Inhibition of carcinogen induced biological responses with a coffee water-insoluble fraction and a model system melanoidin -...
Isoelectric Focusing Electrophoresis Coffee WIF was analyzed by electrofocusing polyacrylamide gel electro-phoresis with 1% ... High resolution two dimensional electrophoresis of proteins. 3. Biol. Chem. 250:4007. Ong, T.M. 1975. Afiatoxin mutagenesis. ... This cleaning of the glass tubes facilitated the removal of the acrylamide gel after electrophoresis. Each tube was sealed at ... The procedure was similar to that of OFarrell (1975), but only one dimensional electrophoresis was employed. Glass tubes (130 ...
Identification of a novel secretion system in Leishmania : composition, mechanisms, and immune modulating properties - UBC...
Chapter 4: Joachim Clos generated HSP100 null mutant L. donovani and conducted 2D gel electrophoresis and MALDI-TOF-MS. 1 1. ... two dimensional BB: bromophenol blue BFA: brefeldin A CA: cell associated proteins Cm: conditioned media cpm: counts per minute ... 4.6.9 2-D gel electrophoresis and MALDI-TOF mass spectrometry Leishmania, ≈ 1x109, were sedimented (10 min, 690xg, 4°C) and ... 127 4.6.9 2-D gel electrophoresis and MALDI-TOF mass spectrometry ..................................... 127 4.6.10 Quantitative ...
Peptidoglycan-modifying enzymes : mechanistic studies and substrate and inhibitor design - UBC Library Open Collections
saturated SDS-PAGE sodium dodecylsulfate polyacrylamide gel electrophoresis SIS sugar phosphate isomerase/sugar phosphate ... While the overall shape of peptidoglycan isolated from bacteria is maintained, a number of the native three-dimensional ... Silica gel chromatography was performed using Silica Gel SiliaFlash F60 (230-400 mesh, Silicycle). Pyridine, triethylamine, 70 ... Silica gel chromatography was performed using Silica Gel SiliaFlash F60 (230-400 mesh, Silicycle). Pyridine, triethylamine, ...