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*  Cryo-electron tomography of cells: connecting structure and function | SpringerLink
Cryo-electron tomography (cryo-ET) allows the visualization of cellular structures under close-to-life conditions and at ... Mastronarde DN (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152 ... Cryo-electron tomography Correlative light microscopy Electron microscopy Histochemistry and Cell Biology Lecture presented at ... Cryo-electron tomography (cryo-ET) allows the visualization of cellular structures under close-to-life conditions and at ...
https://link.springer.com/article/10.1007%2Fs00418-008-0459-y
*  Electron tomography reveals a flared morphology on growing microtubule ends | Journal of Cell Science
2005). Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36-51. ... Electron tomography. For tomography, the grids were placed in a high tilt holder (Model 2020; Fischione Instruments; Corporate ... Electron tomography reveals a flared morphology on growing microtubule ends Message Subject (Your Name) has sent you a message ... Electron tomography reveals a flared morphology on growing microtubule ends. Johanna L. Höög, Stephen M. Huisman, Zsofia Sebö- ...
http://jcs.biologists.org/content/124/5/693
*  EM3D - Software for Electron Microscope Tomography
EM3D: Software for Electron Microscope Tomography. EM3D is an integrated software application designed to facilitate the ... analysis and visualization of electron microscope (EM) tomography data by cellular and molecular biologists. Such data are ... and create an integrated software package that would take raw electron microscope image data to transform into individual ... a sequence of 2D electron micrographs taken at many tilt angles with respect to the electron beam. ...
http://em3d.stanford.edu/about.html
*  EM3D - Software for Electron Microscope Tomography
Methods for generating high-resolution structural models from electron microscope tomography data. Structure : 12 (10):1763- ... Dual axis reconstruction - Takes advantage of the full capability of high end electron microscopes for tomography. ...
http://em3d.org/release_information.html
*  EM3D - Software for Electron Microscope Tomography
The Tutorial has been updated for EM3D 2.0. Please read Release Information to check for the latest features added to EM3D version 2.0. ...
http://em3d.org/tutorials.html
*  CiteSeerX - Search Results - A Model Based Iterative Reconstruction Algorithm For High Angle Annular Dark Field-Scanning...
A Model Based Iterative Reconstruction Algorithm For High Angle Annular Dark Field-Scanning Transmission Electron Microscope ( ... Transmission Electron Microscope Tomography of Nanostructured Materials by Kent Ridge, Digital Labs, Ming Lin, Hui Ru Tan, ... Using high angle annular dark field scanning TEM tomography, we reveal the interaction between the clay layers and the epoxy ...
https://citeseerx.ist.psu.edu/search?q=A+Model+Based+Iterative+Reconstruction+Algorithm+For+High+Angle+Annular+Dark+Field-Scanning+Transmission+Electron+Microscope+%28HAADF-STEM%29+Tomography
*  Architecture of Thylakoid Membrane Networks | Springer for Research & Development
Cryo-electron microscopy of vitreous sections. EMT. Electron microscope tomography. EM. Electron microscopy ... Penczek P, Marko M, Buttle K and Frank J (1995) Double-tilt electron tomography. Ultramicroscopy 60: 393-410PubMedGoogle ... Baumeister W (2002) Electron tomography: towards visualizing the molecular organization of the cytoplasm. Curr Opin Struc Biol ... Granick S and Porter KR (1947) The structure of the spinach chloroplast as interpreted with the electron microscope. Am J Bot ...
https://rd.springer.com/chapter/10.1007%2F978-90-481-2863-1_14
*  Cryo-electron tomography reveals conserved features of doublet microtubules in flagella | PNAS
2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152:36-51. ... 2009) Cryo-electron microscope tomography to study axonemal organization. Methods Cell Biol 91:1-39. ... Cryo-electron tomography reveals conserved features of doublet microtubules in flagella. Daniela Nicastro, Xiaofeng Fu, Thomas ... Cryo-electron tomography reveals conserved features of doublet microtubules in flagella. Daniela Nicastro, Xiaofeng Fu, Thomas ...
https://www.pnas.org/content/108/42/E845?ijkey=ffd2ecb180f12fb0d59062e0161a2a897c39fc0d&keytype2=tf_ipsecsha
*  Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex | Nature
Automated electron microscope tomography using robust prediction of specimen movements. . J. Struct. Biol. 152, 36-51 (2005) ... Volta potential phase plate for in-focus phase contrast transmission electron microscopy. . Proc. Natl Acad. Sci. USA 111, ... EMAN2: an extensible image processing suite for electron microscopy. . J. Struct. Biol. 157, 38-46 (2007) ... MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. . Nat. Methods 14, 331-332 ( ...
https://www.nature.com/articles/nature25773?error=cookies_not_supported&code=cc46c835-d7c4-4f96-8474-fe25f126396a
*  Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus | PNAS
2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152:36-51. ... 2017) In situ structural analysis of the spirochetal flagellar motor by cryo-electron tomography. Methods Mol Biol 1593:229-242 ... 2007) 3D reconstruction and processing of volumetric data in cryo-electron tomography. J Struct Biol 157:126-137. ... The frozen-hydrated specimens were transferred to a Polara G2 electron microscope (FEI) equipped with a 300-kV field emission ...
http://www.pnas.org/content/114/41/10966
*  Structural and molecular basis for Ebola virus neutralization by protective human antibodies | Science
Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36-51 (2005). doi ... Bsoft: Image processing and molecular modeling for electron microscopy. J. Struct. Biol. 157, 3-18 (2007). doi:10.1016/j.jsb. ... EMAN2: An extensible image processing suite for electron microscopy. J. Struct. Biol. 157, 38-46 (2007). doi:10.1016/j.jsb. ... Accurate determination of local defocus and specimen tilt in electron microscopy. J. Struct. Biol. 142, 334-347 (2003). doi: ...
http://science.sciencemag.org/content/351/6279/1343.long
*  The membrane protein Pannexin1 forms two open-channel conformations depending on the mode of activation | Science Signaling
Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36-51 (2005).. ... Single-particle electron microscopic analysis revealed that K+ stimulated the formation of channels with a larger pore diameter ... Electron microscopic analysis of the pore diameter of Panx1 channels under two conditions reveals stimulus-dependent size ... EMAN2: An extensible image processing suite for electron microscopy. J. Struct. Biol. 157, 38-46 (2007).. ...
http://stke.sciencemag.org/content/7/335/ra69?ijkey=b369ec347d5ff23b3dc432dbc73c20a3c8de9989&keytype2=tf_ipsecsha
*  High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly | SpringerLink
Electron cryo-tomography and subtomogram averaging enable visualization of protein complexes in situ, in three dimensions, in a ... Mastronarde DN (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152 ... An automatic method of detecting and tracking fiducial markers for alignment in electron tomography. J Electron Microsc 60:39- ... To illustrate the electron cryo-tomography workflow for visualizing protein complexes in situ, we describe our workflow of ...
https://link.springer.com/protocol/10.1007%2F978-1-4939-7759-8_2
*  Paramyxovirus Ultrastructure and Genome Packaging: Cryo-Electron Tomography of Sendai Virus | Journal of Virology
Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152:36-51. ... Cryo-electron tomography.Purified SeV was mixed with 10-nm colloidal gold and prepared for electron microscopy by loading 5-μl ... Paramyxovirus morphology.Purified SeV was imaged in the cryo-electron microscope under low-electron dose conditions. Four tilt ... Paramyxovirus Ultrastructure and Genome Packaging: Cryo-Electron Tomography of Sendai Virus. Colin Loney, Geneviève Mottet- ...
https://jvi.asm.org/content/83/16/8191
*  The Nucleus | Springer for Research & Development
Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography Pavel Tchelidze, Hervé Kaplan, ...
https://rd.springer.com/book/10.1007/978-1-59745-406-3
*  Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells |...
2005). Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36-51. doi ... Similar to the electron-dense tethers that have been described by using electron microscopy tomography (Rowland and Voeltz, ... Soft X-ray tomography. The cryogenic soft x-ray microscope XM-2, located at the National Center for X-ray Tomography (http:// ... 2000). Computed tomography of cryogenic biological specimens based on X-ray microscopic images. Ultramicroscopy 84, 185-197. ...
http://jcs.biologists.org/content/128/15/2795
*  Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium "Candidatus Pelagibacter ubique" | Applied and...
Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152:36-51. doi:10.1016/j. ... In situ cryo-electron tomography: a post-reductionist approach to structural biology. J Mol Biol 428:332-343. doi:10.1016/j.jmb ... Electron tomography: towards visualizing the molecular organization of the cytoplasm. Curr Opin Struct Biol 12:679-684. doi: ... New views of cells in 3D: an introduction to electron tomography. Trends Cell Biol 15:43-51. doi:10.1016/j.tcb.2004.11.009. ...
https://aem.asm.org/content/83/3/e02807-16?ijkey=5c416660df2e17d454676b0d9422796b3e2c0b1b&keytype2=tf_ipsecsha
*  Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA Promotes Virus Assembly and Genome Packaging | Journal of Virology
Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152:36-51. doi:10.1016/j. ... TOM software toolbox: acquisition and analysis for electron tomography. J Struct Biol 149:227-234. doi:10.1016/j.jsb.2004.10. ... Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proc Natl Acad Sci U S A 102:4729- ... Determination of protein structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging. J Struct Biol ...
https://jvi.asm.org/content/90/9/4593
*  Structural basis for assembly and function of the Nup82 complex in the nuclear pore scaffold | JCB
Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152:36-51. doi:10.1016 ... Electron tomography revealed that the Nup82 complex consists of a 20-nm Dyn2-DIDNup159 stalk, from which an asymmetric head ... 3D electron tomography structure of the Nup82 complex. Because the asymmetric structure of the Nup82 complex is quite ... Based on the yeast 3D model, we specify the orthologous Nup88-Nup214 complex in the electron tomography map of the human NPC. ...
http://jcb.rupress.org/content/208/3/283
*  Henri A. Levy - Wikipedia
"Electron microscope tomography: Transcription in three dimensions". Science. 220 (4596): 498-500. doi:10.1126/science.6836293. ... In his later life, Levy worked on electron tomography of large biological complexes, particularly those transcribing DNA. Levy ...
https://en.wikipedia.org/wiki/Henri_A._Levy
*  Modes of flagellar assembly in Chlamydomonas reinhardtii and Trypanosoma brucei | eLife
Comparative electron tomography of growing flagellar tips reveals that axonemal growth differs between short and long flagella ... Automated electron microscope tomography using robust prediction of specimen movements * DN Mastronarde ... Using a form of electron microscopy called electron tomography, Höög et al. could see the detailed structure of the growing ... In cryo-electron tomography of an intact T. brucei old flagellum tip, we see clear electron densities in the distal end of dMT ...
https://elifesciences.org/articles/01479
*  Structural basis for anion conduction in the calcium-activated chloride channel TMEM16A | eLife
Automated Electron microscope tomography using robust prediction of specimen movements * DN Mastronarde ... The electron density map has been deposited in the Electron Microscopy Data Bank under the accession code EMD-3658 and the ... Images of flash-frozen samples were recorded on a FEI TITAN Krios electron microscope equipped with an energy filter and a K2- ... Cryo-EM data were collected on a 300 kV FEI Titan Krios electron microscope using a post-column quantum energy filter (Gatan) ...
https://elifesciences.org/articles/26232
*  Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1, Nature...
Automated electron microscope tomography using robust prediction of specimen movements. Mastronarde, DN ... SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields ... EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy ...
https://www.deepdyve.com/lp/springer_journal/epitope-based-vaccine-design-yields-fusion-peptide-directed-antibodies-T0cbuB6Jor
*  Images of synaptic vesicle tethers. (A-D) Short (A an | Open-i
Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography. Fernández-Busnadiego R, Zuber B, Maurer ... The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic ... The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic ... Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography. Fernández-Busnadiego R, Zuber B, Maurer ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2812849_JCB_200908082_GS_Fig5&req=4
*  Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography | Protocol (Translated to Hindi)
Transmission electron microscope. FEI or Jeol. 300 keV. Direct electron detector. Gatan. K2 summit. 4k x 4k pixels, counted ... Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. Journal of ... Maimon, T., Elad, N., Dahan, I., Medalia, O. The human nuclear pore complex as revealed by cryo-electron tomography. Structure ... Bharat, T. A., et al. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. ...
https://www.jove.com/video/51228/-mitochondria-synthase-dimers-?language=Hindi