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*  Alterations in the DNA topoisomerase IV grlA gene responsible...
A 4.2-kb DNA fragment conferring quinolone res ... Alterations in the DNA topoisomerase IV grlA gene responsible ... These results suggest that both DNA gyrase and DNA topoisomerase IV may be targets of quinolones and that the quinolone ... Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus.. Authors * J ... A 4.2-kb DNA fragment conferring quinolone resistance was cloned from a quinolone-resistant clinical isolate of Staphylococcus ...
https://www.mysciencework.com/publication/show/alterations-in-the-dna-topoisomerase-iv-grla-gene-responsible-for-quinolone-resistance-in-staphylococcus-aureus
*  PROSITE
TOPOISOMERASE_I_PROK, PS00396; Prokaryotic DNA topoisomerase I active site (PATTERN). * Consensus pattern:. [EQ]-x-L-Y-[DEQSTLM ... When a prokaryotic type I topoisomerase breaks a DNA backbone bond, it simultaneously forms a protein-DNA link where the ... interconversion of topological DNA isomers. Type I topoisomerases act by catalyzing the transient breakage of DNA, one strand ... DNA topoisomerase I (EC 5.99.1.2) [1,2,3,4] is one of the two types of enzyme that catalyze the ...
http://prosite.expasy.org/PDOC00333
*  Topoisomerase II Alpha - Product: Leica Biosystems
Topoisomerase II alpha is an essential nuclear enzyme involved in DNA replication and is a target for many anti-cancer drugs ... Topoisomerase II alpha expression and activity is linked to the cell cycle and is associated with the proliferation status of ... Decreased expression of topoisomerase II alpha is the predominant mechanism of resistance to several chemotherapeutic agents. A ... Reports of the analysis of primary breast tumors have indicated that topoisomerase II beta is more widely expressed than ...
https://www.leicabiosystems.com/ihc-ish-fish/immunohistochemistry-ihc-antibodies-novocastra-reagents/primary-antibodies/products/topoisomerase-ii-alpha/
*  Teniposide - DrugBank
This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA ... Teniposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. ... Teniposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double ... Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes ...
https://www.drugbank.ca/drugs/DB00444
*  DNA topoisomerase 4 subunit B (A0A0J9WZF0) | InterPro | EMBL-EBI
DNA topoisomerase, type IIA (IPR001241)*DNA topoisomerase IV, subunit B, Gram-negative (IPR005737) ... GO:0003677 DNA binding GO:0003918 DNA topoisomerase type II (ATP-hydrolyzing) activity GO:0005524 ATP binding ...
http://www.ebi.ac.uk/interpro/protein/A0A0J9WZF0
*  DNA Topoisomerase IV - Semantic Scholar
Topoisomerase IV binds to DNA as a heterotetramer consisting 2 parC and 2 parE subunits. Topoisomerase IV is a decatenating ... A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands ... enzyme that resolves interlinked daughter chromosomes following DNA replication. ... DNA Topoisomerase IV. Known as: Topoisomerase IV, DNA, Topo IV, DNA Topoisomerase IV [Chemical/Ingredient] (More). ...
https://www.semanticscholar.org/topic/DNA-Topoisomerase-IV/508540
*  DNA topoisomerase IV, subunit B, Gram-negative (IPR005737) | InterPro | EMBL-EBI
DNA topoisomerase, type IIA (IPR001241) *DNA topoisomerase IV, subunit B, Gram-negative (IPR005737) ... of topoisomerase IV from Gram negative bacteria. Topoisomerase IV primarily decatenates DNA and relaxes positive supercoils, ... topoisomerases I, III and V) break single-strand DNA, and type II enzymes (EC:5.99.1.3; topoisomerases II, IV and VI) break ... and topoisomerase IV) and type IIB (topoisomerase VI). These enzymes are responsible for relaxing supercoiled DNA as well as ...
http://www.ebi.ac.uk/interpro/entry/IPR005737
*  parE - DNA topoisomerase 4 subunit B - Rhodobacter capsulatus - parE gene & protein
It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. ... Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required ... DNA topoisomerase 4 subunit BUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation ... Topoisomerase IV subunit BUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation ...
http://www.uniprot.org/uniprot/O68071
*  RCSB PDB - Protein Feature View - DNA topoisomerase 4 subunit A - P72525 (PARC STRPN)
Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA (PubMed:17375187, PubMed:20596531). ... Performs the decatenation events required during the replication of a circular DNA molecule. UniProt ... P72525 - Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) 17 * P20082 - Escherichia coli (strain K12) no ...
http://www.rcsb.org/pdb/protein/P72525
*  Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in...
DNA topoisomerase IV mediates chromosome segregation and is a potential target for antibacterial agents including new ... To examine the role of DNA topoisomerase IV in fluoroquinolone action and resistance in S. pneumoniae, we isolated mutant ... These results suggest that DNA topoisomerase IV is an important target for fluoroquinolones in S. pneumoniae and establish this ... Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in ...
https://jb.asm.org/content/178/14/4060?ijkey=9db78851df0f4636e183bf13cb088149e427e30e&keytype2=tf_ipsecsha
*  RCSB PDB - 4GGL: Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and...
Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and optimization of dual ... The bacterial topoisomerases DNA gyrase (GyrB) and topoisomerase IV (ParE) are essential enzymes that control the topological ... The bacterial topoisomerases DNA gyrase (GyrB) and topoisomerase IV (ParE) are essential enzymes that control the topological ... Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and optimization of dual ...
https://www.rcsb.org/structure/4GGL
*  3D View - 4GEE: Pyrrolopyrimidine inhibitors of DNA gyrase B and topoisomerase IV, part I: structure guided discovery...
... and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad- ... Pyrrolopyrimidine inhibitors of DNA gyrase B and topoisomerase IV, part I: structure guided discovery and optimization of dual ... 1R,5S,6s)-3-[5-chloro-6-ethyl-2-(pyrimidin-5-yloxy)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]-3-azabicyclo[3.1.0]hexan-6-amine. C17 H18 ... Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) ...
http://www.rcsb.org/pdb/explore/jmol.do?structureId=4GEE&bionumber=1
*  RCSB PDB - 4HYM: Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided...
... and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad- ... Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and optimization of dual ... Topoisomerase IV, subunit B A, B 390 Francisella tularensis EC#: 5.99.1.3 IUBMB Mutation: L258P Gene Name(s): parE FTL_1726 ... 7-({4-[(3R)-3-aminopyrrolidin-1-yl]-5-chloro- 6-ethyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl}sulfanyl)pyrido[2,3- b]pyrazin-2(1H)-one ...
http://www.rcsb.org/pdb/explore/explore.do?structureId=4HYM
*  Structure Cluster - 4GGL: Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided...
... and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad- ... Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and optimization of dual ... Description: DNA gyrase subunit B protein , Length: 215 No structure alignment results are available for 4GGL.A explicitly.. It ... 4] Alexandrov N., Shindyalov I. (2003). PDP: protein domain parser.. Bioinformatics 2003 Feb;19(3);429-30. ...
http://www.rcsb.org/pdb/explore/structureCluster.do?structureId=4GGL
*  RCSB PDB - 3RAF: Quinazolinedione-DNA cleavage complex of type IV topoisomerase from S. pneumoniae Structure Summary...
Inhibitor-stabilised cleavage complexes of topoisomerase IIa: structural analysis of drug-dependent inter- and intramolecular ... Quinazolinedione-DNA cleavage complex of type IV topoisomerase from S. pneumoniae. *DOI: 10.2210/pdb3raf/pdb ... DNA topoisomerase 4 subunit A A, B 496 Streptococcus pneumoniae EC#: 5.99.1.3 IUBMB Fragment: UNP residues 1-488 Mutation: ... DNA topoisomerase 4 subunit B C, D 268 Streptococcus pneumoniae EC#: 5.99.1.3 IUBMB Fragment: UNP residues 404-647 Mutation: ...
http://www.rcsb.org/pdb/explore.do?structureId=3RAF
*  Anticipating the Unpredictable: A Review of Antimicrobial Stewardship and Acinetobacter Infections | SpringerLink
DNA gyrase alterations. Fluoroquinolones. ParC. DNA topoisomerase IV alterations. Fluoroquinolones. RpoB. RNA polymerase ... four case reports and an in vitro combination synergy study. Pharmacotherapy. 2007;27:1506-11.PubMedCrossRefGoogle Scholar ... and was active against four of the six tigecycline-resistant strains tested. After addition to formulary, the ASP then ... 4.. Nelson RE, Schweizer ML, Perencevich EN, Nelson SD, Khader K, Chiang HY, Chorazy ML, Blevins A, Ward MA, Samore MH. Costs ...
https://link.springer.com/article/10.1007%2Fs40121-017-0149-y
*  Université de Rennes 1 - Role of DNA gyrase and topoisomerase IV mutations in fluoroquinolone resistance of Capnocytophaga spp....
Other mutations in parC and parE led to spontaneous amino acid substitutions of DNA topoisomerase IV subunit B with no ... and topoisomerase IV (parC and parE) of fluoroquinolone (FQ)-resistant Capnocytophaga spp., hitherto unknown, was explored in ... The implication of mutations in the quinolone resistance-determining region (QRDR) of DNA gyrase A and B (gyrA and gyrB) ... Methods: Two reference strains (Capnocytophaga gingivalis ATCC 33624 and Capnocytophaga sputigena ATCC 33612) and four ...
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578619v1
*  3FOE: Structural Insight Into The Quinolone-Dna Cleavage Complex Of Type Iia Topoisomerases
DNA (5'- D(P*AP*GP*TP*CP*AP*TP*TP*CP*AP*TP*GP*AP*CP*CP*TP*TP*GP*GP*T )-3')DNA (5'- D(P*CP*TP*GP*TP*TP*TP*TP*AP*CP*GP*TP*GP*CP* ... DNA (5'- D(P*AP*CP*CP*AP*AP*GP*GP*TP*CP*AP*TP*GP*AP*AP*T)-3') ... DNA (5'- D(p*ap*gp*tp*cp*ap*tp*tp*cp*ap*tp*gp*ap*cp*cp*tp*tp*gp ... 3FOE: Structural Insight Into The Quinolone-Dna Cleavage Complex Of Type Iia Topoisomerases. ... DNA (5'- D(p*gp*ap*cp*tp*ap*tp*gp*cp*ap*cp*gp*tp*ap*ap*ap*ap*cp*ap*g )-3') ...
https://www.ncbi.nlm.nih.gov/Structure/pdb/3FOE
*  PDB 4lp0 structure summary ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI
Fragment-to-Hit-to-Lead Discovery of a Novel Pyridylurea Scaffold of ATP Competitive Dual Targeting Type II Topoisomerase ... DNA topoisomerase, type IIA * Occurring in:. *DNA topoisomerase 4 subunit B. '> DNA topoisomerase 4 subunit B, Firmicutes/ ... Novel compounds targeting bacterial DNA topoisomerase/DNA gyrase. Ehmann et al. (2014) ... DNA topoisomerase 4 subunit B Chain: A Molecule details › Chain: A. Length: 226 amino acids. Theoretical weight: 24.6 KDa. ...
http://www.ebi.ac.uk/pdbe/entry/pdb/4lp0
*  IUCr) Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline
Holliday junction DNA helicase B. Insoluble. ++. +. Bartonella henselae. YP_033595.1. Full length. DNA topoisomerase IV subunit ... Holliday junction DNA helicase B. No expression. ++. ++. Brucella melitensis. YP_419002. Full length. Catalase. Insoluble. ++. ... DNA templates were obtained from the SSGCID pipeline (Myler et al., 2009. ). Following E. coli in vivo expression trials, PCR ... In each of the reaction tubes, 2 µg plasmid DNA was mixed with transcription buffer (80 mM HEPES-KOH pH 7.8 containing 20 mM ...
http://journals.iucr.org/f/issues/2011/09/00/en5477/index.html
*  SMART: TOP4c domain annotation
Bacterial DNA topoisomerase IV, GyrA, ParC. Interpro abstract (IPR002205):. DNA topoisomerases regulate the number of ... DNA cleavage complex of type IV topoisomerase from S. pneumoniae. 3rae. Quinolone(Levofloxacin)-DNA cleavage complex of type IV ... Human Topoisomerase Iibeta in complex with DNA. 4juo. A low-resolution three-gate structure of topoisomerase IV from ... Human topoisomerase IIbeta in complex with DNA and amsacrine. 4g0v. Human topoisomerase iibeta in complex with DNA and ...
http://smart.embl-heidelberg.de/smart/do_annotation.pl?ACC=SM00434&BLAST=DUMMY
*  ASMscience | Identification of Mar Mu
DNA Topoisomerase IV. 0.43724346. Point Mutation. 0.4351051. 0.5054011. Highlighted Text: Show , Hide ... Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli. Antimicrob. Agents Chemother. 35:1647- ... Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants of Escherichia coli. Antimicrob. Agents ... Ineffectiveness of topoisomerase mutations in mediating clinically significant fluoroquinolone resistance in Escherichia coli ...
http://www.asmscience.org/content/book/10.1128/9781555817572.chap16
*  Grepafloxacin - DrugBank
Dna topoisomerase type ii (atp-hydrolyzing) activity. Specific Function. Topoisomerase IV is essential for chromosome ... Grepafloxacin exerts its antibacterial activity by inhibiting bacterial topoisomerase II (DNA gyrase) and topoisomerase IV, ... Dna topoisomerase type ii (atp-hydrolyzing) activity. Specific Function. DNA gyrase negatively supercoils closed circular ... Details2. DNA topoisomerase 4 subunit A. Kind. Protein. Organism. Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 ...
https://www.drugbank.ca/drugs/DB00365
*  Cited In for PubMed (Select 1658161) - PubMed - NCBI
Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus. ... Inhibitory activities of quinolones against DNA gyrase and topoisomerase IV purified from Staphylococcus aureus. ... Role of mutations in DNA gyrase genes in ciprofloxacin resistance of Pseudomonas aeruginosa susceptible or resistant to ... Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA gene from strain PAO1 and quinolone-resistant ...
https://www.ncbi.nlm.nih.gov/pubmed?linkname=pubmed_pubmed_citedin&from_uid=1658161
*  Mutations in Topoisomerase IV and DNA Gyrase ofStaphylococcus aureus: Novel Pleiotropic Effects on Quinolone and Coumarin...
... topoisomerase IV is considerably less sensitive to novobiocin than DNA gyrase. In the gyrB142 background, topoisomerase IV may ... encodes one of the two subunits of DNA topoisomerase IV. The grlB gene encoding the second subunit of topoisomerase IV has not ... Mutations in Topoisomerase IV and DNA Gyrase ofStaphylococcus aureus: Novel Pleiotropic Effects on Quinolone and Coumarin ... Mutations in Topoisomerase IV and DNA Gyrase ofStaphylococcus aureus: Novel Pleiotropic Effects on Quinolone and Coumarin ...
https://aac.asm.org/content/42/1/121?ijkey=95da74c5ebe7802e09e8e07bdd14033324573cfc&keytype2=tf_ipsecsha