Fibronectin is necessary for embryogenesis, and inactivating the gene for fibronectin results in early embryonic lethality. ... Fibronectin is important for guiding cell attachment and migration during embryonic development. In mammalian development, the ... Fibronectin is located in the extracellular matrix of embryonic and adult tissues (not in the basement membranes of the adult ... "Fibronectin stimulates non-small cell lung carcinoma cell growth through activation of Akt/mammalian target of rapamycin/S6 ...

... has not been established due to the early embryonic lethality of mice with a targeted deletion of the Sin1 gene. To circumvent ... The mammalian target of rapamycin complex 2 (mTORC2) has been implicated in the regulation of cell survival by phosphorylating ...

Essential requirement of mammalian Pumilio family in embryonic development. Lin, Kaibo; Zhang, Shikun; Shi, Qinghua; Zhu, ... Here we report an essential requirement of the Pum gene family in early embryonic development. A loss of both Pum1 and Pum2 ... genes led to gastrulation failure, resulting in embryo lethality at E8.5. Pum-deficient blastocysts, however, appeared ... However, their roles in mammalian embryonic development and stem cell maintenance remained largely uncharacterized. ...

Strikingly, Trim71R595H/R595H mice exhibit anencephaly and embryonic lethality. These results define a single conserved residue ... of the prenatal mammalian brain. Trim71 deletion in prenatal mouse NSCs (Nestin-Trim71fl/fl and Emx-Trim71fl/fl), or knock-in ... in TRIM71 that is indispensable for mammalian brain morphogenesis and shed novel insight into the pathogenesis of the most ... I now show that TRIM71 is a novel marker of neuroepithelial cells, the earliest neural stem cells (NSCs) ...

Simultaneous elimination of both leads to gastrulation failure and early embryonic lethality around E6.5. Embryonic stem cells ... role of MLL3/4 methyltransferase activities in mammalian embryonic development and ESC differentiation. ... MLL3/MLL4 methyltransferase activities control early embryonic development and ESC differentiation in a lineage-selective ... we report that enzymatic activities of MLL3 and MLL4 are redundant during early embryonic development. ...

... results in embryonic lethality and/or larval lethality associated with developmental arrest during molting (Grant and Hirsh, ... Early to late endosome transport may be mediated by small vesicular intermediates, or may be a maturation process whereby early ... Unlike mammalian cells, invertebrates such as C. elegans have many small "mini stacks" throughout the cytoplasm of most cells ... Zygotic embryonic lethality during elongation was found in sec-23 homozygous mutants derived from heterozygous mothers. Normal ...

... and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic ... We show that the conditional depletion of the m6A reader protein Ythdf2 in mice causes lethality at late embryonic ... and targeting of the gene in mouse causes early embryonic lethality [16,17,18,19,20]. The most recent studies in hematopoietic ... This was shown early for the writers of m6A in model organisms [12] and also for the mammalian m6A eraser Alkbh5 [6] and the m6 ...

Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene ... This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements ... PHENOTYPE: Heterozygous mice with a paternally inherited null allele display embryonic lethality during organogenesis with ... It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, ...

Uehara Y, Minowa O, Mori C, Shiota K, Kuno J, Noda T and Kitamura N: Placental defect and embryonic lethality in mice lacking ... At the early stages of development, HGF and Met exhibit expression in the mesoderm and endoderm, respectively, and may act in ... The indispensable roles of the HGF-Met system in mammalian development have been elucidated by the targeted disruption of the ... Andermarcher E, Surani MA and Gherardi E: Co-expression of the HGF/SF and c-met genes during early mouse embryogenesis precedes ...

1)lilli mutations enhance dpp heldout phenotypes and embryonic recessive lethality. The enhancement of dpp embryonic lethality ... during the early stages of germ-band extension. The earlier requirement for Lilli strongly suggests it contributes to the early ... Therefore, it is believed that, like its mammalian homologs, Lilli also normally functions in the nucleus (Tang, 2001). EFFECTS ... Four lilli alleles were tested for dominant maternal enhancement of dpp recessive embryonic lethality. Df(2L)JS17 was excluded ...

We have previously described severe embryonic skeletal abnormalities and perinatal lethality in mice carrying a loss-of- ... specialized care through early and rapid identification and management of critical patterns in wound bioburden. INTRODUCTION ... Distinct expression domains for CS and CS biosynthesis enzymes have been described in the developing and mature mammalian heart ... among multiple subtypes of NSCLC and early stage disease but exhibited only limited efficacy for the discrimination of NSCLC ...

Our study provides multiple genetic inroads into the molecular mechanisms that control early mammalian development and the ... embryonic lethal mutations that can be used to assign phenotypes to developmental milestones and outline the time of lethality. ... Embryonic Structures. Female Urogenital Diseases and Pregnancy Complications. Genetics and Genomics. Male Urogenital Diseases ... The period of development between the zygote and embryonic day 9.5 in mice includes multiple developmental milestones essential ...

... by antisense morpholino and/or transgenic expression of miR-sponge RNA induced significant impairment of both embryonic and ... Disruption of the microRNA 137 primary transcript results in early embryonic lethality in mice. Biol Psychiatry 2015; 77: e5-e7 ... Ebert MS, Neilson JR, Sharp PA . MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods 2007; 4 ... in view of the recent observation of embryonic lethality in the miR-137 homozygous knockout mouse53 it is possible that this ...

For a reverse experiment, DNA methylation in early Xenopus embryos was assessed by MBD affinity capture. A methylated DNA ... The two enrichment strategies allow probing of methyl-CpG protein interactions in early vertebrate oocytes and embryos. ... Li E, Bestor TH, Jaenisch R: Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 1992, 69 ... Hendrich B, Bird A: Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. ...

These results thus showed that Tmem70 knockout in the mouse leads to embryonic lethality due to the lack of ATP synthase and ... Affected embryos displayed 80% decrease of ATP synthase and impaired biogenesis of ATP synthase, stalled at the early stage, ... a 21-kDa protein that facilitates the biogenesis of mammalian enzyme. TMEM70 gene mutations turned out to represent the most ... Tmem70 ablation resulted in profound growth retardation and embryonic lethality at 9.5 days of fetal development. ...

... deficiency of the Prkci isozyme results in early embryonic lethality at embryonic day (E)9.5 (Seidl et al., 2013; Soloff et al ... In the mammalian organs, zinc is mainly concentrated in the brain at around 150 μm. However, free zinc in the mammalian brain ... Here, we investigate the requirement of Prkci in mouse cells using an in vitro system that bypasses early embryonic lethality. ... Embryonic stem (ES) cells are used to make embryoid bodies (EBs) that develop like the early post-implantation embryo in terms ...

Disruption of Trrap causes early embryonic lethality and defects in cell cycle progression. Nature Genetics, 29: 206-211 ... Gospodinov A, Vaissiere T, Krastev D, Legube G, Anachkova B and Herceg Z. (2011) Mammalian Ino80 Mediates Double Strand Break ... Early epigenetic changes ("drivers") in cancer development and in cancer stem cells. Z. Herceg, H. Hernandez-Vargas, M. Martin ... Role of early-life exposures on epigenome and cancer susceptibility in childhood and adulthood. H. Hernandez-Vargas, Z. Herceg ...

In mice, loss of H3.3 genes results in developmental retardation and early embryonic lethality [80]. Although H3.3 is not ... In mammalian cells, H3.3 has been reported to be enriched in highly expressed genes [6, 7]. To examine this possibility, we ... Mammalian H3.1 and H3.2 are deposited by the heterotrimeric chromatin assembly complex 1 (CAF1), whereas H3.3 is deposited at ... An unusual histone H3 specific for early macronuclear development in Euplotes crassus. Proc Natl Acad Sci USA. 1997;94:1332. ...

... mice lacking of the regulatory subunit CK2β displays the early embryonic lethality in a cell autonomous fashion [21]. The ... ProTAME Arrest in Mammalian Oocytes and Embryos Does Not Require Spindle Assembly Checkpoint Activity. Int J Mol Sci. 2019;20( ... of the regulatory beta subunit of protein kinase CK2 in mice leads to a cell-autonomous defect and early embryonic lethality. ... Inhibition of casein kinase 2 blocks G2/M transition in early embryo mitosis but not in oocyte meiosis in mouse. J Reprod Dev. ...

Consequently, loss of EHMT1 in oogenesis results, upon fertilization, in mid-gestation embryonic lethality. To identify H3K9 ... During pre-implantation stages of mammalian development, maternally stored material promotes both the erasure of the sperm and ... By comparing these signatures with early embryos that have undergone spontaneous cleavage-stage arrest, as determined by time- ... We fully characterize embryonic genome activation and maternal transcript degradation and map key epigenetic reprogramming ...

... synchrony of the second embryonic division and embryonic lethality [9],[10]. Dlkb1, another homologue of LKB1 in Drosophila ... Joensuu K, Leidenius M, Kero M, Andersson LC, Horwitz KB, Heikkila P: ER, PR, HER2, Ki-67 and CK5 in early and late relapsing ... LKB1 also plays a critical role in maintenance of mammalian epithelial cell polarity. Research has shown that LKB1 can promote ... Par4, a homologue of human LKB1, has been found to control Caenorhabditis elegans embryonic polarity by regulating activities ...

Bosl, M.R.; Takaku, K.; Oshima, M.; Nishimura, S.; Taketo, M.M. Early embryonic lethality caused by targeted disruption of the ... Su, D.; Novoselov, S.V.; Sun, Q.A.; Moustafa, M.E.; Zhou, Y.; Oko, R.; Hatfield, D.L.; Gladyshev, V.N. Mammalian selenoprotein ... and early embryonic lethality in homozygous mice. Mol. Cell. Biol. 2003, 23, 916-922. [Google Scholar] [CrossRef] [PubMed] ... Embryonic lethality arising from deletion of the trsp gene encoding the Sec-tRNA required for translation [54] demonstrates the ...

As constitutive knockout of Lkb1 in mice leads to embryonic lethality, whether Lkb1 is required for the growth and survival of ... As constitutive knockout of Lkb1 in mice leads to embryonic lethality, whether Lkb1 is required for the growth and survival of ... At the molecular level, Lkb1 limits satellite cell proliferation through the canonical AMP-activated protein kinase/mammalian ... mice bypasses the developmental defects and early death, Lkb1 null satellite cells lose their regenerative capacity cell- ...

Using WISH during zebrafish embryogenesis, we demonstrated that vps50 gene was expressed throughout the early embryo, ... as demonstrated by the embryonic lethality of mice with homozygous null mutations in this pathway, showing abnormalities in the ... We chose the following zebrafish early stages individually: 0.5 dpf, 1 dpf, 2 dpf, and 3 dpf. The early stages tested with the ... Pangarkar C, Dinh AT, Mitragotri S. Dynamics and spatial organization of endosomes in mammalian cells. Phys Rev Lett. 2005;95: ...

Embryonic lethality and vascular defects in mice lacking the Notch ligand Jagged1. Hum Mol Genet. 1999;8(5):723-730.. View this ... Jag1-null mice have been reported to die from hemorrhage early during embryogenesis, exhibiting defects in the embryonic and ... Mammalian Notch genes are widely expressed during embryonic development, suggesting that Notch regulates the differentiation of ... Stages of embryonic development of the zebrafish. Dev Dyn. 1995;203(3):253-310.. View this article via: CrossRef PubMed Google ...

Human BRCA1 gene rescues the embryonic lethality of Brca1 mutant mice. Genesis. 29:72-77. View this article via: CrossRef ... BRCA1-null mice die early in embryonic development. Ostensibly, human BRCA1 BAC constructs contain the appropriate regulatory ... do not measure physiologic BRCA1 activity within the context of a mammalian cell. Data overwhelmingly link the tumor ... Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2 [see comments]. Nature. 386:804-810 ...

65] have observed that deficiency of both Nrf1 and Nrf2 results in early embryonic lethality of mice and this indicates a ... This process is regulated by SKN-1 by enhancing DCT-1 expression, a C. elegans homolog of mammalian BNIP3 and BNIP3L/NIX, key ... Deficiency of the Nrf1 and Nrf2 transcription factors results in early embryonic lethality and severe oxidative stress. J Biol ... It is well established that HO-1-deficiency results in embryonic death and some studies evaluated the role of HO-1 in embryonic ...

2004) Disruption of the mouse mTOR gene leads to early postimplantation lethality and prohibits embryonic stem cell development ... Cao R, Li A, Cho HY, Lee B, Obrietan K (2010) Mammalian target of rapamycin signaling modulates photic entrainment of the ... 2004) mTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells. Mol Cell Biol 24:6710- ... Mammalian/mechanistic target of rapamycin (mTOR) signaling controls cell growth, proliferation, and metabolism in dividing ...

Neonatal lethality in CPEB2-KO mice first revealed the regulatory role of CPEB2 in respiration. Although specific ablation of ... Long-term failure of alveologenesis after an early short-term exposure to a PDGF-receptor antagonist. Am J Phys Lung Cell Mol ... Lung organogenesis in both humans and mice proceeds through 5 morphogenesis phases- embryonic, pseudoglandular, canalicular, ... including the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin pathway [12] and mitogen-activated protein kinase ...

Deficiency of the Nrf1 and Nrf2 transcription factors results in early embryonic lethality and severe oxidative stress. J Biol ... Tocotrienols regulate cholesterol production in mammalian cells by post-transcriptional suppression of 3-hydroxy-3- ...