DNA_LIGASE_A2 (PS00333). Accession PS00333 Integration. DNA ligase, ATP-dependent, conserved site (IPR016059) Member database. ...
View a chart of NEBs DNA and RNA ligases, their differences, and recommendations for optimal performance. ... Hi-T4 DNA Ligase displays increased thermotolerance compared to T4 DNA Ligase. Hi-T4 DNA Ligase can withstand temperatures as ... See also notes for T4 DNA Ligase.. T3 DNA Ligase. M0317S/L. 25°C. (4-37°C). N. ATP. A salt-tolerant enzyme for the ligation of ... Home Tools & Resources Selection Charts Properties of DNA and RNA Ligases Properties of DNA and RNA Ligases. NEB offers many ...
As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a ... Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. ... for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology ... It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. ...
DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in ... It can also ligate blunt-ended DNA with much greater efficiency than E. coli DNA ligase. Unlike E. coli DNA ligase, T4 DNA ... Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA. The mechanism of DNA ligase ... it has been recognized as another DNA ligase and it is the reason for the unusual nomenclature of DNA ligases. DNA ligase from ...
DNA recombination and DNA repair in an ATP-dependent reaction. Binds specifically to DNA nicks containing a 3-OH and a 5- ... DNA ligase that seals nicks in double-stranded DNA during DNA replication, ... IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR016306. DNA_ligase_ATP-dep_T3. IPR012340. NA-bd_OB- ... IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR016306. DNA_ligase_ATP-dep_T3. IPR012340. NA-bd_OB- ...
IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR036599. DNA_ligase_N_ ... IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR036599. DNA_ligase_N_ ... PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. ... PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. ...
Rabbit polyclonal DNA Ligase III antibody validated for WB, IHC, ICC/IF and tested in Human, Mouse and Rat. Referenced in 1 ... Anti-DNA Ligase III antibody. See all DNA Ligase III primary antibodies. ... Anti-DNA Ligase III antibody (ab185815) at 1/500 dilution + extracts of testis cell lines. Developed using the ECL technique. ... Belongs to the ATP-dependent DNA ligase family.. Contains 1 BRCT domain.. Contains 1 PARP-type zinc finger. ...
Rabbit polyclonal DNA Ligase III antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen ... Anti-DNA Ligase III antibody. See all DNA Ligase III primary antibodies. ... Anti-DNA Ligase III antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg. Predicted band size: 113 kDa. 7.5 ... Recombinant fragment containing a sequence corresponding to a region within amino acids 116-403 of Human DNA Ligase III (NP_ ...
T4 DNA Ligase. T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5 phosphate and 3 hydroxyl ... Blunt and cohesive end DNA ligation*Blunt end linker addition*Single-strand DNA, RNA and DNA/RNA repair *DNA fragment insertion ... as well as single-stranded nick repair of DNA, RNA and DNA/RNA, are possible via the T4 DNA ligase. This enzyme is supplied in ... One ligation unit is defined as the amount of enzyme that ligates ,90% of 6 µg of Hind III-restricted lambda DNA in a 20 µL ...
Mouse monoclonal DNA Ligase I antibody [1A9] validated for WB, ELISA and tested in Human. Referenced in 1 publication and 1 ... DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. ... Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.. Mol Cell Biol 14:310-7 ( ... Anti-DNA Ligase I antibody [1A9]. See all DNA Ligase I primary antibodies. ...
In addition, our data implicate mammalian ligase IV in V(D)J recombination and the repair of radiation-induced DNA damage, … ... Our findings provide a function for the carboxy-terminal region of ligase IV and suggest that BRCT domains of other proteins ... Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV Curr Biol. 1997 Aug 1;7(8):588-98. doi: ... In addition, our data implicate mammalian ligase IV in V(D)J recombination and the repair of radiation-induced DNA damage, and ...
View Mouse Monoclonal anti-DNA Ligase I Antibody (7A12) (NB100-2714). Validated Applications: WB, ELISA, IP. Validated Species ... Home » DNA Ligase I » DNA Ligase I Antibodies » DNA Ligase I Antibody (7A12) ... Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is ... Reviews for DNA Ligase I Antibody (NB100-2714) (0) There are no reviews for DNA Ligase I Antibody (NB100-2714). By submitting a ...
Polynucleotide Ligase,T4 DNA Ligase; find Sigma-Aldrich-D2886 MSDS, related peer-reviewed papers, technical documents, similar ... DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution; CAS Number: 9015-85-4; EC Number: 232-770-0; ... T4 DNA Ligase. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ ... T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. ...
DNA replication is strictly regulated to occur only once per cell cycle. In eukaryotes, the presence of licensing proteins at ... CUL-4 Ubiquitin Ligase Maintains Genome Stability by Restraining DNA-replication Licensing Nature. 2003 Jun 19;423(6942):885-9. ... Here we show that the CUL-4 ubiquitin ligase temporally restricts DNA-replication licensing in Caenorhabditis elegans. ... Inactivation of CUL-4 causes massive DNA re-replication, producing cells with up to 100C DNA content. The C. elegans orthologue ...
nothing! i checked the t4 dna ligase bottle from th... ... Thermo scientific T4 DNA ligase product help - posted in ... I used NEB T4 DNA ligase instead. I suppose its fine as long as you put more ligase than it should be. I used 1 ul as well. ... I used NEB T4 DNA ligase instead. I suppose its fine as long as you put more ligase than it should be. I used 1 ul as well. ... Also tagged with one or more of these keywords: ligation, T4 dna ligase. Protocols and Techniques Forums → Molecular Cloning → ...
Alternative names for DNA ligase 1 antibody. LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I, Polydeoxyribonucleotide ... Background of DNA ligase 1 antibody. Kit Component:. - KN309280G1, Lig1 gRNA vector 1 in pCas-Guide vector. - KN309280G2, Lig1 ... Western blot (WB) analysis of DNA Ligase I antibody (Cat.-No.: AP20546PU-N) in extracts from HT-29 cells.. *AP20546PU-N ... Immunocytochemical detection of DNA Ligase I on acetone fixed HEp-II cells with AM26565AF-N.. *AM26565AF-N ...
... ligase I, DNA, ATP-dependent) for IP, WB. Anti-DNA ligase I mAb (GTX70141) is tested in Human, Mouse samples. 100% Ab-Assurance ... The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair ... DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also ... Detection of human Ligase I protein in Raji whole cell lysate using GeneTex DNA Ligase I 10H5 monoclonal antibody GTX70141. ...
dpl:category=Material,category=Enzyme,category=Ligase,category=DNA,nottitlematch=DNA ligase}} ... dpl:category=Material,category=Enzyme,category=Ligase,category=DNA,nottitlematch=DNA ligase}} ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=DNA_ligase&oldid=203316" ...
Add 0.5 μL ligase. Vortex ligase before pipetting to ensure that it is well-mixed. Also, the ligase, like most enzymes, is in ... 0.5 μL T4 DNA ligase. *Reshma 19:12, 13 December 2007 (CST): I frequently digest 500 ng of each part and the destination vector ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Knight:DNA_ligation_using_T4_DNA_ligase&oldid=175735" ...
This article is about general ligases. For DNA specific ligases, see DNA ligase. ... The common names of ligases often include the word "ligase", such as DNA ligase, an enzyme commonly used in molecular biology ... DNA ligase. References[edit]. *^ "Synthases and ligases". chem.qmul.ac.uk. Archived from the original on October 15, 2012. ... Ligase can join two complementary fragments of nucleic acid and repair single stranded breaks that arise in double stranded DNA ...
Single Strand DNA Ligase, thermostable, recombinant, Solution \ 298-65103 for more molecular products just contact us ... Single Strand DNA Ligase, thermostable, recombinant, Solution. Related products : Single Strand DNA Ligase, thermostable, ... Single Strand DNA Ligase, thermostable, recombinant, Solution / Product Detail : 298-65103 Single Strand DNA Ligase, ... Single Strand DNA Ligase, thermostable, recombinant, Solution recombinant proteins Most stable storage is at - 81 C or lower ...
T4 DNA Ligase - N_A N_A \ 10-663-45311 for more molecular products just contact us ... We have also other products like : T4 DNA Ligase - N_A N_A. Related products : T4 DNA Ligase - N_A N_A ... 43] DNA-directed DNA polymerase (EC 2.7.7.7) (EC 3.1.11.-) (Gene product 43) (Gp43). [E] Endolysin (EC 3.2.1.17) (Lysis protein ... 10-663-45311 T4 DNA Ligase - N_A N_A Ask technical file . ... T4 DNA Ligase - N_A N_A / Product Detail : 10-663-45311 T4 DNA ...
... hydroxyl termini in duplex DNA containing cohesive ends. ... coli DNA Ligase (NEB# M0205) differ from T4 DNA Ligase (NEB# ... E. coli DNA Ligase Reaction Buffer -20 10 X Product Categories:. DNA Ligases Products. Applications: Non-Cloning Ligation. * ... 1X E. coli DNA Ligase Reaction Buffer Incubate at 16°C 1X E. coli DNA Ligase Reaction Buffer 30 mM Tris-HCl 4 mM MgCl2 26 µM ... coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4 ...
At the heart of this repair pathway is Xrcc4-DNA ligase IV complex, which mediates ligation of the broken DNA strands. The C- ... p, ,p, The structure maintains a 2:1 stoichiometry of Xrcc4 to the C-terminal domain of DNA ligase IV. The structure of the ... The crystal structure of Xrcc4-DNA ligase IV complex was solved by single-wavelength anomalous diffraction using data collected ... Structural Characterization of the C-terminal Domain of Human DNA Ligase IV Bound to Xrcc4. ...
Enzymes called DNA ligases can rejoin cut or broken DNA strands.[120] Ligases are particularly important in lagging strand DNA ... DNA exists in many possible conformations that include A-DNA, B-DNA, and Z-DNA forms, although, only B-DNA and Z-DNA have been ... Further information: DNA supercoil. DNA can be twisted like a rope in a process called DNA supercoiling. With DNA in its " ... Branched DNA. Further information: Branched DNA and DNA nanotechnology. In DNA, fraying occurs when non-complementary regions ...
The SCOP classification for the Adenylation domain of NAD+-dependent DNA ligase family. Additional information, provided for ... DNA ligase/mRNA capping enzyme, catalytic domain [. 56091] (4) Family: Adenylation domain of NAD+-dependent DNA ligase [. 56096 ... Ligases that form phosphoric-ester bonds. 1. Informative. Inherited. Enzyme Commission (EC). DNA ligase (NAD(+)). 0. Highly ... Ligases that form phosphoric-ester bonds. 1. 1.741. Informative. INHERITED FROM: DNA ligase (NAD(+)). ...
All DNA ligases contain an essential lysine that transfers an adenylate group from a co-factor to the 5′-phosphate of the DNA ... DNA ligases are enzymes that seal breaks in the backbones of DNA, leading to them being essential for the survival of all ... DNA ligases have been studied from many different types of cells and organisms and shown to have diverse sizes and sequences, ... The essential DNA ligases in bacteria use β-nicotinamide adenine dinucleotide (β-NAD+) as their co-factor whereas those that ...
The researchers were interested in bacterial DNA ligase (LigA), which is essential for DNA replication, is highly conserved ...
DNA-binding assays revealed that both the polymerase and ligase domains bind DNA in vitro, the latter with considerably higher ... The ligase domain catalysed the sealing of nicked double-stranded DNA designed to mimic a DSB, consistent with the role of Mt- ... Amino Acid Sequence Bacterial Proteins/metabolism Base Sequence Binding Sites DNA Primers DNA Repair Electrophoresis, ... Domain structure of a NHEJ DNA repair ligase from Mycobacterium tuberculosis. Journal of Molecular Biology, 351 (3). pp. 531-44 ...
I developed several assays to assess the functional consequences of DNA ligase IV mutations (interaction with XRCC4, ability to ... DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency ... DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency. Molecular Cell, 8 (6). pp. ... This paper describes the identification of a novel DNA repair syndrome, termed LIG4 syndrome.. ...