Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties - Lakaye Bernard
Cloning, Molecular ; DNA, Complementary/genetics ; Diethyl Pyrocarbonate/chemistry ; Enzyme Activation ; Enzyme Stability ; ... The effects of group-specific reagents such as Woodwards reagent K and diethylpyrocarbonate suggest that at least one carboxyl ... en] thiamine triphosphate ; Fourier-transform infrared spectroscopy ; zinc ions ; diethylpyrocarbonate ; Woodwards reagent K. ...http://orbi.ulg.ac.be/handle/2268/4630
Diethyl pyrocarbonate - Wikipedia
Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase ... A General Reaction of Diethylpyrocarbonate with Proteins". European Journal of Biochemistry. 13 (3): 519-25. doi:10.1111/j.1432 ... "A Mechanism of the Irreversible Inactivation of Bovine Pancreatic Ribonuclease by Diethylpyrocarbonate. ...https://en.wikipedia.org/wiki/Diethyl_pyrocarbonate
253. Diethyl pyrocarbonate (WHO Food Additives Series 4)
Diethyl pyrocarbonate (FAO Nutrition Meetings Report Series 40abc) Diethyl pyrocarbonate (WHO Food Additives Series 5) DIETHYL ... DIETHYL PYROCARBONATE Biological data Biochemical aspects Diethyl pyrocarbonate is rapidly hydrolyzed by water with the ... Measurements using 14C-labelled diethyl carboxylation of constituents of the beverages, when 100 mg diethyl pyrocarbonate was ... diethyl pyrocarbonate, the mixture being permitted to stand two days before use so that the pyrocarbonate was completely ...http://www.inchem.org/documents/jecfa/jecmono/v004je06.htm
Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying...
Diethyl pyrocarbonate (DEPC), a histidine-modifying reagent, has been utilized to demonstrate the importance of histidine ... Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying ...https://portlandpress.com/biochemj/crossref-citedby/39331
Ultrapure Biochemicals - Alfa Aesar
Diethyl pyrocarbonate, 97+%, Ultrapure, Thermo Scientific. ×Close. Diethyl pyrocarbonate, 97+%, Ultrapure, Thermo Scientific ...https://www.alfa.com/en/ultrapure_biochemicals/
4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral...
The total cellular RNA was pelleted by centrifugation and redissolved in diethyl pyrocarbonate (DEPC-) treated . The ...https://www.hindawi.com/journals/ecam/2013/435916/
Modeling of a mutation responsible for human 3-hydroxy-3-methylglutaryl-CoA lyase deficiency implicates histidine 233 as an...
... lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored by incubation with hydroxylamine. ... Diethyl Pyrocarbonate / pharmacology. Enzyme Inhibitors / pharmacology. Histidine / metabolism*. Humans. Hydrolysis. Metabolism ... 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored ... 0/Acyl Coenzyme A; 0/Enzyme Inhibitors; 1553-55-5/3-hydroxy-3-methylglutaryl-coenzyme A; 1609-47-8/Diethyl Pyrocarbonate; 71-00 ...http://www.biomedsearch.com/nih/Modeling-mutation-responsible-human-3/8798725.html
The effect of chemical modification of basic amino acid residues on the activation and amidolytic activity of Hageman factor ...
Diethyl Pyrocarbonate / pharmacology. Factor XII / antagonists & inhibitors, chemistry*, metabolism. Factor XIIa / metabolism. ... 0/Amides; 0/Amino Acids; 0/Serum Albumin, Bovine; 1074-12-0/Phenylglyoxal; 1609-47-8/Diethyl Pyrocarbonate; 2508-19-2/ ...http://www.biomedsearch.com/nih/effect-chemical-modification-basic-amino/8245689.html
... diethyl pyrocarbonate; LRP, lung resistance-related protein; BCRP, breast cancer resistance protein; ABC, ATP-binding cassette ...http://clincancerres.aacrjournals.org/content/9/2/827
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum | Protocol ...
Diethyl pyrocarbonate. Sigma. D5758. DEPC. DEPC-treated water (see REAGENT SETUP). Yoyo-1 DNA fluorescent dye. Thermo Fisher. ...https://www.jove.com/video/53214/metoder-til-undersgelse-af-regulatory-rolle-sm-rna-og-ribosomal?language=Danish
Expansins Are Conserved in Conifers and Expressed in Hypocotyls in Response to Exogenous Auxin | Plant Physiology
diethyl pyrocarbonate. IBA. indole-3-butyric acid. NPA. N-(1-naphthyl)phthalamic acid. RACE. rapid amplification of cDNA ends. ...http://www.plantphysiol.org/content/120/3/827?ijkey=e5dc330a1ebcee84a437bf3c8f1e7951d06b9b41&keytype2=tf_ipsecsha
Nuclease-Free Water at Your Fingertips | Sigma-Aldrich
Diethyl pyrocarbonate - Product information sheet; /content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/d5758pis.pdf ... Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this ... A Mechanism of the Irreversible Inactivation of Bovine Pancreatic Ribonuclease by Diethylpyrocarbonate, European Journal of ...https://www.sigmaaldrich.com/technical-documents/articles/biology/water-purification-systems/biomedical-applications/nuclease-free-water.html
Prevalence of dengue and chikungunya virus infections in north-eastern Tanzania: a cross sectional study among participants...
RNA was eluted from the silica pellets in 50 μl of diethyl pyrocarbonate (DEPEC) treated water and incubated for 10 min at 56 ° ...https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-016-1511-5
Enzymic characterization with progress curve analysis of a collagen peptidase from an enthomopathogenic bacterium, Photorhabdus...
Abbreviations used: AMC, 7-amino-4-methylcoumarin; But, t-butyl; DEPC, diethyl pyrocarbonate; DMF, dimethylformamide; DTT, ...http://www.biochemj.org/content/379/3/633
Preparation of Short Interfering RNA Containing the Modified Nucleosides 2‐Thiouridine, Pseudouridine, or Dihydrouridine -...
Diethyl pyrocarbonate (DEPC; Aldrich). * Milli‐Q water. * Automated oligonucleotide synthesizer (Applied Biosystems 394 or ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-nc1602.html
Subtracting additives | Michigan Today
Diethyl pyrocarbonate (DEPC) - preservative (beverages). Carcinogen (1972). Dulcin (p-ethoxy-phenylurea) - artificial sweetener ...https://michigantoday.umich.edu/2018/03/19/subtracting-additives/
Mechanism of Resistance to Dietary Cholesterol
Total RNA was resuspended in diethyl pyrocarbonate-treated water. Twenty micrograms of RNA was then DNAse-treated using the ...https://www.hindawi.com/journals/jl/2011/101242/
Synthesis of Site‐Specifically Phosphate‐Caged siRNAs - Current Protocols
Diethyl pyrocarbonate (DEPC) treated water (mix 1:1000 DEPC/HPLC‐grade water; sterilize at high temperature) ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-nc0612.html?quicktabs_cp=toc
Bacterial magnetosomes as an efficient gene delivery platform for cancer theranostics | Microbial Cell Factories | Full Text
... were prepared by mixing a certain amount of siRNA with PEI in diethyl pyrocarbonate (DEPC) water, and fixed amounts of BMs were ...https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0830-6
RNA Protocols & Applications - QIAGEN
Alternatively, glassware can be treated with DEPC (diethyl pyrocarbonate). Fill glassware with 0.1% DEPC (0.1% in water), ...https://www.qiagen.com/kr/resources/molecular-biology-methods/rna/
RNA Protocols & Applications - QIAGEN
Alternatively, glassware can be treated with DEPC (diethyl pyrocarbonate). Fill glassware with 0.1% DEPC (0.1% in water), ...https://www.qiagen.com/ke/resources/molecular-biology-methods/rna/
... of random primer and diethyl pyrocarbonate-treated water to total volume 25 μl, then heated the mixture at 65°C for 5 min and ... and 6 μl of diethyl pyrocarbonate-treated water. The samples were incubated at 42°C for 2 min. Then 1 μl of Superscript II (40 ...http://clincancerres.aacrjournals.org/content/10/15/4944
Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase. - PubMed - NCBI
SP activity was inhibited by thioreactive agents, diethyl pyrocarbonate, phenylglyoxal, and ADP-glucose. The activation ...https://www.ncbi.nlm.nih.gov/pubmed/11361132?dopt=Abstract
Different Roles of EIIABMan and EIIGlc in Regulation of Energy Metabolism, Biofilm Development, and Competence in Streptococcus...
RNA was resuspended in 52 μl of diethyl pyrocarbonate-treated water; 1 μl was used to estimate RNA concentration, and 1 μl was ... Pelleted cells were resuspended in 400 μl of diethyl pyrocarbonate-treated water, 800 μl of RNA protect reagent (QIAGEN, Inc., ...https://jb.asm.org/content/188/11/3748?ijkey=55e929046a845023f3f2882e5a9eae31f709fabd&keytype2=tf_ipsecsha