Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties - Lakaye BernardHuman recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties - Lakaye Bernard

Cloning, Molecular ; DNA, Complementary/genetics ; Diethyl Pyrocarbonate/chemistry ; Enzyme Activation ; Enzyme Stability ; ... The effects of group-specific reagents such as Woodwards reagent K and diethylpyrocarbonate suggest that at least one carboxyl ... en] thiamine triphosphate ; Fourier-transform infrared spectroscopy ; zinc ions ; diethylpyrocarbonate ; Woodwards reagent K. ...
more infohttp://orbi.ulg.ac.be/handle/2268/4630

Diethyl pyrocarbonate - WikipediaDiethyl pyrocarbonate - Wikipedia

Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase ... A General Reaction of Diethylpyrocarbonate with Proteins". European Journal of Biochemistry. 13 (3): 519-25. doi:10.1111/j.1432 ... "A Mechanism of the Irreversible Inactivation of Bovine Pancreatic Ribonuclease by Diethylpyrocarbonate. ...
more infohttps://en.wikipedia.org/wiki/Diethyl_pyrocarbonate

253. Diethyl pyrocarbonate (WHO Food Additives Series 4)253. Diethyl pyrocarbonate (WHO Food Additives Series 4)

Diethyl pyrocarbonate (FAO Nutrition Meetings Report Series 40abc) Diethyl pyrocarbonate (WHO Food Additives Series 5) DIETHYL ... DIETHYL PYROCARBONATE Biological data Biochemical aspects Diethyl pyrocarbonate is rapidly hydrolyzed by water with the ... Measurements using 14C-labelled diethyl carboxylation of constituents of the beverages, when 100 mg diethyl pyrocarbonate was ... diethyl pyrocarbonate, the mixture being permitted to stand two days before use so that the pyrocarbonate was completely ...
more infohttp://www.inchem.org/documents/jecfa/jecmono/v004je06.htm

Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying...Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying...

Diethyl pyrocarbonate (DEPC), a histidine-modifying reagent, has been utilized to demonstrate the importance of histidine ... Inhibition of the neutrophil NADPH oxidase and associated H+ channel by diethyl pyrocarbonate (DEPC), a histidine-modifying ...
more infohttps://portlandpress.com/biochemj/crossref-citedby/39331

Ultrapure Biochemicals - Alfa AesarUltrapure Biochemicals - Alfa Aesar

Diethyl pyrocarbonate, 97+%, Ultrapure, Thermo Scientific. ×Close. Diethyl pyrocarbonate, 97+%, Ultrapure, Thermo Scientific ...
more infohttps://www.alfa.com/en/ultrapure_biochemicals/

4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral...4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral...

The total cellular RNA was pelleted by centrifugation and redissolved in diethyl pyrocarbonate (DEPC-) treated . The ...
more infohttps://www.hindawi.com/journals/ecam/2013/435916/

Modeling of a mutation responsible for human 3-hydroxy-3-methylglutaryl-CoA lyase deficiency implicates histidine 233 as an...Modeling of a mutation responsible for human 3-hydroxy-3-methylglutaryl-CoA lyase deficiency implicates histidine 233 as an...

... lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored by incubation with hydroxylamine. ... Diethyl Pyrocarbonate / pharmacology. Enzyme Inhibitors / pharmacology. Histidine / metabolism*. Humans. Hydrolysis. Metabolism ... 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored ... 0/Acyl Coenzyme A; 0/Enzyme Inhibitors; 1553-55-5/3-hydroxy-3-methylglutaryl-coenzyme A; 1609-47-8/Diethyl Pyrocarbonate; 71-00 ...
more infohttp://www.biomedsearch.com/nih/Modeling-mutation-responsible-human-3/8798725.html

The effect of chemical modification of basic amino acid residues on the activation and amidolytic activity of Hageman factor ...The effect of chemical modification of basic amino acid residues on the activation and amidolytic activity of Hageman factor ...

Diethyl Pyrocarbonate / pharmacology. Factor XII / antagonists & inhibitors, chemistry*, metabolism. Factor XIIa / metabolism. ... 0/Amides; 0/Amino Acids; 0/Serum Albumin, Bovine; 1074-12-0/Phenylglyoxal; 1609-47-8/Diethyl Pyrocarbonate; 2508-19-2/ ...
more infohttp://www.biomedsearch.com/nih/effect-chemical-modification-basic-amino/8245689.html

Plus itPlus it

... diethyl pyrocarbonate; LRP, lung resistance-related protein; BCRP, breast cancer resistance protein; ABC, ATP-binding cassette ...
more infohttp://clincancerres.aacrjournals.org/content/9/2/827

Nuclease-Free Water at Your Fingertips | Sigma-AldrichNuclease-Free Water at Your Fingertips | Sigma-Aldrich

Diethyl pyrocarbonate - Product information sheet; /content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/d5758pis.pdf ... Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this ... A Mechanism of the Irreversible Inactivation of Bovine Pancreatic Ribonuclease by Diethylpyrocarbonate, European Journal of ...
more infohttps://www.sigmaaldrich.com/technical-documents/articles/biology/water-purification-systems/biomedical-applications/nuclease-free-water.html

Prevalence of dengue and chikungunya virus infections in north-eastern Tanzania: a cross sectional study among participants...Prevalence of dengue and chikungunya virus infections in north-eastern Tanzania: a cross sectional study among participants...

RNA was eluted from the silica pellets in 50 μl of diethyl pyrocarbonate (DEPEC) treated water and incubated for 10 min at 56 ° ...
more infohttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-016-1511-5

Enzymic characterization with progress curve analysis of a collagen peptidase from an enthomopathogenic bacterium, Photorhabdus...Enzymic characterization with progress curve analysis of a collagen peptidase from an enthomopathogenic bacterium, Photorhabdus...

Abbreviations used: AMC, 7-amino-4-methylcoumarin; But, t-butyl; DEPC, diethyl pyrocarbonate; DMF, dimethylformamide; DTT, ...
more infohttp://www.biochemj.org/content/379/3/633

Preparation of Short Interfering RNA Containing the Modified Nucleosides 2‐Thiouridine, Pseudouridine, or Dihydrouridine -...Preparation of Short Interfering RNA Containing the Modified Nucleosides 2‐Thiouridine, Pseudouridine, or Dihydrouridine -...

Diethyl pyrocarbonate (DEPC; Aldrich). * Milli‐Q water. * Automated oligonucleotide synthesizer (Applied Biosystems 394 or ...
more infohttp://www.currentprotocols.com/WileyCDA/CPUnit/refId-nc1602.html

Subtracting additives |  Michigan TodaySubtracting additives | Michigan Today

Diethyl pyrocarbonate (DEPC) - preservative (beverages). Carcinogen (1972). Dulcin (p-ethoxy-phenylurea) - artificial sweetener ...
more infohttps://michigantoday.umich.edu/2018/03/19/subtracting-additives/

Mechanism of Resistance to Dietary CholesterolMechanism of Resistance to Dietary Cholesterol

Total RNA was resuspended in diethyl pyrocarbonate-treated water. Twenty micrograms of RNA was then DNAse-treated using the ...
more infohttps://www.hindawi.com/journals/jl/2011/101242/

Synthesis of Site‐Specifically Phosphate‐Caged siRNAs - Current ProtocolsSynthesis of Site‐Specifically Phosphate‐Caged siRNAs - Current Protocols

Diethyl pyrocarbonate (DEPC) treated water (mix 1:1000 DEPC/HPLC‐grade water; sterilize at high temperature) ...
more infohttp://www.currentprotocols.com/WileyCDA/CPUnit/refId-nc0612.html?quicktabs_cp=toc

RNA Protocols & Applications - QIAGENRNA Protocols & Applications - QIAGEN

Alternatively, glassware can be treated with DEPC (diethyl pyrocarbonate). Fill glassware with 0.1% DEPC (0.1% in water), ...
more infohttps://www.qiagen.com/kr/resources/molecular-biology-methods/rna/

RNA Protocols & Applications - QIAGENRNA Protocols & Applications - QIAGEN

Alternatively, glassware can be treated with DEPC (diethyl pyrocarbonate). Fill glassware with 0.1% DEPC (0.1% in water), ...
more infohttps://www.qiagen.com/ke/resources/molecular-biology-methods/rna/

Plus itPlus it

... of random primer and diethyl pyrocarbonate-treated water to total volume 25 μl, then heated the mixture at 65°C for 5 min and ... and 6 μl of diethyl pyrocarbonate-treated water. The samples were incubated at 42°C for 2 min. Then 1 μl of Superscript II (40 ...
more infohttp://clincancerres.aacrjournals.org/content/10/15/4944

Purification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase.  - PubMed - NCBIPurification and characterization of the maize amyloplast stromal 112-kDa starch phosphorylase. - PubMed - NCBI

SP activity was inhibited by thioreactive agents, diethyl pyrocarbonate, phenylglyoxal, and ADP-glucose. The activation ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/11361132?dopt=Abstract

Different Roles of EIIABMan and EIIGlc in Regulation of Energy Metabolism, Biofilm Development, and Competence in Streptococcus...Different Roles of EIIABMan and EIIGlc in Regulation of Energy Metabolism, Biofilm Development, and Competence in Streptococcus...

RNA was resuspended in 52 μl of diethyl pyrocarbonate-treated water; 1 μl was used to estimate RNA concentration, and 1 μl was ... Pelleted cells were resuspended in 400 μl of diethyl pyrocarbonate-treated water, 800 μl of RNA protect reagent (QIAGEN, Inc., ...
more infohttps://jb.asm.org/content/188/11/3748?ijkey=55e929046a845023f3f2882e5a9eae31f709fabd&keytype2=tf_ipsecsha