Loading...
*  The number and location of EF hand motifs dictates the calcium dependence of polycystin-2 function. | Sigma-Aldrich
... both EF hands bind calcium. To test whether the EF hand motif is truly a calcium sensor controlling PC2 channel function, we ... The C-terminal tail of hPC2 contains 2 EF hand motifs, but only the second binds calcium. Here, we investigate whether these EF ... calcium-binding EF hand is intact. These results suggest that the number and location of calcium-binding sites in the EF hand ... The number and location of EF hand motifs dictates the calcium dependence of polycystin-2 function.. [Ivana Y Kuo, Camille ...
https://www.sigmaaldrich.com/catalog/papers/24558196
*  Metalloprotein - Wikipedia
It is a small protein that contains four EF-hand motifs, each of which is able to bind a Ca2+ ion. ... In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. Six glutamic acid and aspartic ... Freisinger EF, Vasac M (2013). "Chapter 11. Cadmium in Metallothioneins". In Sigel A, Sigel H, Sigel RK. Cadmium: From ... Iron(II), on the other hand, can easily be oxidized to iron(III). This functionality is used in cytochromes, which function as ...
https://en.wikipedia.org/wiki/Metalloprotein
*  Actin Dynamics Associated with Focal Adhesions
EF, EF hand motif; EVH1, Ena/VASP homology domain 1; EVH2, Ena/VASP homology domain 2. ... The direct interaction of the EVH1 domain with the FPPPP motifs of zyxin directs the localization of Ena/VASP proteins to FAs [ ... The FH1 domain contains several proline-rich motifs that interact with profilin. The FH2 domain interacts with actin and ... Finally the C-terminal end contains a tetramerization motif (Figure 2). ...
https://www.hindawi.com/journals/ijcb/2012/941292/
*  KAKEN - Research Projects | REGULATION AND INTRACELLULAR LOCALISATION OF PHOSPHOIN OSITIDE-BINDING PROTEINS. (KAKENHI-PROJECT...
Publications] Yamamoto,T.: 'Involvement of EF hand motifs in the Ca^,2+,-dependent binding of the pleckstrin homology domain to ... Publications] Yamamoto T.: 'Involvement of EF hand motifs in the Ca^,2+,-dependent binding of the pleckstrin homology domain to ... Involvement of EF hand motifs in the Ca^,2+,-dependent binding of the pleckstrin homology domain to phosphoinositides.'Eur.J. ...
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10490023/
*  Treesearch - scientific articles written or published by the U.S. Forest Service
JnCML-like, an EF-hand motif-containing gene seasonally upregulated in the transition zone of black walnut (Juglans nigra L.). ...
https://www.fs.usda.gov/treesearch/search?keywords=%22Juglans+nigra%22
*  RCSB PDB - Protein Feature View - Respiratory burst oxidase homolog protein B - Q5ZAJ0 (RBOHB ORYSJ)
Monomer and homodimer, stabilized by swapping the EF-hand motifs. Interacts with GTP-bound RAC1. UniProt ...
http://www.rcsb.org/pdb/protein/Q5ZAJ0
*  Calcium-Binding Proteins Summary Report | CureHunter
They typically contain EF HAND MOTIFS.. Also Known As: Calcium Binding Proteins; Binding Proteins, Calcium ... 08/01/2012 - "Presence of an EF hand domain, a characteristic feature of calcium binding proteins and a role in the synthesis ...
http://www.curehunter.com/public/keywordSummaryD002135-Calcium-Binding-Proteins.do
*  Ca2+-Activated Reactive Oxygen Species Production by Arabidopsis RbohH and RbohJ Is Essential for Proper Pollen Tube Tip Growth...
Two EF-hand motifs are indicated. The Ca2+ binding loops in the first and second EF-hand motifs are boxed. The consensus Ca2+ ... Ca2+ Activates the ROS-Producing Activity of RbohH and RbohJ via the EF-Hand Motif. ... A) and (B) Complementation analysis of the rbohH-3 rbohJ-2 double mutant phenotype with GFP-tagged wild-type or EF-hand mutants ... C) Subcellular localization of GFP-tagged wild-type and EF-hand mutants (E219Q and E229Q) in pollen tubes of the rbohH-3 rbohJ- ...
http://www.plantcell.org/content/26/3/1069/tab-figures-data
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
CsCaM protein comprises four calcium-binding EF-hand motifs. The amino acid sequence of CsCaM shares very high homology with ... Here, a multilocus analysis based on eight genes (ITS1, act, tub, ef-1a, cox1, cox3, nad4 and nad5 [4.986 kb]) was employed to ... A thioredoxin domain was found in the position of aa21-117 and contained the putative active-site motif Cys-Pro-Ala-Cys. BLASTx ... binding sites or KGD motif with the capability of Ca(2+)-binding. In addition, immunoblot assay revealed that recombinant ...
https://www.jove.com/visualize?author=Chi%20Liang%20
*  TCDB » SEARCH
Possesses two tandem Ca2+-binding EF-hand motifs, and cytosolic free Ca2+ (~300nM) activates (Czempinski et al., 1997). ...
http://www.tcdb.org/search/result.php?tc=1.A.1.7.3
*  Calcium binding protein - RightDiagnosis.com
They typically contain EF HAND MOTIFS. Source: MeSH 2007 Calcium binding protein: Related Topics. These medical condition or ...
http://www.rightdiagnosis.com/medical/calcium_binding_protein.htm
*  Actn1 actinin, alpha 1 [Mus musculus (house mouse)] - Gene - NCBI
Ca2+ binding induces a conformational change in the EF-hand motif, leading to .... cd00176. Location:395 → 622. SPEC; Spectrin ... EFhand_Ca_insen; Ca2+ insensitive EF hand. pfam13499. Location:752 → 816. EF-hand_7; EF-hand domain pair. ... EFhand_Ca_insen; Ca2+ insensitive EF hand. pfam13499. Location:752 → 838. EF-hand_7; EF-hand domain pair. ... EFhand_Ca_insen; Ca2+ insensitive EF hand. pfam13499. Location:752 → 811. EF-hand_7; EF-hand domain pair. ...
https://www.ncbi.nlm.nih.gov/gene/?term=109711
*  EFCAB6 EF-hand calcium binding domain 6 [Homo sapiens (human)] - Gene - NCBI
EF-hand_7; EF-hand domain pair. cl08302. Location:408 → 468. EFh; EF-hand, calcium binding motif; A diverse superfamily of ... EF-hand_11; EF-hand domain. pfam13499. Location:71 → 129. EF-hand_7; EF-hand domain pair. cl08302. Location:302 → 362. EFh; EF- ... EF-hand_11; EF-hand domain. pfam13499. Location:19 → 77. EF-hand_7; EF-hand domain pair. cl08302. Location:250 → 310. EFh; EF- ... EF-hand_11; EF-hand domain. pfam13499. Location:71 → 129. EF-hand_7; EF-hand domain pair. cl08302. Location:302 → 362. EFh; EF- ...
https://www.ncbi.nlm.nih.gov/gene/64800
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
It was identified as an alpha (?)-actinin containing a CaM-like domain with EF-hand motifs. Der f 24 reacted to sera from 85.4 ... Two HMG-binding motifs were detected in this region. Mutation of these motifs prevented EGFP expression. Furthermore, EGFP ... The KGD motif in vasotab TY may facilitate the binding of it to GPIIb/IIIa. Vasotab TY showed a half-life of more than 1h in ... On the other hand, if the goal is to use these bound proteins as a linker group to build more complex structures, such as when ...
https://www.jove.com/visualize?author=Ren-Hau+Lai
*  Anti-EFHC1 antibody (ab80861) | Abcam
EFHC1 gene encodes a protein with an EF-hand motif; EFHC1 protein affects programmed cell death through effects on calcium ...
https://www.abcam.com/efhc1-antibody-ab80861.html
*  Mitochondrial Rho
Miro has two EF hand motifs that bind calcium; thus, Miro can couple calcium increase with reduced mitochondrial motility to ... Genetic studies further indicate that both GTPase domains and both EF-hand motifs are required for Gem1p function (Guo, 2005). ... Genetic studies indicate both GTPase domains and EF-hand motifs, which are exposed to the cytoplasm, are required for Gem1p ... which contain two GTPase domains flanking a pair of calcium-binding EF-hand motifs. Gem1p (yeast Miro; encoded by YAL048C) is a ...
http://www.sdbonline.org/sites/fly/sturtevant/miro1.htm
*  A Rice Ca2+ Binding Protein Is Required for Tapetum Function and Pollen Formation | Plant Physiology
A, 3D structure of EF-hand motif of YesW. B, 3D structure of EF-hand motif of OsDEX1. C, In vitro Ca2+ binding assay shows that ... and usually contain EF-hand motifs that are rich in negative amino acid for the coordination with Ca2+. EF-hand usually form a ... EF)-hand domains for Ca2+ binding (Snedden and Fromm, 1998). The typical EF-hand is a helix-loop-helix structure, in which the ... The cDNA of mutated EF-hand motif 2 was amplified by the primers mEF2 F1 and mEF2 R1, and mEF2 F2 and mEF2 R2. The PCR products ...
http://www.plantphysiol.org/content/172/3/1772.full
*  Crosstalk Between Voltage-Independent Ca2+ Channels and L-Type Ca2+ Channels in A7r5 Vascular Smooth Muscle Cells at Elevated...
In CaMQ, all four EF-hand motifs are mutated.16,17 The N-terminally tagged YFP-CaM(Q) was prepared by 3-piece ligation. For ...
http://circres.ahajournals.org/content/92/8/888
*  An EF-hand mutant of CRACR2A causes spontaneous cluster | Open-i
a) CRACR2A binds Ca2+ via its EF-hands. 45Ca2+ overlay experiments were performed with pur ... An EF-hand mutant of CRACR2A causes spontaneous clustering of STIM1. ( ... 2 suggest that the EF-hand motifs of CRACR2A bind to Ca2+ and Ca2+ binding reduces its interaction with Orai1 and STIM1. ... Figure 5: An EF-hand mutant of CRACR2A causes spontaneous clustering of STIM1. (a) CRACR2A binds Ca2+ via its EF-hands. 45Ca2+ ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2875865_nihms191435f5&req=4
*  Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15 and contributes to normal...
In this case, Ca2+ ions are bound only to the N-terminal EF-hands while the C-terminal EF motifs mediate tethering to the ... CaMs contain two pairs of Ca2+ binding EF-hand motifs joined by a linker domain. Upon Ca2+ binding, the shape of the CaM ... Day IS, Reddy VS, Ali GS, Reddy ASN (2002) Analysis of EF-hand-containing proteins in Arabidopsis. Genome Biol 3(10): ... This part of AtDEG15 exhibits a classical CaM-binding motif (Calmodulin target database) and the motif is highly conserved ...
https://link.springer.com/article/10.1007%2Fs11103-013-0112-6
*  Frontiers | NADPH Oxidase-Dependent Superoxide Production in Plant Reproductive Tissues | Plant Science
... the remaining two hands being EF-type motifs not predicted from the sequence (Oda et al., 2010). Ca2+ binding to EF-hand motifs ... the binding of Ca2+ to EF-hand motifs induces a conformational change that enables the EF-hands to interact with the C-terminus ... It is sufficient for Ca2+ to bind to only one of the EF hands in order to produce a conformational change in the EF-hand region ... As with NOX5- and DUOX-type NOX proteins in animals, Rboh proteins display an N-terminal extension with EF-hand motifs (Bedard ...
https://www.frontiersin.org/articles/10.3389/fpls.2016.00359/full
*  Immunoelectron microscopy reveals colocalization of myo | Open-i
... which tethers together the COOH-terminal Src homology 3 domains of nebulin and nebulette with the EF hand motifs of alpha- ... which tethers together the COOH-terminal Src homology 3 domains of nebulin and nebulette with the EF hand motifs of alpha- ...
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2169455_JCB0012108.f8&req=4
*  Short Stop provides an essential link between F-actin and microtubules during axon extension | Development
We have shown that Shot proteins contain an essential EF-hand motif. Removing the EF-hand does not appear to prevent Shot ... To test whether the EF-hand motifs in Shot L are required for Shot activity, we investigated whether Shot L(A)-ΔEF-hand-GFP, ... ABD, actin-binding domain; EF, EF-hand; GAS2, GAS2 homology motif. (B) The structures of the four Shot N-terminal domains (Lee ... 4000 amino acids and a C-terminal domain that contains EF-hand Ca2+-binding motifs, the GAS2 motif and other conserved ...
http://dev.biologists.org/content/129/5/1195
*  Rasgrp1 RAS guanyl releasing protein 1 [Mus musculus (house mouse)] - Gene - NCBI
Ca2+ binding induces a conformational change in the EF-hand motif, leading to .... pfam00130. Location:542 → 591. C1_1; Phorbol ... RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif. cd00051. Location:474 → 521. EFh; EF-hand, ... most examples in this alignment model have 2 active canonical EF hands. ... calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; ...
https://www.ncbi.nlm.nih.gov/gene?amp=&cmd=Retrieve&dopt=Graphics&list_uids=19419
*  The S100B/RAGE Axis in Alzheimer's Disease
binding helix-loop-helix EF-hand motifs," Biochemical Journal, vol. 405, no. 2, pp. 199-221, 2007. View at Publisher · View at ... J. Krebs and C. W. Heizmann, "Calcium-binding proteins and the EF-hand principle," in Calcium: A Matter of Life or Death, J. ...
https://www.hindawi.com/journals/cpn/2010/539581/ref/