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*  Human Topoisomerase II Assay Kit, Topo II Assay, kDNA, Topoisomerase II, Measure Topo II Activity in a Crude Extract
... measure topo II activity in a crude extract. Highly specific and quantifiable. ... Human Topoisomerase II Assay Kit available from TopoGEN - ... and specific assays for eukaryotic type II DNA topoisomerases. ... DNA substrates and DNA markers are included. Purified topo II is not included in this kit (TG2000H); however, topoisomerase II ... The assay is based upon decatentation of kinetoplast DNA (kDNA) and because it is specific for type II activity (not type I), ...
https://www.topogen.com/topo-assay-kits-drug-screening-and-ice-assay-kits/topoisomerase-assay-kits/human-topoisomerase-ii-assay-kit.html
*  sbmC - DNA gyrase inhibitor - Escherichia coli (strain K12) - sbmC gene & protein
Acts by inhibiting DNA gyrase at an early step, prior to (or at the step of) binding of DNA by the gyrase. It protects cells ... Can also protect cells against alkylating agents that act independently of DNA gyrase, suggesting a more general role in ... against toxins that target DNA gyrase, by inhibiting activity of these toxins and reducing the formation of lethal double- ... DNA topoisomerase (ATP-hydrolyzing) inhibitor activity Source: CACAO ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a ...
http://www.uniprot.org/uniprot/P33012
*  Functional compatibility between isoform α and β of type II DNA topoisomerase | Journal of Cell Science
DNA topoisomerase II (topo II) plays a crucial role in controlling the conformation of both DNA and whole chromosomes. This ... DNA topoisomerase II assay. Extracts for topo II assay were prepared from 4×105 cells 3 days after transfection with siRNAs, as ... DNA topoisomerase II (topo II) is a ubiquitous nuclear enzyme that plays a crucial role in controlling the conformation of both ... Functional compatibility between isoform α and β of type II DNA topoisomerase ...
http://jcs.biologists.org/content/117/7/1047
*  Synthesis and biological activity evaluation of 1H-benzimidazoles via mammalian DNA topoisomerase I and cytostaticity assays |...
The characterized compounds were screened to identify if they interfere with mammalian type I DNA topoisomerase activity via in ... Synthesis and biological activity evaluation of 1H-benzimidazoles via mammalian DNA topoisomerase I and cytostaticity assays. ... Synthesis and biological activity evaluation of 1H-benzimidazoles via mammalian DNA topoisomerase I and cytostaticity assays ... Some benzimidazole derivatives also interfere with the reactions of DNA topoisomerases, the enzymes functioning at almost all ...
http://clincancerres.aacrjournals.org/content/14/19_Supplement/A16
*  PROSITE
... is one of the two types of enzyme that catalyze the interconversion of topological DNA isomers. Type I topoisomerases act by ... have two type I topoisomerase isozymes: topoisomerase I (gene topA) and topoisomerase III (gene topB). Eukaroytes also contain ... When a prokaryotic type I topoisomerase breaks a DNA backbone bond, it simultaneously forms a protein-DNA link where the ... TOPOISOMERASE_I_PROK, PS00396; Prokaryotic DNA topoisomerase I active site (PATTERN). * Consensus pattern:. [EQ]-x-L-Y-[DEQSTLM ...
http://prosite.expasy.org/PDOC00333
*  Research Highlight - Programmed activation of cancer cell apoptosis: A tumor-targeted phototherapeutic topoisomerase I inhibitor
Two p-type conjugated polymers with disparate optical and electronic properties, PB3T and PB2T, were developed and applied in ... Topoisomerase I is a vital enzyme that controls DNA topology during replication, transcription, and recombination. An elevated ... PT-1 interferes with DNA re-ligation, diminishes the expression of topoisomerase I, and enhances the expression of inter alia ... Effect of ion-ligand binding on ion pairing dynamics studied by two-dimensional infrared spectroscopy. Sungnam Park ...
http://chem.korea.ac.kr/index.php?mid=Research_Highlight&page=2&document_srl=2470
*  Intramolecular synapsis of duplex DNA by vaccinia topoisomerase | The EMBO Journal
... as there is in the case of type II topoisomerases.. We analyzed the appearance of topoisomerase-DNA complexes formed at ... type I topoisomerase. Introduction. The type IB DNA topoisomerase family includes eukaryotic topoisomerase I, a ubiquitous ... Electron microscopy of topoisomerase-DNA complexes. (A) Linear pBluescript plasmid DNA. (B) Topoisomerase-DNA complexes formed ... type topoisomerase (Figure 5). Eight or more superhelical turns were introduced per DNA molecule at a topoisomerase:DNA ratio ...
http://emboj.embopress.org/content/16/21/6584.print
*  Teniposide - DrugBank
This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA ... Teniposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. ... to the inhibition of type II topoisomerase activity since teniposide does not intercalate into DNA or bind strongly to DNA. ... Teniposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double ...
https://www.drugbank.ca/drugs/DB00444
*  RCSB PDB - Protein Feature View - Type 2 DNA topoisomerase 6 subunit A - Q57815 (TOP6A METJA)
Homodimer (PubMed:10545127). Heterotetramer of two Top6A and two Top6B chains. UniProt ... However, besides these two, there are many other icons. For more information about the meaning and exact position of a sequence ... RCSB PDB (citation) is managed by two members of the Research Collaboratory for Structural Bioinformatics: Rutgers and UCSD/ ...
http://www.rcsb.org/pdb/protein/Q57815
*  Tightening of DNA knots by supercoiling facilitates their unknotting by type II DNA topoisomerases - Infoscience
... substrates of type IIA topoisomerases because various earlier experiments have concluded that type IIA DNA topoisomerases ... here shows that torsional tension in DNA may serve to expose DNA knots to the unknotting action of type IIA topoisomerases, and ... We observe that DNA supercoiling tightens knotted portions of DNA molecules and accentuates the difference in curvature between ... thus explains how these topoisomerases could maintain a low knotting equilibrium in vivo, even for long DNA molecules. Witz, G ...
https://infoscience.epfl.ch/record/167096
*  DNA topoisomerase IV, subunit B, Gram-negative (IPR005737) | InterPro | EMBL-EBI
Phylogenomics of type II DNA topoisomerases.. Bioessays 25 232-42 2003. Watt PM, Hickson ID. Structure and function of type II ... topoisomerases I, III and V) break single-strand DNA, and type II enzymes (EC:5.99.1.3; topoisomerases II, IV and VI) break ... type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for ... GO:0003677 DNA binding GO:0003918 DNA topoisomerase type II (ATP-hydrolyzing) activity ...
http://www.ebi.ac.uk/interpro/entry/IPR005737
*  DNA topoisomerase III (IPR005738) | InterPro | EMBL-EBI
... topoisomerases I, III and V) break single-strand DNA, and type II enzymes (EC:5.99.1.3; topoisomerases II, IV and VI) break ... DNA topoisomerase, type IA, central region, subdomain 1 (IPR013824). *DNA topoisomerase, type IA, central region, subdomain 2 ( ... The type IB family of DNA topoisomerases includes eukaryotic nuclear topoisomerase I, topoisomerases of poxviruses, and ... Phylogenomics of type II DNA topoisomerases.. Bioessays 25 232-42 2003. Forterre P, Gribaldo S, Gadelle D, Serre MC. Origin and ...
https://www.ebi.ac.uk/interpro/entry/IPR005738
*  DNA topoisomerase VI, subunit B, transducer (IPR015320) | InterPro | EMBL-EBI
Phylogenomics of type II DNA topoisomerases.. Bioessays 25 232-42 2003. Watt PM, Hickson ID. Structure and function of type II ... GO:0006265 DNA topological change Molecular Function. GO:0003677 DNA binding GO:0003918 DNA topoisomerase type II (ATP- ... topoisomerases I, III and V) break single-strand DNA, and type II enzymes (EC:5.99.1.3; topoisomerases II, IV and VI) break ... type IIA (topoisomerase II or gyrase, and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for ...
http://www.ebi.ac.uk/interpro/entry/IPR015320
*  DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences. - PubMed - NCBI
DNA Topoisomerases, Type II. Grant support. *DK12121/DK/NIDDK NIH HHS/United States ... We have identified DNA gyrase [DNA topoisomerase (ATP-hydrolysing), EC 5.99.1.3] as one of the REP-binding proteins. Gyrase has ... Publication types, MeSH terms, Substance, Grant support. Publication types. *Research Support, Non-U.S. Gov't ... DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.. Yang Y1, Ames GF. ...
https://www.ncbi.nlm.nih.gov/pubmed/2848243
*  DNA Topoisomearases, Dna Topoisomerases:Part A: Biochemistry and Molecular Biology by J. Thomas August | 9780120329298 |...
Dna Topoisomerases:Part A: Biochemistry and Molecular Biology by J. Thomas August. Buy a discounted Hardcover of DNA ... Structure of Eukaryotic Type I DNA Topoisomerase. Type II DNA Topoisomerase Genes. ... and Religation Reactions of Eukaryotic Topoisomerases I and II. Roles of DNA Topoisomerases in Chromosomal Replication and ... DNA Topoisomearases. Dna Topoisomerases:Part A: Biochemistry and Molecular Biology. By: J. Thomas August (Editor), M.W. Anders ...
https://www.booktopia.com.au/dna-topoisomearases-j-thomas-august/prod9780120329298.html
*  Memory loan consolidation is defined with the stabilization of the storage - Therapeutic Applications of Kinase Inhibitors
Type II DNA topoisomerases have already been classified into two households,. Type II DNA topoisomerases have already been ... For BRL-49653 LTP, statistical evaluation was performed with two-way ANOVA with repeated steps. For all those analyses, ... Ribonucleotide reductase (RNR) is an integral enzyme that mediates the formation of deoxyribonucleotides, the DNA precursors, ... classified into two households, Topo IIA and Topo IIB, predicated on structural and […] ...
http://www.antibodyassay.com/index.php/2019/02/12/memory-loan-consolidation-is-defined-with-the-stabilization-of-the-storage/
*  DNA topoisomerase 4 subunit B (A0A0J9WZF0) | InterPro | EMBL-EBI
GO:0003677 DNA binding GO:0003918 DNA topoisomerase type II (ATP-hydrolyzing) activity GO:0005524 ATP binding ... DNA topoisomerase, type IIA (IPR001241)*DNA topoisomerase IV, subunit B, Gram-negative (IPR005737) ...
http://www.ebi.ac.uk/interpro/protein/A0A0J9WZF0
*  parE - DNA topoisomerase 4 subunit B - Rhodobacter capsulatus - parE gene & protein
It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. ... Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation. ,p>Information which has been generated ... DNA topoisomerase 4 subunit BUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation ... PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. ...
http://www.uniprot.org/uniprot/O68071
*  Eubacteria
Bacterial diversity based on type II DNA topoisomerase genes. Annual Review of Genetics 30:79-107. ... DNA polymerase C of the thermophilic bacterium Thermus aquaticus: Classification and phylogenetic analysis of the family C DNA ... For a more detailed explanation of the different ToL page types, have a look at the Structure of the Tree of Life page. ... The taxonomic outline for the 5 volumes, including all species, type strains, and 16S rRNA sequence data can be downloaded from ...
http://tolweb.org/tree?group=Eubacteria&contgroup=Life_on_Earth
*  DNA gyrase | definition of DNA gyrase by Medical dictionary
DNA gyrase explanation free. What is DNA gyrase? Meaning of DNA gyrase medical term. What does DNA gyrase mean? ... Looking for online definition of DNA gyrase in the Medical Dictionary? ... Related to DNA gyrase: Topoisomerase, Quinolones. DNA gyrase. (ji´rās) a type II DNA topoisomerase.. DNA gyrase. an enzyme that ... It functions by inhibiting DNA gyrase, a type II topoisomerase and topoisomerase IV enzymes necessary to division bacterial DNA ...
http://medical-dictionary.thefreedictionary.com/DNA+gyrase
*  fms-Like Tyrosine Kinase 3 Summary Report | CureHunter
Type II DNA Topoisomerases (Topoisomerase II) 10. PKC412 Related Therapies and Procedures. 1. Hematopoietic Stem Cell ... 2. Neoplasms (Cancer) 04/15/2015 - "An improved understanding of how the signaling pathways [e.g., Fms-like tyrosine kinase 3 ( ... Neoplasm Proteins: 2*Oncogene Proteins: 1748*Proto-Oncogene Proteins: 4*fms-Like Tyrosine Kinase 3: 223*human FLT3 protein ... CD135 Antigen; Fetal Liver Kinase-2; Fetal Liver Kinase-3; Stem Cell Tyrosine Kinase 1; Antigen, CD135; Fetal Liver Kinase 2; ...
http://www.curehunter.com/public/keywordSummaryD051941-fms-Like-Tyrosine-Kinase-3.do
*  C11orf80 Gene - GeneCards | TO6BL Protein | TO6BL Antibody
The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation ... Type 2 DNA topoisomerase 6 subunit B-like Protein Accession:. Q8N6T0. Secondary Accessions: *Q9H677 ... Isoform 3: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with SPO11, ... The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation ...
http://www.genecards.org/cgi-bin/carddisp.pl?gene=C11orf80
*  Frontiers | The Transcriptome of Streptococcus pneumoniae Induced by Local and Global Changes in Supercoiling | Microbiology
DNA-supercoiling is homeostatically maintained by the opposing activities of relaxing DNA topoisomerases and negative supercoil ... DNA-supercoiling acts as a general cis regulator of transcription, which can be superimposed upon other types of more specific ... DNA-supercoiling is homeostatically maintained by the opposing activities of relaxing DNA topoisomerases and negative supercoil ... DNA-supercoiling acts as a general cis regulator of transcription, which can be superimposed upon other types of more specific ...
https://www.frontiersin.org/articles/10.3389/fmicb.2017.01447/full
*  Protein sequence for HAGRID 80 (TOP2A)
DNA topoisomerase type II (ATP-hydrolyzing) activity. GO:0005080. protein kinase C binding. ...
http://genomics.senescence.info/genes/seq.php?id=80&type=cds
*  Study of Topoisomerase-DNA Interaction Using Atomic Force Microscopy - Infoscience
We found that human type II topoisomerases (Topo II) bind preferentially to DNA crossovers. About 50% of the DNA crossings, ... and evaluated the preferential binding of Topo II to specific DNA supercoiled forms. Finally, we explored the Topo II dynamics ... Our studies with AFM also helped us visualize the dynamics of the unknotting action of Topo II in knotted DNA molecules. ... Additionally we investigated the interactions between Topo II and DNA by applying single molecule force spectroscopy. This ...
https://infoscience.epfl.ch/record/174667