The work was conducted in the laboratory of UW-Madison stem cell scientist Su-Chun Zhang at the Waisman Center.. -- Feb. ...
http://www.innovations-report.com/html/reports/life-sciences/research-human-embryonic-stem-cells-marks-10-year-121913.html
*  Effects of hydrodynamic culture on embryonic stem cell differentiation: cardiogenic modulation - pdf descargar
Effects of hydrodynamic culture on embryonic stem cell differentiation: cardiogenic modulation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
http://libros.duhnnae.com/2017/jun8/149822029832-Effects-of-hydrodynamic-culture-on-embryonic-stem-cell-differentiation-cardiogenic-modulation.php
*  Human Embryonic Stem Cells
www.MOLUNA.de Human Embryonic Stem Cells [4221481] - Part I. PolicynEthical Issues Associated with Pluripotent Stem CellsnSteve UsdinnA Researcher's Guide to Federally Funded Human Embryonic Stem Cell Research in the United StatesnGregory J. DowningnIntellectual Property of Human Pluripotent Stem CellsnMark L. RohrbaughnPart II. Types of Pluripotent CellsnEmbryonal Carcinoma Cells: The Malignant Counterparts of ES and EG CellsnJonathan S.
https://www.moluna.de/buch/4221481-human+embryonic+stem+cells/
*  Lokaverkefni: "The effect of Dlg7 overexpression and silencing on mouse embryonic stem cell differentiation" | Skemman
Dlg7, a novel cell cycle regulator gene, is expressed in stem cells, including haematopoietic stem cells (HSC), mesenchymal stem cells (MSC) and mouse embryonic stem cells (mESC). Dlg7 is expressed in human haematopoietic stem cells (CD34+CD38-) but much less expression was detected in haematopoietic progenitor cells (CD34+38+) or in fully mature blood cells. Dlg7 is expressed in many leukemic cell lines and in several tumours including bladder, colon and liver, while not being found in healthy adjacent normal tissue. In addition, it is a potential oncogenic target of Aurora-A, and is associated with invasiveness of hepatocellular carcinoma ...
https://skemman.is/handle/1946/7527
*  Increase in Human Embryonic Stem Cell Research Helps Future of Industry - Cell Culture Dish
Since President Obama repealed research restrictions on human embryonic stem cells (hESCs) in 2009, hESC research has expanded significantly. Before the repeal there were 20 hESC-endorsed lines available for government research funding, now there are 128.. While government funding has been more accessible, investors have still been leery of investing in stem cell companies that use hESCs. There is still a great deal of concern regarding the political environment for this kind of research and many are concerned that if President Obama does not get reelected in 2012 that government funding will again be drastically reduced and that many hESC companies will not be able to survive the cuts. Although the increase in hESC lines available for government funded research has given a boost to the industry, other non-embryonic stem cell work is still funded at a significantly higher rate. According to a recent article ...
http://cellculturedish.com/2011/08/increase-in-human-embryonic-stem-cell-research-helps-future-of-industry/
*  Stem cells: What they are and what they do - Mayo Clinic
Embryonic stem cells are obtained from early-stage embryos - a group of cells that forms when a woman's egg is fertilized with a man's sperm in an in vitro fertilization clinic. Because human embryonic stem cells are extracted from human embryos, several questions and issues have been raised about the ethics of embryonic stem cell research. The National Institutes of Health created guidelines for human stem cell research in 2009. Guidelines included defining embryonic stem cells and how they may be used in research and donation guidelines for embryonic stem cells. Also, guidelines stated embryonic stem cells may only be used from embryos created by in vitro fertilization when the embryo is ...
https://www.mayoclinic.org/tests-procedures/stem-cell-transplant/in-depth/stem-cells/art-20048117?pg=2&p=1
*  FAQ about Custom Stem Cell-derived Endothelial Cell Isolation
Primary stem cell-derived endothelial cells can be used for a variety of purposes (e.g., assays of cell-cell adhesion, migration, vascular tube formation, angiogenesis assays and many other applications) Standard biochemical procedures can be performed using endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent staining or flow cytometry, et al.. Primary stem cell-derived endothelial cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use. Transfer or resale of any Cell Biologics' cells or products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics without the express written consent of the company. Cell Biologics' Terms and Conditions must be accepted before submitting an order.. Question 9: How much does isolation of stem cell-derived endothelial ...
http://cellbiologics.com/index.php?route=information/information&information_id=16
*  Hepatocyte-like cell differentiation from human embryonic stem cells - Differentiation protocol - LifeMap Discovery
This protocol describes highly efficient means of deriving functional human hepatocyte-like cells from hESCs. The significance of this in vitro technology is the ability to generate homogeneous and limitless cultures of high fidelity human hepatocyte-like cells for applied biology. In this protocol, no functional assays such as detoxification, synthesis of clotting factors, metabolism of ammonia or excretion of bilirubin, have been shown.. ...
https://discovery.lifemapsc.com/stem-cell-differentiation/protocols/liver-differentiation-hepatocyte-like-cell-differentiation-from-human-embryonic-stem-cells?TCCellID=fdafa7c2-fec8-45e6-8436-f1523c577ea7
*  Use of human embryonic stem cells to model pediatric gliomas with H3.3K27M histone mutation. - Semantic Scholar
Over 70% of diffuse intrinsic pediatric gliomas, an aggressive brainstem tumor, harbor heterozygous mutations that create a K27M amino acid substitution (methionine replaces lysine 27) in the tail of histone H3.3. The role of the H3.3K27M mutation in tumorigenesis is not fully understood. Here, we use a human embryonic stem cell system to model this tumor. We show that H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation. Genome-wide analyses indicate a resetting of the transformed precursors to a developmentally more primitive stem cell state, with evidence of major modifications of histone marks at several master regulator genes. Drug screening assays identified a compound targeting the protein menin as an inhibitor of tumor cell growth ...
https://www.semanticscholar.org/paper/Use-of-human-embryonic-stem-cells-to-model-pediatr-Funato-Major/28bd115608ab9cd001e088e50d292ede3b828c21
*  Differentiation of human embryonic stem cells to corneal epithelium and endothelium like cells for cornea replacement...
Purpose: A potential application of human embryonic stem cells (hESCs) is the generation of corneal epithelial (CEpi) and endothelial (CEndo) cells to be used for corneal disease treatment. In this study, we designed a new method to induce hESC differentiating to CEpi and CEndo like cells for cornea substitute construction.. Methods: Human embryonic stem cells (hESC) were cultured in a transwell coculture system with differentiated human corneal stromal cells to differentiate into periocular mesenchymal precursor (POMPs). Next, the CEndo-like cells expressed N-cadherin/vimention were derived from POMPs with lens epithelial cell-conditioned medium, and isolated by Fluorescence-activated cell sorting (FACS). To obtain CEpi-like cells, the hESCs were cultured in the limbal stem ...
http://iovs.arvojournals.org/article.aspx?articleid=2335906
*  Epigenetic plasticity of cultured female human embryonic stem cells and regulation of gene expression and chromatin by PR-SET7...
Epigenetics is the study of changes in gene expression that occur in cells without alterations to DNA sequence. Epigenetic modifications are critical components of eukaryotic gene regulation and chromatin organization. Different epigenetic mechanisms, including the post-translational modifications of DNA-associated histone proteins play a role in the activation or repression of genes. ❧ One of my research goals was to define the epigenetic signature of cultured human embryonic stem cells (hESCs) and to determine how their epigenomes change during lineage commitment. Pluripotent hESCs are capable of self-renewal and have the capacity to differentiate into any lineage of the embryo. However, hESCs grown in culture are heterogeneous in nature, consisting of a mixture of pluripotent to differentiated cells, making investigation of pluripotent hESCs difficult. Therefore precise definition of pluripotent cells ...
http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/112300/rec/11
*  Epigenetic plasticity of cultured female human embryonic stem cells and regulation of gene expression and chromatin by PR-SET7...
Epigenetics is the study of changes in gene expression that occur in cells without alterations to DNA sequence. Epigenetic modifications are critical components of eukaryotic gene regulation and chromatin organization. Different epigenetic mechanisms, including the post-translational modifications of DNA-associated histone proteins play a role in the activation or repression of genes. ❧ One of my research goals was to define the epigenetic signature of cultured human embryonic stem cells (hESCs) and to determine how their epigenomes change during lineage commitment. Pluripotent hESCs are capable of self-renewal and have the capacity to differentiate into any lineage of the embryo. However, hESCs grown in culture are heterogeneous in nature, consisting of a mixture of pluripotent to differentiated cells, making investigation of pluripotent hESCs difficult. Therefore precise definition of pluripotent cells ...
http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/112300/rec/512
*  The Strata-Sphere » Grasping At A Deadly, Evil Folly
I'm going to venture in here and throw in my two cents in hopes that you wrote this piece in earnest, not realizing the scientific implications of your accusations and postulations. However, I must say, first and foremost, for the integrity of this piece and any others you've written on human embryonic stem cells, you are not a scientist. As such, it is not only unfair for you to make assumptions, but what's worse is that you are actually distributing your opinion as fact. Describing human embryonic stem cell research as "the killing of young human beings for spare parts" is not only inaccurate, but entirely untrue. It is because of people like yourself that human embryonic stem cells have become synomous with the idea of scientists growing a baby 'matrix-style' and carving it up into little pieces, throwing away the rest. This is NOT what human embryonic ...
http://strata-sphere.com/blog/index.php/archives/7503
*  Establishing and optimizing feeder cell-free culture methods for human embryonic stem cells
Background and Aims: Human embryonic stem cells (hESCs) are pluripotent cells and thus provide a promising cell source for clinical applications of regenerative medicine. Currently hESCs are cultured on fibroblast feeder cell layers, which provide necessary cell-cell interactions for the attachment and soluble factors enabling the undifferentiated growth of hESCs. However, culturing of feeder cells is expensive and laborious. In addition, xeno-products, used in feeder cell and hESC cultures could transmit animal pathogens to hESCs, and cause rejections when transplanted to patients. Therefore there is a need to develop xeno- and feeder cell-free culturing methods for hESCs. The first aim of this research project was to set up and compare two commercial xeno-products containing feeder cell-free culturing methods for hESCs. The second aim was to optimize a novel, defined, serum- and xeno-free Reges medium, developed in Regea, ...
http://tampub.uta.fi/handle/10024/81021
*  U-M human embryonic stem cell line placed on national registry - Healthcanal.com : Healthcanal.com
ANN ARBOR, Mich. - The University of Michigan's first human embryonic stem cell line will be placed on the U.S. National Institutes of Health's registry, making the cells available for federally-funded research. It is the first of the stem cell lines derived at the University of Michigan to be placed on the registry.. The line, known as UM4-6, is a genetically normal line, derived in October 2010 from a cluster of about 30 cells removed from a donated five-day-old embryo roughly the size of the period at the end of this sentence. That embryo was created for reproduction but was no longer needed for that purpose and was therefore about to be discarded.. "This is significant, because acceptance of these cells on the registry demonstrates our attention to details of proper oversight, consenting, and following of NIH guidelines established in 2009," says Gary Smith, Ph.D., who derived the line and also is ...
https://www.healthcanal.com/public-health-safety/26603-u-m-human-embryonic-stem-cell-line-placed-on-national-registry.html
*  Natural Scaffolds for Renal Differentiation of Human Embryonic Stem Cells for Kidney Tissue Engineering
Despite the enthusiasm for bioengineering of functional renal tissues for transplantation, many obstacles remain before the potential of this technology can be realized in a clinical setting. Viable tissue engineering strategies for the kidney require identification of the necessary cell populations, efficient scaffolds, and the 3D culture conditions to develop and support the unique architecture and physiological function of this vital organ. Our studies have previously demonstrated that decellularized sections of rhesus monkey kidneys of all age groups provide a natural extracellular matrix (ECM) with sufficient structural properties with spatial and organizational influences on human embryonic stem cell (hESC) migration and differentiation. To further explore the use of decellularized natural kidney scaffolds for renal tissue engineering, pluripotent hESC were seeded in whole- or on sections of kidney ECM and cell migration and phenotype compared with the established ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143849