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*  DNase I Footprinting Assay Service - Profacgen
Profacgen provides professional DNase I footprinting assay service for the identification of exact binding sites of DNA-binding ... Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis , Electrophoretic Mobility Shift Assay ( ... Electrophoretic Mobility Shift Assay (EMSA) DNase I Footprinting Assay Chromatin Immunoprecipitation Assay (ChIP) ... it is recommended to be performed after interaction confirmation by Electrophoretic Mobility Shift Assay (EMSA). Therefore, in ...
https://www.profacgen.com/dnase-i-footprinting-assay-service.htm
*  THE INTERPLAY AMONG NF-Y AND E-BOX TRANSCRIPTION FACTORS: MAX, MYC AND USF1 | Archivio Istituzionale della Ricerca
Myc or USF1 using Electrophoretic Mobility Shift Assay (EMSA) and ChIP analyses: in vitro EMSAs were performed using E-BOX TFs ...
https://air.unimi.it/handle/2434/274117
*  Electrophoretic Mobility Shift Assay (EMSA) | Protocol (Translated to German)
Die elektrophoretische Mobilität Shift-Assay (EMSA) ist eine biochemische Verfahren zur Bindung zwischen Proteinen und ... Electrophoretic Mobility Shift Assay (EMSA) für das Studium von RNA-Protein-Wechselwirkungen: Die IRE / IRP Beispiel Carine ... Elektrophoretische Mobilität Shift Assay (EMSA). USE JoVE IN YOUR CLASSROOM CREATE A JoVE TEST ... EMSA, elektrophoretische Mobilität-Shift-Test, auch bekannt als das Gel Schicht Assay, ist ein vielseitiger und sensible ...
https://www.jove.com/science-education/5694/elektrophoretische-mobilitt-shift-assay-emsa?language=German
*  Electrophoretic Mobility-Shift Assay (EMSA) Kit, with SYBR Green SYPRO Ruby EMSA stains
This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect ... This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect ... Electrophoretic Mobility-Shift Assay (EMSA) Kit, with SYBR™ Green & SYPRO™ Ruby EMSA stains ... Electrophoretic Mobility-Shift Assay (EMSA) Kit, with SYBR™ Green & SYPRO™ Ruby EMSA stains. ...
https://www.thermofisher.com/order/catalog/product/E33075
*  Difference between revisions of "Knight:Electrophoretic mobility shift assay" - OpenWetWare
A sensitive two-color electrophoretic mobility shift assay for detecting both nucleic acids and protein in gels. Proteomics. ... The utility of a two-color fluorescence electrophoretic mobility shift assay procedure for the analysis of DNA replication ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Knight:Electrophoretic_mobility_shift_assay&oldid=70953" ...
https://openwetware.org/wiki/?title=Knight:Electrophoretic_mobility_shift_assay&diff=70953&oldid=70949
*  A Proteomics Approach for the Identification of DNA Binding Activities Observed in the Electrophoretic Mobility Shift Assay |...
A Proteomics Approach for the Identification of DNA Binding Activities Observed in the Electrophoretic Mobility Shift Assay. ... We present here a novel method for the identification of DNA-binding proteins seen in electrophoretic mobility shift assay ( ... DNase I footprinting and electrophoretic mobility shift assays (EMSA)1 are generally employed for the rapid characterization of ... A Proteomics Approach for the Identification of DNA Binding Activities Observed in the Electrophoretic Mobility Shift Assay ...
http://www.mcponline.org/content/1/6/472.full
*  Active Motif » Gelshift Chemiluminescent EMSA Assay (gel shift, electrophoretic mobility shift assay, gel retardation, non...
The Gelshift Chemiluminescent EMSA Assay Kit simplifies the process of identifying protein:DNA binding interactions by ... providing an easy-to-use, non-radioactive assay with proven reagents. ... Electrophoretic mobility shift assays (EMSA), also known as gel shifts, gel retardation assays or mobility assays can be used ... non-radioactive assay to identify protein-DNA binding with proven reagents. In this electrophoretic mobility shift assay (EMSA ...
https://www.activemotif.com/catalog/648/gelshift-chemiluminescent-emsa
*  Electrophoretic Mobility Shift Assay (EMSA) Service - Profacgen
Profacgen offers professional Electrophoretic Mobility Shift Assay (EMSA) service to help your study on DNA-protein interaction ... Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis , Electrophoretic Mobility Shift Assay ( ... Electrophoretic mobility shift assay (EMSA, also called gel retardation assay or gel shift assay) is an in vitro method to ... Electrophoretic Mobility Shift Assay (EMSA) DNase I Footprinting Assay Chromatin Immunoprecipitation Assay (ChIP) ...
https://www.profacgen.com/Electrophoretic-Mobility-Shift-Assay-EMSA-Service.htm
*  AID 1260275 - Inhibition of recombinant human PI3Ka using fluorescein-labeled peptide as substrate by fluorescence...
Inhibition of recombinant human PI3Ka using fluorescein-labeled peptide as substrate by fluorescence-electrophoretic mobility ... shift assay. ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/1260275
*  AID 1260248 - Inhibition of recombinant human c-Raf using fluorescein-labeled peptide as substrate by fluorescence...
Inhibition of recombinant human c-Raf using fluorescein-labeled peptide as substrate by fluorescence-electrophoretic mobility ... shift assay. ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/1260248
*  Electrophoretic mobility shift assay - Wikipedia
... or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel ... A mobility shift assay is electrophoretic separation of a protein-DNA or protein-RNA mixture on a polyacrylamide or agarose gel ... Hellman, Lance M; Fried, Michael G (2007). "Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid ... Fried MG (1989) "Measurement of protein-DNA interaction parameters by electrophoresis mobility shift assay". Electrophoresis 10 ...
https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay
*  DSpace at KIST: Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2...
Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2 cardiac myoblast ... Capillary electrophoretic mobility shift assay for binding of DNA with NFAT3, a transcription factor from H9c2 cardiac myoblast ...
http://pubs.kist.re.kr/handle/201004/40652
*  Oncogenic kinase NPM/ALK induces through STAT3 expression of immunosuppressive protein CD274 (PD-L1, B7-H1) | PNAS
Electrophoretic Mobility Shift Assay.. Nuclear proteins were extracted and incubated with biotin-labeled DNA probes, gel- ... we documented STAT3 binding in the gel electromobility shift assay (EMSA) (Fig. 4C). The specificity of binding of both probes ... as shown in the gel electromobility shift and chromatin immunoprecipitation assays, and is required for the PD-L1 gene ... siRNA Assay.. A mixture of four STAT3- or STAT5B-specific siRNA or nontargeting siRNA (all purchased from Dharmacon) was ...
http://www.pnas.org/content/105/52/20852
*  Cyanobacteriochrome CcaS regulates phycoerythrin accumulation in Nostoc punctiforme, a group II chromatic adapter | PNAS
A) Electrophoretic mobility shift assay of CcaR. Purified CcaR [0 ng (lane 1), 100 ng (lane 2), 200 ng (lane 3), or 300 ng ( ... Electrophoretic Mobility Shift Assay.. For probes, the promoter region of cpeC was amplified with the primer set NpccaRD-3/ ... The results of an electrophoretic mobility shift assay suggest that CcaR binds to the promoter region of cpeC-cpcG2-cpeR1, ... examined the ability of CcaR to bind to the promoter region of cpeC-cpcG2-cpeR1 using the electrophoretic mobility shift assay ...
http://www.pnas.org/content/107/19/8854
*  Fatty acids acutely enhance insulin-induced oxidative stress and cause insulin resistance by increasing mitochondrial reactive...
Electrophoretic mobility shift assay. Nuclei from rat muscle were prepared with a modified version of previously described ... The final concentrations in the assay were: carbonyl cyanide m-chlorophenyl hydrazone (CCCP; 5 μmol/l); antimycin A (AA; 1 μg/ ... The major findings of this study are that: (1) hyperinsulinaemia in the high physiological range causes a shift towards a more ... Glutathione is the major buffering system for scavenging ROS, and a shift towards its oxidised form reflects a more oxidised ...
https://link.springer.com/article/10.1007%2Fs00125-011-2396-x
*  Rescue From Oculocutaneous Albinism Type 4 Using Medaka slc45a2 cDNA Driven by Its Own Promoter | Genetics
Electrophoretic mobility shift assay (EMSA) using probes containing the Py-rich motif of mouse (motif D in Figure 5A) and ... Electrophoretic mobility shift assay:. The mouse B16 melanoma cell line and the mouse L929 fibroblast cell line were cultured ... Complementary oligonucleotide probes (see Figure 6A for the sequence of one strand) for electrophoretic mobility shift assay ( ... In a competition assay, 1 or 10 pmol (10-fold or 100-fold molar excess, respectively) of nonradiolabeled probe DNA was added to ...
http://www.genetics.org/content/178/2/761
*  Androgen Regulation of 5α-Reductase Isoenzymes in Prostate Cancer: Implications for Prostate Cancer Prevention
Electrophoretic mobility shift assay Is the Subject Area "Electrophoretic mobility shift assay" applicable to this article? Yes ... Electrophoretic mobility-shift assay (EMSA). AR's binding to double-stranded oligos was assessed in LNCaP cell nuclear extracts ... 6C). Only probe 1 showed a mobility shift (Fig. 6D). To confirm that this mobility shift is specific to AR, we added anti-AR ... probe 1 showed a further mobility shift (Fig. 6D). Although unlabeled wild-type probe 1 was able to compete with labeled probe ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0028840
*  NF-κB Affects Proliferation and Invasiveness of Breast Cancer Cells by Regulating CD44 Expression
Electrophoretic mobility shift assay Is the Subject Area "Electrophoretic mobility shift assay" applicable to this article? Yes ... Electrophoretic mobility shift assays (EMSA) showed decreased NF-κB binding at 2.5 µM of Bay-11-7082 treatment for 72 hrs in ... Electrophoretic mobility shift assay (EMSA). A double stranded DNA probe with the sequence 5′-GATCCGGCAGGGGAATCTCCCTCTC-3′ was ... Electrophoretic mobility shift assays (EMSA) were performed to determine the ability of NF-κB to bind to DNA following ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106966
*  Plus it
Electrophoretic mobility shift assay (EMSA). EMSA experiments were performed as previously described (Valsecchi et al., 2011). ... For LDH assay, cells were plated in 24 well plates at a density of 1 × 106 cells/well; for luciferase assay, they were plated ... for LDH assay) and 15 μg for 100 mm plates (for Western blot analysis) of the above mentioned constructs. For luciferase assay ... Re-ChIP assay revealed that Sp3, REST, HDAC1, and HDAC2 (Fig. 6e,g,h, lanes 4), and Sp1, HIF-1, and p300 (Fig. 6f,i, lanes 5) ...
http://www.jneurosci.org/content/35/19/7332
*  Efferent Vagal Fibre Stimulation Blunts Nuclear Factor-κB Activation and Protects Against Hypovolemic Hemorrhagic Shock |...
Electrophoretic Mobility Shift Assay. NF-κB binding activity was performed as previously reported.6 The binding bands were ... Figure 1. Electrophoretic mobility shift assay of NF-κB in rat liver. Rats underwent hemorrhagic shock (Hem) or sham shock ( ... because the integrated intensity of group samples from different electrophoretic mobility shift assay batches would be affected ...
http://circ.ahajournals.org/content/107/8/1189.long
*  Ubiquitin-Proteasome Pathway as a New Target for the Prevention of Restenosis | Circulation
Electrophoretic Mobility Shift Assay. Nuclear binding of NFκB was analyzed by electrophoretic mobility shift assays, with VSMC ... C, electrophoretic mobility shift assay analysis of MG132 and clasto-lactacystin (lacta)-mediated inhibition of TNFα-induced NF ... degradation of the cytosolic inhibitor proteins IκBα and β by Western blotting and by electrophoretic mobility shift assay ... VSMC Proliferation Assay. Primary VSMCs were seeded onto 24-well plates. Cells were incubated for 5 minutes with MG132 (1, 10, ...
http://circ.ahajournals.org/content/105/4/483
*  Multiple PU.1 sites cooperate in the regulation ofp40 phox transcription during granulocytic differentiation of myeloid cells |...
Electrophoretic mobility shift assay. Complementary DNA oligonucleotides were annealed by heating in 1× NET at 95°C for 5 ... electrophoretic mobility shift assay (EMSA) was carried out using32P-labeled double-stranded oligonucleotides corresponding to ... The affinity of binding of PU.1 to thep40phox PU.1 oligonucleotide probes in the gel mobility shift assay and the transacting ... For gel shift assays, nuclear extract (6 μg) was incubated for 20 minutes at ambient temperature with 5 × 104 cpm of the ...
http://www.bloodjournal.org/content/99/12/4578?ijkey=2efb50f13666aa14ef6b4d8ecd6e8e8b4cfa76b4&keytype2=tf_ipsecsha&sso-checked=true
*  C/EBPα Regulates Human Adiponectin Gene Transcription Through an Intronic Enhancer | Diabetes
Electrophoretic mobility shift assay.. Electrophoretic mobility shift assay (EMSA) was performed using the LightShift ... Gel super-shift analyses using 21-bp oligonucleotides and C/EBPα synthesized in vitro were performed as described in research ... Competition assays were performed using 200-fold excess of unlabeled oligonucleotides (comp ×200). The anti-C/EBPα antibody (Ab ... 5C depict the mean ± SE of at least four independent assays. ★P , 0.05 vs. cells transduced by GFP adenovirus. ...
http://diabetes.diabetesjournals.org/content/54/6/1744.full
*  Cosegregation of MIDD and MODY in a Pedigree | Diabetes
Electrophoretic mobility shift assay.. HeLa cells were transfected with 5 μg of wild-type or mutant HNF1α-pcDNA3.1. After 24 h ... Electrophoretic mobility shift assay demonstrating DNA-binding ability of wild-type and mutated HNF1α to the binding sequence ... and the electrophoretic mobility shift assay was performed (24) using 4.0 μg of nuclear extract with 32P-labeled, GLUT2 ... Transactivation assay.. HeLa cells (1.5 × 105) or MIN6 cells (3 × 105) were transfected using LipofectAMINE Plus Reagent (Life ...
http://diabetes.diabetesjournals.org/content/53/7/1894.long
*  Activator Protein 2α Inhibits Tumorigenicity and Represses Vascular Endothelial Growth Factor Transcription in Prostate Cancer...
Electrophoretic Mobility Shift Assay.. Electrophoretic mobility shift assay for AP-2 was performed as described previously (3) ... Three shifted complexes were observed on the electrophoretic mobility shift assay gel. Addition of an anti-AP-2 antibody did ... We examined next the functionality of the exogenous AP-2 protein by electrophoretic mobility shift assay (Fig. 1C) ⇓ . The AP-2 ... C, electrophoretic mobility shift assay: nuclear extracts were incubated with radiolabeled AP-2 consensus sequence, and the DNA ...
http://cancerres.aacrjournals.org/content/64/2/631?ijkey=4fb8e7ccd20dad7756fb230ecfba87bdf07c41f4&keytype2=tf_ipsecsha